Updated on 2025/05/30

写真a

 
Kaneta Tsuyoshi
 
Organization
Graduate School of Science and Engineering (Science) Major of Science and Engineering Biology Senior Assistant Professor
Title
Senior Assistant Professor
Contact information
メールアドレス
Homepage
http://bio.sci.ehime-u.ac.jp/morphol/
External link

Degree

  • 修士(理学) ( 大阪大学 )

  • PhD. (science) ( Osaka University )

Research Interests

  • Cell elongation

  • Microtubules

  • plant hormones

  • gibberellin

  • Cytoskeleton

Research Areas

  • Life Science / Plant molecular biology and physiology

  • Life Science / Morphology and anatomical structure

  • Life Science / Cell biology  / Plant Cell Physiology

Education

  • 大阪大学大学院   理学研究科   博士後期課程 生理学専攻

    1994.4 - 1997.3

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  • Osaka University   Graduate School, Division of Natural Science   Department of Physiology

    - 1997

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  • 大阪大学大学院   理学研究科   博士前期課程 生理学専攻

    1992.4 - 1994.3

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    Country： Japan

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  • Osaka University   School of Science

    1988.4 - 1992.3

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    Country： Japan

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  • Osaka University   Faculty of Science   Department of Biology

    - 1992

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Research History

  • Ehime University

    2007.4

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  • Ehime University   Faculty of Science

    2005.4 - 2007.3

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  • Ehime University   Faculty of Science   Research Associate

    1999.8 - 2007.3

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  • - Faculty of Science, Ehime university

    1999

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  • 理化学研究所 国際フロンティア研究システム   ホルモン機能研究チーム   基礎科学特別研究員

    1997.4 - 1999.7

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  • Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN)

    1997 - 1999

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  • Osaka University   School of Science

    1996.4 - 1997.3

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  • Fuculty of Science, Osaka university

    1996 - 1997

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Professional Memberships

Papers

  • The Effect of Gibberellin and an Ethylene Inhibitor on Twining of Vine Cuttings in Japanese Morning Glory (<i>Ipomoea nil</i> (L.) Roth) Reviewed

    Tomoe Yofune, Nanami Matsumoto, Miyuki Funamoto, Tsuyoshi Kaneta

    The Horticulture Journal   93 ( 2 )   176 - 184   2024.4

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.qh-108

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  • Cell cycle-dependent dynamics of a plant intermediate filament motif protein with intracellular localization related to microtubules Reviewed

    Hikaru Utsunomiya, Masayuki Fujita, Fumio Naito, Tsuyoshi Kaneta

    Protoplasma   257 ( 5 )   1387 - 1400   2020.9

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00709-020-01512-1

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    Other Link： http://link.springer.com/article/10.1007/s00709-020-01512-1/fulltext.html

  • Programmed cell death of tobacco BY-2 cells induced by still culture conditions is affected by the age of the culture under agitation Reviewed

    Asahi Hiraga, Tsuyoshi Kaneta, Yasushi Sato, Seiichi Sato

    CELL BIOLOGY INTERNATIONAL   34 ( 2 )   189 - 196   2010.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    Evans Blue staining indicated that actively growing tobacco BY-2 cells in the exponential phase died more rapidly than quiescent cells in the stationary phase when the cells cultured under agitation were placed under still conditions. Fifty percent cell death was induced at about 18, 26, 80 and 140 h for early, mid, late exponential- and stationary-phase cells, respectively. Actively growing cells became TUNEL (transferase-mediated dUTP nick end labelling)-positive more rapidly than quiescent cells, suggesting that the cell death evaluated by Evans Blue is accompanied by DNA cleavages. Electrophoresis of genomic DNA showed a typical 'DNA laddering' pattern formed by multiples of about 200 bp internucleosomal units. Chromatin condensation was first detected at least within 24 h by light microscopy, and then cell shrinkage followed. These findings suggest that the death of BY-2 cells induced by still conditions is POD (programmed cell death).

    DOI： 10.1042/CBI20090003

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  • Analysis of expression profiles of three peroxidase genes associated with lignification in Arabidopsis thaliana Reviewed

    Naohito Tokunaga, Tsuyoshi Kaneta, Seiichi Sato, Yasushi Sato

    Physiologia Plantarum   136 ( 2 )   237 - 249   2009.6

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    Publishing type：Research paper (scientific journal)  

    We have investigated the mechanism of lignification during tracheary element (TE) differentiation using a Zinnia elegans xylogenic culture. In the process, we isolated ZPO-C, a peroxidase gene of Z. elegans that is expressed specifically in differentiating TEs. ZPO-C is suggested to be involved in lignification of Z. elegans TEs in vivo and in vitro. Furthermore, a peroxidase gene of Arabidopsis thaliana (AtPrx66), which is homologous to ZPO-C, was identified. The expression profile and functions of the gene in planta remain to be investigated. In this study, we performed promoter::β-glucuronidase (GUS) assays to investigate the expression profiles and functions of the ZPO-C-like peroxidases in A. thaliana. We generated transgenic A. thaliana lines carrying AtPrx66, AtPrx47 or AtPrx64 (peroxidases showing high sequence similarity to AtPrx66) promoter::GUS reporter gene fusions. The GUS activities of AtPrx66, AtPrx47 and AtPrx64 promoter::GUS lines were arranged concentrically from the center to the periphery in the roots of seedlings. Furthermore, histochemical GUS assays using inflorescence stems showed that AtPrx66, AtPrx47 and AtPrx64 promoter-driven GUS were mainly expressed in the differentiating vessels, xylem parenchyma and sclerenchyma, respectively. These results suggest that the gene expressions of these three peroxidases, which showed high sequence similarity to one another, are differentially regulated in various tissues and organs. In addition, our results suggest that while AtPrx66 and AtPrx47 are associated with lignification of vessels, AtPrx64 is associated with lignification of sclerenchyma. © Copyright Physiologia Plantarum 2009.

    DOI： 10.1111/j.1399-3054.2009.01233.x

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  • Heterologous expression of Na+/H+ antiporter gene (C nu NHA1) from salt-tolerant yeast Candida versatilis in Saccharomyces cerevisiae Na+-transporter deficient mutants Reviewed

    Yasuo Watanabe, Hidenori Akita, Yuka Higuchi, Rie Tsujimatsu, Tsuyoshi Kaneta, Youichi Tamai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 4 )   1005 - 1014   2008.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：TAYLOR & FRANCIS LTD  

    A Na+/H+ antiporter gene (C nu NHA1) was cloned from the salt-tolerant yeast Candida versatilis. C nu NHA1 encodes an antiporter with a typical yeast plasma membrane Na+/H+ antiporter structure. Transcription of C nu NHA1 in C. versatilis cells was dependent on the salinity of the culture. When C nu NHA1 was expressed in salt-sensitive Saccharomyces cerevisiae cells, increased salt-tolerance was observed, indicating that C nu nha1p possesses an Na+/H+ antiporter function, because the increased salt-tolerance was dependent on the extracellular pH. It appears that C nu nha1p mediates only the transport of Na+. In an S. cerevisiae transformant harboring a C nu NHA1-EGFP fusion plasmid in which the greater part of the C-terminal hydrophilic region of C nu nha1p was deleted by fusion with enhanced green fluorescent protein (EGFP), the C nu nha1-EGFP fusion protein was localized mainly in the plasma membrane, and the NaCl-tolerance of this transformant was greater than that of a strain harboring the entire C nu NHA1 gene.

