記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Spandidos Publications  

Human papillomavirus (HPV) is a possible carcinogenetic factor in oral squamous cell carcinoma (OSCC). Previous studies have reported the prevalence of HPV in patients with OSCC. However, the association between HPV and OSCC remains controversial. The present study aimed to clarify the association between HPV infection, p16 protein expression and the clinicopathological characteristics of OSCC. The expression level of HPV-16E6 mRNA and p16 protein, a known surrogate marker of HPV infection, was investigated in 100 OSCC cases using TaqMan reverse transcription-quantitative PCR and immunohistochemistry staining, respectively. HPV-16E6 mRNA expression level was only detected in one case (1%), and positive expression of p16 was found in 10 cases (10%), including an HPV-positive case. Subsequently, the association between p16 expression level and clinicopathological characteristic factors were analyzed; however, no significant association was found. These results suggested that HPV-16 infection was less likely to cause OSCC in Japan and p16 expression was not a suitable marker for HPV infection in OSCC.

DOI： 10.3892/ol.2021.12789

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記述言語：日本語   出版者・発行元：愛媛医学会  

薬剤関連顎骨壊死(MRONJ)は、ビスフォスフォネート製剤や抗RANKL抗体などの骨吸収抑制薬投与により、一定の確率で起こりうる有害事象である。当初MRONJは、保存療法が中心の難治性疾患であったが、最近では病態の理解も進み、手術療法や薬剤により寛解する症例も増加し、その対策も変化している。本トピックスでは、MRONJの現状と対策につき、当科の症例も交えて解説する。(著者抄録)

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Molecules that demonstrate a clear association with the aggressiveness of oral squamous cell carcinoma (OSCC) have not yet been identified. The current study hypothesized that tumor cells in OSCC have three different origins: Epithelial stem cells, oral tissue stem cells from the salivary gland and bone marrow (BM) stem cells. It was also hypothesized that carcinomas derived from less-differentiated stem cells have a greater malignancy. In the present study, sex chromosome analysis by fluorescence in situ hybridization and/or microdissection PCR was performed in patients with OSCC that developed after hematopoietic stem cell transplantation (HSCT) from the opposite sex. OSCC from 3 male patients among the 6 total transplanted patients were considered to originate from donor-derived BM cells. A total of 2/3 patients had distant metastasis, resulting in a poor prognosis. In a female patient with oral potentially malignant disorder who underwent HSCT, there were 10.7% Y-containing cells in epithelial cells, suggesting that some epithelial cells were from the donor. Subsequently, gene expression patterns in patients with possible BM stem cell-derived OSCC were compared with those in patients with normally developed OSCC by microarray analysis. A total of 3 patients with BM stem cell-derived OSCC exhibited a specific pattern of gene expression. Following cluster analysis by the probes identified on BM stem cell-derived OSCC, 2 patients with normally developed OSCC were included in the cluster of BM stem cell-derived OSCC. If the genes that could discriminate the origin of OSCC were identified, OSCCs were classified into the three aforementioned categories. If diagnosis can be performed based on the origin of the cancer cells, a more specific therapeutic strategy may be implemented to improve prognosis. This would be a paradigm shift in diagnostic and therapeutic strategies for OSCC.

DOI： 10.3892/ol.2021.12431

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

The aim of this study was to analyse the effects of gargling with and then swallowing PPAA (polaprezinc in polyacrylic acid solution), in addition to regular oral management, on patients with a haematopoietic neoplasm scheduled for haematopoietic stem cell transplantation (HSCT). A total of 120 patients scheduled for HSCT during the years 2006-2016 were recruited. Patient background, oral adverse events, the incidence and severity of systemic adverse events (sepsis/septic shock, acute graft-versus-host disease (GVHD) after transplantation), and outcomes (survival/death) were compared between groups treated with and without PPAA. The severities of oral adverse events (oral mucositis, oral pain, and dysgeusia) were significantly lower in patients treated with PPAA. There was no significant difference in the incidence of febrile neutropenia (P=0.622) or sepsis/septic shock (P=0.665) as systemic adverse events. The severity of allograft-induced acute graft-versus-host disease (GVHD) was significantly lower in the PPAA group (P=0.011). There was no significant difference in outcome between the two groups (P=0.285). Within the limitations of the study design, it may be concluded that oral management with PPAA reduces adverse events in HSCT. Oral management with concomitant use of PPAA decreased oral adverse events and reduced the systemic complication of GVHD.

DOI： 10.1016/j.ijom.2020.10.004

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担当区分：筆頭著者,　責任著者   記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

ケモカインレセプターCXCR4を発現する口腔癌細胞が、転移先臓器の産生するリガンドstromal-cell derived factor(SDF)-1に引き寄せられながら転移すること、SDF-1/CXCR4オートクラインループを獲得した細胞は著しい肺転移をすること、これらの転移はCXCR4阻害剤により抑制できることを明らかにした。近年、本システムは癌微小環境にも関与していることが示唆されている。CXCR4の治療標的分子としての可能性を最近の知見を交えながら述べた。

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

Administration of cetuximab (C-mab) in combination with paclitaxel (PTX) has been used for patients with head and neck squamous cell carcinoma (SCC) clinically. In this study, we attempted to clarify the molecular mechanisms of the enhancing anticancer effect of C-mab combined with PTX on oral SCC cells in vitro. We used two oral SCC cells (HSC4, OSC19) and A431 cells. PTX alone inhibited cell growth in all cells in a concentration-dependent manner. C-mab alone inhibited the growth of A431 and OSC19 cells at low concentrations, but inhibited the growth of HSC4 cells very weakly, even at high concentrations. A combined effect of the two drugs was moderate on A431 cells, but slight on HSC4 and OSC19 cells. A low concentration of PTX enhanced the antibody-dependent cellular cytotoxicity (ADCC) induced by C-mab in all of the cells tested. PTX slightly enhanced the anticancer effect of C-mab in this ADCC model on A431 and HSC4 cells, and markedly enhanced the anticancer effect of C-mab on OSC19 cells. These results indicated that PTX potentiated the anticancer effect of C-mab through enhancing the ADCC in oral SCC cells.

DOI： 10.3390/ijms21176292

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Anticancer Research USA Inc.  

AIM: We attempted to clarify the role of Peroxisome proliferator-activated receptor γ (PPARγ) and its ligand, troglitazone (TRO) on oral squamous cell carcinoma (SCC). MATERIALS AND METHODS: The expression of PPARγ gene was examined in 47 human oral SCC tissues and two human oral SCC cell lines, CA9-22 and HSC-4. The effects of TRO on the growth and cell-cycle progression of human oral SCC cells were examined. RESULTS: PPARγ mRNA was detected in 20 of 47 oral SCC tissues and two human oral SCC cells. TRO significantly suppressed the growth of the cells, but did not induce apoptosis. CA9-22 cells treated with TRO showed an increased fraction in the G1 phase and decreased fractions in the S and G2-M phases. CONCLUSION: TRO did not induce apoptosis in oral SCC cells, but did inhibit the growth of the cells by arresting the cell cycle at G1 phase.

DOI： 10.21873/anticanres.14066

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/cas.14359

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Anticancer Research USA Inc.  

BACKGROUND/AIM: Odontogenic diseases are diagnosed based on clinical course, imaging, and histopathology. However, a definitive diagnosis is not always possible. PATIENTS AND METHODS: We analyzed whole exons of SMO, BRAF, PTCH1 and GNAS using next-generation sequencing (NGS) in 18 patients. RESULTS: Of the 6 patients with ameloblastoma, 2 patients had the same missense mutation in BRAF, and 1 patient with peripheral ameloblastoma had a missense mutation in PTCH1. Of the 7 patients with odontogenic keratocyst, 4 patients had a missense mutation in PTCH1, 2 patients had missense mutations in BRAF, and 1 patient had a missense mutation in SMO. The patient with odontoma had missense mutations in SMO, BRAF and PTCH1. One patient with cement-osseous dysplasia had missense mutations in SMO and PTCH1. The patient with adenomatoid odontogenic tumor had missense mutations in SMO. CONCLUSION: Whole exome sequencing of the above genes by NGS would be useful for the differential diagnosis of odontogenic diseases.

DOI： 10.21873/invivo.12159

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.ijom.2019.06.005

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記述言語：日本語   出版者・発行元：(一社)日本口腔腫瘍学会  

近年の治療法の向上にもかかわらず、約30年の間、早期口腔癌の治療成績は改善を認めていない。早期口腔癌における頸部リンパ節転移の有無は最も重要な予後因子である。それゆえに、頸部リンパ節転移の早期発見は予後の改善につながると考えられる。cN0症例における頸部マネージメントについては、経過観察もしくは予防的頸部郭清術のいずれかが選択されてきており、長年の論点となってきた。センチネルリンパ節生検は多くの領域で受け入れられている方法であり、口腔癌においては頸部郭清術の適否を判断する検査法である。本研究ではセンチネルリンパ節生検症例の同定部位、転移陽性レベル等から予防的頸部郭清術の適否について検討を行うとともに、今後の展望について述べる。(著者抄録)

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記述言語：英語  

DOI： 10.1016/j.ajoms.2018.07.009

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/ijc.31811

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Spandidos Publications  

We have previously demonstrated that the stromal cell-derived factor (SDF-1)/CXCR4 system is involved in the metastasis of head and neck cancer. Additionally, it has been revealed that the blockade of CXCR4 by subcutaneous daily injection with AMD3100, a CXCR4 antagonist, may be effective in preventing metastasis in CXCR4-related head and neck cancer. Recent investigations have suggested that AMD070, a novel orally bioavailable inhibitor of CXCR4, may be minimally invasive compared with AMD3100. In the present study, we examined the effect of AMD070 on metastasis induced by the SDF-1/CXCR4 axis in B88-SDF-1 oral cancer cells, which express high levels of SDF-1 and CXCR4. Although treatment with AMD070 did not affect the anchorage-dependent growth of B88-SDF-1 cells, it signifcantly suppressed the anchorage-independent growth. Moreover, the SDF-1/CXCR4-dependent migration and invasion of B88-SDF-1 cells was signifcantly inhibited following treatment with AMD070. Subsequently, we performed an experimental therapy using AMD070 to prevent the distant metastasis of B88-SDF-1 cells in vivo. Daily oral administration of AMD070 signifcantly inhibited the lung metastasis of B88-SDF-1 cells in nude mice. These results indicated that AMD070 could be useful as a novel orally bioavailable inhibitor of oral cancer metastasis.

DOI： 10.3892/or.2018.6400

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier Ltd  

Objective: We examined the symptoms in the oral and maxillofacial regions of patients with IgG4-related disease (IgG4-RD) and considered the meaning of a labial minor salivary gland (LMSG) biopsy in such patients. Methods: Sixty-three patients diagnosed with IgG4-RD in our hospital, were examined for symptoms in the oral and maxillofacial regions. Out of the 24 patients who were referred to our department, 20 underwent immunohistochemical analysis of the LMSG for IgG and IgG4. Results: Symptoms in the oral and maxillofacial region were observed in 18 of the 63 patients. Out of the 18 patients, 15 had swelling of the submandibular glands, 5 had severe xerostomia, 4 had swelling of the parotid glands, and 2 experienced irritation and pain while eating. IgG4-positive plasma cells in the LMSG biopsy samples were observed in 19 of the 20 patients examined (95%). In 6 patients, more than 40% of the infiltrated plasma cells were positive for IgG4
in 2 patients, more than 30% of the plasma cells were positive
in 3 patients, more than 20% of the plasma cells were positive
in 7 patients, more than 10% of the plasma cells were positive
in 1 patient, more than 5% of the plasma cells were positive. Conclusions: Out of the 63 patients, only 18 (29%) had symptoms in the oral and maxillofacial regions. Infiltration of IgG4-positive plasma cells, regardless of their intensity in the non-symptomatic LMSG, might be considered as one of the important symptoms of IgG4-RD.

DOI： 10.1016/j.ajoms.2018.03.004

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DOI： 10.1158/1538-7445.AM2017-1328

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.18632/oncotarget.7308

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

We have previously demonstrated that a stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system is involved in the establishment of metastasis in oral cancer. Recently, small non coding RNAs, microRNAs (miRNAs) have been shown to be involved in the metastatic process of several types of cancers. However, the miRNAs that contribute to metastases induced by the SDF-1/CXCR4 system in oral cancer are largely unknown. In this study, we examined the metastasis-related miRNAs induced by the SDF-1/CXCR4 system using B88-SDF-1 oral cancer cells, which exhibit functional CXCR4 and distant metastatic potential in vivo. Through miRNA microarray analysis, we identified the upregulation of miR-518c-5p in B88-SDF-1 cells, and confirmed the induction by real-time PCR analysis. Although an LNA-based miR-518c-5p inhibitor did not affect cell growth of B88-SDF-1 cells, it did significantly inhibit the migration of the cells. Next, we transfected a miR-518c expression vector into parental B88 cells and CAL27 oral cancer cells and isolated stable transfectants, B88-518c and CAL27-518c cells, respectively. The anchorage-dependent and -independent growth of miR-518c transfectants was significantly enhanced compared with the growth of mock cells. Moreover, we detected the enhanced migration of these cells. The LNA-based miR-518c-5p inhibitor significantly impaired the enhanced cell growth and migration of miR-518c transfectants, indicating that these phenomena were mainly dependent on the expression of miR-518c-5p. Next, we examined the function of miR-518c-5p in vivo. miR-518c transfectants or mock transfectants were inoculated into the masseter muscle or the blood vessels of nude mice. Tumor volume, lymph nodes metastasis, and lung metastasis were significantly increased in the mice inoculated with the miR-518c transfectants. These results indicated that miR-518c-5p regulates the growth and metastasis of oral cancer as a downstream target of the SDF-1/CXCR4 system.

