2025/03/27 更新

写真a

シミズ ソノコ
清水 園子
Shimizu Sonoko
所属
イノベーション創出院 南予水産研究センター 准教授
職名
准教授
連絡先
メールアドレス
外部リンク

学位

  • 博士(農学) ( 九州大学大学院 )

研究キーワード

  • 赤潮

  • 魚病

  • 養殖環境

研究分野

  • 人文・社会 / 地域研究  / 地域連携

  • ライフサイエンス / 水圏生産科学

所属学協会

  • 日本水産増殖学会

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  • 環境ホルモン学会 [正式名称;日本内分泌撹乱化学物質学会]

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  • 日本水産学会

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論文

  • Spatio-temporal dynamics of the harmful dinoflagellate Karenia mikimotoi in Uwajima Bay and its adjacent waters: Comparison between a bloom occurrence year and a non-occurrence year

    GOH ONITSUKA, KENJI SUZUKAWA, NAOKI YOSHIE, MAKIKO HIRAI, SHOICHI TAKENAKA, YUUSAKU YOSHIHARA, HIDEJIRO OHNISHI, SONOKO SHIMIZU, HISATO TAKEUCHI, KOHEI OHTA, YUJI TOMARU, SETSUKO SAKAMOTO, KAZUO ABE, AKIRA YAMAGUCHI, TOMOYUKI SHIKATA, HITOMI YAMAGUCHI, HIDETAKA TAKEOKA

    Nippon Suisan Gakkaishi (Japanese Edition)   87 ( 2 )   144 - 159   2021年3月

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    掲載種別:研究論文(学術雑誌)  

    To elucidate the spatio-temporal dynamics of the harmful dinoflagellate Karenia mikimotoi and the relationship with environmental conditions in Uwajima Bay and its adjacent waters, we conducted field observations and analyzed the data in 2018 when a bloom occurred and in 2019 when it did not occur. In 2018, cell density of K. mikimotoi increased in Uwajima Bay, and then the bloom corresponding to low salinity water expanded to a broad area of the adjacent waters, suggesting that the spatio-temporal dynamics of the species were controlled by fresh water and nutrient discharges from rivers, and by passive transport due to a surface residual current. In 2019, although vegetative cells overwintered at low density, they did not form a bloom, possibly due to the environmental conditions for their growth, a large-scale intrusion of warm water (kyucho), and the existence of competing species.

    DOI: 10.2331/SUISAN.20-00055

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  • Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver

    Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Kayoko Ohta, Yoshitaka Nagahama, Kohei Ohta

    Cell Death and Differentiation   2020年

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    掲載種別:研究論文(学術雑誌)  

    © 2020, The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare. Autophagy, or cellular self-digestion, is an essential cellular process imperative for energy homeostasis, development, differentiation, and survival. However, the intrinsic factors that bring about the sex-biased differences in liver autophagy are still unknown. In this work, we found that autophagic genes variably expresses in the steroidogenic tissues, mostly abundant in liver, and is influenced by the individual’s sexuality. Starvation-induced autophagy in a time-dependent female-dominated manner, and upon starvation, a strong gender responsive circulating steroid-HK2 relation was observed, which highlighted the importance of estrogen in autophagy regulation. This was further confirmed by the enhanced or suppressed autophagy upon estrogen addition (male) or blockage (female), respectively. In addition, we found that estrogen proved to be the common denominator between stress management, glucose metabolism, and autophagic action in female fish. To understand further, we used estrogen receptor (ER)α- and ER-β2-knockout (KO) medaka and found ER-specific differences in sex-biased autophagy. Interestingly, starvation resulted in significantly elevated mTOR transcription (compared with control) in male ERα-KO fish while HK2 and ULK activation was greatly decreased in both KO fish in a female oriented fashion. Later, ChIP analysis confirmed that, NRF2, an upstream regulator of mTOR, only binds to ERα, while both ERα and ERβ2 are effectively pulled down the HK2 and LC3. FIHC data show that, in both ER-KO fish, LC3 nuclear-cytoplasmic transport and its associated pathways involving SIRT1 and DOR were greatly affected. Cumulatively, our data suggest that, ERα-KO strongly affected the early autophagic initiation and altered the LC3 nuclear-cytoplasmic translocation, thereby influencing the sex-biased final autophagosome formation in medaka. Thus, existence of steroid responsive autophagy regulatory-switches and sex-biased steroid/steroid receptor availability influences the gender-skewed autophagy. Expectedly, this study may furnish newer appreciation for gender-specific medicine research and therapeutics.