    DOI： 10.1271/bbb.70752

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  • Comparative study of cellular structures implicated in gravisensing in statocytes of primary and lateral roots of Vigna angularis Reviewed

    N. Kuya, M. Kato, Y. Sato, T. Kaneta, S. Sato

    PROTOPLASMA   229 ( 1 )   83 - 91   2006.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER WIEN  

    The cellular structures of statocytes implicated in gravisensing in primary and lateral roots of Vigna angularis were compared. The statocytes of lateral roots already had small amyloplasts immediately after they emerged from the primary root. Although these amyloplasts sedimented, the lateral roots showed much weaker gravitropism than primary roots, at least until they reached a length of about 30 mm. The nuclei were usually positioned in the upper end of the statocytes in both types of roots. Electron microscopic surveys showed that many tubular elements of endoplasmic reticulum (ER) were frequently localized in the lower end of the statocyte and they sometimes diverged or curved, suggesting that the ER forms a large reticulate complex. It is worth noting that statocytes with a large ER complex were found much more frequently in primary roots than in lateral roots. The amyloplasts were not always settled on this complex but were very frequently under it, especially in the primary roots. In lateral roots, they were usually localized under the ER complex when they were present. Thus, it is suggested that the differential development and organization of the amyloplast-ER complex system is involved in the differential gravitropism of the two types of roots.

    DOI： 10.1007/s00709-006-0188-9

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  • Molecular cloning and gene expression of a fibrillarin homolog of tobacco BY-2 cells Reviewed

    Y. Makimoto, H. Yano, T. Kaneta, Y. Sato, S. Sato

    PROTOPLASMA   229 ( 1 )   53 - 62   2006.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER WIEN  

    Fibrillarin is known to play an important role in precursor ribosomal RNA processing and ribosome assembly. The present study describes a fibrillarin homolog gene isolated from tobacco BY-2 cells and its expression during the cell cycle. The cDNA for a fibrillarin homolog, named NtFib1, was first cloned in Nicotiana tabacum with degenerate primers. It encodes 314 amino acids and the deduced amino acid sequence has some highly conserved functional domains, such as the glycine and arginine-rich (GAR) domain for nucleolar localization and the RNA-binding motif. The C-terminal region is highly conserved and has 7 beta-sheets and 7 alpha-helices which are peculiar to fibrillarin. Thus, it is suggested that the fibrillarin homolog of this plant species functions in the same way as the fibrillarin already known from human and yeast cells. Northern blot analysis of BY-2 cells synchronized with aphidicolin or a combination of aphidicolin and propyzamide showed that the histone H4 gene was specifically expressed in the S phase but NtFib1 mRNA remained at high levels during the cell cycle. Examination of the localization of NtFib1 protein tagged with green-fluorescent protein (GFP) suggested that some persisting in the mitotic apparatus was eventually incorporated into reconstructed nucleoli in late telophase. Newly synthesized GFP-tagged NtFib1 protein in the cytoplasm was added to the recycled protein in early mitosis. Highly concentrated actinomycin D completely inhibited the transcription of genes coding for rRNA (rDNA) but did not significantly suppress the amount of either NtFib1 mRNA or protein, although the NtFib1 protein was reversibly dislocated from nucleoli. Although hypoxic shock completely prohibited rDNA transcription, NtFib1 mRNA remained at the same level as in the control experiment, even after the 4 h treatment. These results indicate that the transcription of NtFib1 mRNA is not related to rDNA transcription and NtFib1 mRNA is resistant to disrupting factors during the cell cycle.

    DOI： 10.1007/s00709-006-0183-1

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  • Nucleolonema as a fundamental substructure of the nucleolus Reviewed

    S Sato, H Yano, Y Makimoto, T Kaneta, Y Sato

    JOURNAL OF PLANT RESEARCH   118 ( 2 )   71 - 81   2005.4

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    Language：English   Publisher：SPRINGER TOKYO  

    The nucleolus is the most obvious structure in the eukaryotic nucleus. It is known to be a ribosome-producing apparatus where ribosomal (r) DNA is transcribed and the primary rRNA transcripts are processed to produce three of the four rRNA species. Electron microscopy has shown that the nucleolus consists of three major components, a dense fibrillar component (DFC), a granular component (GC) and a fibrillar center (FC). The DFC and FCs are integrated into a fundamental nucleolar substructure called the nucleolonema. The DFC corresponds to the matrix of the nucleolonema, and the FC is an electron microscopic counterpart of argyrophobic lacunae localized in the nucleolonema. The spherical FCs are intermittently arranged along the length of the nucleolonema in actively growing cells but are fused with each other to form tubular FCs when rDNA transcription is hampered. The RNase-gold complex does not bind to the FC but to the DFC and the GC, suggesting that rDNA transcription does not occur in the FC although both fluorescence in situ hybridization (FISH) and electron microscopic in situ hybridization reveal that the rDNA is specifically localized in the FCs. Immunogold-labeling after bromo-UTP (BrUTP) incorporation shows that rDNA transcription takes place in the boundary region between the FC and the DFC, and primary rRNA transcripts are expected to be processed outward within the DFC. Data have accumulated suggesting that the nucleolonema is a fundamental substructure of the nucleolus, and its skeleton is the tandem arrangement of the FCs, which are resting harbors or storages of rDNA. Ibis paper proposes that the transversal structural organization of the nucleolonema is centrifugally built up by several structural and functional domains: condensed and/or loosened rDNA, rDNA transcription zone, and transcript processing and ribosome assembly zones.

    DOI： 10.1007/s10265-005-0204-8

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  • Patterns of Dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development Reviewed

    T Montoya, T Nomura, T Yokota, K Farrar, K Harrison, JGD Jones, T Kaneta, Y Kamiya, M Szekeres, GJ Bishop

    PLANT JOURNAL   42 ( 2 )   262 - 269   2005.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：BLACKWELL PUBLISHING LTD  

    Brassinosteroids (BRs) are essential for many physiological functions in plants, however little is known concerning where and when they are synthesized. This is especially true during flower and fruit production, To address this we have used a promoter-GUS reporter fusion and RT-PCR to determine the relative expression levels of the tomato Dwarf (D) gene that encodes a BR C-6 oxidase. In young seedlings GUS reporter activity was observed mainly in apical and root tissues undergoing expansion. In flowers GUS activity was observed in the pedicel joints and ovaries, whereas in fruits it was strongest during early seed development and was associated with the locular jelly and seeds. RT-PCR analysis showed that tissue-specific expression of Dwarf mRNA was consistent with that of the Dwarf:GUS fusion. In good correlation with the high local Dwarf activity, quantitative measurements of endogenous BRs indicated intense biosynthesis in developing tomato fruits, which were also found to contain high amounts of brassinolide. Grafting experiments showed the lack of BR transport indicating that BR action occurs at the site of synthesis.

    DOI： 10.1111/j.1365-313X.2005.02376.x

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  • The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea Reviewed

    T Nomura, GJ Bishop, T Kaneta, JB Reid, J Chory, T Yokota

    PLANT JOURNAL   36 ( 3 )   291 - 300   2003.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

    Brassinosteroids (BRs) are growth-promoting plant steroid hormones, and in garden pea (Pisum sativum L.), the lka mutant is defective in BR perception. Here, we show that LKA encodes P. sativum BRI1 (PsBRI1), a homolog of BRI1, which is the Arabidopsis leucine-rich repeat receptor-like kinase/BR receptor. PsBRI1 was isolated by screening a pea cDNA library using Arabidopsis BRI1 cDNA as the probe. PsBRI1 is predicted to encode a 1188-amino-acid protein that has 78% similarity with Arabidopsis BRI1. Sequence analysis of PsBRI1 in the lka mutant led to the identification of a missense mutation that converts the highly conserved aspartic acid residue to asparagine, which is located in the leucine-rich repeat, just before the island domain that may bind BR or a BR-protein complex. The mutation identified in PsBRI1 co-segregated with the semi-erectoide lka phenotype. Transcript analysis of LKA/PsBRI1 indicates that it is ubiquitously expressed in pea and that the expression was downregulated by exogenous BR. The lka mutant was then utilized in further studies to analyze the independent actions of BR and gibberellin (GA) through the characterization of BR response on GA mutants and GA response on BR mutants.