DOI： 10.1371/journal.pone.0115936

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

We have demonstrated that blocking CXCR4 may be a potent anti-metastatic therapy for CXCR4-related oral cancer. However, as CXCR4 antagonists are currently in clinical use to induce the mobilization of hematopoietic stem cells, continuous administration as an inhibitor for the metastasis may lead to persistent leukocytosis. In this study, we investigated the novel therapeutic downstream target(s) of the SDF-1/CXCR4 system, using B88-SDF-1 cells, which have an autocrine SDF-1/CXCR4 system and exhibit distant metastatic potential in vivo. Microarray analysis revealed that 418 genes were upregulated in B88-SDF-1 cells. We identified a gene that is highly upregulated in B88-SDF-1 cells, metabotropic glutamate receptor 5 (mGluR5), which was downregulated following treatment with 1,1'-[1,4-Phenylenebis(methylene)]bis-1,4,8,11-tetraazacyclotetradecane octahydrochloride (AMD3100), a CXCR4 antagonist. The upregulation of mGluR5 mRNA in the SDF-1/CXCR4 system was predominately regulated by the Ras-extracellular signal-regulated kinase (ERK)1/2 pathway. Additionally, the growth of B88-SDF-1 cells was not affected by the mGluR5 agonist (S)-3,5-DHPG (DHPG) or the mGluR5 antagonists 2-Methyl-6-(phenylethynyl)pyridine (MPEP) and 3-((2-Methyl-1,3-thiazol-4-yl)ethynyl)pyridine (MTEP). However, we observed that DHPG promoted B88-SDF-1 cell migration, whereas both MPEP and MTEP inhibited B88-SDF-1 cell migration. To assess drug toxicity, the antagonists were intraperitoneally injected into immunocompetent mice for 4 weeks. Mice injected with MPEP (5 mg/kg) and MTEP (5 mg/kg) did not exhibit any side effects, such as hematotoxicity, allergic reactions or weight loss. The administration of antagonists significantly inhibited the metastasis of B88-SDF-1 cells to the lungs of nude mice. These results suggest that blocking mGluR5 with antagonists such as MPEP and MTEP could prevent metastasis in CXCR4-related oral cancer without causing side effects.

DOI： 10.1371/journal.pone.0080773

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Salivary gland cancers (SGCs) frequently metastasize to cervical lymph nodes and distant organs. Currently, the mechanisms responsible for the metastatic behavior of SGC cells are not fully understood. We previously demonstrated that the stromal cell-derived factor-1 (SDF-1
also known as CXCL12)/CXCR4 system is involved in the establishment of metastasis in oral squamous cell carcinoma. In the present study, we investigated the role of CXCR4 in the metastatic behavior of SGCs. We examined the expression of CXCR4 mRNA and protein in human SGC cell lines by quantitative RT-PCR and western blotting, respectively. The expression of CXCR4 mRNA and protein were frequently upregulated in 5 out of 6 SGC cell lines. Functional CXCR4 expression was demonstrated by the ability of these SGC cell lines to migrate toward an SDF-1 gradient. SDF-1 rapidly activated extracellular signal-regulated kinase (ERK)1/2 in SGC cell lines. Immunohistochemical analysis revealed that CXCR4 protein expression was detected in either the nucleus or cytoplasm of cancer cells in 16 out of 20 tissues of adenoid cystic carcinoma (ACC) and in 4 out of 6 tissues of mucoepidermoid carcinoma, which are representative of SGC. Furthermore, ACC cell lines exhibited dramatic metastasis to the lung following intravenous inoculation, whereas AMD3100, a CXCR4 antagonist, significantly inhibited lung metastasis of the cells, ameliorated body weight loss and improved the survival rate of tumor-bearing nude mice. These results indicate that CXCR4 expression contributes to the metastatic potential of SGCs. © 2012 Springer Science+Business Media B.V.

DOI： 10.1007/s10585-012-9518-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

Parkin is a multifunctional protein, including maintaining mitochondrial homeostasis. Recent evidence suggests that Parkin is recruited from the cytoplasm to damaged mitochondria with low membrane potential. We found that intracellular localization of Parkin changed with cellular growth phase. Parkin was preferentially localized in the mitochondria of cultured cells. The mitochondria with large amounts of Parkin showed preserved membrane potentials even during treatment with carbonyl cyanide m-chlorophenylhydrazone. Here we report a novel protein named Klokin 1 that transports Parkin to the mitochondria. Klokin 1 was localized to the mitochondria and enhanced mitochondrial expression of Parkin. Klokin 1 enhanced cell viability in Parkin-silenced cells. Klokin 1 expression was enhanced in the brains of Parkin-deficient mice but not in an autopsied PARK2 brain. Our findings indicate that mitochondrial Parkin prevents mitochondrial depolarization and that Klokin 1 may compensate for Parkin deficiency.

DOI： 10.1093/hmg/ddr530

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCI LTD  

We have previously demonstrated that a stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system is involved in the establishment of lymph node metastasis in oral squamous cell carcinoma (OSCC). In this study, we investigated whether the blockade of CXCR4 inhibits lymph node metastasis in B88 OSCC cells. These cells harbour a functional CXCR4 and have the potential to metastasise to the lymph node in vivo. Following introduction of a vector that expresses short hairpin small interfering RNA (shRNA) against CXCR4, we isolated three clones (shCXCR4-16, -17 and -21) that showed decreased expression of CXCR4 mRNA. These clones also had reduced CXCR4 protein levels and showed impairments in calcium flux and cell migration in response to SDF-1. These cells were orthotopically inoculated into the masseter muscle of nude mice. Lymph node metastases, loss in body weight and tumour volumes were significantly inhibited in mice inoculated with shCXCR4-17 cells compared to mice inoculated with control cells. SDF-1-induced migration of B88 cells was significantly inhibited in vitro by the treatment with 1,1'-1[4-phenylenebis(methylene)]bis-1,4,8,11-tetraazacyclotetradecane octahydrochloride (AMD3100), a CXCR4 antagonist. Subcutaneous administration of AMD3100 significantly inhibited the lymph node metastases of B88 cells when they were orthotopically inoculated into the masseter muscle of nude mice. Moreover, the enhanced production of interleukin (IL)-6 and IL-8 in response to SDF-1 was inhibited by shRNA against CXCR4 or by treatment with AMD3100. These results suggest that blockade of CXCR4 may be a potent anti-metastatic therapy against lymph node metastases in cases of CXCR4-related OSCC. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.ejca.2010.09.028

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1186/1476-4598-8-62

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

We have previously shown that a stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system is involved in the establishment of lymph node metastasis, but not in that of distant metastasis, in oral squamous cell carcinoma (SCC). In this study, we investigated the role of the autocrine SDF-1/CXCR4 system, with a focus on distant metastasis in oral SCC cells. The immunohistochemical staining of SDF-1 and CXCR4 using primary oral SCCs and metastatic lymph nodes showed a significantly higher number of SDF-1-positive cases among the metastatic lymph nodes than among the primary oral SCCs, which was associated with a poor survival rate among those of the former group. The forced expression of SDF-1 in B88 cells, which exhibit functional CXCR4 and lymph node metastatic potential (i.e., the autocrine SDF-1/CXCR4 system), conferred enhanced cell motility and an chorage-independent growth potential onto the cells. Orthotopic inoculation of the transfectant into nude mice was associated with an increase in the number of metastatic lymph nodes and more aggressive metastatic foci in the lymph nodes. Furthermore, the SDF-1 transfectant (i.e., the autocrine SDF-1/CXCR4 system) exhibited dramatic metastasis to the lung after im. inoculation, whereas the mock transfectant (i.e., the paracrine SDF-1/CXCR4 system) did not. Under the present conditions, AMD3100, a CXCR4 antagonist, significantly inhibited the lung metastasis of the SDF-1 transfectant, ameliorated body weight loss, and improved the survival rate of tumor-bearing nude mice. These results suggested that, in cases of oral SCC, the paracrine SDF-1/CXCR4 system potentiates lymph node metastasis, but distant metastasis might require the autocrine SDF-1/CXCR4 system.

DOI： 10.1158/1541-7786.MCR-06-0368

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

A p53 functional analysis system, which can identify the types of abnormality of p53, such as loss of function, dominant negative function, or gain of oncogenic function, is now required. In this study, we examined the functional diversity of several mutations of p53 derived from human head and neck cancer cells. The entire open reading frame of p53 cDNA was subcloned into a mammalian expression vector, pEGFP-C3, and genetic mutations were determined. Then, intracellular localization and transcriptional activity of the tumor-derived p53 proteins were examined in Saos-2 cells. A mutant-p53 (Glu17Lys, His193Leu) or a truncated p53 (Delta 121) did not activate the reporters containing p53 responsive elements from p21waf1, BAX, MDM2, p53AIP1, and PUMA genes at all. However, a mutant-p53 (Asn30Ser) showed the transcriptional activity on all of the reporters as wild-type p53 did. On the other hand, a mutant-p53 (Asp281His) activated the p21waf1 promoter strongly and the MDM2 promoter faintly, but did not activate the BAX promoter. Interestingly, this mutant-p53 prevented Saos-2 cells from undergoing apoptosis after treatment with a DNA damaging agent, adriamycin. This mutant-p53 induced cell cycle arrest but not apoptosis. Furthermore, another mutant-p53 (Glu17Lys, His193Leu) also prevented the cells from undergoing apoptosis after DNA damage probably in a transcription-independent manner. These results suggest that some cancer cells may contain the oncogenic mutation of the p53 gene, and the oncogenic p53 protein prevents cancer cells from undergoing apoptosis after DNA damage. Detailed information for mutated p53 gene in cancer cells might provide useful suggestions for the therapeutic strategy.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPANDIDOS PUBL LTD  

Epithelial-mesenchymal transition (EMT) refers to critical events occasionally observed during tumor progression, including invasion and metastasis, by which cancer cells acquire a fibroblast-like phenotype. Since the stromal cell-derived factor-1 (SDF-1)/CXCR4 system can facilitate lymph node metastasis in oral squamous cell carcinoma (SCC), we have explored the possibility that this system might be involved in EMT. Oral SCC cells, B88 and HNt, which have functional CXCR4 and lymph node metastatic potential, were found to lose their epithelial cell morphology due to SDF-1. In this context, the downregulation of epithelial markers, cytokeratin, E-cadherin and beta-catenin, and the upregulation of mesenchymal marker, vimentin and snail were detected. Furthermore, upregulation of vimentin by treatment with SDF-1 was impaired by phosphatidylinositol 3 kinase (PI3K) inhibitor Wortmannin, but not by mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor U0126. In the type I collagen embedding culture, SDF-1-treated B88 cells formed protruding extensions, but the effect was impaired by treatment with Wortmannin. These results suggested that EMT induced by the SDF-1/CXCR4 system might be involved in the lymph node metastasis of oral SCCs via activation of PI3K-Akt/PKB pathway.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER ASSOC IMMUNOLOGISTS  

Autoimmune regulator (AIRE) gene mutation is responsible for the development of organ-specific autoimmune disease with monogenic autosomal recessive inheritance. Although Aire has been considered to regulate the elimination of autoreactive T cells through transcriptional control of tissue-specific Ags in thymic epithelial cells, other mechanisms of AIRE-dependent tolerance remain to be investigated. We have established Aire-deficient mice and examined the mechanisms underlying the breakdown of self-tolerance. The production and/or function of immunoregulatory T cells were retained in the Aire-deficient mice. The mice developed Sjogren's syndrome-like pathologic changes in the exocrine organs, and this was associated with autoimmunity against a ubiquitous protein, a-fodrin. Remarkably, transcriptional expression of alpha-fodrin was retained in the Aire-deficient thymus. These results suggest that Aire regulates the survival of autoreactive T cells beyond transcriptional control of self-protein expression in the thymus, at least against this ubiquitous protein. Rather, Aire may regulate the processing and/or presentation of self-proteins so that the maturing T cells can recognize the self-Ags in a form capable of efficiently triggering autoreactive T cells. With the use of inbred Aire-deficient mouse strains, we also demonstrate the presence of some additional factor(s) that determine the target-organ specificity of the autoimmune disease caused by Aire deficiency.

DOI： 10.4049/jimmunol.174.4.1862

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

Recently, it has been suggested that chemokine/receptor interactions determine the destination of the invasive tumor cells in several types of cancer. It has also been proposed that the stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system might be involved lymph node metastasis in oral squamous cell carcinoma (SCC). In order to further clarify the role of the SDF-1/CXCR4 system in oral SCC, we generated CXCR4 stable transfectants (IH-CXCR4) using oral SCC cells, and compared them to IH, which did not express CXCR4 and which did not have lymph node metastatic potentials in vivo. We introduced enhanced green fluorescent protein (GFP) fused-CXCR4 into IH cells, and detected the GFP fluorescence in the cytoplasm and cell membrane in approximately 60% of the G418-resistant cells. This bulk-transfectant expressed a high level of CXCR4 mRNA and protein, and exhibited the characteristic calcium fluxes and chemotactic activity observed in treatment with SDF-1. SDF-1 biphasically activated extracellular signal-regulated kinase (ERK)1/2, but continuously activated Akt/protein kinase B (PKB) in IH-CXCR4 cells. Most importantly, IH-CXCR4 cells frequently metastasized to the cervical lymph node, but not to the distant organs in the orthotopic inoculation of nude mice. Furthermore, these lymph node metastases were inhibited by the treatment of a mitogen-activated protein kinase/ERK kinase inhibitor, U0126, or a phosphatidylinositol 3 kinase inhibitor, wortmannin. These results indicate that SDF-1/CXCR4 signaling mediates the establishment of lymph node metastasis in oral SCC via ERK1/2 or Akt/PKB pathway.