    DOI: 10.1038/s41418-020-0567-3

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  • Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge 査読

    Reza Mohammad, Ali Noman, Mohapatra Sipra, Shimizu Sonoko, Kitamura Shin-Ichi, Harakaw Shogo, Kawakami Hidemasa, Nakayama Kei, Sawayama Eitaro, Matsubara Takahiro, Ohta Kohei, Chakraborty Tapas

    FISH & SHELLFISH IMMUNOLOGY   82   286 - 295   2018年11月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.fsi.2018.08.027

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  • Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major) 査読

    Sipra Mohapatra, Tapas Chakraborty, Mohammad Ali Noman Reza, Sonoko Shimizu, Takahiro Matsubara, Kohei Ohta

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   206   42 - 53   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. Thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in Edwardsiella tarda infected red sea bream, we conducted a 20 day experiment using 4 treatment groups, namely, pre-fed placebo (PFP); pre-starved placebo (PSP); pre-fed infected (PFI); and pre starved infected (PSI), wherein a 5 h E. tarda infection was done on the 11th day. In the present investigation, the pre-starved groups showed significant (P < 0.05) alterations in the liver Hexokinase and Glucose-6-phosphatase activity. The pre-starved fish also exhibited significant (P < 0.05) increment in the hepatosomatic index, along with increased hepatic glycogen content, in a time dependent fashion. The PPAR (peroxisome proliferator activated receptors)ce transcription in the pre-starved group decreased significantly (P< 0.05) by 10dai, while the PPARy showcased a reverse pattern. The transcription of Hepcidinl and Transferrin (iron homeostasis related genes), and Cathepsin D and Ubiquitin (programmed cell death related genes) portrayed a time responsive decrease and increase in PSI and PFI groups, respectively. Additionally, in comparison to the PFI group, the PSI fish demonstrated substantially reduced oxidative stress level. Fluorescent Immunohistochemistry showed significant (P < 0.05) increase in p63 positive cells in the 10dai PFI fish in relation to the PSI group. Therefore, these findings provide new insight into the beneficial role of alternating starvation and refeeding schedule, preferably short-term starvation prior to an infection, in order to obtain better capability to battle against E. tarda infection in red sea bream. (C) 2017 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpb.2017.01.009

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  • Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major) 査読

    Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Shintaro Urasaki, Takahiro Matsubara, Yoshitaka Nagahama, Kohei Ohta

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY   189   1 - 10   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Dietary compromises, especially food restrictions, possess species-specific effects on the health status and infection control in several organisms, including fish. To understand the starvation-mediated physiological responses in Edwardsiella tarda infected red sea bream, especially in the liver, we performed a 20-day starvation experiment using 4 treatment (2 fed and 2 starved) groups, namely, fed-placebo, starved-placebo, fed-infected, and starved-infected, wherein bacterial exposure was done on the 11th day. In the present study, the starved groups showed reduced hepatosomatic index and drastic depletion in glycogen storage and vacuole formation. The fed-infected fish showed significant (P < 0.05) increase in catalase and superoxide dismutase activity in relation to its starved equivalent. Significant (P < 0.05) alteration in glucose and energy metabolism, as evident from hexoldnase and glucose-6-phosphate dehydrogenase activity, was recorded in the starved groups. Interestingly, coinciding with the liver histology, PPAR (peroxisome proliferator activated receptors) a transcription followed a time-dependent activation in starved groups while PPAR gamma exhibited an opposite pattern. The transcription of hepcidin 1 and transferrin, initially increased in Odai (days after infection) starved fish but reduced significantly (P < 0.05) at later stages. Two-color immunohistochemistry and subsequent cell counting showed significant increase in P63-positive cells at Odai and 5dai but later reduced slightly at 10dai. Similar results were also obtained in the lysosomal (cathepsin D) and non-lysosomal (ubiquitin) gene transcription level. All together, our data suggest that starvation exerts multidirectional responses, which allows for better physiological adaptations during any infectious period, in red sea bream. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2015.07.003

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  • 双方向通信を利用した赤潮予測のための「水産コミュニケーションシステム」開発に関する研究

    清水園子, 安藤顕人, 岡本拓也, 太田耕平, 黒田久泰, 樋上喜信, 遠藤慶一, 入野和朗, 吉田則彦, 浦崎慎太郎, 松原孝博, 小林真也

    平成28年度日本水産学会春季大会   2015年3月

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    記述言語:日本語   掲載種別:研究論文(研究会,シンポジウム資料等)  

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  • Tolerance of Spermatogonia to Oxidative Stress Is Due to High Levels of Zn and Cu/Zn Superoxide Dismutase 査読

    Fritzie T. Celino, Sonoko Yamaguchi, Chiemi Miura, Takashi Ohta, Yuzuru Tozawa, Toshiharu Iwai, Takeshi Miura