    DOI： 10.1046/j.1365-313X.2003.01863.x

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  • Cloning the tomato curl3 gene highlights the putative dual role of the leucine-rich repeat receptor kinase tBRI1/SR160 in plant steroid hormone and peptide hormone signaling Reviewed

    T Montoya, T Nomura, K Farrar, T Kaneta, T Yokota, GJ Bishop

    PLANT CELL   14 ( 12 )   3163 - 3176   2002.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC PLANT BIOLOGISTS  

    Brassinosteroids (BRs) are plant steroid hormones that are essential for normal plant development. To gain better understanding of the conservation of BR signaling, the partially BR-insensitive tomato mutant altered brassinolide sensitivity1 (abs1) was identified and found to be a weak allele at the cur/3 (cu3) locus. BR content is increased in both of these mutants and is associated with increased expression of Dwarf. The tomato homolog of the Arabidopsis Brassinosteroid Insensitive1 Leu-rich repeat (LRR) receptor-like kinase, named tBri1, was isolated using degenerate primers. Sequence analysis of tBRI1 in the mutants cu3 and abs1 revealed that cu3 is a nonsense mutant and that abs1 is a missense mutant. A comparison of BRI1 homolog sequences highlights conserved features of BRI1 sequences, with the LRRs in close proximity to the island domain showing more conservation than N-terminal LRRs. The most homologous sequences were found in the kinase and transmembrane regions. tBRI1 (SR160) also has been isolated as the putative receptor for systemin, a plant peptide hormone. This finding suggests a possible dual role for tBRI1 in steroid hormone and peptide hormone signaling.

    DOI： 10.1105/tpc.006379

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  • Regulation of gibberellin biosynthesis genes during flower and early fruit development of tomato Reviewed

    M Rebers, T Kaneta, H Kawaide, S Yamaguchi, YY Yang, R Imai, H Sekimoto, Y Kamiya

    PLANT JOURNAL   17 ( 3 )   241 - 250   1999.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：BLACKWELL SCIENCE LTD  

    Gibberellins (GAs) are essential for the development of fertile flowers in tomato, and may also be required immediately after fertilization. In the GA-biosynthetic pathway, the reactions catalyzed by GA 20-oxidases have been implicated as site of regulation. To study the regulation of GA biosynthesis in flower and early fruit development, we isolated three tomato GA 20-oxidase cDNA clones, Le20ox-1 -2 and -3. The three genes showed different organ-specific patterns of mRNA accumulation. Analysis of the transcript levels of the three GA 20-oxidase genes, as well as those of copalyl diphosphate synthase (LeCPS) and GA 3 beta-hydroxylase (Le30H-2) during flower bud and early fruit development, revealed temporally distinct patterns of mRNA accumulation. Up until anthesis, transcripts were observed for LeCPS, Le20ox-1, -2 and Le30H-2, with an accumulation of Le20x-1 mRNA. In contrast to the high level of Le30H-2 transcripts in the fully open flower, mRNA levels of Le20x-1, -2 and LeCPS were reduced at this stage. After anthesis, LeCPS and Le20x-1 transcripts increased again. In addition, Le20ox-3 transcripts increased whereas the transcripts of Le30H-2 decreased to an un detectable level. In situ hybridization results demonstrated that during early stages of bud development, Le20x-2 transcripts were localized in the tapetum and placenta. The presented results supply novel data about localization of GA biosynthesis gene transcripts, and indicate that transcript levels of GA biosynthesis genes are all highly regulated during flower bud development.

    DOI： 10.1046/j.1365-313X.1999.00366.x

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  • S-Adenosyl-L-methionine : L-methionine S-methyltransferase from germinating barley Reviewed

    MJ Pimenta, T Kaneta, Y Larondelle, N Dohmae, Y Kamiya

    PLANT PHYSIOLOGY   118 ( 2 )   431 - 438   1998.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC PLANT PHYSIOLOGISTS  

    S-Adenosyl-L-methionine:L-methionine S-methyltransferase (MMT) catalyzes the synthesis of S-methyl-L-methionine (SMM) from L-methionine and S-adenosyl-L-methionine. SMM content increases during barley (Hordeum vulgare L.) germination. Elucidating the role of this compound is important from both a fundamental and a technological standpoint, because SMM is the precursor of dimethylsulfide, a biogenic source of atmospheric S and an undesired component in beer. We present a simple purification scheme for the MMT from barley consisting of 10% to 25% polyethylene glycol fractionation, anion-exchange chromatography on diethylaminoethyl-Sepharose, and affinity chromatography on adenosine-agarose. A final activity yield of 23% and a 2765-fold purification factor were obtained. After digestion of the protein with protease, the amino acid sequence of a major peptide was determined and used to produce a synthetic peptide. A polyclonal antibody was raised against this synthetic peptide conjugated to activated keyhole limpet hemocyanin. The antibody recognized the 115-kD denatured MMT protein and native MMT. During barley germination, both the specific activity and the amount of MMT protein increased. MMT-specific activity was found to be higher in the root and shoot than in the endosperm. MMT could be localized by an immunohistochemical approach in the shoot, scutellum, and aleurone cells but not in the root or endosperm (including aleurone).

    DOI： 10.1104/pp.118.2.431

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  • Gibberellin A(3) causes a decrease in the accumulation of mRNA for ACC oxidase and in the activity of the enzyme in azuki bean (Vigna angularis) epicotyls Reviewed

    T Kaneta, T Kakimoto, H Shibaoka

    PLANT AND CELL PHYSIOLOGY   38 ( 10 )   1135 - 1141   1997.10

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS  

    Differential screening, aimed at the isolation of cDNA clones of mRNAs whose accumulation is influenced by GA(3), resulted in the isolation of a cDNA clone of an mRNA whose level was decreased by GA(3) in segments of epicotyls of Vigna angularis. The putative protein encoded by this cDNA resembled the 1-aminocyclopropane-1-carboxylate oxidases (ACC oxidases) identified in other plant species (about 80% homology at the amino acid level). Thus, the corresponding gene was designated AB-ACO1 (azuki bean ACC oxidase). GA(3) also decreased the activity of ACC oxidase in azuki bean epicotyls, but it did not decrease the rate of ethylene evolution. In fact, GA(3) increased the rate of ethylene evolution and the level of ACC. Thus, GA(3) seemed to increase the production of ethylene by promoting the synthesis of ACC.

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  • ACTINOMYCIN-D INHIBITS THE GA(3)-INDUCED ELONGATION OF AZUKI BEAM EPICOTYLS AND THE REORIENTATION OF CORTICAL MICROTUBULES Reviewed

    T KANETA, T KAKIMOTO, H SHIBAOKA

    PLANT AND CELL PHYSIOLOGY   34 ( 7 )   1125 - 1132   1993.10

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS  

    Actinomycin D inhibited the elongation of epicotyl segments from azuki bean seedlings that was induced by simultaneous treatment with IAA and GA(3). The drug also inhibited the elongation of the segments that was caused by IAA alone when it was applied together with IAA. When the segments were pretreated with GA(3) and then incubated with IAA, GA(3) promoted the elongation caused by IAA and brought about a predominance of transverse cortical microtubules (MTs) in the epidermal cells of the segments. The change in the arrangement of MTs caused by pretreatment with GA(3) was evident 1 h after the start of subsequent incubation with IAA when the effect of pretreatment with GA(3) on the elongation had not yet become apparent. Pretreatment with GA(3) did not cause any change in the arrangement of MTs when GA(3)-pretreated segments were not incubated subsequently with IAA. Although actinomycin D applied before treatment with IAA did not inhibit the IAA-induced elongation, the drug diminished the promotion of the elongation caused by pretreatment with GA(3) and prevented GA(3) from bringing about a predominance of transverse MTs when the drug was applied during the pretreatment with GA(3).
    GA(3)-induced synthesis of mRNA seems to be involved in the promotion by GA(3) of IAA-induced elongation and in the GA(3)-induced rearrangement of cortical MTs.

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MISC

  • アサガオの切り出した蔓における巻き付きに対するジベレリンとエチレン阻害剤の効果 Reviewed

    夜舩 友咲, 松本 奈波, 船本 みゆき, 金田 剛史

    園芸学研究   23 ( 2 )   157 - 158   2024.4

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    Authorship：Last author   Language：Japanese   Publisher：園芸学会  

    Hort. J. 93 (2): 176-184の日本語の摘要
    蔓植物にとって、他のものへの “巻き付き” は生存する上で重要な形態形成反応である．蔓の巻き付き反応は支えとなるものとの接触刺激が引き金となると考えられており，“接触形態形成” の一つとみなすことができる．一般に，植物の接触形態形成の初期過程にはエチレンが重要な役割を果たしていると考えられているが，蔓の巻き付き反応とエチレンとの関連性については明らかにされていなかった．そこで本研究では，アサガオの植物体から切り出した蔓を用いて蔓の伸長と巻き付きに対するエチレン阻害剤の効果を調べた．まず，アサガオの切り出した蔓において巻き付き反応を再現するためにはジベレリンの添加が必要であることが判った．ジベレリン処理により伸長と巻き付きを誘導した切り出した蔓において，エチレン作用阻害剤である1-メチルシクロプロペンを処理したところ，支柱に巻き付いた角度が減少し，巻き付きが有意にゆるくなった．これらのことから，エチレンがアサガオの蔓において，支柱に沿って巻きつく際の接触の感知あるいは蔓の形態変化に関わっている可能性があることが示唆された．また，エチレン生合成経路における鍵酵素である1-アミノシクロプロパン-1-カルボン酸合成酵素 (ACS) のアサガオの遺伝子の中から，巻き付いている蔓において支柱に接している側で発現が高いものを，アサガオの蔓の巻き付きに関与する遺伝子の候補の一つとして見出した。

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  • 形質転換タバコBY‐2細胞におけるシロイヌナズナ中間径フィラメントモチーフタンパク質IFMoP1が形成する繊維構造の細胞周期依存的な変化及び微小管との局在関係

    内藤文雄, 藤田真幸, 久家徳之, 佐藤成一, 金田剛史

    日本植物生理学会年会要旨集   53rd   278   2012.3

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    Language：Japanese  

    J-GLOBAL

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  • 形質転換タバコBY‐2細胞の細胞質表層におけるシロイヌナズナ中間径フィラメントモチーフタンパク質IFMoP1の局在及びその経時的変化

    内藤文雄, 藤田真幸, 久家徳之, 佐藤成一, 金田剛史

    日本植物生理学会年会要旨集   52nd ( 0 )   295 - 726   2011.3

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    Language：Japanese   Publisher：The Japanese Society of Plant Physiologists  

    Intermediate filaments (IFs) are known as a kind of major cytoskeletons in animal cells. However, IFs in plant cells have not been identified yet. The deduced amino acid sequence of <I>Arabidopsis IFMoP1</I> gene has IF motifs and long &alpha;-helical structure which are conserved among animal IFs. Previously, we found that IFMoP1-GFP fusion protein, which was over-expressed in transgenic tobacco BY-2 cells, formed cytoskeleton like filamentous structures in cytoplasm around nuclei. We regard <I>IFMoP1</I> as a candidate for a gene encoding the IF protein in higher plants. <br> By fluorescence microscopy, it is revealed that structures which were formed from IFMoP1-GFP protein were fragmentated in the cell cortex and were arranged transversely to the cell axis. Additionally, time-lapse analysis showed that the filamentous structures moved randomly in cytoplasm around nuclei, and then were fragmentated. After the fragmentation of the structures, these were distributed and localized in cell cortex. The arrangement of fragments was similar to cortical microtubules arrays. It might co-localize with cortical microtubules.

    DOI： 10.14841/jspp.2011.0.0726.0

    J-GLOBAL

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  • アズキ側根におけるオーキシン排出担体VaPINの発現の組織特異性の解析

    久家徳之, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会大会研究発表記録   74th   226   2010.9

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    J-GLOBAL

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  • アズキ主根及び側根におけるオーキシン排出担体遺伝子VaPINのクローニングと発現解析

    久家徳之, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会大会研究発表記録   71st   131   2007.9

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    J-GLOBAL

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  • Cell death of tobacco BY-2 cells induced by static condition

    HIRAGA Asahi, SATO Yasushi, KANETA Tsuyoshi, SATO Seiichi

    119   139 - 139   2006.12

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    CiNii Books

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  • Cell death induced by bacterial infection in epicotyl of azuki beans seedlings

    ONO Masamichi, MATSUBA Mariko, SATO Yasushi, KANETA Tsuyoshi, SATO Seiichi

    119   139 - 139   2006.12

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    CiNii Books

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  • Comparative study of the magnitude of gravitropism and the features of statocytes in primary and lateral roots of Adzuki bean

    KUYA Noriyuki, SATO Yasushi, KANETA Tsuyoshi, SATO Seiichi

    J Plant Res   119 ( Supplement )   81 - 81   2006.12

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  • Patterns of Dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development (vol 42, pg 262, 2005)

    T Montoya, T Nomura, T Yokota, K Farrar, K Harrison, JGD Jones, T Kaneta, Y Kamiya, M Szekeres, GJ Bishop

    PLANT JOURNAL   42 ( 5 )   783 - 783   2005.6

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    Language：English   Publisher：BLACKWELL PUBLISHING LTD  

    DOI： 10.1111/j.1365-313X.2005.02445.x

    Web of Science

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  • CHARACTERIZATION OF THE GA UP-REGULATED GENE AtGAUR2 OF ARABIDOPSIS :

    KANETA Tsuyoshi, SHIRAI Miwa, KAKIMOTO Tatsuo

    Plant and cell physiology   42   s88   2001

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    Language：English   Publisher：Japanese Society of Plant Physiologists  

    CiNii Books

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    Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=184805

  • TISSUE SPECIFIC EXPRESSION OF GIBBERELLIN UP REGULATED GENES IN AZUKI BEAN EPICOTYLS

    KANETA Tsuyoshi, KAMIYA Yuji, SHIBAOKA Hiroh, KAKIMOTO Tatsuo

    39   S43 - S43   1998.5

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    CiNii Books

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  • Cloning of two cDNAs encoding gibberellin 3β-hydroxylase(Accession No. AB010991 and AB010992) of tomato seedlings(共著)

    American Society of Plant BiologistsPlant Physiology(The Electronic Plant Gene Register PGR98-200)   118   1534   1998

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  • ISOLATION OF GENES FOR mRNAs OF WHICH THE ACCUMULATIONS WERE INCREASED BY GA_3 FROM AZUKI BEAN EPICOTYLS

    KANETA Tsuyoshi, KAKIMOTO Tatsuo, SHIBAOKA Hiroh

    38   s114   1997.3

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    CiNii Books

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  • ISOLATION OF A GENE WHOSE EXPRESSION IS REDUCED BY GIBBERELLIN A3 FROM SEGMENTS OF AZUKI BEAN EPICOTYLS

    KANETA Tsuyoshi, KAKIMOTO Tatsuo, SHIBAOKA Hiroh

    37   141 - 141   1996.3

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    CiNii Books

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Presentations

  • 蔓が巻きつく際のオーキシンの役割及びDR5::GUS 形質転換アサガオを用いたオーキシン応答の可視化

    寳田悠, 夜舩友咲, 寺町香穂, 金田剛史

    第12 回アサガオ研究集会  2024.10 

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    Event date： 2024.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都府立大学 稲盛記念会館   Country：Japan  

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  • Visualization of auxin response in twining vines of morning glory

    Yu Takarada, Tomoe Yofune, Tsuyoshi Kaneta

    THE 80TH ANNUAL MEETING OF THE BOTANICAL SOCIETY OF CHUGOKU-SHIKOKU  2024.5  The Botanical Society of Chugoku-Shikoku

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    Event date： 2024.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

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  • ウツボカズラの捕虫器の起き上がり反応 ー 重力応答とアミロプラストの局在 ー

    寺町香穂, 金田剛史

    第64回日本植物生理学会年会（仙台）  2023.3 

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    Event date： 2023.3

    Language：Japanese   Presentation type：Poster presentation  

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  • 食虫植物ウツボカズラの捕虫器の起き上がりについて

    寺町香穂, 金田剛史

    日本植物学会第86回大会  2022.9 

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    Event date： 2022.9

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  • ウツボカズラの捕虫器の起き上がり反応 ― オーキシン輸送阻害剤の効果 ―

    寺町香穂, 金田剛史

    中国四国植物学会第78会大会  2022.5 

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    Event date： 2022.5

    Language：Japanese   Presentation type：Oral presentation (general)  