DOI： 10.1038/labinvest.3700190

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

14-3-3 sigma, a target gene of the p53 tumour suppressor protein, has been shown to regulate the cell cycle at the G2/M checkpoint. Recent studies have demonstrated that 14-3-3 sigma is downregulated by hypermethylation of the CpG island in several types of cancer. In this study, we investigated the expression and methylation status of 14-3-3 sigma in human salivary gland adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (MEC). Immunohistochemical analysis revealed that the positive expression rate of 14-3-3 sigma in ACC ( one out of 14) was markedly lower than that in MEC ( ten out of 10). Since most of the ACCs carried the wild-type p53 protein, downregulation of 14-3-3 sigma in ACC may not be due to the dysfunction of p53 pathway. Microdissection - methylation-specific PCR revealed that frequent hypermethylation of the 14-3- 3 sigma gene was observed in ACC when compared to that in MEC. In cultured-ACC cells, we confirmed the downregulation of 14-3- 3 sigma via hemimethylation of the gene by sequencing analysis after sodium bisulphite treatment. Furthermore, re-expression of 14-3- 3 s in the ACC cells was induced by the treatment with DNA demethylating agent, 5-aza-2'-deoxycytidine. Irradiation apparently induced the enhanced expression of 14-3- 3 sigma and G2/M arrest in normal salivary gland cells; however, in the ACC cells, neither induction of 14-3- 3 sigma nor G2/M arrest was induced by irradiation. These results suggest that downregulation of 14-3- 3 sigma might play critical roles in the neoplastic development and radiosensitivity of ACC.

DOI： 10.1038/sj.bjc.6602004

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

Autoimmune regulator ( AIRE) is responsible for the development of organ-specific autoimmune disease in a monogenic fashion. Rare and low levels of tissue expression together with the lack of AIRE-expressing cell lines have hampered a detailed analysis of the molecular dynamics of AIRE. Here we have established cell lines stably transfected with AIRE and studied the regulatory mechanisms for its subcellular expression. We found that nuclear body ( NB) formation by AIRE was dependent on the cell cycle. Biochemical fractionation revealed that a significant proportion of AIRE is associated with the nuclear matrix, which directs the functional domains of chromatin to provide sites for gene regulation. Upon proteasome inhibition, AIRE NBs were increased with concomitant reduced expression in the cytoplasm, suggesting that subcellular targeting of AIRE is regulated by a ubiquitin-proteasome pathway. We also found that AIRE NBs compete for cAMP-response element-binding protein-binding protein/p300, a common coactivator of transcription, with the promyelocytic leukemia gene product. These results suggest that the transcriptional regulating activities of AIRE within a cell are controlled and organized in a spatiotemporal manner.

DOI： 10.1074/jbc.M400702200

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

We have demonstrated the possibility that the stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system might be involved in the establishment of lymph node metastasis in oral squamous cell carcinoma (SCC). In order to further clarify the role of the SDF-1/CXCR4 system in oral SCC, we examined the expression of CXCR4 and SDF-1 in biopsy specimens from 61 patients with oral SCC by means of immunohistochemistry, and investigated several clinicopathological factors, including age, sex, lymph node metastasis, invasion, recurrence and prognosis. The expression of CXCR4 and SDF-1 was observed in 57.3 and 11.4% of our subjects, respectively. Although we were unable to find a statistically significant association between the expression of SDF-1 and any clinicopathological factors, we did find a significant association between the expression of CXCR4 and lymph node metastasis (P = 0.0417). Moreover, the mode of invasion (P = 0.0002) and recurrence of the tumors (P = 0.0185) were strongly associated with CXCR4 expression, and the CXCR4-positive group showed a significantly poorer prognosis than the CXCR4-negative group (P = 0.0401). Recombinant SDF-1alpha stimulated in vitro invasiveness and scattering in CXCR4-expressing oral SCC cells, but the treatment did not affect the expression of matrix metalloproteinases or urokinase-type plasminogen activator. These results indicate that SDF-1/CXCR4 signaling in oral SCC cells might be involved in the diverse action of oral SCC, including invasion or micrometastasis at the primary site and lymph node metastasis.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ROCKEFELLER UNIV PRESS  

Autoimmune regulator (AIRE) gene mutation is responsible for the development of autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy, an organ-specific autoimmune disease with monogenic autosomal recessive inheritance. AIRE is predominantly expressed in medullary epithelial cells of the thymus and is considered to play important roles in the establishment of self-tolerance. AIRE contains two plant homeodomain (PHD) domains, and the novel role of PHD as in E3 ubiquitin (Ub) ligase has just emerged. Here we show that the first PHD (PHD1) of AIRE mediates E3 ligase activity. The significance of this finding was underscored by the fact that disease-causing missense mutations in the PHD 1 (C311Y and P326Q) abolished its E3 ligase activity. These results add a novel enzymatic function for AIRE and suggest an indispensable role of the Ub proteasome pathway in the establishment of self-tolerance, in which AIRE is involved.

DOI： 10.1084/jem.20031291

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

We examined the role of chemokine signaling on the lymph node metastasis of oral squamous cell carcinoma (SCC) using lymph node metastatic (HNt and B88) and nonmetastatic oral SCC cells. Of 13 kinds of chemokine receptors examined, only CXCR4 expression was up-regulated in HNt and B88 cells. CXCR4 ligand, stromal-cell-derived factor-1alpha (SDF-1alpha; CXCL12), induced characteristic calcium fluxes and chemotaxis only in CXCR4-expressing cells. CXCR4 expression in metastatic cancer tissue was significantly higher than that in nommetastatic cancer tissue or normal gingiva. Although SDF-1alpha was undetectable in either oral SCC or normal epithelial cells, submandibular lymph nodes expressed the SDF-1alpha protein, mainly in the stromal cells, but occasionally in metastatic cancer cells. The conditioned medium from lymphatic stromal cells promoted the chemotaxis of B88 cells, which was blocked by the CXCR4 neutralization. SDF-1alpha rapidly activated extracellular signal-regulated kinase (ERK)1/2 and Akt/protein kinase B (PKB), and their synthetic inhibitors attenuated the chemotaxis by SDF-1alpha. SDF-1alpha also activated Src family kinases (SFKs), and its inhibitor PP1 diminished the SDF-1alpha-induced chemotaxis and activation of both ERK1/2 and Akt/PKB. These results indicate that SDF-1/CXCR4 signaling may be involved in the establishment of lymph node metastasis in oral SCC via activation of both ERK1/2 and Akt/PKB induced by SFKs. (C) 2003 Elsevier Inc. All rights reserved.

DOI： 10.1016/S0014-4827(03)00344-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

TSC-22 gene was composed of three exons and its length was approximately 5.5 kb including 2.9 kb promoter region. The transcription starting site was located at 7 and 29 bp downstream from TATA box. Promoter analysis revealed that 2146 bp of TSC-22 promoter was activated by several differentiation inducing drugs. Although originally TSC-22 was isolated as a TGF-beta-inducible gene, TSC-22 promoter was not activated by the enhanced TGF-beta signaling. We found 3 copies of the Shaw-Kamens sequence (AUUUA) in the human TSC-22 mRNA 3'-UTR and identified three proteins (40, 20, and 15 kDa) which bound to this. Only the 40 kDa protein-RNA complex was decreased by treatment with TGF-beta1. Moreover, the TSC-22 mRNA 3'-UTR destabilized the heterologous luciferase mRNA, but the destabilization was recovered with TGF-beta1. These observations suggest that up-regulation of TSC-22 mRNA by TGF-beta1 is achieved by mRNA stabilization, but not by transcriptional activation. (C) 2003 Elsevier Science (USA). All rights reserved.

DOI： 10.1016/S0006-291X(03)00854-4

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：APSIT ASSOC PROM STUD IMMUNOL TUMOR  

Recently, a new concept for cancer therapy termed "tumor dormancy therapy" has been proposed. The concept of this therapy is to prolong the survival time of cancer patients while maintaining their quality of life. We have been developing a differentiation-inducing therapy, which is included in the tumor dormancy therapy, for salivary gland cancer. In this study, we examined the effect of a differentiation-inducing drug, Vesnarinone on the growth of several cancer cells, and examined the molecular mechanism by which Vesnarinone induces the cyclin dependent kinase inhibitor, p21(waf1) in the cancer cells. Vesnarinone significantly suppressed the growth of TYS (salivary gland cancer cells), PC3 (prostate cancer cells), and A431 (squamous cell cancer cells). Furthermore, Vesnarinone dose-dependently enhanced the expression of p21(waf1) mRNA in TYS cells. Using the luciferase reporter assay it was found that the enhancement of p21(waf1) mRNA expression by Vesnarinone was through direct transcriptional activation of the p21(waf1) promoter. Thus, analyzing the molecular mechanisms of differentiation inducing drugs may lead to the development of a new therapeutic strategy for several human malignancies, including salivary gland cancer.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

We previously demonstrated that a differentiation inducing drug, vesnarinone induced the growth arrest and p21(waf1) gene expression in a human salivary gland cancer cell line, TYS. In the present study, we investigated the mechanism of the induction of P21(waf1) gene by vesnarinone in TYS cells. We constructed several reporter plasmids containing the p21(waf1) promoter, and attempted to identify vesnarinone-responsive elements in the p21(waf1) promoter. By the luciferase reporter assay, we identified the minimal vesnarinone-responsive element in the p21(waf1) promoter at -124 to -61 relative to the transcription start site. Moreover, we demonstrated by electrophoretic mobility shift assay that Sp1 and Sp3 transcription factors bound to the vesnarinone-responsive element. Furthermore, we found that vesnarinone induced the histone hyperacetylation in TYS cells. These results suggest that vesnarinone directly activates p21(waf1) promoter via the activation of Sp1 and Sp3 transcription factors and the histone hyperacetylation in TYS cells. (C) 2002 Cancer Research UK.

DOI： 10.1038/sj.bjc.6600592

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

It is important to examine the abnormality of the entire p53 tumor suppressor pathway in head and neck cancer. We examined the mRNA expressions of p53 regulatory factors, p33ING1 and p14ARF, and a p53-target gene, p21WAF1 in head and neck cancer, Nine of 14 benign pleomorphic adenomas (PAs) and 7 of 8 malignant salivary gland tumors (MSGTs) expressed p33ING1 mRNA. Thirteen of 14 PAs expressed p14ARF mRNA, however, only I of 8 MSGTs expressed p14ARF mRNA. Eight of 14 PAs and 7 of 8 MSGTs expressed p21WAF1 mRNA. In salivary gland tumors, there was clear correlation between the expression of p33ING1 and p21WAF1 (p<0.0001, r(2)=0.53). However, there was no correlation between the expression of p14ARF and p21WAF1 (p=0.6543, r(2)=0.009). Twenty-six of 28 oral squamous cell carcinomas (SCCs) expressed p33ING1 mRNA. Nineteen of 28 oral SCCs expressed p14ARF mRNA. All of the oral SCCs expressed p21WAF1 mRNA. In oral SCCs, the expressions of both p33ING1 (p=0.009, (r(2)=0.181) and p14ARF(p=0.0009, r(2)=0.271) correlated with the expression of p21WAF1. Interestingly, 24 of 26 oral SCCs (92%) showed either abnormality of p53 itself or loss of expression of p53 regulatory factors, p33ING or p14ARF. These results suggest that head and neck cancer often involve the dysfunction of p53 tumor suppressor pathway.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

We transfected a salivary gland cancer cell line, TYS, with three different forms of TSC-22 (transforming growth factor-beta-stimulated clone-22) gene: full-length TSC-22 (TSC-22FL) containing nuclear export signal, TSC-box and leucine zipper, truncated TSC-22 (TSC-22LZ) containing only TSC-box and leucine zipper, and truncated TSC-22 with nuclear localization signal (NLS-TSC-22LZ). High expression of TSC-22FL in the cytoplasm markedly enhanced the radiation-sensitivity of TYS cells, while, moderate expression of TSC-22FL marginally affected the radiation-sensitivity. TSC-22LZ, which was expressed in the cytoplasm and the nucleus, enhanced the radiation-sensitivity of TYS cells irrespective to its expression level. N-LS-TSC-22LZ, which was expressed only in the nucleus, marginally affected the radiation-sensitivity of the cells even at high expression level. Interestingly, cytoplasmic TSC-22 translocates to nucleus concomitant with radiation-induced apoptosis. These results suggest that cytoplasmic localization of TSC-22 and translocation of TSC-22 from cytoplasm to nucleus is important for regulating the cell death signal after irradiation-induced DNA damage. (C) 2002 Elsevier Science (USA).

