担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jphs.2022.08.002

PubMed

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bja.2021.04.009

PubMed

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bja.2021.04.009

PubMed

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fphar.2020.01004

PubMed

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0227582

PubMed

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1155/2019/7510214

PubMed

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bcp.2018.02.013

Scopus

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/srep28133

Web of Science

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.4049/jimmunol.1401976

Web of Science

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/icb.2014.45

Web of Science

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/icb.2011.41

Web of Science

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0319103

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.7759/cureus.57555

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Comprehensive profiling of the transcriptome reflects the dynamic changes in complex regulator interactions between genes and proteins in the pathological processes of autoimmune disease. To enable real-time adaptive sampling and run rapid sequencing with maximal expected readings of 50 Gb of data with accessibility to clinicians, basic researchers, and even students, a portable and affordable sequencing device, MinION, was employed in our laboratory for both basic and clinical studies. Here, the workflow of bulk RNA-seq in murine spleen using MinION is introduced. The methodology of both laboratory library preparation and the establishment of a bioinformatic pipeline are included in this chapter.

DOI： 10.1007/978-1-0716-3682-4_30

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The diagnosis of vasculitis in rheumatoid arthritis (RV) is associated with considerable mortality; therefore, understanding the basic mechanisms underlying the pathogenesis of vasculitis is very important. Animal models of vasculitis have contributed to elucidating such mechanisms. We here introduce a Candida albicans water-soluble glycoprotein (CAWS)-induced vasculitis model and the methodological approach to evaluate inflammatory vascular change.

DOI： 10.1007/978-1-0716-3682-4_28

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.

DOI： 10.1007/978-1-0716-3682-4_13

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

To protect subjects who participate in human research, Institutional Review Boards (IRBs) play an important role in reviewing research and determining the validity of a study by comprehensively examining it for ethical issues, including invasiveness and management of personal information. They conduct regular and independent reviews to protect the health, rights, and welfare of research subjects. When we as researchers conduct clinical research, we must obtain IRB approval and submit our research for investigation of ethical issues before we begin.

DOI： 10.1007/978-1-0716-3682-4_31

PubMed

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記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）   出版者・発行元：(NPO)日本高血圧学会  

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記述言語：日本語   出版者・発行元：(一社)日本女性栄養・代謝学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Frailty attracts research as it represents a significant target for intervention to extend the healthy life span. An unanswered question in this field is the time point during the life-course at which an individual becomes predisposed to frailty. Here, we propose that frailty has a fetal origin and should be regarded as part of the spectrum of the developmental origins of health and disease. The developmental origins of health and disease theory originated from findings linking the fetal environment to lifestyle-related disorders such as hypertension and diabetes. Coincidentally, a recent trend in frailty research also centers on vascular dysfunction and metabolic alterations as the causality of lifestyle-related disorders such as sarcopenia and dementia. Here, we explore the relationship between fetal programming, frailty-related disorders (sarcopenia and dementia), and other age-related diseases mainly based on reports on intrauterine growth restriction. We propose a "total" life-course approach to combat frailty. With this viewpoint, not only physicians and gerontologists but also obstetricians and pediatricians should team up to overcome age-related diseases in the elderly. Geriatr Gerontol Int 2023; 23: 263-269.

DOI： 10.1111/ggi.14565

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/ggi.14562

PubMed

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記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）   出版者・発行元：日本心脈管作動物質学会  

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：論文集(書籍)内論文  

DOI： 10.1007/16833_2022_108

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

The present study is undertaken to investigate the effect of fetal growth restriction (FGR) on retinal neovasculature in a murine premature neonatal oxygen-induced retinopathy (OIR) model. According to the results of histological analysis, a significant decrease of neovasculature, as indicated by the decreases in the number of branch junctions, the vesicular distribution, maximal vesicular radius and microaneurysm-like tufts, were observed in OIR mice with FGR while comparing to OIR neonates with normal birth weight. The results of retinal RNA-sequencing revealed a down-regulation of angiogenic factors that trigger pathologic retina neovascularization, such as MAPK pathway and relative upstream signaling pathways in OIR mice with FGR. These results suggest that FGR neonates may be equipped with a higher capacity for retinal oxygen stress, and the risk of OIR development is lower compared to mature neonates.