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    Tomoe Yofune, Kaho Teramachi, Tsuyoshi Kaneta

    The 85th Annual Meeting of the Botanical Society of Japan  2021.9 

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    Event date： 2021.9

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    Kaho Teramachi, Tsuyoshi Kaneta

    The 85th Annual Meeting of the Botanical Society of Japan  2021.9 

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    Event date： 2021.9

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  • Localization of endogenous auxin response in vine of transgenic morning glory which was introduced the auxin responsive promoter-GUS

    Tomoe Yofune, Kaho Teramachi, Tsuyoshi Kaneta

    The 77th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2021.6 

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    Event date： 2021.6

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Study on morphogenesis of the pitcher, an insect trap, of the Nepenthes: Improvement of the culture method for callus induction

    Kaho Teramachi, Tsuyoshi Kaneta

    The 77th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2021.6 

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    Event date： 2021.6

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  • The orientation of cortical microtubules in cells of a vine in morning glory －Involvement of cortical microtubules in vine twining－

    Kasumi Saito, Tomoe Yofune, Tsuyoshi Kaneta

    The 62st Annual Meeting of the Japanese Society of Plant Physiologists  2021.3 

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    Event date： 2021.3

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  • アサガオ形質転換体を用いたオーキシン応答性プロモーター GUS 解析

    夜舩 友咲, 寺町 香穂, 金田 剛史

    日本植物学会第84 回大会  2020.9 

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    Event date： 2020.9

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  • Expression of the ACC Synthase Gene in the Vine Tissue on the Side in the Touch with a Pole in Morning Glory

    Yofune, T, Kaneta, T

    The 61st Annual Meeting of the Japanese Society of Plant Physiologists  2020.3 

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    Event date： 2020.3

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  • The effect of gibberellin on colchicine inhibition of greening of callus in tissue culture of Arabidopsis

    The 51st annual meeting of JSPP  2010.3 

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  • アズキ上胚軸切片においてＧＡ３によって発現量の増加する遺伝子の単離

    金田剛史, 柿本辰男, 柴岡弘郎

    日本植物生理学会 １９９７年度年会  1997.3 

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  • Improvement of transformation via adventitious shoot induction from explants of azuki bean epicotyls.

    Myata R, Kaneta T

    The 67th Annual Meeting of the Chugoku-Shikoku Branch of the Botanical Society  2010.5 

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  • Five cDNA clones of mRNAs the amounts of which increased by GA<sub>3</sub> in azuki bean epicotyls. Invited International conference

    Tsuyoshi Kaneta, Tatsuo Kakimoto, Hiroh Shibaoka

    Frontier Research Forum, Frontiers of Gibberellin Research (part IV) , Wako-shi, RIKEN  1997.5 

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  • The diminishing effect of gibberellin on inhibition of greening of callus and disruption of microtubules by colchicine in Arabidopsis

    The 67th Annual Meeting of the Chugoku-Shikoku Branch of the Botanical Society  2010.5 

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  • アズキ側根におけるオーキシン排出担体 VaPIN の発現の組織特異性の解析

    久家徳之, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第74回大会(愛知)  2010.9 

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  • ジベレリンによるアズキ上胚軸の伸長成長促進に関連した細胞質表層微小管の配向変化とアクチンフィラメント

    井上 瞳, 金田剛史

    第６８回日本植物学会中国四国支部大会（香川大会）  2011.5 

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  • シロイヌナズナのカルスにおけるタキソールの低濃度処理による成長抑制と高濃度処理による緑化回復

    近藤 万季, 金田剛史

    日本植物学会第75回大会（東京）  2011.9 

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  • Up-regulated expression of FT genes during bulbing in onion (Allium cepa).

    Tagashira M, Kaneta T

    The 70th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2013.5 

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  • Identification of bulbing hormone genes in onion (Allium cepa).

    Minami Tagashira, Tsuyoshi Kaneta

    The 55th annual meeting of JSPP  2014.3 

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  • The effect of actin filament disruption on gibberellin-induced reorientation of cortical microtubules in epidermal cells of azuki bean epicotyls

    Inoue H, Kaneta T

    The 69th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2012.5 

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  • タマネギにおける3種のFT遺伝子のクローニング及び日長による発現制御の違い

    田頭みなみ, 金田剛史

    中国四国植物学会 第６９回大会（島根）  2012.5 

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    この発表を行った学生（４回生）田頭みなみが「優秀発表賞（ポスター発表部門）」を受賞

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  • タバコBY-2培養細胞の静置培養により誘導される細胞死におよぼす細胞周期の影響

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第76回大会(姫路)  2012.9 

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  • Localization of Cortical Microtubules and Actin Filaments and Expression of Homologous Genes for Kinesin with a Calponin Homology Domain in Elongating Epicotyls of Azuki Bean during Auxin and Gibberellin Treatment

    2013.3 

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  • アズキ「エリモショウズ」におけるカルス化を介した高率のシュート誘導法 ～形質転換体作製への応用をめざして～

    久米 佐和, 金田剛史

    日本植物学会第75回大会（東京）  2011.9 

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  • 植物細胞における中間径フィラメントモチーフタンパク質に関する研究

    金田 剛史

    第３回愛媛大学学術フォーラム  2012.1 

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    ポスター発表（萌芽研究成果発表）

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  • Cell cycle dependent dynamics of filament composed of Arabidopsis Intermediate Filament Motif Protein 1 (IFMoP1) and distribution of microtubules in transgenic tobacco BY-2 cells

    The 53rd annual meeting of JSPP  2012.3 

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  • Cell cycle dependent changes in structure composed of Arabidopsis intermediate filament motif protein 1 (IFMoP1) and its localization in transgenic tobacco BY-2 cells

    Naito F, Fujita M, Sato S, Kaneta T

    The 69th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2012.5 

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  • Auxin up-regulates expression of the gene for kinesin with a calponin-homology domain.

    Inoue H, Kaneta T

    The 70th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2013.5 

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  • アズキ主根及び側根におけるオーキシン排出担体遺伝子VaPINのクローニングと発現解析

    久家徳之, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第７１回大会、野田  2007.9 

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  • タバコBY-2細胞において発現させたGFP融合タンパク質を利用したシロイヌナズナ中間径フィラメントモチーフタンパク質の局在解析

    藤田真幸, 佐藤成一, 金田剛史

    日本植物学会中国四国支部第６５回大会、広島  2008.5 

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  • ジベレリンの茎の伸長促進作用における転写の関与

    金田剛史, 柿本辰男, 柴岡弘郎

    日本植物生理学会 １９９３年度年会、金沢  1993.3 

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  • Involvement of GA<sub>3</sub>-induced gene expression in GA<sub>3</sub> promotion of stem elongation International conference

    Tsuyoshi Kaneta, Tatsuo Kakimoto, Hiroh Shibaoka

    XV International Botanical Congress, Yokohama  1993.8 

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  • 正重力屈性におけるアミロプラストの発達及び微小管配列の関係

    春田剛史, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第７２回大会、高知  2008.9 

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  • シロイヌナズナの中間径フィラメントモチーフタンパク質の細胞内局在及び遺伝子発現の組織特異性

    藤田真幸, 佐藤成一, 金田剛史

    第５０回日本植物生理学会年会（名古屋）  2009.3 

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  • High efficiency of adventitious shoot induction from epicotyl segments of azuki bean seedlings.

    The 66th Annual Meeting of the Chugoku-Shikoku Branch of the Botanical Society  2009.5 

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  • Gibberellin stabilizes microtubules against microtubule-disrupting effect of colchicine in cells of Arabidopsis callus

    The 66th Annual Meeting of the Chugoku-Shikoku Branch of the Botanical Society  2009.5 

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  • Cyclosporin A が静置培養により誘導されるタバコBY-2細胞のプログラム細胞死に与える影響

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中国四国支部第６５回大会、広島  2008.5 

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  • 塩ストレスによるタバコBY-2細胞の細胞死

    子安舞衣, 戸田知沙, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中国四国支部第６５回大会、広島  2008.5 

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  • 塩ストレスにより誘導されるタバコ培養細胞の細胞死について

    戸田知沙, 子安舞衣, 金田剛史, 佐藤康, 佐藤成一

    日本植物形態学会第２０回総会・大会、高知  2008.9 

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  • 培地や増殖期がタバコBY-2 細胞の静置培養における細胞死の誘導に与える影響

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中国四国支部第66回大会高知大会  2009.5 

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  • タバコBY-2細胞の静置培養による細胞死誘導とその要因について

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第73回大会（山形）  2009.9 

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  • High efficiency of adventitious shoot induction from epicotyl explants of azuki bean cv. Erimo-shouzu seedlings

    Miyata R, Kaneta T

    The 51st annual meeting of JSPP  2010.3 

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  • Gibberellin up regulated genes (GAUR1-5) in azuki bean epicotyls. International conference

    金田剛史, 神谷勇治, 柴岡弘郎, 柿本辰男

    第１６回国際植物生長物質会議 幕張メッセ  1998.8 

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  • 光発芽レタス種子におけるジベレリン生合成酵素遺伝子の発現の局在

    中南健太郎, 豊増友伸, 三橋渉, 倉橋敏裕, 金田剛史, 井上康則, 神谷勇治

    日本植物生理学会 １９９９年度年会  1999.3 

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  • レタス種子の発芽に関与するジベレリン生合成酵素遺伝子の発現の光制御とその局在

    豊増知伸, 川出洋, 金田剛史, 三橋渉, 井上康則, 神谷勇治

    日本植物生理学会 １９９９年度年会・第３９回シンポジウム  1999.3 

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  • ジベレリンによって発現が誘導されるAtGAUR2遺伝子の解析

    金田剛史, 白井美和, 柿本辰男

    日本植物生理学会２００１年度年会  2001.3 

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  • アズキ上胚軸切片においてＧＡ<sub>３</sub>によって発現量の増加する遺伝子の発現の組織特異性

    金田剛史, 神谷勇治, 柴岡弘郎, 柿本辰男

    日本植物生理学会 １９９８年度年会、札幌  1998.5 

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  • アズキ主根及び側根における重力屈性と平衡細胞の関係

    久家徳之, 佐藤康, 金田剛史, 佐藤成一

    日本植物学会第７０回大会、熊本  2006.9 

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  • タバコ培養細胞におけるフィブリラリン遺伝子のクローニングとその発現

    槙本祐治, 金田剛史, 佐藤成一

    日本植物学会第６７回大会、札幌  2003.9 

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  • 低酸素で誘導される根における細胞死の特徴

    巻田優花, 金田剛史, 佐藤成一

    日本植物学会第６９回大会、富山  2005.9 

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  • ジベレリンによるアズキ上胚軸の伸長促進におけるアクチンフィラメントの関与

    金田剛史, 松本裕子, 佐木宣親

    日本植物学会中四国支部大会愛媛大会  2006.5 

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    Language：Japanese   Presentation type：Poster presentation  

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  • 静置状態により誘導されるタバコ培養細胞BY-2のプログラム細胞死

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中国四国支部第６３回大会、愛媛  2006.5 

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  • How is the stem twining controlled in morning glory ? ―Expression analysis of ACC synthase genes and examination of auxin-responsive promoter assay―

    Yofune T, Funamoto M, Kaneta T

    The 59th annual meeting of JSPP  2018.3 

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  • Plant hormonal regulation of vine twining in morning glory ―Study on involvement of ethylene and auxin―

    Yofune T, Funamoto M, Kaneta T

    The 75th Annual Meeting of The Botanical Society of Chugoku-Shikoku  2018.5 

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  • Role of ethylene on vine twining in morning glory.

    Yofune T, KANETA T

    The 82th Annual Meeting of the Botanical Society of Japan  2018.9 

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  • Changes in localization of plant intermediate filament motif protein in cell cycle-synchronized tobacco BY-2 cells

    Utsunomiya H, Fujita M, Kaneta T

    The 58th annual meeting of JSPP  2017.3 

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    Venue：鹿児島市  

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  • Localization of intermediate filament motif protein changes depending on the cell cycle in plant cells.

    Utsunomiya H, Kaneta T

    The 74th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2017.5 

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  • Expression analysis of jasmonic acid biosynthetic genes in vine of Japanese morning glory (Ipomoea nil).

    Yofune T, Kaneta T

    The 74th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2017.5 

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  • アサガオの蔓の巻き付きに表層微小管は関与するのか？

    夜舩友咲, 松本奈波, 蘭理恵子, 庄田彩乃, 金田剛史

    日本植物学会第81回大会  2017.9 

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    Venue：野田市  

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  • Involvement of cortical microtubules in twining stems of the morning glory (Ipomoea nil).

    Matsumoto N, Kaneta T

    The 71st Annual Meeting of the Botanical Society of Chugoku-Shikoku  2014.5 

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  • Involvement of the orientation of cortical microtubules in helical elongation of stem in Juncus effusus var. spiralis.

    Umakoshi M, Nomura C, Saho M, Fujimoto S, Kaneta T

    The 71st Annual Meeting of the Botanical Society of Chugoku-Shikoku  2014.5 

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  • Is jasmonic acid involved in twining of vine in Japanese morning glory (Ipomoea nil)?

    Yofune T, Shoda A, Araragi R, Kaneta T

    The 73rd Annual Meeting of the Botanical Society of Chugoku-Shikoku  2016.5 

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    Venue：米子市  

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  • Cell cycle synchronization reveals changes in structure composed of Arabidopsis intermediate filament motif protein in transgenic tobacco BY-2 cells.

    Utsunomiya H, Kaneta T

    The 73rd Annual Meeting of the Botanical Society of Chugoku-Shikoku  2016.5 

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    Venue：米子市  

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  • アサガオの蔓の巻き付きとエチレンとの関連性の検討—ACC合成酵素遺伝子の発現解析—

    夜舩友咲, 金田剛史

    第10回アサガオ研究集会  2019.3 

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    Venue：茨城大学  

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  • Is Ethylene Involved in the Twining of the Morning Glory? - Expression Analysis of ACC Synthase Genes in the Stem - International conference

    Tomoe Yofune, Tsuyoshi Kaneta

    The 60th Annual Meeting of the Japanese Society of Plant Physiologists and Japan-Taiwan Plant Biology 2019 (JTPB2019),  2019.3 

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    Venue：Nagoya  

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  • Cell Cycle Dependent Changes in Localization of Arabidopsis Intermediate Filament Motif Protein in Transgenic Tobacco BY-2 Cells and the Expression of a Homologous Gene.

    Hitomi Yamashita, Hikaru Utsunomiya, Tsuyoshi Kaneta

    The 60th Annual Meeting of the Japanese Society of Plant Physiologists  2019.3 

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    Venue：Nagoya  

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  • The key enzyme genes for ethylene biosynthesis whose expression is related to twining of vines in Japanese morning glory

    Tomoe Yofune, Tsuyoshi Kaneta

    The 76th Annual Meeting of the Botanical Society of Chugoku-Shikoku  2019.5 

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    Venue：Hiroshima  

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  • ジベレリンによるアズキ上胚軸の伸長成長時の細胞質表層微小管とアクチンフィラメントの局在

    井上 瞳, 金田剛史

    日本植物学会第75回大会（東京）  2011.9 

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  • タバコBY-2 細胞の細胞周期と静置培養により誘導される細胞死との関係

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第74回大会（愛知）  2010.9 

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  • Localization of Arabidopsis Intermediate Filament Motif Protein 1 (IFMoP1) in cell cortex of transgenic tobacco BY-2 cells and its time dependent changes

    Naito F, Fujita M, Kuya N, Sato S, Kaneta T

    The 52nd annual meeting of JSPP  2011.3 

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  • 形質転換タバコBY-2細胞においてシロイヌナズナ中間径フィラメントモチーフタンパク質IFMoP1が形成する繊維の核周辺における複雑な挙動とその局在変化

    内藤文雄, 藤田真幸, 久家徳之, 佐藤成一, 金田剛史

    第６８回日本植物学会中国四国支部大会（香川大会）  2011.5 

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  • アズキ上胚軸外植片を用いたカルス化を介する組織培養系によるシュート