DOI： 10.1006/bbrc.2002.6776

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

Peroxisome proliferator-activated receptor gamma (PPARgamma) is a member of the nuclear receptor superfamily of ligand-activated transcription factors. It is expressed in several tissue types, including adipose tissue in which it stimulates adipogenesis. Recent studies have demonstrated that PPARgamma ligands induce cellular differentiation and inhibit cell growth in carcinomas of various organs including the breast, prostate, lung, colon, stomach, bladder, and pancreas. However, whether PPARgamma is expressed in human salivary gland tumors and its function in this tissue is unknown. In the present study, we examined PPARgamma gene expression in human salivary gland cancer cells and tested its ligands for any antitumor effect. PPARgamma mRNA was detected by RT-PCR in both benign and malignant salivary gland tumor tissues. The effect of PPARgamma on cell growth was investigated using four human salivary gland cancer cell lines; HSG, AZA3, HSY and TYS, which were confirmed to express PPARgamma1 mRNA and protein. Retinoid X receptor a protein, which forms heterodimers with PPARgamma, was also detected in all the cells tested. Data obtained by luciferase assay indicated that the intrinsic PPARgamma protein was activated by the synthetic ligands, troglitazone and pioglitazone, but not by the natural ligand, 15-deoxy-Delta(12), (14)-prostaglandin J(2). The synthetic PPARgamma ligands, particularly troglitazone, caused significant dose-dependent inhibition of cancer cell growth. Furthermore, the overexpression of PPARgamma1 or PPARgamma2 in cancer cells also reduced significantly their growth rate. These results suggest that PPARgamma and its synthetic ligands suppress the growth of human salivary gland cancer cells and it may be a useful molecular target for cancer treatment.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

Several investigators have demonstrated that TGF-beta stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-22 and the nuclear translocation of TSC-22 concomitant with induction of apoptosis in salivary gland cancer cells. In the present study, we attempted to identify the active domain of TSC-22 protein that regulated the biological functions of TSC-22. We constructed three mammalian expression vectors, which could produce full length TSC-22 only in cytoplasm, the leucine zipper structure of TSC-22 in both cytoplasm and nucleus, and the leucine zipper structure only in nucleus. Then, we transfected a salivary gland cancer cell line, HSG with these expression vectors, and investigated the growth profile of the transfectants. None of the TSC-22 constructs inhibited the monolayer growth and the anchorage-dependent colony formation of HSG cells. However, the leucine zipper structure of TSC-22 markedly inhibited the anchorage-independent colony formation of HSG cells (p<0.001; one way ANOVA). Full length TSC-22 also suppressed the anchorage-independent colony formation of HSG cells, although the effect of full length TSC-22 was much lower than those of the leucine zipper constructs. These observations suggest that the leucine zipper structure in TSC-22 protein is an active domain that negatively regulates the growth of salivary gland cancer cells.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

The measurement of the intra-tumoral level of thymidylate synthetase (TS), and dihydropyrimidine dehydro-genase (DPD), may be useful in predicting tumor sensitivity to 5-fluorouracil (5-FU). In this study, we examined the mRNA levels of DPD and TS in 28 oral squamous cell carcinomas (SCC), and 22 salivary gland tumors by semiquantitative reverse transcription polymerase chain reaction. Then we examined the correlation of the responsiveness of the patients with oral SCC to 5-FU with the intra-tumoral levels of DPD and TS mRNA. All specimens were obtained at the biopsy before treatment, and then the patients were treated by oral administration of a 5-FU compound (UFT), the irradiation of cobatt-60 (upto 60 Gy) and injection of an immuno-potentiator (OK-432). Intra-tumoral levels of DPD mRNA in the patients who showed CR (complete response) and PR (partial response) were significantly lower than those in the patients who showed NC (no change). However, intratumoral levels of DPD mRNA did not correlate with the local recurrence of the tumor during the observation period after initial treatment with or without surgical resection of the residual tumors. On the other hand, TS mRNA levels in the tumors did not correlate with any clinico-pathological parameters. These observations suggest that intra-tumoral levels of DPD mRNA may predict the tumor response to 5-FU-based chemo-immuno-radiation therapy in the patients with oral SCC.

DOI： 10.3892/ijo.19.5.953

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

We examined the role of the hepatocyte growth factor (HCF)/c-met system on invasion and metastasis of oral squamous cell carcinoma (SCC) cells. In monolayer culture, exogenous HCF marginally affected the growth of oral SCC cells (BHY, HN, IH) and human gingival epithelial cells (CE), In type I collagen matrix, however, HGF significantly enhanced the invasive growth of the cancer cells (p < 0.05), We detected the expression of c-met (HGF receptor) mRNA in all of the cancer cells, but nor in human gingival fibroblasts (GF), Oral SCC cells did not secret HCF protein into the medium, but GF secreted a large amount of HGF protein (15 ng/ml), Furthermore, HCF markedly enhanced the migration of cancer cells in a Transwell invasion chamber. Then, we examined the serum levels of HGF in oral SCC patients, or HGF concentrations in oral cancer tissues. Serum levels of HCF in the patients were significantly higher than those in healthy volunteers (p < 0.05), After initial treatment, all of the tumor-free survivors showed a marked decline in the serum HCF levels. Furthermore, HGF concentrations in metastatic cancer tissues were significantly higher than those of nonmetastatic cancer tissues and normal gingiva (p < 0.01). These results suggest that HGF plays an important role in invasion and metastasis of oral SCC cells as a paracrine factor, and an elevated HCF level in the cancer tissue can be a predictive marker for metastasis formation in patients with oral SCC. (C) 2001 Wiley-Liss, Inc.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CHURCHILL LIVINGSTONE  

Retinoids inhibit the proliferation of several types of tumour cells, and are used for patients with several malignant tumours. In this study, we examined the effect of retinoic acids (RAs) on the invasive potentials of the oral squamous cell carcinoma (SCC) cells, BHY and HNt. BHY cells expressed all of retinoid nuclear receptors (RAR alpha, beta, gamma, and RXR alpha) and cytoplasmic retinoic acid binding proteins (CRABP1 and CRABP2). HNt cells lacked the expression of RAR beta, but expressed other nuclear receptors and CRABPs. All-trans retinoic acid (ATRA) and 13-cis retinoic acid (13-cisRA) (10(-6) and 10(-7) M) inhibited the growth of the cells, but low-dose ATRA and 13-cisRA (10(-8) M) marginally affected the growth of the cells. Surprisingly. low-dose RAs enhanced the activity of tissue-type plasminogen activator (tPA), and activated pro-matrix metalloproteinases (proMMP2 and proMMP9). Activation of proMMP2 and proMMP9 was inhibited by aprotinin, a serine-proteinase, tPA inhibitor. Furthermore, low-dose RAs enhanced the in vitro invasiveness of BHY cells. These results indicate that low-dose RAs enhances the in vitro invasiveness of oral SCC cells via an activation of proMMP2 and proMMP9 probably mediated by the induction of tPA. (C) 2001 Cancer Research Campaign http://www.bjcancer.com.

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Rockefeller University Press  

Both nuclear factor (NF)-κB–inducing kinase (NIK) and inhibitor of κB (IκB) kinase (IKK) have been implicated as essential components for NF-κB activation in response to many external stimuli. However, the exact roles of NIK and IKKα in cytokine signaling still remain controversial. With the use of in vivo mouse models, rather than with enforced gene-expression systems, we have investigated the role of NIK and IKKα in signaling through the type I tumor necrosis factor (TNF) receptor (TNFR-I) and the lymphotoxin β receptor (LTβR), a receptor essential for lymphoid organogenesis. TNF stimulation induced similar levels of phosphorylation and degradation of IκBα in embryonic fibroblasts from either wild-type or NIK-mutant mice. In contrast, LTβR stimulation induced NF-κB activation in wild-type mice, but the response was impaired in embryonic fibroblasts from NIK-mutant and IKKα-deficient mice. Consistent with the essential role of IKKα in LTβR signaling, we found that development of Peyer's patches was defective in IKKα-deficient mice. These results demonstrate that both NIK and IKKα are essential for the induction of NF-κB through LTβR, whereas the NIK–IKKα pathway is dispensable in TNFR-I signaling.

DOI： 10.1084/jem.193.5.631

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC  

We examined the alteration of the subcellular localization of TSC-22 (TGF-beta -stimulated clone-22) after induction of apoptosis and the transcription-regulatory activity of TSC-22. In the living cells, TSC-22-green fluorescent protein (GFP) fusion protein was clearly localized to the cytoplasm, however, in the apoptotic cells, the TSC-22-GFP fusion protein was translocated from the cytoplasm to the nucleus. TSC-22 fused to GAL4-DNA binding domain (GAL4BD) did not show the transcriptional activity on the reporter genes in yeast and in HSG (salivary gland cancer cells) and Hela. However, in CHO cells, TSC-22-GAL4BD fusion protein strongly activated the reporter gene. The transcriptional activity of the leucine zipper structure of TSC-22 is greater than that of the full-length TSC-22. These findings suggest that after receiving the apoptotic stimuli, TSC-22 translocates from the cytoplasm to the nucleus and shows the transcription-regulatory activity. (C) 2000 Academic Press.

DOI： 10.1006/bbrc.2000.3840

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掲載種別：研究論文（学術雑誌）   出版者・発行元：The American Association of Immunologists  

DOI： 10.4049/jimmunol.165.2.804

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

We have isolated transforming growth factor-beta-stimulated clone-22 (TSC-22) cDNA as an anti-cancer drug-inducible gene in a human salivary gland cancer cell line, TYS. We have previously reported that TSC-22 negatively regulates the growth of TYS cells, and that overexpression of TSC-22 protein in TYS cells enhanced the in vitro chemosensitivity of the cells. In this study, we examined the in vivo chemosensitivity of TSC-22-expressing TYS cells. TSC-22-expressing TYS cells formed tumors in nude mice, but tumors formed by TSC-22-expressing TYS cells were significantly smaller than tumors formed by control cells (p<0.001, one way ANOVA). Furthermore, intraperitoneal injection of 5-fluorouracil (5-FU) markedly inhibited the growth of the TSC-22-expressing TYS tumors, but did not affect the growth of control tumors. It was found by TUNEL assay that TSC-22-expressing TYS tumors were induced to undergo apoptosis by 5-FU treatment. These findings suggest that overexpression of TSC-22 protein in TYS cells enhances the in vivo chemosensitivity of the cells to 5-FU via induction of apoptosis.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：LIPPINCOTT WILLIAMS & WILKINS  

We have recently isolated TSC-22 (transforming growth factor-beta-stimulated clone-22) cDNA as an anticancer, drug-inducible (with vesnarinone) gene in a human salivary gland cancer cell line, TYS. We have also reported that TSC-22 negatively regulates the growth of TYS cells and that down-regulation of TSC-22 in TYS cells plays a major role in salivary gland tumorigenesis (Nakashiro et at, 1998). In this study, we transfected TYS cells with an expression Vector encoding the TSC-22-GFP (green fluorescent protein) fusion protein, and we established TSC-22-GFP-expressing TYS cell clones. Next, we examined (a) the subcellular localization of the fusion protein, (b) the sensitivity of the transfectants to several anticancer drugs (5-fluorouracil, cis-diaminedichoroplatinum, peplomycin), and (c) induction of apoptotic cell death in the transfectants by 5-fluorouracil treatment. The TSC-22-GFP fusion protein was clearly localized to the cytoplasm, but not to the nucleus. Over-expression of the TSC-22-GFP fusion protein did not affect cell growth, but significantly increased the sensitivity of the cells to the anticancer drugs (p < 0.01; one-way ANOVA). Furthermore, over-expression of the TSC-22-GFP fusion protein markedly enhanced 5-fluorouracil-induced apoptosis. These findings suggest that over-expression of TSC-22-GFP protein in PIS cells enhances the chemosensitivity of the cells via induction of apoptosis.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

Epidermal growth factor (EGF) is a potent mitogen, and its action is mediated by MAP kinase (MAPK). Reportedly EGF activates STAT, induces the expression of p21(waf1), and subsequently inhibits the growth of several types of cancer cells. In this study, we used human bladder cancer cells (T24 and RT4), immortalized non-tumorigenic human urothelial cells (1T-1, 1T-2, and 1T-3), and epidermal carcinoma cells (A431). EGF inhibited the growth of T24 and A431, and stimulated the growth of 1T-1, 1T-3 and 1T-2, but did not affect the growth of RT4. EGF activated MAPK strongly in 1T-1, and slightly in A431, T24, 1T-2, and 1T-3 but marginally in RT4. We detected the activation of STAT in T24, 1T-3 and A431 after EGF treatment. EGF enhanced the expression of p21(waf1) mRNA in T24, 1T-2, 1T-3 and A431, and activated the p21(waf1) promoter in T24 cells. These results suggest that i) EGF inhibits the growth of T24 cells via induction of p21(waf1) mediated by STAT, and ii) the balance between the STAT-induced p21(waf1) and MAPK activities regulates the growth of human bladder cells after EGF treatment.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CHURCHILL LIVINGSTONE  

We examined the expression of cytokeratin 20 (CK20) mRNA in the peripheral blood of oral squamous cell carcinoma (SCC) patients by reverse transcriptase polymerase chain reaction (RT-PCR). Eleven out of 12 oral SCC patients showed positive RT-PCR results. However, there is no clear relationship between the haematogenous CK20 mRNA and the metastasis. After initial treatment, all of the tumour-free survivors tested showed negative RT-PCR results. CK20 mRNA in peripheral blood can be used as a marker for tumour recurrence but not for metastasis in oral SCC patients.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PROFESSOR D A SPANDIDOS  

We examined the gelatinolytic activity in human oral squamous-cell carcinoma tissues in order to evaluate the capability of intravasation and extravasation of cancer cells. By a microdissection-zymography, we demonstrated separately the gelatinolytic activities in cancer cell nests and stroma adjacent to the cancer cells. The gelatinolytic activities, such as pro-matrix metalloproteinase (MMP)9 and active-MMP2 in most of cancer cell nests were much higher than those of normal gingival epithelium. Moreover, the activities of active-MMP2 in cancer cell nests tf metastatic cancers were significantly higher than those of non-metastatic cancers (p<0.05). These results suggest that active-MMP2 in cancer cells can be a predictive marker for metastasis formation in oral squamous-cell carcinoma patients.