DOI： 10.1254/jpssuppl.97.0_1-b-p-022

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記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）   出版者・発行元：公益社団法人 日本薬理学会  

It has been known that sarcopenia causes the immune dysfunction. In this study, we evaluated the influence of the sarcopenia on the progression of melanoma using a denervation-induced sarcopenic model and the efficacy of leucine-intervention was evaluated. 

Initially, the influences of leucine-intake on maintaining immune homeostasis were observed. The expression of PD1 on CD4-positive T cells and CD8-positive T cells increased in the sarcopenia mice comparing those in control mice. Then, a diet-intake leucine was orally administrated to both sarcopenic mice and melanoma-implanted sarcopenic mice. The administration of leucine caused an elevation of total CD4-positive cells and significantly decreased the number of PD1+CD4+ fraction and PD1+CD8+ fraction in sarcopenic mice. The subpopulation of PD1+CD8+ fraction in leucine-treated sarcopenic mice was even much lower than that in non-sarcopenic group. The Kaplan-Meier curve also suggests that leucine-intake potentially increased the survival rates in sarcopenic mice. 

Taken together, the results obtained from the present study suggests that oral administration of leucine can restore the skeletal muscle mass and may affect the onset of cancer progression via improving the function of immunological defense. It would benefit the management of melanoma in sarcopenic patients.

DOI： 10.1254/jpssuppl.97.0_2-b-p-085

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記述言語：日本語   出版者・発行元：日本女性栄養・代謝学会  

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記述言語：日本語   出版者・発行元：(一社)日本老年医学会  

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Aiming at complete excision of cholesteatoma during trympanomastoidectomy and therefore reducing the risk of recurrence, the current study was undertaken to develop a seed DNA-aptamer-based fluorophore-probe, which targets human galectin-7, as an intraoperative lesion-identifying indicator for the surgical treatment of cholesteatoma. A galectin-7-targeted DNA-aptamer library was generated for labeling the cholesteatoma matrix using cell-based systematic evolution of ligands by an exponential enrichment technique. The binding characteristics of the identified aptamers were analyzed, and structure optimization of the identified aptamers was carried out both in silico and in vitro. Using galectin-7-aptamer guided molecular imaging, the excision margins of cholesteatoma matrix and surrounding normal tissue were successfully observed in a xenografted cholesteatoma model. It is highly expected that specific galectin-7-aptamers could progress to future clinical trials for both imaging and therapeutic applications and therefore benefit cholesteatoma patients.

DOI： 10.1254/jpssuppl.96.0_2-b-p-169

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

It is well known that chronic inflammation causes the sarcopenia. Whether the efforts to prevent skeletal muscle loss benefit the immune homeostasis remains unclear. In the present study, the efficacy of leucine supplementation on the maintenance of systemic immune function was evaluated. A sciatic nerve denervation-induced sarcopenic model was established and the volume and thickness of skeletal muscle on the hindlimb were evaluated by magnetic resonance imaging. Oral administration of leucine was carried on for 56 weeks. The skeletal muscle mass and the subsets or function of immune cells were analyzed. 

In leucine-treated sarcopenic mice, skeletal muscle mass on the hindlimb was significantly increased compared to that in non-treated sarcopenic mice. Leucine treatment repaired the mitochondria dysfunction in splenocytes from sarcopenic mice both <i>in vitro</i> and <i>in vivo</i>. In sarcopenic mice, an increase of the PD-1 expression was observed in CD4+ and CD8+ T cells. However, oral leucine administration restored the expression of PD-1 in the lymphocytes to the level of non-sarcopenic mice. 

In conclusion, the administration of leucine exhibits beneficial effects on sarcopenia and may influence the anti-cancer immune responses via adaptive immune resistance mechanism.