    PLOS ONE   6 ( 2 )   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: Spermatogonia are highly tolerant to reactive oxygen species (ROS) attack while advanced-stage germ cells such as spermatozoa are much more susceptible, but the precise reason for this variation in ROS tolerance remains unknown.
    Methodology/Principal Findings: Using the Japanese eel testicular culture system that enables a complete spermatogenesis in vitro, we report that advanced-stage germ cells undergo intense apoptosis and exhibit strong signal for 8-hydroxy-29-deoxyguanosine, an oxidative DNA damage marker, upon exposure to hypoxanthine-generated ROS while spermatogonia remain unaltered. Activity assay of antioxidant enzyme, superoxide dismutase (SOD) and Western blot analysis using an anti-Copper/Zinc (Cu/Zn) SOD antibody showed a high SOD activity and Cu/Zn SOD protein concentration during early spermatogenesis. Immunohistochemistry showed a strong expression for Cu/Zn SOD in spermatogonia but weak expression in advanced-stage germ cells. Zn deficiency reduced activity of the recombinant eel Cu/Zn SOD protein. Cu/Zn SOD siRNA decreased Cu/Zn SOD expression in spermatogonia and led to increased oxidative damage.
    Conclusions/Significance: These data indicate that the presence of high levels of Cu/Zn SOD and Zn render spermatogonia resistant to ROS, and consequently protected from oxidative stress. These findings provide the biochemical basis for the high tolerance of spermatogonia to oxidative stress.

    DOI: 10.1371/journal.pone.0016938

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  • Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel (Anguilla japonica) 査読

    Fritzie T. Celino, Sonoko Yamaguchi, Chiemi Miura, Takeshi Miura

    REPRODUCTION   138 ( 2 )   279 - 287   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOSCIENTIFICA LTD  

    The precise mechanism and direct effects of arsenic on fish, particularly in reproduction, are not well clarified. The aim of this study is to investigate the direct influence of arsenic on fish spermatogenesis using the Japanese eel (Anguilla japonica) in vitro testicular organ culture system. Eel testicular fragments were cultured in vitro with 0.1-100 mu M arsenic with or without human chorionic gonadotropin (hCG) for 6 or 15 days at 20 degrees C. Arsenic treatment provoked a dose-dependent inhibition of hCG-induced germ cell proliferation as revealed by 5-bromo-2-deoxyuridine immunohistochemistry. Time-resolved fluorescent immunoassay showed that arsenic suppressed hCG-induced synthesis of 11-ketotestosterone (11-KT) in testicular fragments incubated with 0.0001-100 mu M arsenic and hCG for 18 h. A 0.1 mu M (7 mu g/l) dose of arsenic which is lower than the World Health Organization drinking water quality guideline of 10 mu g/l most effectively reduced 11-KT production. The hCG-induced synthesis of progesterone from pregnenolone was significantly inhibited by low doses of arsenic (0.1-1 mu M), implying an inhibition of 3 beta-hydroxysteroid dehydrogenase activity. In situ TUNEL assays indicated that germ cells undergo apoptosis at the highest dose of arsenic (100 mu M). An arsenic concentration-dependent increase in oxidative DNA damage was detected by 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunohistochemistry. A peak in 8-OHdG index was observed in testicular fragments treated with 100 mu M arsenic and hCG consistent with the TUNEL results. These data suggest that low doses of arsenic may inhibit spermatogenesis via steroidogenesis suppression, while high doses of arsenic induce oxidative stress-mediated germ cell apoptosis. Reproduction (2009) 138 279-287

    DOI: 10.1530/REP-09-0167

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  • Zinc is an essential trace element for spermatogenesis 査読

    Sonoko Yamaguchi, Chiemi Miura, Kazuya Kikuchi, Fritzie T. Celino, Tetsuro Agusa, Shinsuke Tanabe, Takeshi Miura

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   106 ( 26 )   10859 - 10864   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Zinc (Zn) plays important roles in various biological activities but there is little available information regarding its functions in spermatogenesis. In our current study, we further examined the role of Zn during spermatogenesis in the Japanese eel ( Anguilla japonica). Human CG (hCG) was injected into the animals to induce spermatogenesis, after which the concentration of Zn in the testis increased in tandem with the progression of spermatogenesis. Staining of testicular cells with a Zn-specific fluorescent probe revealed that Zn accumulates in germ cells, particularly in the mitochondria of spermatogonia and spermatozoa. Using an in vitro testicular organ culture system for the Japanese eel, production of a Zn deficiency by chelation with N,N,N',N'-tetrakis (2-pyridylemethyl) ethylenediamine ( TPEN) caused apoptosis of the germ cells. However, this cell death was rescued by the addition of Zn to the cultures. Furthermore, an induced deficiency of Zn by TPEN chelation was found to inhibit the germ cell proliferation induced by 11-ketotestosterone (KT), a fish specific androgen, 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP), the initiator of meiosis in fish, and estradiol-17 beta (E2), an inducer of spermatogonial stem-cell renewal. We also investigated the effects of Zn deficiency on sperm motility and observed that TPEN treatment of eel sperm suppressed the rate and duration of their motility but that co-treatment with Zn blocked the effects of TPEN. Our present results thus suggest that Zn is an essential trace element for the maintenance of germ cells, the progression spermatogenesis, and the regulation of sperm motility.