    久米 佐和, 宮田 麗香, 金田剛史

    第６８回日本植物学会中国四国支部大会（香川大会）  2011.5 

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  • アズキ発芽種子の上胚軸に現れる病変とその原因

    小野真道, 佐藤康, 金田剛史, 佐藤成一

    日本植物学会第７１回大会、野田  2007.9 

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  • 静置状態により誘導されるタバコ培養細胞BY-2の細胞死の形態学的特徴

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中四国支部大会鳥取大会  2007.5 

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  • アズキのオーキシン排出担体遺伝子のクローニングと主根と側根における発現

    久家徳之, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中四国支部大会鳥取大会  2007.5 

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  • 核小体タンパク質フィブリラリンの分裂期における挙動

    佐藤奈苗, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会中四国支部大会鳥取大会  2007.5 

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  • 静置培養により誘導されるタバコ培養細胞BY-2の細胞死

    平賀旭, 佐藤康, 金田剛史, 佐藤成一

    日本植物学会第７０回大会、熊本  2006.9 

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  • アズキ発芽種子の上胚軸において細菌感染が誘導する細胞死

    小野真道, 松葉麻梨子, 佐藤康, 金田剛史, 佐藤成一

    日本植物学会第７０回大会、熊本  2006.9 

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  • 静置条件下により誘導されるタバコBY-2細胞の細胞死と細胞構造

    平賀旭, 金田剛史, 佐藤康, 佐藤成一

    日本植物学会第７１回大会、野田  2007.9 

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  • アズキ発芽種子上胚軸に見られる病変の特徴及びその原因

    小野真道, 佐藤康, 金田剛史, 佐藤成一

    日本植物学会中四国支部大会鳥取大会  2007.5 

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  • アズキ上胚軸切片においてジベレリンにより発現量の変化する遺伝子の解析

    金田剛史, 柿本辰男, 柴岡弘郎

    日本植物生理学会 １９９６年度年会  1996.3 

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Research Projects

  • 蔓植物の形態に関する生理学的および分子生物学的な研究

    2021.4 - 2026.3

    日本学術振興会  科研費  基盤研究(C)

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    Authorship：Principal investigator 

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  • 植物細胞における中間径フィラメントの働きの解明

    2016.4 - 2020.3

    日本学術振興会  科研費 挑戦的萌芽研究 

    金田剛史

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）

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  • ジベレリンによる植物細胞の伸長促進の作用機構

    1996.4 - 1997.3

    日本学術振興会  特別研究員奨励費 

    金田剛史

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    Authorship：Principal investigator  Grant type：Competitive

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Other

  • 「中国四国植物学会第78会大会」における指導大学院生の「優秀発表賞（口頭発表部門）」受賞

    2022.5

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    令和4年5月21日(土)，22日(日)，オンライン（主催: 島根大学）で開催された「中国四国植物学会第78回大会」において，当研究室の大学院生1名が「優秀発表賞（口頭発表部門）」を受賞した。
    テーマ： 「ウツボカズラの捕虫器の起き上がり反応 ― オーキシン輸送阻害剤の効果 ―」
    演者： 理工学研究科博士後期課程1年 寺町香穂

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  • 「中国四国植物学会第76会大会」における指導大学院生の「優秀発表賞（口頭発表部門）」受賞

    2019.5

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    令和元年5月11日(土)，12日(日)，広島大学で開催された「中国四国植物学会第76回大会」において，当研究室の大学院生1名が「優秀発表賞（口頭発表部門）」を受賞した。
    テーマ： 「アサガオにおいて蔓の巻き付きに伴い発現が変化するエチレン生合成鍵酵素の遺伝子」
    演者： 理工学研究科博士後期課程1年 夜舩友咲

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  • 中国四国植物学会第74回大会」における指導大学院生の優秀発表賞(口頭発表部門)受賞

    2017.5

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    平成29年5月13日(土)，14日(日)，高知大学で開催された「中国四国植物学会第74回大会」において，当研究室の大学院生1名が「優秀発表賞(口頭発表部門)」を受賞した。
    発表テーマ：「植物細胞において中間径フィラメントモチーフタンパク質の局在は細胞周期依存的に変化する」
    演者：宇都宮輝

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  • 中国四国植物学会第74回大会」における指導大学院生の「優秀発表賞(ポスター発表部門)」受賞

    2017.5

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    平成29年5月13日(土)，14日(日)，高知大学で開催された「中国四国植物学会第74回大会」において，当研究室の大学院生1名が「優秀発表賞(ポスター発表部門)」を受賞した。
    発表テーマ：「アサガオの蔓におけるジャスモン酸生合成関連酵素の遺伝子の発現解析」
    演者：夜舩友咲

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  • 中国四国植物学会第73回大会における指導学生の優秀発表賞(ポスター発表部門)」受賞

    2016.5

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    平成28年5月14日(土)，15日(日)，米子市で開催された「中国四国植物学会第73回大会」において，当研究室の卒論生が「優秀発表賞(ポスター発表部門)」を受賞した。
    発表テーマ：「アサガオの巻き付きにジャスモン酸は関与するのか」
    演者：夜舩友咲

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  • 中国四国植物学会第71回大会における平成25年度高大連携授業「附属高校課題研究」受講生の優秀発表賞(ポスター発表部門)受賞

    2014.5

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    平成26年5月10日(土)，11日(日)，岡山理科大学で開催された「中国四国植物学会第71回大会」において当研究室で附属高校課題研究を行った学生が優秀発表賞（ポスター発表部門）を受賞した。
    発表テーマ：「ラセンイの茎のらせん伸長と微小管の配向との関係」
    演者：馬越真由佳

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  • 中国四国植物学会第71回大会における指導学生の優秀発表賞（口頭発表部門）受賞

    2014.5

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    平成26年5月10日(土)，11日(日)，岡山理科大学で開催された「中国四国植物学会第71回大会」において当研究室の4回生のが優秀発表賞（口頭発表部門）を受賞した。
    発表テーマ：「アサガオの支柱への巻き付きにおける表層微小管の関与」
    演者：松本奈波

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  • 第52 回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会（平成25年10月，松山）の中の「高校生オープン学会」における附属高校課題研究指導学生の優秀発表賞（ポスター部門）受賞

    2013.10

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    第52 回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会（平成25年10月，松山）の中の「高校生オープン学会」において，当研究室で高大連携授業「附属高校課題研究」で指導を受けた愛媛大学附属高校の生徒の研究発表が優秀発表賞（ポスター部門）を受賞した。
    研究テーマ：「ラセンイの茎はなぜらせん状に伸びるのか」
    演者：馬越真由佳

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  • 中国四国植物学会第70回大会における指導学生の優秀発表賞（口頭発表部門）

    2013.5

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    当研究グループの理工学研究科博士前期課程1年の大学院生1名が，平成25年5月11日（土)～12日（日)に徳島大学で開催された中国四国植物学会第70回大会において，学会会員の投票による優秀発表賞（口頭発表部門)を受賞した。
    研究テーマ：「タマネギの鱗茎形成期におけるFT遺伝子の発現量の増加 」
    演者：田頭みなみ

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  • 中国四国植物学会第69回大会における指導学生の優秀発表賞（ポスター発表部門）受賞

    2012.5

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    当研究グループの卒業研究生1名が，平成24年5月12日（土)～13日（日)に島根大学で開催された中国四国植物学会第69回大会において，それぞれ優秀発表賞（ポスター発表部門）を受賞した。この賞は，中国四国植物学会において，主として本人が行った研究成果についてポスターまたは口頭発表を行った学生あるいは若手研究者（40歳未満の研究員や助教）の中から選出され，優秀発表賞（ポスター発表部門，口頭発表部門）として表彰されるもので，今回の大会から新たに創設されたもの。
    研究テーマ：「タマネギにおける3種のFT遺伝子のクローニング及び日長による発現制御の違い」
    演者：田頭みなみ

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  • 中国四国植物学会第69回大会にける指導学生の優秀発表賞（口頭発表部門)受賞