DOI： 10.1254/jpssuppl.96.0_2-b-ss11-2

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Mast cells are important sentinel cells in the first line of host defense against viral and bacterial entry. SARS-CoV-2 can activate mast cells present in the respiratory tract in the initial stage of the disease. In the present study, we attempted to dissect the mechanisms underlying the interaction of the function of mast cells, especially focusing on the mast cell-derived chymase, with the cell entry of SRAS-CoV-2 by using a well-established mast cell line, rat basophil leukemia (RBL-2H3) cells. RBL-2H3 cells were infected by pseudovirons and the viral entry was evaluated. The interactions between SRAS-CoV-2 spike and mast cell-derived chymase were observed and predicated binding-site were determined by using spike-truncating variants. During the viral infection, the interaction of chymase with SRAS-CoV-2 may have both detrimental and positive impacts. Whether SRAS-CoV-2 spike is a potential substrate of chymase, and if its cleavage modulates biological properties of spike-bearing virus would be focused on in future planned study.

DOI： 10.1254/jpssuppl.95.0_1-p-059

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1248/bpb.b21-00717

PubMed

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記述言語：日本語   出版者・発行元：(一社)日本サルコペニア・フレイル学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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掲載種別：論文集(書籍)内論文  

Allergic diseases, including allergic rhinitis and asthma, reduce the quality of life when severe. Allergic disease is conventionally characterized by an increase in serum immunoglobulin E and activation of type 2 helper T cells and eosinophils. There is accumulated evidence that Enterococcus faecalis, a kind of lactobacillus preparation, has a significant effect on various allergic diseases through regulation of the systemic immune response. Since E. faecalis is a gram-positive, facultative anaerobic organism and causes infectious diseases such as bacteremia, E. faecalis preparations have been investigated and developed as a tyndallized probiotic. In addition, tyndallized treatment with E. faecalis FK-23 augments the effect on health promotion. It has been reported that lysed E. faecalis FK-23 (LFK) as a tyndallized probiotic has health-promoting effects by activation of Treg cells. We also reported that oral administration of LFK provides therapeutic benefit in ovalbumin-induced asthma model mice through regulation of the Treg/Th17 balance. Many of the studies were conducted by a group at Nichinichi Pharmaceutical Co., Ltd. The effects of LFK preparation on allergic diseases are introduced in this article.

DOI： 10.1016/B978-0-12-819815-5.00032-X

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Patients with systemic autoimmune disease are predisposed to developing sarcopenia associated with their underlying proinflammatory condition and a decrease in motility. How sarcopenic status affects disease progression remains unknown. The present study explored the influence of sarcopenia on immune homeostasis and investigated related biological pathways that might be important for future disease management. A denervation-induced skeletal muscle loss model was established and the function of the immune system was evaluated. The results of immune status profiling and functional assessment demonstrated that the loss of skeletal muscle impaired the function of mitochondrial respiration and activation in T cells, and therefore influenced the balance of T helper cell subsets. Consequently, sarcopenia might promote the progression of autoimmune diseases, such as lupus nephritis, as well as the enhancement of Th1/Th17 functions. These results suggest that physicians should be aware of the impact of the loss of skeletal muscle on the management of autoimmune disease, and that a multidisciplinary approach is required to minimize the overall adverse impact of sarcopenia.

DOI： 10.1254/jpssuppl.94.0_1-p2-22

CiNii Research

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語  

DOI： 10.1016/j.bja.2020.05.046

PubMed

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記述言語：日本語   出版者・発行元：(一社)日本抗加齢医学会  

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ejphar.2020.173104

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

In atopic diseases, the epithelium releases cytokines and chemokines that initiate skin inflammation. Atopic dermatitis (AD) is characterized by a disrupted epidermal barrier and is triggered or exacerbated by environmental stimuli such as house dust mite (HDM) allergens. The proinflammatory cytokine interleukin 33 (IL-33) plays an important role in the pathogenesis of AD, but how IL-33 production in keratinocytes is elicited by HDM is unknown. To that end, here we stimulated monolayer-cultured human keratinocytes and human living skin equivalents with Dermatophagoides pteronyssinus HDM extract to investigate its effects on IL-33 production from keratinocytes. The HDM extract induced intracellular expression of IL-33 and modulated its processing and maturation, triggering rapid IL-33 release from keratinocytes. Group 1 HDM allergen but not group 2 HDM allergen elicited IL-33 production. An ATP assay of keratinocyte culture supernatants revealed an acute and transient accumulation of extracellular ATP immediately after the HDM extract stimulation. Using the broad-spectrum P2 antagonist suramin, the specific purinergic receptor P2Y2 (P2RY2) antagonist AR-C118925XX, and P2RY2-specific siRNA, we discovered that the HDM extract-induced IL-33 expression was mainly dependent on extracellular ATP/P2Y2 signaling mediated by transactivation of epidermal growth factor receptor, followed by activation of the ERK kinase signaling pathway. Moreover, HDM extract–induced release of 25-kDa IL-33 from the keratinocytes depended on an extracellular ATP/P2 signaling–mediated intracellular Ca2+ increase. Our study demonstrates the new mechanism controlling the induction and maturation of keratinocyte-produced IL-33 by HDM allergens, an innate immune process that might play a role in AD development or severity.