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PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

We have recently isolated TSC-22 (transforming growth factor beta-stimulated clone 22) cDNA as a new anticancer drug (Vesnarinone)-inducible gene in a human salivary gland cancer cell line, TYS. We conducted the present study to examine whether up-regulation or down-regulation of TSC-22 can affect the growth of TYS cells in vitro and in vivo. We constructed an expression vector containing sense-or antisense-oriented human TSC-22 cDNA under the transcriptional control of the SR alpha promoter. We cotransfected TYS cells with the sense or antisense expression vector and pSV2neo and obtained more than 200 G418-resistant colonies in each sense or antisense transfectant. Approximately 80% of representative G418-resistant clones expressed the transcripts from transfected sense or antisense TSC-22 cDNA. To avoid the clonal heterogeneity of the cells, we mixed all of the G418-resistant colonies together in each sense or antisense transfectant and examined the expression of TSC-22 protein, in vitro growth, and the tumorigenicity in nude mice. The expression of TSC-22 protein was examined by solid-phase ELISA using a specific antibody against recombinant TSC-22 protein. The expression of TSC-22 protein was up-regulated in the sense transfectants and down-regulated in the antisense transfectants. Contrary to our expectation, up-regulation of TSC-22 protein did not affect both in vitro and in vivo growth of TYS cells, However, do cvn-regulation of TSC-22 markedly enhanced the growth of TYS cells in vitro and in vivo., Furthermore, we examined the expression of TSC-22 mRNA in several human salivary gland tumors, The mRNA expression of TSC-22 in benign and malignant salivary gland tumors was significantly decreased when compared to that in tumor-free salivary glands (P < 0.05; one-way ANOVA), and in some salivary gland tumors, the expression of TSC-22 mRNA was not detectable by reverse transcription-PCR, These results suggest that down-regulation of TSC-22 may play a major role on salivary gland tumorigenesis.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CHURCHILL LIVINGSTONE  

We undertook the present study to clarify the molecular mechanism of the effect of a new anti-cancer drug, vesnarinone, on a human salivary gland cancer cell line, TYS. We isolated TSC-22 cDNA as a vesnarinone-inducible gene from a cDNA library constructed from vesnarinone-treated TYS cells. TSC-22 was originally reported as a transforming growth factor (TGF)-beta-inducible gene. The expression of TSC-22 was up-regulated within a few hours after treatment with vesnarinone and was continued for 3 days. The level of TSC-22 mRNA in PIS cells was continuously increased until the cells reached confluency. Furthermore, the induction of TSC-22 by vesnarinone was inhibited by treatment with cycloheximide. When we treated the cells with an antisense oligonucleotide against TSC-22 mRNA under quiescent conditions, the antisense oligonucleotide stimulated the growth of TYS cells; however, under growing conditions the antisense oligonucleotide did not affect cell growth. Furthermore, the antisense oligonucleotide suppressed the antiproliferative effect of vesnarinone. These results suggest that TSC-22 may be a negative growth regulator and may play an important role in the antiproliferative effect of vesnarinone.

DOI： 10.1038/bjc.1998.11

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer and metastatic cancer respectively. We examined the expression of matrix-degrading enzymes and their inhibitors in these cell lines. Both cell lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9, membrane-type MMP and urokinase-type plasminogen activator. In addition to these enzymes, BHY cells secreted proMMP7 and procathepsin L, while HN cells secreted a large amount of active MMP2. BHY cells secreted a tissue inhibitor of matrix metalloproteinase, TIMP2, but only a trace level of TIMP1. Contrary to BHY cells, HN cells secreted TIMP1, but only a trace level of TIMP2. When we inoculated these cells into the masseter muscle of nude mice, both types of cell formed solid tumors, whose microscopic appearance was identical to that of the original tumors. BHY tumors were highly differentiated squamous-cell carcinomas, and invasive to the masseter muscle and the mandibular bone. Despite their local aggressiveness, BHY tumors did not metastasize to any distant organs. HN tumors were poorly differentiated squamous-cell carcinomas, weakly invasive to the muscle, but not to the mandibular bone. However, HN tumors frequently metastasized to cervical lymph nodes, These results suggest that the net activity of MMP2. (active MMP2/TIMP2) and cathepsin L secreted from cancer cells may contribute respectively to lymph-node metastasis and to bone invasion by oral cancer cells. (C) 1997 Wiley-Liss, Inc.

DOI： 10.1002/(sici)1097-0215(19970106)70:1<120::aid-ijc18>3.0.co;2-p

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PubMed

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掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/cancers16081492

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掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/cancers16081492

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：英語  

Nonocclusive mesenteric ischemia (NOMI) causes mesenteric ischemia and intestinal necrosis despite the absence of organic obstruction, such as thrombi and emboli in mesenteric blood vessels, and it has an extremely poor prognosis. We report a case of NOMI developed during bioradiotherapy (BRT) with cetuximab for cervical lymph node metastasis of tongue cancer. The patient was a 73-year-old man who underwent right radical neck dissection for neck lymph node metastasis after tongue cancer surgery. Postoperatively, the patient received BRT with cetuximab. On the 34th day after BRT, the patient had abdominal distension and a decreased level of consciousness. Contrast-enhanced computed tomography revealed mesenteric ischemia without thrombi and extensive intestinal emphysema. The patient was diagnosed with NOMI. Furthermore, he had septic shock and was treated with vasopressors and antibacterial agents; however, the condition of the patient did not improve, and he died on the same day.

DOI： 10.7759/cureus.57229

PubMed

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

悪性腫瘍はゲノム・エピゲノム異常により生じることが知られている。口腔扁平上皮癌(OSCC)も例外ではなく、種々の遺伝子異常が国内外で多数報告されている。近年、がん組織や血液を用いた種々のがん遺伝子パネル検査が保険収載され、抗がん薬選択の科学的根拠として用いられる「ゲノム医療」が行われている。しかし、その対象は標準治療に抵抗性を示す固形がんや、標準治療が確立されていない希少がんに限られており、OSCCの一次治療前にゲノム・エピゲノム異常を検査し把握することはできない。今回、OSCCのゲノム・エピゲノム異常についてこれまでの知見を概説するとともに、ゲノム・エピゲノム異常の頸部リンパ節転移予測への活用、およびリキッドバイオプシーへの活用に向けた研究について概説した。

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Introduction The prognosis of oral squamous cell carcinoma (OSCC) is often poor despite standard treatments. Additionally, no useful prognostic markers are available. Therefore, we aimed to investigate the relationship between serum Interleukin-6 (IL-6) levels and prognosis and explore its local and systemic effects in patients with OSCC. Methods Ninety-five new cases of OSCC were included, and the prognosis was compared between high and low serum IL-6 groups. The localization of IL-6 in OSCC tissues was examined. Furthermore, a comprehensive gene expression analysis was performed in OSCC tissues and compared between the two groups. Results A significant difference in overall survival and disease-free survival was observed. Furthermore, a substantial expression of IL-6 was localized in the stroma. Comprehensive gene expression analysis of tumor localization showed increased expression of genes related to oxidoreductase and lipid metabolism in the primary tissues of the group with high serum IL-6 levels. Regarding the correlation between blood tests and serum IL-6 levels, a strong positive correlation was observed between inflammatory responses and nutritional factors. Conclusion These results suggest that serum IL-6 may be a prognostic factor for metabolic abnormalities in patients with OSCC and that aggressive nutritional interventions may contribute to prognosis.

DOI： 10.7759/cureus.54439

PubMed

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Pubtexto  

DOI： 10.36266/ijcrci/213

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

脊髄硬膜外膿瘍は脊柱管内硬膜外に膿瘍を形成し,脊髄および脊髄根障害を引き起こすまれな疾患である.今回われわれは,口腔内細菌が起因菌と考えられた頸椎硬膜外膿瘍の1例を経験したので報告する.患者は63歳男性.既往歴は心房細動,緑内障,副鼻腔炎であった.頸部痛,咽頭痛,発熱を認め,咽後膿瘍疑いで当院耳鼻咽喉科を受診し,同日緊急入院となった.入院時の造影CT検査では同部に明らかな膿瘍形成は認めなかったため,整形外科に対診の結果,当初は頸長筋炎が疑われた.その後,第4病日に左側第二大臼歯相当部の疼痛精査目的に,当科紹介受診となった.パノラマエックス線検査,造影CT検査では,左側第二大臼歯根尖に透過像を認め,左側上顎洞底に近接していた.両側上顎洞,篩骨洞および蝶形骨洞に不透過像の亢進を認め,左側第二大臼歯根尖性歯周炎,両側副鼻腔炎と診断した.第7病日から再度熱発を認め,血液検査で炎症反応の増悪を認めた.造影CT検査では左側後咽頭間隙の軟部組織陰影の増大および頸椎C2～4の左側硬膜外に膿瘍形成を認めた.発熱と後頸部痛を認めたが,神経根症状は認めず,病期はI期の左側頸椎硬膜外膿瘍と診断した.左側上顎第二大臼歯の根尖病巣から上顎洞炎,篩骨洞炎,蝶形骨洞炎と炎症が波及し,後咽頭間隙を経由して頸椎硬膜外膿瘍を形成した可能性が考えられた.口腔内細菌を標的とし,第10病日からsulbactam/ampicillin(SBT/ABPC)12g/dayを開始し,消炎を行った.第12病日に原因歯と考えられた左側第二大臼歯の抜歯を行った.抜歯時に根尖から採取した検体より,血液培養でも陽性であったStreptococcus intermediusが検出された.第31病日までSBT/ABPC投与を継続したところ,頸椎硬膜外膿瘍は消退し,両側副鼻腔炎も改善を認めた.本症例では神経症状が出現する前に頸椎硬膜外膿瘍と診断されたため,保存的加療で後遺障害なく治癒した.(著者抄録)

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：英語   出版者・発行元：愛媛医学会  

目的:術後放射線治療(PORT)を受けた高リスク頭頸部扁平上皮癌(HNSCC)患者において、複数の治療関連因子が臨床転帰に影響を与えることが知られている。今回、実臨床における高リスクHNSCC患者のPORT後における局所領域制御に影響する因子を検討した。方法:2010年5月から2020年4月に、PORTを受けた高リスクHNSCC患者58名を対象とした。切除断端陽性、被膜外進展を伴うリンパ節転移(ECE)、多発性リンパ節転移を有する患者に対してPORTを施行した。PORTの線量は中央値60Gy(50～66Gy)であった。36名の患者が同時化学療法/分子標的療法を受けた。追跡期間中央値は28ヵ月(5～109ヵ月)であった。結果:2年全生存率、無増悪生存率、局所領域制御率は、それぞれ80%、62%、81%であった。患者関連因子(年齢、性別、炎症マーカー値)のみで、局所領域制御率に有意な差が認められ、治療関連因子と腫瘍関連因子(原発腫瘍部位、リンパ管/脈管浸潤、神経周囲浸潤、断端陽性、ECEを伴うリンパ節転移、多発性リンパ節転移)では、局所領域制御率に有意差は認められなかった。結論:積極的に治療関連因子の改善を試みた結果、PORTを受けた高リスクHNSCC患者の局所領域制御には患者関連因子の影響が強く残った。(著者抄録)

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.ajoms.2021.10.011

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：日本語   出版者・発行元：中・四国矯正歯科学会  

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記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：日本語   出版者・発行元：(一社)日本口蓋裂学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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担当区分：筆頭著者,　責任著者  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔腫瘍学会  

近年の治療法の向上にもかかわらず、約30年の間、早期口腔癌の治療成績は改善を認めていない。早期口腔癌における頸部リンパ節転移の有無は最も重要な予後因子である。それゆえに、頸部リンパ節転移の早期発見は予後の改善につながると考えられる。cN0症例における頸部マネージメントについては、経過観察もしくは予防的頸部郭清術のいずれかが選択されてきており、長年の論点となってきた。センチネルリンパ節生検は多くの領域で受け入れられている方法であり、口腔癌においては頸部郭清術の適否を判断する検査法である。本研究ではセンチネルリンパ節生検症例の同定部位、転移陽性レベル等から予防的頸部郭清術の適否について検討を行うとともに、今後の展望について述べる。(著者抄録)

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

薬剤関連顎骨壊死のポジションペーパーの中で、ビスフォスフォネート製剤(Bisphosphonates:BPs)の休薬が顎骨壊死発症を予防するか否かの明確な指標は示していない。本研究では、骨粗鬆症患者に対して、抜歯に際しBPs休薬後、骨代謝マーカーである尿中CTX値、血中P1NP値の変動を測定し、休薬による骨代謝の回復状態の検討を行った。BPs内服患者の抜歯時に、BPs処方医に休薬が可能であるかを確認の上、休薬を依頼した。その結果、6ヵ月のBPs休薬が可能であった骨粗鬆患者のうち、継続して骨代謝マーカーの測定ができた患者は21名であった。それらの患者は当科の顎骨壊死発症予防プロトコールに従って2011年から2014年の間に抜歯を行った。対象患者において、尿中CTX値、血中P1NP値を、休薬前、抜歯直前(概ね休薬3ヵ月後)、抜歯後3ヵ月(概ね休薬6ヵ月後)に測定した。尿中CTX値は上昇傾向であったが有意水準には達していなかった(3ヵ月目:p=0.36、6ヵ月目:p=0.20)。血中P1NP値は休薬3ヵ月目で上昇傾向が認められ(p=0.17)、6ヵ月目では有意な上昇が認められた(p=0.01)。以上の結果より、3ヵ月あるいは6ヵ月のBPs休薬により骨粗鬆症患者において、少なくとも静的な状態での骨代謝は回復していることが示唆された。(著者抄録)