    DOI: 10.1073/pnas.0900602106

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  • Novel expression of importin alpha homologue in marine teleost, Pagrus major 査読

    Koichiro Gen, Sonoko Yamaguchi, Koichi Okuzawa, Hirohiko Kagawa, Md. Samsul Alam

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   151 ( 4 )   420 - 427   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Importin alpha proteins are critical modulators of the classical nuclear protein import pathway. Although the physiological roles of importin alpha have been extensively studied in invertebrates and mammals, very little is known about their counterparts in lower vertebrates. In this study, to elucidate the roles of importin a in a teleost species, we isolated and characterized red seabream (Pagrus major) importin a cDNA derived from ovary and found changes in the mRNA levels of importin alpha in male and female red seabream during sexual maturation. The 1846-bp cDNA encodes a 520 amino acid protein that includes the importin 3-binding domain, a short acidic domain, and an armadillo (arm) repeat domain. Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) showed transcription of red seabream importin a in testis and ovary but not in the other tissues. The importin a mRNA levels in males increase in association with testicular development, whereas those in females remain high throughout sexual maturation. These findings suggest that red seabream ovary-derived importin a may be controlled in a tissue-specific manner and may perform unique functions in the gonad in addition to its involvement in nuclear transport. (C) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpb.2008.08.010

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  • Effects of arsenic on gonadal development in freshwater crab, Somanniathelphusa pax, in Vietnam and Geothelphusa dehaani in Japan 査読

    Sonoko Yamaguchi, Fritzie Tuble Celino, Aki Ito, Tetsuro Agusa, Shinsuke Tanabe, Bui Cach Tuyen, Chiemi Miura, Takeshi Miura

    ECOTOXICOLOGY   17 ( 8 )   772 - 780   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    To estimate the influence of water contamination by arsenic (As) on reproduction of crustaceans in Vietnam, we collected wild freshwater crab Somanniathelphusa pax from the Mekong Delta area in Vietnam, investigated gonadal development, and measured As concentration in hepatopancreas. In female crab, vitellogenesis was delayed in association with the increase of As accumulation in hepatopancreas, whereas there was no significant correlation between testicular development and As accumulation in male crab. To clarify the effects of As on gonadal development of crustaceans, we investigated the effects of oral As administration on gonadal development in Japanese freshwater crab Geothelphusa dehaani. In male crab, the occurrence of spermatids and spermatozoa were predominantly observed in the control group, whereas the occurrence of spermatocytes increased after administration of 10 mu g/crab As for 3 months. On the other hand, in females, secondary yolk globule stages mainly occupied ovary of the control group. However, the primary yolk globule stage gradually increased after 10 mu g/crab As administration. Together these results indicate that it is possible that As contamination in water or food causes the delay of spermatogenesis and vitellogenesis in crustaceans.

    DOI: 10.1007/s10646-008-0228-7

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  • Japanese Eel Follicle-Stimulating Hormone (Fsh) and Luteinizing Hormone (Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology 査読

    Yukinori Kazeto, Mayuko Kohara, Takeshi Miura, Chiemi Miura, Sonoko Yamaguchi, John M. Trant, Shinji Adachi, Kohei Yamauchi

    BIOLOGY OF REPRODUCTION   79 ( 5 )   938 - 946   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    Two gonadotropins (Gths), follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), control gonadal steroidogenesis and gametogenesis in vertebrates, including teleost fish. Here, we report on the production of biologically active recombinant Fsh (rec-Fsh) and Lh (rec-Lh) in Japanese eel using Drosophila S2 cells. The three subunits composing Gths, i.e., glycoprotein hormone, alpha polypeptide (Cga), follicle-stimulating hormone, beta polypeptide (Fshb), and luteinizing hormone, beta polypeptide (Lhb), were at first independently produced and were proven to be glycosylated and secreted as the mature peptides. Each beta subunit, along with its Cga, was simultaneously coexpressed to produce heterodimeric rec-Fsh and rec-Lh that were subsequently highly purified. The biological activity of rec-Gths was demonstrated in various in vitro assays. The rec-Gths differentially activated their receptors, which resulted in an increase in 11-ketotestosterone (11KT) secretion, a differential alteration of gene expression of steroidogenic enzymes in immature testis, and the induction of the complete process of spermatogenesis in vitro. The data strongly suggest that Fsh and Lh differentially play important roles in the reproductive physiology of the Japanese eel. By contrast, these rec-Gths exhibited little activity in the gonad when administered in vivo. This difference between in vitro and in vivo bioactivity is probably due to the qualitative nature of glycosylation in S2 cells, which resulted in degradation of the recombinant protein in vivo. These differences in the carbohydrate moieties need to be elucidated and ameliorated.

    DOI: 10.1095/biolreprod.108.070052

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  • Effects of trace elements on spermatogenesis and androgen production in fish 査読

    Sonoko Yamaguchi, Chiemi Miura, Fritzie Tuble Celino, Takeshi Miura

    CYBIUM   32 ( 2 )   165 - 165   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC FRANCAISE D ICHTYOLOGIE  

    The effects of Ph, Mo, As and Rb on spermatogenesis were investigated by using testicular organ culture of Japanese eel (Anguilla japonica). Treatment with these trace elements together with 11-ketotestosterone (KT) significantly inhibited KT-induced germ cells proliferation, suggesting that Mo, Rb, Ph and As directly interrupt KT-induced spermatogenesis. We also investigated the effects of As on in vitro KT synthesis in testis. KT production induced by human chorionic gonadotropin (hCG) was inhibited by low doses of As. This result suggested that As also may directly inhibit KT production.

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  • Effects of lead, molybdenum, rubidium, arsenic and organochlorines on spermatogenesis in fish: Monitoring at Mekong Delta area and in vitro experiment 査読

    Sonoko Yamaguchi, Chiemi Miura, Aki Ito, Tetsuro Agusa, Hisato Iwata, Shinsuke Tanabe, Bui Cach Tuyen, Takeshi Miura

    AQUATIC TOXICOLOGY   83 ( 1 )   43 - 51   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    To stimate the influence of water contaminants on fish reproduction in the Mekong Delta area, we sampled cultivated male catfish (Pangasianodon hypophthalmus), investigated testicular development, and measured persistent organic pollutants (POPs) and trace element levels in muscle and liver, respectively. Various testes sizes were observed although sampling took place during a short period. Histological analysis revealed that all developmental stages of germ cells were observed in catfish with large testis, whereas only necrotic spermatogonia but no other germ cells were observed in catfish with small testis. In small testis, furthermore, vacuolization and hypertrophy of Sertoli cells were observed. Measurement of POPs in muscle and trace elements in liver demonstrated that there were negative correlations between GSI and the concentrations of Pb, Mo, Rb and As. To clarify possible direct effects of Pb, Mo, Rb and As on spermatogenesis in fish, we investigated the effects of these trace elements on spermatogenesis using in vitro testicular organ culture of Japanese eel (Anguilla japonica). Treatment with each of the trace elements alone did not affect spermatogenesis. However, treatment with 10(-7) M of Pb, 10(-5) and 10(-4) M of Mo, 10(-5) -10(-3) M of Rb or 10(-5) M of As inhibited the spermatogenesis induced by 11-ketotestosterone (11 KT). Furthermore, treatment with 10(-4) M of As in combination with 11KT caused necrosis of testicular fragments. Taken together, these results are consistent with the hypothesis that Ph, Mo, Rb and As can exert inhibitory effects on spermatogenesis in catfish inhabiting the Mekong Delta area. (C) 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.aquatox.2007.03.010

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  • Roles of 11 beta-hydroxysteroid dehydrogenase in fish spermatogenesis 査読

    Yuichi Ozaki, Masato Higuchi, Chiemi Miura, Sonoko Yamaguchi, Yuzuru Tozawa, Takeshi Miura

    ENDOCRINOLOGY   147 ( 11 )   5139 - 5146   2006年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ENDOCRINE SOC  

    In fish spermatogenesis, the main action of progestins is generally regarded as the induction of sperm maturation. Our previous in vitro study demonstrated that a progestin, 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP), induced the initiation of meiosis in spermatogenesis in the Japanese eel (Anguilla japonica). In the present study, to elucidate the molecular mechanisms underlying the action of DHP, we attempted to clone cDNAs encoding genes whose expression was induced by DHP in eel testis, using cDNA subtraction. One of the cDNAs we isolated encodes eel 11 beta-hydroxysteroid dehydrogenase short form (e11 beta-HSDsf), and Northern blot and RT-PCR analysis showed that transcripts of e11 beta-HSDsf in testis were induced by DHP. The recombinant e11 beta-HSDsf had 11 beta-dehydrogenase activity, metabolizing cortisol to cortisone, and 11 beta-hydroxytestosterone to 11-ketotestosterone (11-KT). In vitro experiments revealed that eel immature testis had 11 beta-dehydrogenase activity, and DHP treatment enhanced the activity. To understand the role of 11 beta-HSD in spermatogenesis, we examined the direct effects of cortisol on eel spermatogenesis using an organ culture system. Cortisol induced DNA replication in spermatogonia and enhanced the spermatogonial proliferation induced by 11-KT. However, excess cortisol inhibited proliferation. In addition, 11-KT production was induced in testicular fragments incubated with cortisol. These results suggest that optimal levels of cortisol induced spermatogonial mitosis by increasing 11-KT production. Furthermore, two possible roles of DHP on spermatogenesis, via the up-regulation of 11 beta-HSD expression, are suggested: positive feedback control of 11-KT production and the modulation of cortisol levels to protect testes from excess circulating cortisol.

    DOI: 10.1210/en.2006-0391

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  • Influence of estradiol-17 beta, testosterone, and 11-ketotestosterone on testicular development, serum steroid hormone, and gonadotropin secretion in male red sea bream Pagrus major 査読

    Sonoko Yamaguchi, Koichiro Gen, Koichi Okuzawa, Michiya Matsuyama, Hirohiko Kagawa

    FISHERIES SCIENCE   72 ( 4 )   835 - 845   2006年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    In order to investigate the influence of estrogen and androgen on reproductive activities of male teleosts, male red sea bream were implanted with silicone capsules containing estradiol-17 beta (E2), testosterone (T) or 11-ketotestosterone (11-KT) in immature and early spermatogenic stages. One month after implantation of either E2 or T, the gonadosomatic index decreased in accordance with testicular regression in both stages. Implantation of E2 decreased circulating 11-KT levels but did not affect gonadotropin (GTH) subunits, follicle stimulating hormone-beta (FSH beta), luteinizing hormone-beta (LH beta), a glycoprotein subunit (alpha GSU) gene expression, and serum LH levels in both stages. Alternatively, T decreased serum 11-KT and LH levels, and FSH beta and LH beta mRNA levels in the early spermatogenic stage but not in the immature stage. These results suggest E2 may directly inhibit testicular development through the suppression of 11-KT production. Meanwhile, T may decrease serum 11-KT levels through the suppression of FSH and LH secretion, resulting to inhibition of testicular development in the early spermatogenic stage. Treatment with 11-KT did not affect the testis in either stage, whereas 11-KT increased LH beta and alpha GSU mRNA levels in immature, and decreased FSH beta mRNA levels in the early spermatogenic stage. These results suggest that 11-KT may have different effects on GTH subunit gene expression in each reproductive stage.

    DOI: 10.1111/j.1444-2906.2006.01225.x

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  • The role of high water temperature in the termination of spawning of red seabream 査読

    Koichi Okuzawa, Naoki Kumakura, Koichiro Gen, Sonoko Yamaguchi, Bong-Soo Lim, Hirohiko Kagawa

    ZOOLOGICAL SCIENCE   21 ( 12 )   1336 - 1337   2004年12月

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    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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  • Physiological roles of FSH and LH in red seabream, Pagrus major

    Koichiro Gen, Sonoko Yamaguchi, Koichi Okuzawa, Naoki Kumakura, Hideki Tanaka, Hirohiko Kagawa

    Fish Physiology and Biochemistry   28 ( 1-4 )   77 - 80   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The duality of gonadotropins (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), has been confirmed in most teleost species, but very little is known about their biological functions. To elucidate the physiological roles of FSH and LH in fish reproduction, the expression profiles of GTH subunit genes during gonadal development were analyzed in both male and female red seabream. Furthermore, in vitro studies were carried out to examine the effects of GTHs on steroid hormone production and cytochrome P450 aromatase (P450arom) expression in red seabream gonads. In both sexes, LHβ mRNA was maintained at high levels from the early gametogenesis until spawning season, and declined with gonadal regression. Interestingly, FSHβ mRNA levels in males increased in parallel with testicular development, whereas those in female were remained low throughout oocyte development. From in vitro studies using purified red seabream FSH and LH, both GTHs had a similar potency in stimulating 11-ketotestosterone production by testicular slices, while the biological activity of FSH was much lower than that of LH in stimulating production of estradiol-17β by vitellogenic follicles. Moreover, expression of P450arom mRNA was induced by LH, but not FSH, in ovarian follicles in vitro. FSH was also ineffective in inducing maturational competence and final oocyte maturation. These results suggest that, unlike salmonids, FSH may play an important role during gametogenesis in male, but not female, red seabream, whereas LH may be involved in regulation of both early and late gametogenesis in both sexes. © 2004 Kluwer Academic Publishers.

    DOI: 10.1023/B:FISH.0000030480.97947.ba

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  • Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro

    Kohei Ohta, Sonoko Yamaguchi, Akihiko Yamaguchi, Koichiro Gen, Koichi Okuzawa, Hirohiko Kagawa, Michiya Matsuyama

    Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   133 ( 1 )   45 - 54   2002年9月

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    掲載種別:研究論文(学術雑誌)  

    The steroid synthesis pathway in the ovarian follicles of the red seabream during final oocyte maturation (FOM) was investigated by incubating intact follicles with different radioactively labeled steroid precursors. During FOM, the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred mainly due to a combination of inactivation of C17,20-lyase and activation of 20β-hydroxysteroid dehydrogenase. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro. 17,20β-P was further converted to its 5β-reduced form, 17,20β-dihydroxy-5β-pregnan-3-one (17,20β-P-5β), which had lower GVBD activity, suggesting that 5β-reduction plays a role in the inactivation of the maturation-inducing ability of 17,20β-P. In contrast, no 5β-reduced metabolite of 20β-S was found. Serum levels of 17,20β-P and 20β-S, measured by ELISA, showed that circulating levels of both progestins increased during FOM, and 20β-S levels were approximately twice as high as 17,20β-P levels. This study clarified the complete steroidogenesis pathway during FOM in red seabream ovarian follicles and showed that two 20β-hydroxylated progestins, 17,20β-P and 20β-S, act as maturation-inducing hormones in this species. The catabolites of these two progestins and their physiological roles in reproduction are also discussed. © 2002 Elsevier Science Inc. All rights reserved.

    DOI: 10.1016/S1096-4959(02)00106-9

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  • Biosynthesis of estradiol-17β in the ovarian follicles of the red seabream Pagrus major during vitellogenesis

    Kohei Ohta, Sonoko Yamaguchi, Akihiko Yamaguchi, Koichi Okuzawa, Koichiro Gen, Hirohiko Kagawa, Michiya Matsuyama

    Fisheries Science   68 ( 3 )   680 - 687   2002年

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    掲載種別:研究論文(学術雑誌)  

    The red seabream Pagrus major is a useful experimental fish for studying the endocrine control of oogenesis in teleosts. This study investigated the steroidogenic pathway for estradiol-17β (E2) biosynthesis in the ovarian follicles of red seabream. Intact follicles were isolated during vitellogenesis and incubated in vitro with different radiolabeled steroid precursors. When 17-hydroxyprogesterone (17-P), dehydroepiandrosterone (DHEA), or androstenedione (AD) were used as precursors, both testosterone (T) and estrone (E1) were synthesized by follicles, leading to estradiol-17β (E2) production. Serum steroid levels measured by enzyme-linked immunosorbent assay showed that T, E1, and E2 were present in the circulation at levels ranging from 1 ng/mL to 2 ng/mL throughout the day during the spawning season. In vitro conversion of E1 into E2, however, was 15.8-fold greater than T conversion into E2, suggesting that E2 is synthesized mainly via E1 rather than T. The results showed that E2 was synthesized from pregnenolone via 17-hydroxypregnenolone, DHEA, AD, and E1. Thus, the study demonstrated the complete steroidogenic E2 synthesis pathway in the ovarian follicles of red seabream, and revealed that E1 is the major precursor of E2.

    DOI: 10.1046/j.1444-2906.2002.00477.x

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  • Effects of gonadal steroids on gonadotropin subunit genes expression in male red seabream (Pagrus major) 査読

    Sonoko Yamaguchi, Koichiro Gen, Koichi Okuzawa, Michiya Matsuyama, Hirohiko Kagawa

    FISHERIES SCIENCE   68   953 - 954   2002年

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    記述言語:英語   出版者・発行元:SPRINGER TOKYO  

    DOI: 10.2331/fishsci.68.sup1_953

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MISC

講演・口頭発表等

  • 養殖現場と連携した双方向『水産情報コミュニケーション システム』による赤潮・魚病対策技術の開発 招待

    清水 園子

    一次産業×ICTに関する技術セミナー :ドローン・IoT利活用による有害生物対策とエコシステム  2016年6月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • Edwardsiella tardaの早期検出法および海水中の動態

    原川翔伍, 川上秀昌, 清水園子, 太田耕平

    平成29年度日本魚病学会秋季大会  2017年9月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • マダイのエドワジエラ症感染早期検出技術の開発

    松本真依, Sipra Mohapatra, Tapas Chakraborty, 川上秀昌, 原川翔悟, Reza Mohammad Ali Noman, 太田耕平, 松原孝博, 清水園子

    平成29年度日本増殖学会第16回大会  2017年11月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • 魚病早期検出のための海水中病原体の挙動解析

    服部純也, 岡本渉, 齋藤類, 太田耕平, 松原孝博, 武岡英隆, 清水園子

    平成29年度日本増殖学会第16回大会  2017年11月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • Characterization of complement system association in stress responsive immunity modulation in red sea bream, Pagrus major

    Mohammad Ali Noman Reza, Tapas Chakraborty, Sipra Mohapatra, Shogo Harakawa, Hidemasa Kawakami, Takahiro Matsubara, Kohei Ohta, Sonoko Shimizu

    平成29年度日本増殖学会第16回大会  2017年11月 

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    記述言語:英語   会議種別:ポスター発表  

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  • 赤潮発生予測を目指した有害赤潮プランクトンの挙動解析

    岡本渉, 服部純也, 齋藤類, 太田耕平, 松原孝博, 武岡英隆, 清水園子

    平成29年度日本増殖学会第16回大会  2017年11月 

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    記述言語:日本語  

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  • マダイエドワジエラ症早期検出のための 血液中マイクロRNAの探索

    清水園子, Tapas Chakraborty, 竹内久登, 川上秀昌, 原川翔伍, Sipra Mohapatra, 太田耕平, 松原孝博

    2019年度日本水産学会秋季大会  2019年9月 

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    記述言語:日本語  

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  • 双方向通信を利用した赤潮予測のための「水産コミュニケーションシステム」開発に関する研究

    清水園子, 安藤顕人, 岡本拓也, 太田耕平, 黒田久泰, 樋上喜信, 遠藤慶一, 入野和朗, 吉田則彦, 浦崎慎太郎, 松原孝博, 小林真也

    平成28年度日本水産学会春季大会  2016年3月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • Effects of trace elements on spermatogenesis in fish 国際会議

    Yamaguchi, Sonoko, Celino, F. T, Miura, C, Miura, T

    International symposium. Pioneering Studies of Young Scientists on Chemical Pollution and Environmental Changes  2006年11月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Effects of Zinc on spermatogenesis in Japanese eel (Anguilla japonica). 国際会議

    2008年5月 

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    記述言語:英語   会議種別:ポスター発表  

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  • 養殖海域における魚病早期発見のための病原体高感度早期検出法の確立

    清水園子, 田中俊也, 浦崎慎太郎, 山下亜純, 松原孝博, 太田耕平

    平成26年度日本魚病学会秋季大会  2014年9月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • BENEFITS OF STARVATION IN DISEASE MANAGEMENT IN RED SEA BREAM (Pagrus major) AQUACULTURE 国際会議

    Aquaculture 2016  2016年2月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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共同研究・競争的資金等の研究課題

  • 層状複水酸化物を用いた赤潮プランクトン除去に関する研究

    2022年4月 - 2025年3月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    福垣内 暁, 清水 園子

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    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

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  • 魚類の健康コンディション評価のための血液中ノンコーディングRNA網羅的解析

    2016年4月 - 2019年3月

    日本学術振興会  科研費 若手研究(B) 

    清水 園子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 養殖現場と連携した双方向『水産情報コミュニケーションシステム』による赤潮・魚病対策技術の開発の研究開発

    2015年4月 - 2018年3月

    総務省  戦略的情報通信研究開発推進事業(SCOPE)【地域ICT振興型研究開発】 

    清水 園子

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    担当区分:研究代表者  資金種別:競争的資金

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  • ICTを利用した養殖魚の感染性疾病予防システム構築のための基盤研究

    2015年4月 - 2018年3月

    農林水産省  農林水産業・食品産業科学技術研究推進事業【発展融合ステージ】重要施策対応型 

    清水 園子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 養殖魚における餌止め効果のメカニズム:なぜ、赤潮・魚病による斃死を軽減できるか?

    2011年4月 - 2014年3月

    日本学術振興会  科学研究費助成事業  若手研究(A)

    太田 耕平, 松原 孝博, 浦﨑 慎太郎, 清水 園子, 鎌田 幸介, 向井 幸樹, 田中 俊也, 北川 雄也

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    配分額:26000000円 ( 直接経費:20000000円 、 間接経費:6000000円 )

    魚類養殖を効率的かつ安定的に行う上で、赤潮と魚病による斃死が最大のネックとなる。これらに対して、現場では日常的に餌止めが行われているが、科学的情報が不足しているため、適切な餌止めが困難である。そこで、餌止めによる生体防御効果(絶食効果)の科学的解明を目的とした。
    ブリおよびマダイを用いて解析した結果、絶食に伴い呼吸量や血中グルコース量などに加え、特定のアミノ酸濃度、さらに各臓器のATP量が減少した。一方、感染時の各種免疫・代謝関連因子は絶食により上昇した。赤潮暴露では酸欠症状がおこり、呼吸量低下が抵抗性を高める一因と考えられたが、栄養成分低下に起因した興奮抑制効果など、複合的要因も示唆された。

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  • 環境汚染由来重金属が魚類生殖に及ぼす毒性メカニズムの解明

    2006年4月 - 2009年3月

    日本学術振興会  科研費 特別研究員奨励費 

    山口園子

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    資金種別:競争的資金

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担当授業科目(学内)

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