DOI： 10.1016/j.bbadis.2020.165719

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00011-020-01319-z

PubMed

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Defects in articular cartilage ultimately results in loss of joint function in rheumatoid arthritis. To investigate the influences of anti-rheumatoid drugs on chondrogenic differentiation of mesenchymal stem cell (MSC), <i>in vitro</i> and <i>in vivo</i> screening system were established. MSCs were collected and palleted in 4-microtube strips for chondrogenic induction. Quantification of formed micromasses was performed by three dimensional T2-weighted magnetic resonance imaging. For <i>in vivo</i> screening, scaffold or scaffoldless cartilaginous tissue were transplanted to NOD/ShiJic-scid mice for 2 months. The cartilaginous tissues were then explanted and the expressions of chondrogenic markers, including aggrecan and CD44, were assessed. We examined influence on inducted differentiation cartilages by methotrexate (MTX) and prednisolone (PSL). The volume of the chondrogenic spheroid in the presence of MTX was decreased in a dose-dependent manner, whereas no significant effect of PSL on chondrogenic differential potency were observed. The MSC-derived cartilaginous spheroid provides an effective screening tool to get the impact of anti-rheumatoid drugs on cartilaginous regeneration.

DOI： 10.1254/jpssuppl.93.0_1-ss-39

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

In present study, we aimed to investigate the feasibility of machine-learning-based classification using clinical features of patients for risk predication of anesthesia-related anaphylaxis.

After data pre-processing, the performance of four classification methods, which were integrated with four feature selection methods, were evaluated using two-layer cross-validation. Linear Discriminate Analysis in conjunction with Recursive Feature Elimination presented the best performance, with accuracy of 0.867 and Matthews correlation coefficient of 0.558 with 25 features used in the classification.

This study presents initial proof of the capability of a machine-learning-based strategy for forecasting low-prevalence anesthesia-related anaphylaxis. In future, we plan to utilize an extended database including preoperative information and vital-sign streams to define personalized risk status for anaphylaxis.

DOI： 10.1254/jpssuppl.93.0_3-p-385

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jphs.2019.03.007

PubMed

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記述言語：日本語   出版者・発行元：(一社)日本集中治療医学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ejphar.2019.01.050

PubMed

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Mas-related G protein coupled receptor-X2 (MRGPRX2) is a novel G protein-coupledreceptor with unique features including selective expression and response topeptidergic drug-induced activation in mast cells. With the aim of inhibitingMRGPRX2-dependent mast cell activation, a specific functional aptamer wasgenerated using the proteoliposome-based systematic evolution of ligands byexponential enrichment. After multiple cycles of selection and evolution,potential functional aptamers with high affinity and specificity were selectedby binding assay and functional screening and enriched for sequencing. Severalaptamers showed dose-dependent inhibitory potency on MRGPRX-dependent mast cellactivation. The feasibility and efficacy of these aptamers on drug-inducedanaphylactoid reactions will be investigated in our future planned study.

DOI： 10.1254/jpssuppl.92.0_3-p-104

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記述言語：日本語   出版者・発行元：公益社団法人 日本薬理学会  

Muscle atrophy is a result of aging and disease, and can compromise physical function and impair vital metabolic processes. Low levels of muscular fitness, known as ‘flail', contribute greatly to weakness, disability, and immobility, and lead to increased hospitalization and loss of independence.