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記述言語：日本語   出版者・発行元：獨協医学会  

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

当科における感染性心内膜炎(IE)発症リスク患者の歯科処置内容について検討し、当院心臓・血管内科でのIE発症患者の血液培養から検出された菌種の変遷について検討した。歯科処置を実施したのは967症例(男性567例、女性400例、平均67.5歳)であった。処置内容は抜歯(927例)、スケーリング(801例)、抜糸(427例)の順で多く認められた。これらの処置に対し、経過が追跡できたすべての症例において、歯科処置後に明らかなIEの徴候は認めなかった。血液培養から検出された菌種について検討したのは76例であり、口腔内常在菌の代表菌種であるviridans group streptococciが前期(2000年1月〜2010年12月)では31例中17例(54.8%)、後期(2011年1月〜2017年3月)では45例中17例(37.8%)に検出された。当院でIEと診断された患者で、血中から口腔内常在菌種が検出された症例は前期17/31(54.8%)に対し、後期17/45(37.8%)であった。またStaphylococcus aureusは前期4例に対し、後期は11例から検出された。

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

当科口腔ケア外来を受診した心移植予定患者6例(男性3例、女性3例、平均41.2歳)の口腔機能管理に関して検討した。原疾患はいずれも拡張型心筋症で、NYHAはII度が2例、III度が3例、IV度が1例であった。症例1、3、4は初診時には植え込み型LVADが装着されており、症例2、5は当院入院中に装着され、症例6は最終的に装着されなかった。症例3は移植待機3年目に、症例4は移植待機5年目に、それぞれ他院にて心移植が施行された。症例1、症例2、症例5は現在も移植待機中であり、継続的に口腔ケアを行っている。症例6は移植希望登録前に患者家族からのサポートが得られず移植は断念したが、重症心臓疾患患者であるため、継続的に口腔ケアを行っている。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

口腔癌に対する手術回避症例に対してResponse Evaluation Criteria in Solid Tumors(RECIST)評価を行い、治療成績について検討した。当科で経験した口腔癌症例311例中、根治手術を回避した13例(男性8例、女性5例、平均69.4歳)を対象とした。病期と治療完遂率については、全13例中9例が治療を完遂している。局所進行例の内訳はそれぞれ口峡から下咽頭への浸潤と鎖骨下静脈への浸潤を認め、切除不能と判定された。治療方法については化学放射線療法、放射線療法、放射線併用選択的動注化学療法、分子標的薬併用動注化学療法を行った。腫瘍の変化量は13例中10例で病変の消失を認めており、当科での根治的手術を回避した口腔癌の治療成績はおおむね良好であると考えられた。10例中4例で完全奏効(CR)判定が得られた。CR判定に至らなかった症例については、いずれも画像上での腫瘍縮小効果は認めるものの非標的病変を伴っており、腫瘍マーカーは基準範囲上限値を上回り部分奏効(PR)または非奏効/非進行(nonCR/nonPD)の判定となった。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

口腔癌手術患者の周術期における体組成を高精度体成分分析装置(InBody)で測定し、血液検査と併せて栄養管理、水分管理の評価を行った。当科にて口腔癌の手術を行い、術後1週間以内のInBody測定日に離床可能であった15例(男性8例、女性7例、平均74.3歳)を対象とした。基礎代謝量と水分量は全例で術前の計測からほぼ一定の値で推移しており、低下は認めなかった。体重とBMIは全例で入院時から緩やかに低下を認めた。体脂肪率と筋肉量は全例で術前の計測からほぼ一定の値で推移しており、低下は認めなかった。総蛋白(TP)は術前から基準値以下であった4例を含めた13例で術直後の測定において低下を認めたが、徐々に回復し経口摂取を開始する術後約10日で術前と同程度まで回復した。アルブミンは術前から基準値以下であった8例を含めて全例で術直後の測定において低下を認めたが、TPと同様に術後約10日で術前と同程度まで回復した。

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記述言語：日本語   出版者・発行元：(一社)日本歯科薬物療法学会  

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記述言語：日本語   出版者・発行元：(一社)日本歯科薬物療法学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：日本語   出版者・発行元：獨協医学会  

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その他リンク： https://search.jamas.or.jp/default/link?pub_year=2018&ichushi_jid=J04516&link_issn=&doc_id=20180921390022&doc_link_id=http%3A%2F%2Fid.nii.ac.jp%2F1199%2F00002114%2F&url=http%3A%2F%2Fid.nii.ac.jp%2F1199%2F00002114%2F&type=%E7%8D%A8%E5%8D%94%E5%8C%BB%E7%A7%91%E5%A4%A7%E5%AD%A6%EF%BC%9A%E7%8D%A8%E5%8D%94%E5%8C%BB%E7%A7%91%E5%A4%A7%E5%AD%A6%E3%83%AA%E3%83%9D%E3%82%B8%E3%83%88%E3%83%AA&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F80252_3.gif

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Dokkyo University School of Medicine  

We conducted a clinical study of 75 odontogenic tumors retrieved from the medical charts of our department from 2003 to 2012. The odontogenic tumors were reclassified according to the revised WHO classification (2005). The tumors consisted of 22 keratocystic odontogenic tumors, 16 ameloblastomas, 16 odontomas, 13 cases of osseous dysplasia, 3 cases of fibrous dysplasia, 2 calcifying cystic odontogenic tumors, 1 odontogenic myxoma, 1odontogenic fibroma,and adenomatoid odontogenic tumor. The male to female ratio of all odontogenic tumors was 1.5: 1. The average age of the patients at operation was 39.8 years. Keratocystic odontogenic tumors and ameloblastomas occurred frequently in the molar region of the mandible, but odontomas occurred frequently at anterior sites of the maxilla. The treatment modalities for the keratocystic odontogenic tumors were as follows: extirpation and primary closure (7 cases), extirpation with the wound left open (7 cases), biopsy followed by fenestration (5 cases), and fenestration followed by extirpation (3 cases). The treatment modalities for the ameloblastomas were as follows: extirpation with the wound left open (8 cases), segmental resection of the mandible (3 cases), marginal mandibulectomy (3 cases), biopsy followed by fenestration (1 case), and fenestration followed by extirpation (1 case). All of the odontomas (16 cases) were extirpated. Seventyone of 75 cases (94.7％) did not show recurrence. However, one case of ameloblastoma, one case of keratocystic odontogenic tumor, and one case of myxoma recurred after initial treatment. Moreover, one case of ameloblastoma that was treated previously at another hospital also recurred.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

Carbonated apatite (CO(3)Ap) is the inorganic component of bone. We have proposed a new method for the fabrication of CO(3)Ap blocks based on a dissolution-precipitation method using a synthetic precursor. The aim of this study is to examine the effects of low crystalline CO(3)Ap on initial cell attachment, proliferation and osteoblastic differentiation of human bone marrow cells (hBMCs) using sintered hydroxyapatite and tissue culture plates as controls. Initial cell attachment and proliferation were assessed with a MTT assay. Expression of osteoblastic markers was examined by reverse transcription-polymerase chain reaction. XRD and FT-IR results showed formation of B-type carbonate apatite with lower crystallinity. No difference was observed for initial cell attachment between HAp and CO(3)Ap discs. hBMSC attached more significantly on tissue culture plate than on HAp and CO(3)Ap discs. The number of cells on HAp was higher than that on CO(3)Ap until day 7, after which the number of cells was similar. hBMSC proliferated more significantly on tissue culture plate than on HAp and CO(3)Ap discs. In contrast, hBMCs incubated on CO(3)Ap demonstrated much higher expression of osteoblastic markers of differentiation, such as type I collagen, alkaline phosphatase, osteopontin and osteocalcin, than hBMCs on HAp. On the tissue culture plate, they were not any change throughout the culture period. These results demonstrated that low crystalline CO(3)Ap exhibit higher osteoinductivity than HAp.

DOI： 10.1007/s10856-015-5431-5

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPANDIDOS PUBL LTD  

Salivary gland cancer (SGC) has a comparatively poor prognosis and is prone to frequent recurrence and metastases. Therefore, the development of more effective chemotherapy against SGC is desirable. The aim of the present study was to investigate the antitumour effects of valproic acid (VPA) against SGC in vitro and in vivo. Two human SGC cell lines (HSY and HSG cells) were used in the present study. The effects of VPA on the proliferation of SGC cells in vitro were assessed by MTT assay. Cancer cells treated with VPA were subjected to cell cycle analysis by flow cytometry. In addition, the expression levels of p21 and p27 were examined by real-time RT-PCR to identify the mechanisms of the antitumour effect of VPA on SGC. The effects of VPA on cancer growth in vivo were evaluated in a xenograft model. VPA inhibited the proliferation of SGC cells in a dose-dependent manner in vitro. Degenerated cancer cells were observed at high concentrations of VPA. In the cell cycle analysis, VPA induced cell-growth inhibition and G1 arrest of cell cycle progression in both cancer cell lines in a time- and dose-dependent manner. VPA markedly upregulated the mRNA expression levels of both p21 and p27 in both SGC cell lines in a time-dependent manner. In the xenograft model experiment, VPA treatment markedly inhibited the growth of salivary gland tumours when compared with the growth of the untreated controls. VPA may be a valuable drug in the development of better therapeutic regimens for SGC.

DOI： 10.3892/or.2013.2959

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Hindawi Publishing Corporation  

Aim. Tonsilloliths are calcified structures that develop in tonsillar crypts. They are commonly detected in daily clinical practice. The prevalence of tonsilloliths was 16 to 24% in previous reports, but it is inconsistent with clinical experience. The aim of this study is to clarify the prevalence, number, and size distribution of tonsilloliths using computed tomography (CT) in a relatively large number of patients. Materials and Methods. We retrospectively reviewed the scans of 2,873 patients referred for CT examinations with regard to tonsilloliths. Results. Palatine tonsilloliths were found in 1,145 out of 2,873 patients (39.9%). The prevalence of tonsilloliths increased with age, and most commonly in patients of ages 50-69. The prevalence in the 30s and younger was statistically lower than in the 40s and older (P &lt
0.05). The number of tonsilloliths per palatine tonsil ranged from one to 18. The size of the tonsilloliths ranged from 1 to 10 mm. For the patients with multiple CT examinations,the number of tonsilloliths increased in 51 (3.9%) and decreased in 84 (6.5%) of the tonsils. Conclusions. As palatine tonsilloliths are common conditions, screenings for tonsilloliths during the diagnosis of soft tissue calcifications should be included in routine diagnostic imaging.

DOI： 10.1155/2014/940960

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3892/or.2013.2480

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3892/ijo.2013.1786

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPANDIDOS PUBL LTD  

Docetaxel (DOC) and 5-fluorouracil (5-FU) are important anticancer agents widely used in the treatment of a variety of cancers including oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the antitumor efficacy of the sequential administration of DOC and 5-FU against OSCC cells (B88 and CAL27 cells) in vitro and in vivo. In in vitro growth inhibition assays, sequential treatment with DOC followed by 5-FU was more effective in inhibiting cancer cell growth than 5-FU followed by DOC, single treatment with DOC or 5-FU, or combined treatment with DOC and 5-FU. Furthermore, DOC followed by 5-FU significantly inhibited tumor growth in vivo compared to 5-FU followed by DOC. To understand the mechanisms underlying the enhanced growth inhibitory effect of the administration sequence, DOC followed by 5-FU, we examined the expression of 5-FU metabolic enzymes such as thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD) and orotate phosphoribosyl transferase (OPRT), which were known to regulate the antitumor effect of 5-FU, by real-time RT-PCR and western blot analysis. Downregulation of TS and DPD expression and upregulation of OPRT expression were induced by DOC treatment, suggesting that DOC enhanced the efficacy of 5-FU by altering the expression of its metabolic enzymes. These results indicate that sequential treatment with DOC followed by 5-FU could be a promising therapeutic strategy for oral cancer.

DOI： 10.3892/ijo.2012.1544

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

The purpose of this study was to evaluate the effectiveness and adverse events of combination chemotherapy with oral S-1 administration following docetaxel (DOC) treatment by superselective intra-arterial infusion as neo-adjuvant chemotherapy (NAC) for patients with oral squamous cell carcinoma. Thirteen patients were enrolled in this study (9 men and 4 women, with a mean age of 61. 0 years). All patients were given S-1 65mg/m(2) per day for 14 days, and DOC 40-50mg/m(2) by intraarterial infusion was administered. The locoregional response evaluated 3 weeks after administration was 100%, including a 69. 2% complete response. According to Oboshi and Shimosato's classification, histological evaluation of surgical specimens revealed that 3 cases were Grade II a, 4 cases Grade II b, 1 case Grade IV a, and 4 cases Grade IV c. The severe side effects were neutropenia and cerebral infarction. The present study suggests that combination chemotherapy with S-1 and DOC by superselective intra-arterial infusion would be an effective and safe regimen in NAC for oral squamous cell carcinomas.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPANDIDOS PUBL LTD  

Hypoxia promotes the invasive and metastatic potential of tumour cells. A recent study has shown that the activation of the chemokine receptor CXCR4 by lack of oxygen in breast cancer is HIF-1-dependent. We have previously demonstrated that CXCR4 signalling is involved in the establishment of lymph node metastasis in oral squamous cell carcinoma (OSCC). In this study, we investigated a correlation between CXCR4 and HIF-1 alpha expression in OSCC. Immunohistochemistry showed that CXCR4 was expressed in 20 of 85 OSCC tissues, while HIF-1 alpha was expressed in 51 of 85 samples. There was a significant correlation between the expression of CXCR4 and HIF-1 alpha. In human OSCC cells, hypoxia markedly enhanced the expression of both HIF-1 alpha and CXCR4. Furthermore. synthetic small interfering RNA specific for HIF-1 alpha significantly suppressed the expression of this protein, and also attenuated the induction of CXCR4 expression under hypoxic conditions. These results indicated that HIF-1 alpha regulated hypoxia-induced CXCR4 expression in OSCC.