    2012.5

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    当研究グループの理工学研究科博士前期課程1年の大学院生1名が，平成24年5月12日（土)～13日（日)に島根大学で開催された中国四国植物学会第69回大会において，優秀発表賞（口頭発表部門)を受賞した。これらの賞は，中国四国植物学会において，主として本人が行った研究成果についてポスターまたは口頭発表を行った学生あるいは若手研究者（40歳未満の研究員や助教）の中から選出され，優秀発表賞（ポスター発表部門，口頭発表部門）として表彰されるもので，今回の大会から新たに創設されたもの。
    発表テーマ：「アズキ上胚軸におけるジベレリンによる細胞質表層微小管の配向変化に対してアクチンフィラメントの破壊が及ぼす影響」
    演者：井上瞳

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  • 外国人研究者訪問

    2008.3

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    Maria Joao Pimenta Lange （ドイツ）

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  • 外国人研究者訪問

    2008.3

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    Theo Lange（ドイツ）

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Teaching Experience (On-campus)

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Teaching Experience

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Social Activities

  • 第20回 愛媛大学理学部サマースクール 親子で楽しむ科学実験

    Role(s)： Organizing member

    2019.8

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    Audience： Schoolchildren,　Guardians

    テーマ「葉脈でしおりを作って観察しよう」の実験準備・実施全般を担当。2日間。

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  • 平成29年度教員免許状更新講習の講師

    Role(s)： Lecturer

    愛媛大学  2017.11

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    Audience： Teachers

    Type：Research consultation

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  • 平成２９年度サイエンスプリンセスプロジェクト「ひめこと回る研究室見学会」への協力

    Role(s)： Organizing member

    愛媛大学ダイバーシティ推進本部 女性未来育成センター主催  2017.8

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    Audience： Junior students,　High school students

    Type：Other

    理系女子による研究室紹介ツアー。担当学生が「ひめこ」として参加し、当研究室の紹介も共に行った。

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  • 松山赤十字看護専門学校「生命科学」講義担当

    Role(s)： Lecturer

    松山赤十字看護専門学校  2016.6 - 2016.7

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    Type：Other

    １５回の授業の担当

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  • 松山赤十字看護専門学校「生命科学」講義担当

    Role(s)： Lecturer

    松山赤十字看護専門学校  2015.9 - 2015.12

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    Type：Other

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  • 松山赤十字看護専門学校「生命科学」講義担当

    Role(s)： Lecturer

    松山赤十字看護専門学校  2014.9 - 2014.12

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    Type：Other

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  • サイエンスプリンセスプロジェクト２０１３「ひめこと回る理系いりいろ見学ツアー」への協力

    Role(s)： Organizing member

    愛媛大学ダイバーシティ推進本部 女性未来育成センター主催  2013.8

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    Type：Other

    理系女子による研究室紹介ツアー。担当学生が「ひめこ」として参加し、当研究室の紹介も共に行った。

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  • 附属高校課題研究の指導

    Role(s)： Advisor

    愛媛大学附属高校  2013.4 - 2013.9

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    Type：Research consultation

    高大連携事業。担当生徒は１名。

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  • 第１３回愛媛大学理学部サマースクール「親子で楽しむ科学実験−君もミニ博士になれる！−」

    Role(s)： Planner,　Organizing member

    愛媛大学理学部  2011.8

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    Type：Other

    委員長

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  • 平成23年度教員免許状更新講習の講師

    Role(s)： Lecturer

    愛媛大学  2011.8

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    Type：Certification seminar

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  • 女子高校生の理工系チャレンジ支援事業

    Role(s)： Organizing member

    愛媛大学  2010.8

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    Type：Other

    研究室訪問等

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  • 愛媛県総合教育センター主催の「理数系教員指導力向上研修（探究心を高める高校理科教員ステップアップ研修）」の講師

    Role(s)： Lecturer

    愛媛県総合教育センター  2009.6

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    Audience： Teachers

    Type：Other

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  • 松山北高等学校2年生を対象とした出張講義「植物の形の調節」の講師

    Role(s)： Lecturer

    2009.6

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    Audience： High school students

    Type：Visiting lecture

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  • 愛媛大学理学部サマースクール「親子で楽しむ科学実験−君もミニ博士になれる！−」

    Role(s)： Organizing member

    愛媛大学理学部  2008.8

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    Audience： Schoolchildren,　Guardians

    Type：Other

    テーマ「葉脈でしおりをつくろう」の実験準備・実施全般を担当。2日間。

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  • 第４８回日本植物生理学会年会

    Role(s)： Organizing member

    日本植物生理学会  愛媛大学  2007.3

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    Audience： Researchesrs

    Type：Other

    総務・会場係

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  • 愛媛大学理学部サマースクール「親子で楽しむ科学実験−君も豆博士になれる！−」

    Role(s)： Organizing member

    愛媛大学理学部  2006.8

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    Audience： Schoolchildren,　Guardians

    Type：Other

    「葉脈でしおりをつくろう」実験補助および指導を担当

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  • 県立松山南高等学校スーパーサイエンスハイスクール事業「研究室体験」

    Role(s)： Lecturer

    2005.12

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    Audience： High school students

    Type：Other

    愛媛大学研究室体験・理学部・生物学科・植物形態学研究室。2005年12月14日午後と12月21日午後の計6時間にわたって松山南高等学校の生徒3名の「研究室体験」を指導した。

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  • 平成28年度 愛媛大学研究室体験（松山南高等学校スーパーサイエンスハイスクール事業支援）

    Role(s)： Lecturer,　Organizing member

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    Audience： High school students

    Type：Other

    松山南高等学校生を対象に以下の活動を行った。
    （１）研究室見学
    ＊ 実験室・植物培養室・学生部屋の見学。
    ＊ 研究内容の紹介：
    「タマネギの肥大の仕組みについて」
    「アサガオの蔓の巻き付きの仕組みについて」
    （２）実験体験
    ＊ アズキの茎切片を用いた植物ホルモンの作用を調べる実験。
    ＊ 植物細胞の細胞骨格の観察。

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Media Coverage

  • 「News Ch.4」において指導学生が愛媛大で活躍する女性研究者として紹介される件での取材対応。 TV or radio program

    南海放送  愛媛大学理学部  2014.2

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    「News Ch.4」において、指導学生の一人が愛媛大で活躍する女性研究者として紹介される件についての取材対応。

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  • 指導している大学院生の「いよ×イチ(ニュース番組）」出演および研究室撮影への協力 TV or radio program

    NHK  愛媛大学理学部植物形態学研究室  2013.6

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    指導している大学院生の「いよ×イチ(ニュース番組）」出演および研究室撮影への協力。

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  • 学生の学会賞受賞および研究内容についての取材対応 Newspaper, magazine

    愛媛新聞  愛媛大学理学部  2013.6

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    学生の学会賞受賞および研究内容についての取材対応。内容は、愛媛新聞に掲載。

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  • 親子で楽しむ科学実験の取材対応 Newspaper, magazine

    愛媛新聞  愛媛大学理学部  2011.8

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    親子で楽しむ科学実験の取材対応。記事は２２日朝刊に掲載。

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  • ＳＳＨ松山南高等学校活動協力

    愛媛大学理学部  2011.8

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    第１３回愛媛大学理学部サマースクール「親子で楽しむ科学実験−君もミニ博士になれる！−」において、松山南高の教員と生徒（生物部、化学部）による２テーマ担当の仲介。

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  • 親子で楽しむ科学実験の取材対応 TV or radio program

    南海放送  愛媛大学理学部  2011.8

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    親子で楽しむ科学実験の取材対応。テレビニュースで紹介された。

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  • 親子で楽しむ科学実験の取材対応 Newspaper, magazine

    読売新聞  愛媛大学理学部  2011.8

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    親子で楽しむ科学実験の取材対応。記事は２１日の朝刊に掲載。

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  • 「親子で楽しむ科学実験」の取材協力 Newspaper, magazine

    朝日新聞  愛媛大学理学部  2008.8

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    「親子で楽しむ科学実験」実施中の取材及び写真撮影に応じた。実験中の親子の写真が地方欄に掲載された。

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