To investigate the correlation between muscle atrophy and aging-related immune deterioration, a dissected sciatic nerve sarcopenic mouse model was established and the fraction of immunological cells, including T cells, B cells, macrophages, natural killer cells, and neutrophils, was determined. Eight weeks after sciatic nerve dissection, the volume of both hind legs was evaluated based on acquired magnetic resonance imaging. Primary splenocytes and biceps femoris muscle-derived cells were isolated and labeled by cellular markers. The subpopulation of the cells was then detected using flow cytometry. T and B cell lineages were significantly suppressed in the sarcopenic model compared with control mice, and the fraction of macrophages and natural killer cells also tended to increase.

In conclusion, muscular loss appears to affect the immunological system by modulating humoral immunity and the cell-based immunology response. Further functional analysis of individual cell subsets could further determine the influence of sarcopenia on immune system improvement.

DOI： 10.1254/jpssuppl.92.0_1-ss-54

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0198785

PubMed

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ejphar.2018.02.006

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fphar.2018.00038

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Humana Press Inc.  

The Ca 2+ ion is an important second messenger in lymphocytes, similarly to its function in other mammalian cells. The generation of long-lasting intracellular Ca 2+ elevations is essential for Ca 2+ -dependent gene transcription, proliferation, differentiation, and cytokine production in lymphocytes. Since store-operated Ca 2+ entry (SOCE) is considered the predominant mode of Ca 2+ influx in lymphocytes, the activation and function of lymphocytes can be generally predicted by monitoring SOCE. A method suitable for dynamic monitoring of Ca 2+ influx using fura-2 labeling in lymphocytes is introduced in this chapter. Using this technique, large-scale screening of the activation status of primary or cultured lymphocytes can be realized.

DOI： 10.1007/978-1-4939-8802-0_16

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DOI： 10.1007/978-1-4939-8802-0_9

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.

DOI： 10.1007/978-1-4939-8802-0_11

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掲載種別：論文集(書籍)内論文  

The diagnosis of vasculitis in rheumatoid arthritis (RV) is associated with considerable mortality; therefore, understanding the basic mechanisms underlying the pathogenesis of vasculitis is very important. Animal models of vasculitis have contributed to elucidating such mechanisms. We here introduce a Candida albicans water-soluble (CAWS) glycoprotein-induced vasculitis model and the methodological approach to evaluate inflammatory vascular change.

DOI： 10.1007/978-1-4939-8802-0_23

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Humana Press Inc.  

Since mice are widely used to establish rheumatoid arthritis models, assessment of the pathogenesis of local arthritis is fundamental. Proteins are the most diverse group of biologically important molecules and are essential for cellular structure and function. The first step in pathogenesis-related protein analysis is joint tissue extraction. Unlike other large rodents, obtaining synovium from model mice is challenging, since it is so small and fragile. In this chapter, methods for harvesting synovium through a quadriceps approach and preparing protein extracts are introduced.

DOI： 10.1007/978-1-4939-8802-0_6

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Humana Press Inc.  

To achieve the most accurate assessment of functional Ca 2+ channel or modulator properties and their regulation, a patch clamp technique to record membrane currents is required. This technique has wide applications ranging from recording the activity of native channels in their natural environment to that of recombinant channels expressed in heterologous cells. This chapter introduces the methods that have been used for the detection of calcium release-activated calcium (CRAC) currents, one of the store-operated calcium entry pathways, in human primary T cells. This standard protocol is for laboratories already equipped with a full patch clamp setup or for investigators collaborating with laboratories experienced in patch clamp.

DOI： 10.1007/978-1-4939-8802-0_18

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ejphar.2017.08.026

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jphs.2017.02.001

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1615/CritRevImmunol.2016017062

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   出版者・発行元：(公社)日本薬理学会  

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3892/ijmm.2012.1010

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担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/icb.2010.90

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ejphar.2005.10.067

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00011-005-1346-9

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記述言語：英語  

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記述言語：日本語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：WILEY-BLACKWELL  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：日本語   出版者・発行元：(公社)日本薬理学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPANESE PHARMACOLOGICAL SOC  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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