DOI： 10.3892/or_00000275

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor superfamily of ligand-activated transcription factors. Recently, we have demonstrated that PPARγ is expressed in human salivary gland tumors and its ligands inhibit the growth of cultured salivary gland cancer cells. However, expression and function of PPARγ in normal and neoplastic human oral squamous epithelium remains unclear. In the present study, we examined PPARγ expression in human oral squamous cell carcinoma (OSCC) and tested its ligands for any antitumor effect. PPARγ mRNA was detected by RT-PCR in some OSCC tissues and cultured cells, but the PPARγ protein showed neither expression nor ligand-induced transcriptional activity. Despite loss of PPARγ function, synthetic PPARγ ligands caused significant dose-dependent inhibition of cancer cell growth. These results suggest that PPARγ function is inactivated in OSCC cells and the anti-proliferative effect of its synthetic ligands is independent of PPARγ. © 2003 Elsevier Ltd. All rights reserved.

DOI： 10.1016/S1368-8375(03)00108-8

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記述言語：英語   出版者・発行元：LIPPINCOTT WILLIAMS & WILKINS  

Vesnarinone has been shown to be a unique anti-proliferating, differentiation-inducing and apoptosis-inducing drug against several human malignancies, including leukemia and several solid tumors. Furthermore, vesnarinone potentiates the effect of conventional cytotoxic chemotherapy or radiation therapy. Combination of differentiation-inducing therapy by vesnarinone with conventional chemotherapy or radiation therapy might be second- or third-line therapy in patients with advanced cancer. Analysis of the molecular mechanisms of the tumor differentiation therapy by vesnarinone might provide selective and targeted molecules for novel tumor dormancy therapy.

DOI： 10.1097/01.cad.0000078735.65608.bf

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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J-GLOBAL

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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記述言語：英語   出版者・発行元：(一社)日本癌学会  

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J-GLOBAL

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

J-GLOBAL

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記述言語：日本語  

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記述言語：日本語   出版者・発行元：(一社)日本歯科薬物療法学会  

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記述言語：日本語   出版者・発行元：(一社)日本歯科薬物療法学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

62歳女性。左側上顎骨骨肉腫T1N0M0 stage IIAに対し、術前AI療法3回、腫瘍切除と術後にAI療法2回を行ったが、術後化学療法開始後172日目に急性リンパ性白血病を発症した。Hyper CVAD療法は効果がなく、初診より516日目(術後284日目、術後化学療法191日目)に敗血症性ショックで死亡した。経過観察中に原発巣の再発や明らかな遠隔転移は認めかった。なお、骨肉腫に対する化学療法後に発症した二次性白血病の報告は国内外で12例(国内1例)であった。

DOI： 10.5794/jjoms.65.51

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：栃木県歯科医師会  

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その他リンク： http://search.jamas.or.jp/link/ui/2020039618

記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本神経治療学会  

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記述言語：日本語  

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記述言語：日本語  

DOI： 10.5794/jjoms.64.568

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

口腔癌患者に対してFDG-PET/造影CT検査を施行し、その感度・特異度・正診率について検討した。対象は、2005年4月から2013年3月までの8年間に当科で根治的手術療法を施行した口腔扁平上皮癌一次症例患者115例で、性別は男性70例、女性45例、平均年齢は63.0歳であった。原発部位は、舌66例、下顎歯肉27例、上顎歯肉10例、口底6例、頬粘膜6例であった。術前臨床診断は、T分類ではT1:12例、T2:50例、T3:11例、T4:42例、N分類ではN0:64例、N1:22例、N2:29例で遠隔転移症例はみられなかった。Stage分類は、Stage I:10例、II:35例、III:17例、IV:53例であった。FDGの集積を認めたリンパ節のSUVmaxを計測し、カットオフ値を変化させた時のFDG-PET/造影CTにおける頸部リンパ節職転移の診断精度について検討した。肉眼でFDGの異常集積が確認できたリンパ節のSUVmaxの最低値である2.0をカットオフ値と設定した場合、症例ごとの検討では、感度84.9%、特異度95.2%、正診率90.4%であった。一方、リンパ節ごとの診断精度の検討では、感度67.6%、特異度99.5%、正診率98.0%であった。カットオフ値を上昇させるにつれ、感度は低下傾向、特異度は上昇傾向を示した。FDG-PET/造影CTにMRI、触診それぞれの所見を加味した際の診断精度は、感度93.6%、特異度94.5%、正診率94.1%であった。また、FDG-PET/造影CTにおいて偽陽性であった症例は3例であった。一方、偽陰性症例は8例で、原発部位は舌7例、下顎歯肉1例であった。

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J01073&link_issn=&doc_id=20181031470001&doc_link_id=130007535674&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F130007535674&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

記述言語：日本語   出版者・発行元：日本口腔顎顔面外傷学会  

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記述言語：日本語   出版者・発行元：(株)北隆館  

ビスフォスフォネート製剤による顎骨壊死(BRONJ)は増加傾向にあり、近年ではデノスマブなどの抗RANKL抗体や血管新生阻害薬などの分子標的薬でも同様の顎骨壊死を生じることが報告されている。米国口腔顎顔面外科学会は、これら一連の顎骨壊死に薬剤関連顎骨壊死(MRONJ)の名称を用いることを提唱している。MRONJは発症の頻度は低いが、骨吸収抑制薬投与により一定の確率で起こりうる有害事象であり、その誘因として、抜歯などの侵襲的歯科口腔外科治療や、不良な口腔環境が指摘されている。本稿では、MRONJの実態を原因、誘因、治療、予防の観点から一部私見を交え概説する。(著者抄録)

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記述言語：英語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：獨協医学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

骨性異形成症(OD)は、経過に伴い多様な組織像を呈する。臨床上は無症状に経過し、初期の病変では線維性結合組織の増生を主体として放射線学的に透過像を呈するが、経過とともにセメント質様または骨様の硬組織形成を伴い放射線学的に不透過像が混在するようになり、最終的には病変の中心部が放射線学的に不透過性病巣となる。今回、透過性病変と不透過性病変が同時に存在した下顎骨OD患者を10年以上経過観察できた症例を経験した。症例は初診時46歳の女性で、OD病変の前方部にX線不透過像を認め、後方部に嚢胞様骨空洞を伴うX線透過像を認めた。前方部の病変は容易にODと診断できたが、後方部については、空洞を呈した病変をODの一部と診断できるか否かが問題となった。生検による病理組織像では、後方部病変の境界において骨壁の周囲に線維性組織の増生を認めており、OD病変の一部と考えられた。本例の嚢胞様骨空洞部は顎骨の膨隆を伴い、いわゆる単純性骨嚢胞とは特徴が異なっており、発生機序として、一度充実性の病変が形成された後に何らかの原因で内部吸収が起こり生じたと考えられた。

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

口臭防止が期待でき口腔衛生の維持に貢献すると考えられる口臭防止剤ハイザックNリンスについて臨床的検討を行った。口腔ケア外来を受診した周術期口腔機能管理を必要とする手術予定の患者22例を対象とした。細菌カウンタによる細菌数の検査では両群(ハイザック群、対照群)に有意な差はなく、術後においてハイザック群でもわずかに減少傾向であるが有意差は認められなかった。口腔水分計ムーカスによる口腔湿潤度の検査では、わずかに増減があるが有意差はみられず、口臭測定組織ブレストロンIIによる口臭検査では、術前と術後で対照群とハイザック群でppdの値に平均値の差がみられた。また、主観的評価では口臭は両群とも減少傾向を示したが、ハイザック群のみ有意差を認めた。口腔乾燥は術前では両群に差は認めないが、両群とも減少傾向を示し、ハイザック群のみ有意差を認めた。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

口腔ケアの取り組みの効果判定のひとつとして、当院で実施された血液培養の結果の変遷に着目し検討を行った。2006年1月から2015年6月までの9年5ヵ月間に当院25科で実施された血液培養66277症例のうち、陽性となった10413症例を対象とした。血液培養陽性数は、検体数の増加とは比例することはなく、年平均約16%前後の陽性率で推移していた。検出きれた菌グループの割合は、腸内細菌が25%、非発酵菌が8%、グラム陽性球菌が49%、いわゆる嫌気性菌が3%、真菌が4%、その他が11%であった。また、口腔ケア外来初診患者数と口腔ケアリンクナース認定者数は、経年的に増加傾向を示していた。グラム陽性球菌の検出率と口腔ケア外来初診患者数、リンクナース数は高い負の相関を示した。

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記述言語：日本語   出版者・発行元：(一社)日本口蓋裂学会  

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

潜在的悪性疾患群(OPMDs)と診断した病変のうち、悪性転化をきたした症例について臨床統計を行った。口腔粘膜疾患患者18611例のうち、初診時または初診時以降早期に病理組織検査によりOPMDsと診断された患者696名を対象とした。疾患の内訳は白板症225例、紅板症9例、扁平苔癬462例で、そのうち悪性転化が認められたものは白板症3例(1.33%)、紅板症2例(22.2%)、扁平苔癬7例(1.52%)であった。扁平苔癬罹患者のうち10年間経過観察可能であり、かつ悪性転化していない症例(非悪性転化症例)10例と悪性転化症例7例を比較した。扁平苔癬診断時の異形成の有無は悪性転化症例で14.3%であり、ほとんどの症例で異形成は認められなかった。非悪性転化症例では半数が臨床的に診断されており、生検を行っていた症例は半数であったが、生検を行った症例には全例で異形成を認めなかった。

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(株)デンタルダイヤモンド社  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

ビスフォスフォネート関連顎骨壊死(BRONJ)と診断した57例(男性17例、女性40例、平均年72.2歳)を対象とした。保存療法のみ30例、保存療法を施行したが無効であったため外科療法は27例であった。発症部位は下顎臼歯部31部位、上顎臼歯部16部位と下顎前歯部12部位、上顎前歯部9部位であった。原疾患は、原発性骨粗鬆症17例、ステロイド誘発性骨粗鬆症15例、悪性腫瘍25例であった。57例中、保存療法が有効であった症例は18例であった。保存療法が無効であった39例中、外科療法を行わなかった症例は12例であった。保存療法が無効であった39例中、外科療法選択の判断基準を満たしたのは27例で、外科療法が有効であったのは25例であった。経口BP製剤で保存療法を施行した患者の有効率は36.4%、保存療法無効症例で外科療法を行った症例の有効率は100%、注射BP製剤で保存療法を施行した患者の有効率は25.0%、保存療法無効症例で外科療法を行った症例の有効率は75.0%であった。

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記述言語：日本語   出版者・発行元：獨協医学会  

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2017&ichushi_jid=J04516&link_issn=&doc_id=20180123420015&doc_link_id=http%3A%2F%2Fid.nii.ac.jp%2F1199%2F00001723%2F&url=http%3A%2F%2Fid.nii.ac.jp%2F1199%2F00001723%2F&type=%E0%D5%8B%A6%88%E3%89%C8%91%E5%8Aw%81F%E0%D5%8B%A6%88%E3%89%C8%91%E5%8Aw%83%8A%83%7C%83W%83g%83%8A&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F80252_3.gif

記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本口蓋裂学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔ケア学会  

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

口唇裂破裂の程度は、完全裂161例、不完全裂49例であった。一次症例における初診時年齢は生後1ヵ月以内に142例と全症例の50.0%が受診し、生後6ヵ月迄に全体の85.9%が受診した。出生時体重は、2500〜4000g未満の正常体重児が243例で、2500g未満の低出生体重児は54例であった。合併した先天異常は111例で、27例は唇顎口蓋裂、30例は口蓋裂、6例は口唇(顎)裂であった。合併した先天異常の内訳は、Robin sequenceが11例、羊膜索症候群が3例、13トリソミーが3例、口顔面指症候群が2例、22q11.2染色体欠損症候群が2例、それ以外はそれぞれ1例であった。口唇形成術は111例に三角弁法、口蓋形成術は、Push Back法が103例、Modified Push back法は51例であった。2歳迄に口蓋形成術を施行した症例における6歳時での評価では、鼻咽腔閉鎖機能不全なしが127例であった。言語成績は、6歳時には78例が正常構音を獲得した。矯正治療を開始したのは232例中73例であった。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

39年間に一次治療を行った口腔扁平上皮癌495例(男性307例、女性188例、17〜98歳)を対象とした。発生部位別では、舌が231例、下顎歯肉が96例、上顎歯肉が65例、頬粘膜が42例、口底が36例、口蓋・軟口蓋が11例、口唇が9例、その他が5例であった。UICCのTNM分類細で分類し、T2症例が196例と最も多く、N分類ではN0症例が283例と最も多かった。全症例で診断時に遠隔転移は認めなかった。UICCのStage分類で分類し、Stage 0が9例、Stage Iが74例、Stage IIが124例、Stage IIIが82例、Stage IVが206例であった。組織学的悪性度は、高分化型303例、中分化型118例、低分化型46例であった。治療方法の内訳は、手術単独146例、手術+化学療法160例、手術+放射線療法27例、手術+放射線化学療法75例、化学療法のみ10例、放射線療法のみ39例、放射線化学療法38例であった。病期別5年生存率は、Stage Iで94.6%、Stage IIで85.0%、Stage IIIで72.9%、Stage IVで62.6%であった。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

20年間に初診データベースに登録された55427名を対象とし、年度別外来初診患者数、疾患別患者数、受診経路、受診患者年齢の推移について検討した。年度別外来初診患者数は、1996年度から2004年度まで2000名程度で推移していたが、2007年度の口腔ケア外来開設に伴い、2011年度には4000名をこえた。一般歯科疾患1996年度は約700名であったが、1997年度から2005年度までは約700名から約900名で推移した。2007年度からは1000名を超え、2012年度以降は1500名を超えるようになった。炎症は1996年度で335名であり、その後200名から350名の間で推移してきたが、近年は250名前後を推移した。口腔粘膜疾患は1996年度約150人であったが、近年では350人前後まで増加した。顎関節疾患は患者数の大きな変動は認められなかったが、一般歯科疾患受診患者の増加に伴い、経年的な割合の減少を認めた。唾液腺疾患は経年的に増加し、近年は約150人まで増加した。1996年度では60歳以上の患者が20%程度であったが、2010年ころから40%程度になった。

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記述言語：日本語   出版者・発行元：(一社)日本外科学会  

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記述言語：日本語   出版者・発行元：獨協医学会  

2003年から2012年までの9年間に当科で診断・治療を行った歯原性腫瘍75症例について,2005年WHO分類に基づいて臨床的検討を行った.疾患別症例数は角化嚢胞性歯原性腫瘍が22例,エナメル上皮腫16例,歯牙腫16例,骨性異形性症13例,線維性異形性症3例,石灰化嚢胞性歯原性腫瘍2例,歯原性粘液腫,歯原性線維腫,腺様歯原性腫瘍が各1例であった.男女比は1.5対1であった.手術時年齢は,平均39.8歳であった.発生部位別症例数は,角化嚢胞性歯原性腫瘍,エナメル上皮腫は下顎大臼歯部に多く,歯牙腫は,上顎前歯部に多かった.治療方法は,角化嚢胞性歯原性腫瘍に対しては摘出後閉鎖7例,摘出後開放7例,生検後開窓5例,開窓後摘出3例であった.エナメル上皮腫は摘出後開放8例,区域切除3例,辺縁切除3例,開窓1例,開窓後摘出1例であった.歯牙腫16例に対しては摘出術を施行した.治療成績は75例中71例(94.7%)は再発を認めなかったが,一次症例で,エナメル上皮腫1例,角化嚢胞性歯原性腫瘍1例,歯原性粘液腫1例の3症例,他院で治療後のエナメル上皮腫1例に再発が認められた.(著者抄録)

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔診断学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

獨協医科大学病院は地域の基幹病院として、多くの有病者や障がい児(者)の歯科治療も行っている。そこで、獨協医科大学病院口腔外科における全身麻酔下に施行した歯科治療を行った障がい児(者)の検討を行った。対象は獨協医科大学病院口腔外科で2007年1月から2016年3月までに全身麻酔下に外科処置を含めた歯科治療を行った患児(者)45名とした。経年的な症例数の変化として2006年以前は、全身麻酔下の障がい児(者)歯科治療はなく、2007年2名、2008年2名、2009年1名、2010年6名、2011年9名、2012年7名、2013年7名、2014年5名、2015年4名、2016年2名であった。受診経路は地域医療センター19名(42.2%)、かかりつけ歯科16名(35.6%)、直接6名(13.3%)、当院小児科、精神科がそれぞれ2名(8.9%)であった。治療に際し、当院麻酔科に日帰り全身麻酔を依頼したが、術前のリスク評価が十分に行えない症例が多いこと、術後合併症のリスクの問題から、検討した結果、治療前日入院、治療、翌日退院の2泊3日で管理を行った。処置歯数は合計326歯あり、その内訳は、永久歯263歯(80.7%)、乳歯63歯(19.3%)であり、1回の全身麻酔下における治療本数の平均は7.2歯であった。術中、術後に合併症を起こした症例はなかった。地域との連携構築により、2010年から症例数が増加傾向にあった。今後、栃木県の障がい児(者)のための歯科治療のシステムをますます広げていきたいと考えている。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：千葉医学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2016-4117

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2016-1925

Web of Science

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

2003〜2014年に舌癌の手術を施行した患者のうち、再発なく術後1年以上経過している61例を対象とし、アンケートによる主観的機能評価と、医療者による客観的機能評価を行った。アンケートによる評価の項目は、嚥下機能、咀嚼機能、会話機能とした。嚥下機能の評価方法は、藤本らのMTFスコアに準じ、「経管栄養のみ」を1点、「経管栄養併用」を2点、「食事形態の工夫」を3点、「若干の制限」を4点、「制限なし」を5点とした。咀嚼機能の評価方法は、山本の咬度分類に準じ、咬度1・2を「不良」、咬度3・4を「やや良好」、咬度5・6を「良好」とした。嚥下機能評価の結果、1点が1例(2%)、3点が7例(11%)、4点が23例(38%)、5点が30例(49%)であった。咀嚼機能評価の結果、「不良」が3例(5%)、「やや良好」6例(10%)、「良好」52例(85%)であった。医療者による客観的評価の項目は、嚥下機能、会話機能とした。会話機能の評価方法は降矢の方法に準じ、評価しえた46例の結果は、軽度障害が32例(70%)、中等度障害10例(22%)、高度障害4例(9%)であった。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

当院口腔ケア外来が開設された2007年10月から2014年12月までに同外来を受診した4141例を対象とし、「同外来初診患者数の推移」「紹介科別患者数」「依頼内容別患者数」「周術期口腔ケアの依頼時期」「化学療法・放射線療法患者の口腔ケア依頼時期」「移植患者の口腔ケア依頼時期」について検討した。結果、初診患者数は、開設当初には月間20例前後で推移していたが、年々増加し、近年では月間60〜70例となった。紹介科別患者数は、第二外科(消化器疾患を対象)が最も多く33%、次いで血液腫瘍内科15%であった。依頼内容別患者数は、「周術期口腔ケア」が最も多く36%、次いで「化学療法や放射線療法に関する口腔ケア」22%、「ベッドサイド口腔ケア」14%の順であった。周術期口腔ケアの依頼時期は、「手術当日・術後」が12%、「手術前日〜10日前」が55%、「11日以上前」が33%であった。化学療法・放射線療法患者の依頼時期は、「治療中・治療後」が51%、「治療前日〜10日前」が30%、「11日以上前」が19%であった。移植患者の依頼時期は、「移植中・移植後」34%、「前日〜10日前」4%、「11日以上前」62%であった。

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記述言語：日本語   出版者・発行元：(一社)栃木県歯科医師会  

2003〜2014年に治療した唾液腺腫瘍40例を対象とし、「病理組織学的分類」「性別」「年齢」「受診経路」「発生部位」「自覚症状」「病悩期間」「治療法」「予後」について検討した。病理組織学的分類(WHO分類)は良性腫瘍が19例、悪性腫瘍が21例で、良性腫瘍の内訳は多形腺腫18例、ワルチン腫瘍1例、悪性腫瘍の内訳は腺様嚢胞癌13例、粘表皮癌5例、多形低悪性度腺癌2例、明細胞癌1例であった。性別は、良性腫瘍例の男女比が2.2:1、悪性腫瘍例の男女比が1.1:1であった。年齢は、良性腫瘍例では30〜50歳代が多く、悪性腫瘍例では60〜70歳代が多かった。発生部位は、良性腫瘍では口蓋・頬部・口唇の小唾液腺が多く13例、次いで耳下腺4例、顎下腺2例であった。悪性腫瘍では口蓋・頬部の小唾液腺が多く10例、次いで顎下腺7例、舌下腺2例、下顎骨2例であった。治療法は、良性腫瘍では全例が外科的切除、悪性腫瘍では手術療法10例、手術療法+化学療法+放射線療法8例、化学療法+放射線療法1例であった。予後は、良性腫瘍では無病生存18例、死亡1例、悪性腫瘍では無病生存15例、担癌生存3例、死亡3例であった。

J-GLOBAL

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記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

CiNii Books

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その他リンク： http://search.jamas.or.jp/link/ui/2016109944

記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本口腔内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：日本口腔顎顔面外傷学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2015-3059

Web of Science

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：獨協医学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2014-1993

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2014-3888

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2014-1446

Web of Science

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

上部構造装着後3年を経過したインプラント1291本を対象に、ショートインプラントの有用性について臨床的検討を行った。1291本のうち、長さ10mm未満のショートインプラントは113本で、表面性状は滑面42本、粗面71本であり、年齢、インプラントの長さ・表面性状・埋入部位・直径、初期固定、治癒期間によるインプラントの脱落率を比較した結果、ショートインプラント全体と10mm以上の長さのインプラントの脱落率に有意差は認めなかった。また、表面性状別にみると、滑面ショートインプラントの脱落率は長さ10mm以上の滑面インプラントや粗面のショートインプラントよりも有意に高かったが、粗面ショートインプラントの脱落率は、10mm以上のインプラントと差がなく、表面性状の改良によってショートインプラントの治療成績が改善していることが明らかとなった。

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：日本口腔顎顔面外傷学会  

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2013-4799

Web of Science

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(一社)日本有病者歯科医療学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

症例は46歳女性で、7ヵ月前より左側下顎歯肉の腫瘤を自覚し、増大傾向となった。左側下顎側切歯〜第一大臼歯部の舌側辺縁歯肉から口底にかけて25×16mmの境界明瞭な有茎性腫瘤を、X線で同部に軽度の骨吸収像を認め、エプーリスと臨床診断し腫瘤切除術を施行した。腫瘤基部の軽度吸収した歯槽骨をラウンドバーで一層切削し、左側下顎小臼歯は根面を十分に掻爬し保存した。術後2年6ヵ月経過し再発はない。病理組織所見で、粘膜上皮下に多数のエオジン好性の大型細胞が散在し、腫瘤中心部には骨形成が認められた。強拡大では粘膜上皮下に、線維芽細胞様の紡錘形細胞の増殖像に加え、十数個前後の核を有し大小不整形態を示す散在性の多核巨細胞を認め、一部に巨細胞による貪食像もみられた。腫瘤辺縁部には出血やヘモジデリンの沈着を、中央部には骨芽細胞に縁取られた大小不規則な塊状の骨形成を認め、周辺性巨細胞肉芽腫(巨細胞エプーリス)と病理診断した。

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2012-4954

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2012-5329

Web of Science

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本顎顔面インプラント学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本顎変形症学会  

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記述言語：日本語   出版者・発行元：(株)癌と化学療法社  

口腔扁平上皮癌患者に対し、術前化学療法(neo-adjuvant chemotherapy:NAC)としてtegafur・gimeracil・oteracil potassium配合剤(S-1)を併用したdocetaxel(DOC)の超選択的動注化学療法を施行し、その治療効果および有害事象を検討した。対象は、未治療の口腔扁平上皮癌患者で男性9例、女性4例の計13例、平均年齢は61.0歳であった。薬剤の投与法は、S-1 65mg/m2/dayを14日間連日経口投与した後、Seldinger法で40〜50mg/m2のDOCを腫瘍の栄養血管に超選択的に動注した。4週間後に臨床的治療効果および有害事象を判定し、さらに手術標本を用いて病理組織学的効果を判定した。臨床的治療効果では、CR9例、PR4例で奏効率は100%、CR率は69.2%であった。病理組織学的効果では、大星、下里らの分類で、Grade IIaが3例、Grade IIbが4例、Grade IVaが1例、Grade IVcが4例であった。grade 3以上の有害事象は白血球減少がgrade 3:3例、grade 4:6例で、grade 3の食欲不振、下痢、口内炎が各1例にみられた。また、超選択的動注化学療法の合併症として1例に脳梗塞が発症した。以上より、S-1を併用したDOCの超選択的動注化学療法は口腔扁平上皮癌に対して高い奏効率が期待でき、NACとして有用であるが、脳梗塞などのリスクを術前に十分評価することが重要と考えられた。(著者抄録)

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2011-201

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC CANCER RESEARCH  

DOI： 10.1158/1538-7445.AM2011-2440

Web of Science

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本口腔腫瘍学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(一社)日本頭頸部癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：四国歯学会  

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記述言語：日本語   出版者・発行元：日本口腔組織培養学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本口腔科学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：日本語   出版者・発行元：四国歯学会  

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記述言語：日本語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：(一社)日本癌治療学会  

J-GLOBAL

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(公社)日本口腔外科学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：英語   出版者・発行元：日本癌学会  

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記述言語：日本語   出版者・発行元：(株)癌と化学療法社  

68歳、男性。右側舌縁部から舌根部のT3N2bM0,Stage IVA舌癌(口腔扁平上皮癌)と診断された。2004年6月25日入院し、S-1を120mg/日にて4週投与2週休薬を1クールとして投与を開始、放射線治療(RT)は1.8Gy/日週5日、計61.2Gyにて照射し、S-1とRT同時併用療法を施行した。2クール終了時の生検では腫瘍細胞を認めず、さらにCTにて右側頸部リンパ節転移巣の消失がみられた。その後UFTを300mg/日で継続投与し、外来にて経過観察中であるが治療開始後27ヵ月現在、再発兆候を認めていない。(著者抄録)

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その他リンク： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2007&ichushi_jid=J00296&link_issn=&doc_id=20070518370013&doc_link_id=%2Fab8gtkrc%2F2007%2F003405%2F013%2F0745-0747%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fab8gtkrc%2F2007%2F003405%2F013%2F0745-0747%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif