Updated on 2025/03/27

写真a

 
Kato Hirotaka
 
Organization
Graduate School of Science and Engineering (Science) Major of Science and Engineering Chemistry Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree

  • 博士（生命科学） ( 京都大学 )

  • 修士（生命科学） ( 京都大学 )

Research Areas

  • Life Science / Plant molecular biology and physiology

Research Subject

  • Molecular mechanisms for land plant development and its evolution

Education

  • Kyoto University   Graduate School of Biostudies

    - 2015.5

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Research History

  • Kobe University   Graduate School of Science Division of Biology

    2021.4 - 2022.2

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  • Kobe University   Graduate School of Science   Research Associate

    2020.3 - 2021.3

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  • Kobe University   Graduate School of Science   Research fellow

    2019.4 - 2020.3

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  • Wageningen University   Laboratory of Biochemistry   Postdoctoral researcher

    2015.10 - 2019.3

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Professional Memberships

  • THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

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  • THE BOTANICAL SOCIETY OF JAPAN

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Papers

  • Design principles of a minimal auxin response system Reviewed International journal

    Hirotaka Kato, Sumanth K. Mutte, Hidemasa Suzuki, Isidro Crespo, Shubhajit Das, Tatyana Radoeva, Mattia Fontana, Yoshihiro Yoshitake, Emi Hainiwa, Willy van den Berg, Simon Lindhoud, Kimitsune Ishizaki, Johannes Hohlbein, Jan Willem Borst, D. Roeland Boer, Ryuichi Nishihama, Takayuki Kohchi, Dolf Weijers

    Nature Plants   6 ( 5 )   473 - 482   2020.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Auxin controls numerous growth processes in land plants through a gene expression system that modulates ARF transcription factor activity1-3. Gene duplications in families encoding auxin response components have generated tremendous complexity in most land plants, and neofunctionalization enabled various unique response outputs during development1,3,4. However, it is unclear what fundamental biochemical principles underlie this complex response system. By studying the minimal system in Marchantia polymorpha, we derive an intuitive and simple model where a single auxin-dependent A-ARF activates gene expression. It is antagonized by an auxin-independent B-ARF that represses common target genes. The expression patterns of both ARF proteins define developmental zones where auxin response is permitted, quantitatively tuned or prevented. This fundamental design probably represents the ancestral system and formed the basis for inflated, complex systems.

    DOI： 10.1038/s41477-020-0662-y

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    Other Link： http://www.nature.com/articles/s41477-020-0662-y

  • Origin and evolution of the nuclear auxin response system Reviewed

    Sumanth K Mutte, Hirotaka Kato, Carl Rothfels, Michael Melkonian, Gane Ka-Shu Wong, Dolf Weijers

    eLife   7   2018.3

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：{eLife} Sciences Organisation, Ltd.  

    DOI： 10.7554/eLife.33399

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  • The Roles of the Sole Activator-Type Auxin Response Factor in Pattern Formation of Marchantia polymorpha Reviewed

    Hirotaka Kato, Masaru Kouno, Mayuko Takeda, Hidemasa Suzuki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi

    PLANT AND CELL PHYSIOLOGY   58 ( 10 )   1642 - 1651   2017.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

    Cell division patterning is important to determine body shape in plants. Nuclear auxin signaling mediated by AUXIN RESPONSE FACTOR (ARF) transcription factors affects plant growth and development through regulation of cell division, elongation and differentiation. The evolutionary origin of the ARF-mediated pathway dates back to at least the common ancestor of bryophytes and other land plants. The liverwort Marchantia polymorpha has three phylogenetically distinct ARFs: MpARF1, the sole 'activator' ARF; and MpARF2 and MpARF3, two 'repressor' ARFs. Genetic screens for auxin-resistant mutants revealed that loss of MpARF1 function conferred auxin insensitivity. Mparf1 mutants showed reduced auxin-inducible gene expression and various developmental defects, including thallus twisting and gemma malformation. We further investigated the role of MpARF1 in gemma development, which is traceable at the cellular level. In wild-type plants, a gemma initial first undergoes several transverse divisions to generate a single-celled stalk and a gemma proper, followed by rather synchronous longitudinal divisions in the latter. Mparf1 mutants often contained multicelled stalks and showed defects in the execution and timing of the longitudinal divisions. While wild-type gemmae finally generate two meristem notches, Mparf1 gemmae displayed various numbers of ectopic meristems. These results suggest that MpARF1 regulates formative cell divisions and axis formation through auxin responses. The mechanism for activator ARF regulation of pattern formation may be shared in land plants and therefore important for the general acquisition of three-dimensional body plans.

    DOI： 10.1093/pcp/pcx095

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  • Auxin-Mediated Transcriptional System with a Minimal Set of Components Is Critical for Morphogenesis through the Life Cycle in Marchantia polymorpha Reviewed

    Hirotaka Kato, Kimitsune Ishizaki, Masaru Kouno, Makoto Shirakawa, John L. Bowman, Ryuichi Nishihama, Takayuki Kohchi

    PLOS GENETICS   11 ( 5 )   e1005084   2015.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

    The plant hormone auxin regulates many aspects of plant growth and development. Recent progress in Arabidopsis provided a scheme that auxin receptors, TIR1/AFBs, target transcriptional co-repressors, AUX/IAAs, for degradation, allowing ARFs to regulate transcription of auxin responsive genes. The mechanism of auxin-mediated transcriptional regulation is considered to have evolved around the time plants adapted to land. However, little is known about the role of auxin-mediated transcription in basal land plant lineages. We focused on the liverwort Marchantia polymorpha, which belongs to the earliest diverging lineage of land plants. M. polymorpha has only a single TIR1/AFB (MpTIR1), a single AUX/IAA (MpIAA), and three ARFs (MpARF1, MpARF2, and MpARF3) in the genome. Expression of a dominant allele of MpIAA with mutations in its putative degron sequence conferred an auxin resistant phenotype and repressed auxin-dependent expression of the auxin response reporter proGH3:GUS. We next established a system for DEX-inducible auxin-response repression by expressing the putatively stabilized MpIAA protein fused with the glucocorticoid receptor domain (MpIAAmDII-GR). Repression of auxin responses in proMpIAA: MpIAAmDII-GR plants caused severe defects in various developmental processes, including gemmaling development, dorsiventrality, organogenesis, and tropic responses. Transient transactivation assays showed that the three MpARFs had different transcriptional activities, each corresponding to their phylogenetic classifications. Moreover, MpIAA and MpARF proteins interacted with each other with different affinities. This study provides evidence that pleiotropic auxin responses can be achieved by a minimal set of auxin signaling factors and suggests that the transcriptional regulation mediated by TIR1/AFB, AUX/IAA, and three types of ARFs might have been a key invention to establish body plans of land plants. We propose that M. polymorpha is a good model to investigate the principles and the evolution of auxin-mediated transcriptional regulation and its roles in land plant morphogenesis.

    DOI： 10.1371/journal.pgen.1005084

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  • Regulation of ROP GTPase cycling between active and inactive states is essential for vegetative organogenesis in <i>Marchantia polymorpha</i> Reviewed International journal

    Yuuki Sakai, Aki Ueno, Hiroki Yonetsuka, Tatsuaki Goh, Hirotaka Kato, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

    Development   151 ( 20 )   2024.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Company of Biologists  

    ABSTRACT

    Rho/Rac of plant (ROP) GTPases are plant-specific proteins that function as molecular switches, activated by guanine nucleotide exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs). The bryophyte Marchantia polymorpha contains single copies of ROP (MpROP), GEFs [ROPGEF and SPIKE (SPK)] and GAPs [ROPGAP and ROP ENHANCER (REN)]. MpROP regulates the development of various tissues and organs, such as rhizoids, gemmae and air chambers. The ROPGEF KARAPPO (MpKAR) is essential for gemma initiation, but the functions of other ROP regulatory factors are less understood. This study focused on two GAPs: MpROPGAP and MpREN. Mpren single mutants showed defects in thallus growth, rhizoid tip growth, gemma development, and air-chamber formation, whereas Mpropgap mutants showed no visible abnormalities. However, Mpropgap Mpren double mutants had more severe phenotypes than the Mpren single mutants, suggesting backup roles of MpROPGAP in processes involving MpREN. Overexpression of MpROPGAP and MpREN resulted in similar gametophyte defects, highlighting the importance of MpROP activation/inactivation cycling (or balancing). Thus, MpREN predominantly, and MpROPGAP as a backup, regulate gametophyte development, likely by controlling MpROP activation in M. polymorpha.

    DOI： 10.1242/dev.202928

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    Other Link： https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.202928/3564991/dev202928.pdf

  • Auxin signaling is essential for organogenesis but not for cell survival in the liverwort <i>Marchantia polymorpha</i> Reviewed International journal

    Hidemasa Suzuki, Hirotaka Kato, Megumi Iwano, Ryuichi Nishihama, Takayuki Kohchi

    The Plant Cell   35 ( 3 )   1058 - 1075   2022.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Auxin plays pleiotropic roles in plant development via gene regulation upon its perception by the receptors TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFBs). This auxin-regulated transcriptional control mechanism originated in the common ancestor of land plants. Although the complete loss of TIR1/AFBs causes embryonic lethality in Arabidopsis thaliana, it is unclear whether the requirement for TIR1-mediated auxin perception in cell viability can be generalized. The model liverwort Marchantia polymorpha has a minimal auxin signaling system with only a single TIR1/AFB, MpTIR1. Here we show by genetic, biochemical, and transcriptomic analyses that MpTIR1 functions as an evolutionarily conserved auxin receptor. Null mutants and conditionally knocked-out mutants of MpTIR1 were viable but incapable of forming any organs and grew as cell masses. Principal component analysis performed using transcriptomes at various developmental stages indicated that MpTIR1 is involved in the developmental transition from spores to organized thalli, during which apical notches containing stem cells are established. In Mptir1 cells, stem cell- and differentiation-related genes were up- and downregulated, respectively. Our findings suggest that, in M. polymorpha, auxin signaling is dispensable for cell division but is essential for three-dimensional patterning of the plant body by establishing pluripotent stem cells for organogenesis, a derived trait of land plants.

    DOI： 10.1093/plcell/koac367

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    Other Link： https://academic.oup.com/plcell/article-pdf/35/3/1058/49528410/koac367.pdf

  • R2R3-MYB transcription factor GEMMA CUP-ASSOCIATED MYB1 mediates the cytokinin signal to achieve proper organ development in Marchantia polymorpha Reviewed

    Shiori S. Aki, Tomoyo Morimoto, Taiki Ohnishi, Ayumi Oda, Hirotaka Kato, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Masaaki Umeda

    Scientific Reports   12 ( 1 )   2022.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Cytokinin, a plant hormone, plays essential roles in organ growth and development. The type-B response regulator-mediated cytokinin signaling is repressed by type-A response regulators and is conserved in the liverwort Marchantia polymorpha. Its signal coordinates the development of diverse organs on the thallus body, such as the gemma cup, rhizoid, and air pores. Here we report that the type-B response regulator MpRRB upregulates the expression of the R2R3-MYB transcription factor GEMMA CUP-ASSOCIATED MYB1 (MpGCAM1) in M. polymorpha. Whereas both Mpgcam1 and Mprrb knockout mutants exhibited defects in gemma cup formation, the Mpgcam1 Mprra double mutant, in which cytokinin signaling is activated due to the lack of type-A response regulator, also formed no gemma cups. This suggests that MpGCAM1 functions downstream of cytokinin signaling. Inducible overexpression of MpGCAM1 produced undifferentiated cell clumps on the thalli of both wild-type and Mprrb. However, smaller thalli were formed in Mprrb compared to the wild-type after the cessation of overexpression. These results suggest that cytokinin signaling promotes gemma cup formation and cellular reprogramming through MpGCAM1, while cytokinin signals also participate in activating cell division during thallus development.

    DOI： 10.1038/s41598-022-25684-3

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    Other Link： https://www.nature.com/articles/s41598-022-25684-3

  • The Renaissance and Enlightenment of Marchantia as a model system. International journal

    John L Bowman, Mario Arteaga-Vazquez, Frederic Berger, Liam N Briginshaw, Philip Carella, Adolfo Aguilar-Cruz, Kevin M Davies, Tom Dierschke, Liam Dolan, Ana E Dorantes-Acosta, Tom J Fisher, Eduardo Flores-Sandoval, Kazutaka Futagami, Kimitsune Ishizaki, Rubina Jibran, Takehiko Kanazawa, Hirotaka Kato, Takayuki Kohchi, Jonathan Levins, Shih-Shun Lin, Hirofumi Nakagami, Ryuichi Nishihama, Facundo Romani, Sebastian Schornack, Yasuhiro Tanizawa, Masayuki Tsuzuki, Takashi Ueda, Yuichiro Watanabe, Katsuyuki T Yamato, Sabine Zachgo

    The Plant cell   34 ( 10 )   3512 - 3542   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    The liverwort Marchantia polymorpha has been utilized as a model for biological studies since the 18th century. In the past few decades there has been a Renaissance in its utilization in genomic and genetic approaches to investigating physiological, developmental, and evolutionary aspects of land plant biology. The reasons for its adoption are similar to those of other genetic models, e.g. simple cultivation, ready access via its worldwide distribution, ease of crossing, facile genetics, and more recently, efficient transformation, genome editing, and genomic resources. The haploid gametophyte dominant life cycle of M. polymorpha is conducive to forward genetic approaches. The lack of ancient whole-genome duplications within liverworts facilitates reverse genetic approaches, and possibly related to this genomic stability, liverworts possess sex chromosomes that evolved in the ancestral liverwort. As a representative of one of the three bryophyte lineages, its phylogenetic position allows comparative approaches to provide insights into ancestral land plants. Given the karyotype and genome stability within liverworts, the resources developed for M. polymorpha have facilitated the development of related species as models for biological processes lacking in M. polymorpha.

    DOI： 10.1093/plcell/koac219

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  • Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts Reviewed

    Akida Jahan, Yuto Yamazaki, Mousona Islam, Totan Kumar Ghosh, Nami Yoshimura, Hirotaka Kato, Kimitsune Ishizaki, Akihisa Shinozawa, Yoichi Sakata, Daisuke Takezawa

    Frontiers in Plant Science   13   2022.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media {SA}  

    Phytohormone abscisic acid (ABA) plays a key role in stomata closure, osmostress acclimation, and vegetative and embryonic dormancy. Group B3 Raf protein kinases (B3-Rafs) serve as positive regulators of ABA and osmostress signaling in the moss Physcomitrium patens and the angiosperm Arabidopsis thaliana. While P. patens has a single B3-Raf called ARK, specific members of B3-Rafs among six paralogs regulate ABA and osmostress signaling in A. thaliana, indicating functional diversification of B3-Rafs in angiosperms. However, we found that the liverwort Marchantia polymorpha, belonging to another class of bryophytes, has three paralogs of B3-Rafs, MpARK1, MpARK2, and MpARK3, with structural variations in the regulatory domains of the polypeptides. By reporter assays of the P. patens ark line and analysis of genome-editing lines of M. polymorpha, we found that these B3-Rafs are functionally redundant in ABA response, with respect to inhibition of growth, tolerance to desiccation and expression of stress-associated transcripts, the majority of which are under the control of the PYR/PYL/RCAR-like receptor MpPYL1. Interestingly, gemmae in gemma cups were germinating only in mutant lines associated with MpARK1, indicating that dormancy in the gametophyte is controlled by a specific B3-Raf paralog. These results indicated not only conservation of the role of B3-Rafs in ABA and osmostress response in liverworts but also functional diversification of B3-Rafs, which is likely to have occurred in the early stages of land plant evolution.

    DOI： 10.3389/fpls.2022.952820

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  • On the Evolutionary Origins of Land Plant Auxin Biology Invited Reviewed

    John L. Bowman, Eduardo Flores Sandoval, Hirotaka Kato

    Cold Spring Harbor Perspectives in Biology   a040048 - a040048   2021.6

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cold Spring Harbor Laboratory  

    DOI： 10.1101/cshperspect.a040048

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  • Gemma cup and gemma development in Marchantia polymorpha Reviewed

    Hirotaka Kato, Yukiko Yasui, Kimitsune Ishizaki

    New Phytologist   228 ( 2 )   459 - 465   2020.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/nph.16655

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.16655

  • Evolution of vascular plants through redeployment of ancient developmental regulators

    Kuan-Ju Lu, Nicole van ’t Wout Hofland, Eliana Mor, Sumanth Mutte, Paul Abrahams, Hirotaka Kato, Klaas Vandepoele, Dolf Weijers, Bert De Rybel

    Proceedings of the National Academy of Sciences   117 ( 1 )   733 - 740   2019.12

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    Publishing type：Research paper (scientific journal)   Publisher：Proceedings of the National Academy of Sciences  

    Vascular plants provide most of the biomass, food, and feed on earth, yet the molecular innovations that led to the evolution of their conductive tissues are unknown. Here, we reveal the evolutionary trajectory for the heterodimeric TMO5/LHW transcription factor complex, which is rate-limiting for vascular cell proliferation in Arabidopsis thaliana . Both regulators have origins predating vascular tissue emergence, and even terrestrialization. We further show that TMO5 evolved its modern function, including dimerization with LHW, at the origin of land plants. A second innovation in LHW, coinciding with vascular plant emergence, conditioned obligate heterodimerization and generated the critical function in vascular development in Arabidopsis . In summary, our results suggest that the division potential of vascular cells may have been an important factor contributing to the evolution of vascular plants.

    DOI： 10.1073/pnas.1912470117

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    Other Link： https://pnas.org/doi/pdf/10.1073/pnas.1912470117

  • GEMMA CUP-ASSOCIATED MYB1, an Ortholog of Axillary Meristem Regulators, Is Essential in Vegetative Reproduction in Marchantia polymorpha Reviewed International coauthorship

    Hirotaka Kato

    Current Biology   29 ( 23 )   3987 - 3995.e5   2019.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.cub.2019.10.004

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  • Evolution of nuclear auxin signaling: Lessons from genetic studies with basal land plants Reviewed

    Hirotaka Kato, Ryuichi Nishihama, Dolf Weijers, Takayuki Kohchi

    Journal of Experimental Botany   69 ( 2 )   291 - 301   2018.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    Auxin plays critical roles in growth and development through the regulation of cell differentiation, cell expansion, and pattern formation. The auxin signal is mainly conveyed through a so-called nuclear auxin pathway involving the receptor TIR1/AFB, the transcriptional co-repressor AUX/IAA, and the transcription factor ARF with direct DNA-binding ability. Recent progress in sequence information and molecular genetics in basal plants has provided many insights into the evolutionary origin of the nuclear auxin pathway and its pleiotropic roles in land plant development. In this review, we summarize the latest knowledge of the nuclear auxin pathway gained from studies using basal plants, including charophycean green algae and two major model bryophytes, Marchantia polymorpha and Physcomitrella patens. In addition, we discuss the functional implication of the increase in genetic complexity of the nuclear auxin pathway during land plant evolution.

    DOI： 10.1093/jxb/erx267

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  • Auxin Produced by the Indole-3-Pyruvic Acid Pathway Regulates Development and Gemmae Dormancy in the Liverwort Marchantia polymorpha Reviewed

    D. Magnus Eklund, Kimitsune Ishizaki, Eduardo Flores-Sandoval, Saya Kikuchi, Yumiko Takebayashi, Shigeyuki Tsukamoto, Yuki Hirakawa, Maiko Nonomura, Hirotaka Kato, Masaru Kouno, Rishikesh P. Bhalerao, Ulf Lagercrantz, Hiroyuki Kasahara, Takayuki Kohchi, John L. Bowman

    PLANT CELL   27 ( 6 )   1650 - 1669   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC PLANT BIOLOGISTS  

    The plant hormone auxin (indole-3-acetic acid [IAA]) has previously been suggested to regulate diverse forms of dormancy in both seed plants and liverworts. Here, we use loss-and gain-of-function alleles for auxin synthesis-and signaling-related genes, as well as pharmacological approaches, to study how auxin regulates development and dormancy in the gametophyte generation of the liverwort Marchantia polymorpha. We found that M. polymorpha possess the smallest known toolkit for the indole-3-pyruvic acid (IPyA) pathway in any land plant and that this auxin synthesis pathway mainly is active in meristematic regions of the thallus. Previously a Trp-independent auxin synthesis pathway has been suggested to produce a majority of IAA in bryophytes. Our results indicate that the Trp-dependent IPyA pathway produces IAA that is essential for proper development of the gametophyte thallus of M. polymorpha. Furthermore, we show that dormancy of gemmae is positively regulated by auxin synthesized by the IPyA pathway in the apex of the thallus. Our results indicate that auxin synthesis, transport, and signaling, in addition to its role in growth and development, have a critical role in regulation of gemmae dormancy in M. polymorpha.

    DOI： 10.1105/tpc.15.00065

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  • Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha Reviewed

    Kimitsune Ishizaki, Maiko Nonomura, Hirotaka Kato, Katsuyuki T. Yamato, Takayuki Kohchi

    JOURNAL OF PLANT RESEARCH   125 ( 5 )   643 - 651   2012.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER JAPAN KK  

    The phytohormone auxin plays a pivotal role in various developmental aspects in land plants. However, little is known of the auxin response and distribution in non-vascular plants. In this study, we made transgenic plants of the liverwort Marchantia polymorpha which express the uidA (GUS) reporter gene under control of the soybean auxin-inducible promoter, ProGH3, and used it to indirectly monitor auxin-mediated transcriptional activation in planta. Transgenic plants carrying ProGH3:GUS showed GUS activity in an auxin-dependent manner. Histochemical GUS staining was observed at the bottom of gemma cups in the process of vegetative propagation. Significant GUS activity was also detected around the gametophyte-sporophyte junction as well as the developing sporophyte after fertilization. These results suggest that the activity of auxin is crucial in both gametophyte and sporophyte development in M. polymorpha, and that the mechanism for auxin-mediated transcriptional activation had already been established when plants emerged on the terrestrial environment.

    DOI： 10.1007/s10265-012-0477-7

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MISC

  • Different Impacts of Ni<sup>2+</sup> and Li<sup>+</sup> to Plants and Mycorrhiza Systems: Roles of Bioligands Forming Metal-Binding Complexes in Cells and Apoplasts Invited International coauthorship

    Masahiro Inouhe, Yui Katsuta, Aki Kato, Hirotaka Kato, Yoh Sakuma, Clemens Walther, Veenu Joon, Dharmendra K. Gupta

    Lithium and Nickel Contamination in Plants and the Environment   139 - 169   2024.2

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (other)   Publisher：WORLD SCIENTIFIC  

    DOI： 10.1142/9789811283123_0006

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  • The bHLH transcription factor MpHYPNOS regulates gemma dormancy in the liverwort Marchantia polymorpha

    Hirotaka Kato, Nami Yoshimura, Mikako Yoshikawa, Hideyuki Matsuura, Kosaku Takahashi, Daisuke Takezawa, Tomoyuki Furuya, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    bioRxiv   488978   2022.4

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    Publisher：Cold Spring Harbor Laboratory  

    DOI： 10.1101/2022.04.25.488978

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Presentations

  • Developmental roles of WIP, the evolutionarily conserved auxin-responsive transcription factor International coauthorship International conference

    Hirotaka Kato, Haruka Tsugawa, Dolf Weijers, Kimitsune Ishizaki

    International Marchantia Workshop 2024  2024.11 

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    Event date： 2024.11

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Hiroshima   Country：Japan  

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  • 苔類ゼニゴケにおける転写因子WIP 過剰発現株の解析 International coauthorship

    加藤 大貴, 津川 遥佳, Dolf Weijers, 石崎 公庸

    日本植物学会第88回大会  2024.9  日本植物学会

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    Event date： 2024.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：宇都宮   Country：Japan  

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  • 苔類ゼニゴケにおける気室および同化糸の形成異常株の解析

    佐野 あやな, 林 愛梨, 酒井 友希, 石崎 公庸, 佐久間 洋, 加藤 大貴

    日本植物学会第88回大会  2024.9  日本植物学会

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    Event date： 2024.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：宇都宮   Country：Japan  

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  • GROM, a direct target of GCAM1, regulates development of both vegetative and sexual reproductive organs in Marchantia polymorpha International conference

    Hirotaka Kato, Yukiko Yasui, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    International Plant Molecular Biology Conference 2024  2024.6 

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    Event date： 2024.6

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Cairns   Country：Australia  

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  • 苔類ゼニゴケにおける気室形成変異体の表現型解析および原因遺伝子同定

    佐野 あやな, 林 愛梨, 酒井 友希, 石崎 公庸, 佐久間 洋, 加藤 大貴

    第80回中国四国植物学会大会  2024.5  中国四国植物学会

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    Event date： 2024.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山   Country：Japan  

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  • スギナ栄養茎からの発芽・発根を制御する要因の解明

    内田 智尋, 佐久間 洋, 加藤 大貴

    第80回中国四国植物学会大会  2024.5  中国四国植物学会

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    Event date： 2024.5

    Language：Japanese   Presentation type：Poster presentation  

    Venue：岡山   Country：Japan  

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  • GROM, the target of GCAM1 regulates sexual reproductive organ development as well as gemma cup formation in Marchantia polymorpha

    Hirotaka Kato, Yukiko Yasui, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    The 65 yh Annual Meeting of The Japanese Society of Plant Physiologists  2024.3  The Japanese Society of Plant Physiologists

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Kobe   Country：Japan  

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  • 陸上植物においてオーキシンの下流で働く転写因子WIP の機能解析 International coauthorship

    加藤 大貴, Weijers Dolf, 石崎 公庸

    日本植物学会第87回大会  2023.9  日本植物学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌   Country：Japan  

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  • The single MYB gene GROM is required for gemma cup formation of the liverwort Marchantia polymorpha as a direct target of GCAM1

    Hirotaka Kato, Yukiko Yasui, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    The 64 yh Annual Meeting of The Japanese Society of Plant Physiologists  2023.3  The Japanese Society of Plant Physiologists

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    Event date： 2023.3

    Language：Japanese   Presentation type：Poster presentation  

    Venue：Sendai   Country：Japan  

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  • 苔類ゼニゴケの杯状体形成におけるsingle-MYB遺伝子GROMの機能解析

    加藤大貴, 安居佑季子, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

    第63回 日本植物生理学会 年会  2022.3  日本植物生理学会

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    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：つくば   Country：Japan  

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  • 陸上植物に保存されたオーキシン応答遺伝子WIP のゼニゴケにおける機能解析

    加藤大貴, 近藤侑貴, 深城英弘, Dolf Weijers, 石崎公庸

    日本植物学会第85回大会  2021.9  日本植物学会

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京   Country：Japan  

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  • Role of the core auxin-responsive gene family in a basal plant International conference

    Hirotaka Kato, Dolf Weijers, Kimitsune Ishizaki

    Secrets of stem cells underlying longevity and persistent growth in plants  2021.4 

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    Event date： 2021.4

    Language：English   Presentation type：Symposium, workshop panel (public)  

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  • Exploring the common mechanisms for stem cell propagation from vegetative reproduction of Marchantia polymorpha Invited

    Hirotaka Kato, Yukiko Yasui, Kimitsune Ishizaki

    2021.3 

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    Event date： 2021.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Research of auxin-WIP pathway in land plants International coauthorship

    Hirotaka Kato, Dolf Weijers, Kimitsune Ishizaki

    2020.11 

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    Event date： 2020.11

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 苔類ゼニゴケの杯状体形成に重要なsingle-MYBタンパク質の同定

    加藤大貴, 安居佑季子, 深城英弘, 三村徹郎, 石崎公庸

    日本植物学会第84回大会  2020.9 

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    Event date： 2020.9

    Presentation type：Oral presentation (general)  

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  • Identification of a novel regulator of gemma cup development in the liverwort Marchantia polymorpha

    Hirotaka Kato, Yukiko Yasui, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    2020.3  Japanese Society of Plant Physiologists

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    Event date： 2020.3

    Language：Japanese   Presentation type：Poster presentation  

    Venue：Osaka   Country：Japan  

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  • Design principles of a minimal auxin response system

    Hirotaka Kato, Sumanth K. Mutte, Hidemasa Suzuki, Yoshihiro Yoshitake, Emi Hainiwa, Isidro Crespo, Shubhajit Das, Tatyana Radoeva, Mattia Fontana, Mark Roosjen, Simon Lindhoud, Johannes Hohlbein, Jan Willem Borst, Roeland Boer, Ryuichi Nishihama, Takayuki Kohchi, Dolf Weijers

    Marchantia Workshop 2019  2019.9 

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    Event date： 2019.9

    Language：English  

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  • Design principle of auxin response system in Marchantia polymorpha Invited International coauthorship

    Hirotaka Kato, Sumanth Kumar Mutte, Tatyana Radoeva, Emi Hainiwa, Hidemasa Suzuki, Yoshihiro Yoshitake, Minami Katayama, Ryuichi Nishihama, Takayuki Kohchi, Dolf Weijers

    2019.9 

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    Event date： 2019.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

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  • A simple mechanistic model for auxin response in Marchantia polymorpha

    Hirotaka Kato

    Annual Theme Meeting of the Graduate School Experimental Plant Sciences Theme 1: Developmental Biology of Plants  2019.1 

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    Event date： 2019.1

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Leiden   Country：Netherlands  

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  • Differences of domain function between ancient auxin response factor subfamilies International conference

    Hirotaka Kato, Sumanth Kumar Mutte, Dolf Weijers

    EMBO Workshop: New shores in land plant evolution  2018.6 

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    Event date： 2018.6

    Presentation type：Poster presentation  

    Venue：Lisbon   Country：Portugal  

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  • Functional Differences Between Ancient Subfamilies of Auxin Response Factors International conference

    Hirotaka Kato, Dolf Weijers

    The 65th NIBB Conference - Marchantia Workshop 2017  2017.12 

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    Event date： 2017.12

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

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  • Dissecting functional diversification among auxin response factors in land plants International conference

    Hirotaka Kato

    2017.9 

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    Event date： 2017.9

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ghent   Country：Belgium  

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  • Dissecting functional diversification among auxin response factors in land plants

    Hirotaka Kato, Sumanth Kumar Mutte, Dolf Weijers

    2017.3 

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    Event date： 2017.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

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  • Dissecting functional diversification among the three types of ARFs in land plants International conference

    Hirotaka Kato, Dolf Weijers

    5th international conference on auxin research – Auxin2016  2016.10 

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    Event date： 2016.10

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Sanya   Country：China  

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  • Regulation of cell division pattern by the sole activator-type auxin response factor in Marchantia polymorpha International conference

    Hirotaka Kato, Kimitsune Ishizaki, Ryuichi Nishihama, Dolf Weijers, Takayuki Kohch

    EMBO Workshop: New model systems for early land plant evolution  2016.6 

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    Event date： 2016.6

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Vienna   Country：Austria  

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  • A simple auxin responsesystem in the liverwort Marchantia polymorpha

    Hirotaka Kato

    Annual Theme Meeting of the Graduate School Experimental Plant Sciences Theme 1: Developmental Biology of Plants  2016.1 

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    Event date： 2016.1

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Wageningen   Country：Netherlands  

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  • Transcriptional activities and protein interactions of AUXIN RESPONSE FACTORs from Marchantia polymorpha

    加藤大貴, 鈴木秀政, 山岡尚平, 西浜竜一, 石崎公庸, 河内孝之

    48th Annual Meeting of the Japanese Society of Developmental Biologists  2015.6 

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    Language：English  

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  • 苔類ゼニゴケにおけるオーキシン受容メカニズムの解析

    加藤大貴, 鈴木秀政, 山岡尚平, 西浜竜一, 石崎公庸, 河内孝之

    第56回日本植物生理学会年会  2015.3 

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    Event date： 2015.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京   Country：Japan  

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  • Functional divergence of AUXIN RESPONSE FACTORs in Marchantia polymorpha

    Kato Hirotaka, Kouno Masaru, Shirakawa Makoto, Ishizaki Kimitsune, Nishihama Ryuichi, Kohchi Takayuki

    The Janapese Society for Chemical Regulation of Plants  2014.10  The Janapese Society for Chemical Regulation of Plants

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    Event date： 2014.10

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 転写因子の相互作用によるゼニゴケのオーキシン信号伝達ネットワーク

    加藤大貴, 神埜勝, 白川一, 石崎公庸, 西浜竜一, 河内孝之

    第55回日本植物生理学会年会  2014.3 

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    Language：Japanese  

    Venue：富山   Country：Japan  

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  • 苔類ゼニゴケにおけるAUX/IAA‐ARF相互作用を介したオーキシン転写制御機構の解析

    加藤大貴, 神埜勝, 白川一, 石崎公庸, 西浜竜一, 河内孝之

    第77回日本植物学会大会  2013.9 

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    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌   Country：Japan  

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  • ゼニゴケ形態形成におけるMpIAA を介したオーキシン転写制御の多面的役割

    加藤大貴, 石崎公庸, 河内孝之

    日本植物学会第76回大会  2012.9 

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    Event date： 2012.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：姫路   Country：Japan  

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  • 複雑なオーキシン応答をゼニゴケを用いて単純に理解する Invited

    加藤大貴, 石崎公庸, 河内孝之

    第53回日本植物生理学会年会  2012.3 

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    Event date： 2012.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都   Country：Japan  

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  • 苔類ゼニゴケにおけるオーキシン転写制御機構の役割

    加藤大貴, 石崎公庸, 河内孝之

    第84回日本生化学会大会  2011.9 

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    Event date： 2011.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都   Country：Japan  

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  • 苔類ゼニゴケにおけるMpIAAを介したオーキシン信号伝達の機能解析

    加藤大貴, 石崎公庸, 大和勝幸, 河内孝之

    第52回日本植物生理学会年会  2011.3 

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    Event date： 2011.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：仙台   Country：Japan  

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  • オーキシン信号伝達の基本的な因子は苔類ゼニゴケに保 存されている

    加藤大貴, 石崎公庸, 大和勝幸, 河内孝之

    第51回日本植物生理学会年会  2010.3 

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    Event date： 2010.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：熊本   Country：Japan  

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  • 四国における Google Map を用いたダンチクの分布調査 ―環境要因による生息地の予測 ―

    佐久間 洋, 永瀬 真, 吉川 朝彦, 堀澤 栄, 加藤 大貴, 井上 雅裕

    日本植物学会第88回大会  2024.9  日本植物学会

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    Event date： 2024.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：宇都宮   Country：Japan  

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  • 懸濁培養細胞とウキクサ葉状体のリチウム・ニッケルイオン集積性とリガンド生成能

    井上 雅裕, 中西 亮介, 加藤 晶, 加藤 大貴, 佐久間 洋

    日本植物学会第88回大会  2024.9  日本植物学会

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    Event date： 2024.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：宇都宮   Country：Japan  

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  • A mutation of CHLH confers resistance to PPO inhibitors in Marchantia polymorpha

    Rito Kitaoka, Satoshi Komaki, Yuuki Sakai, Hirotaka Kato, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizak

    The 65 yh Annual Meeting of The Japanese Society of Plant Physiologists  2024.3  The Japanese Society of Plant Physiologists

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Kobe   Country：Japan  

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  • The bHLH transcriptional factor MpHYPNOS regulates gemma dormancy in Marchantia polymorpha

    Nami Yoshimura, Mikako Yoshikawa, Arisa Yasuda, Hirotaka Kato, Yuuki Sakai, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

    The 65 yh Annual Meeting of The Japanese Society of Plant Physiologists  2024.3  The Japanese Society of Plant Physiologists

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Kobe   Country：Japan  

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  • ゼニゴケCHLH遺伝子の変異はPPO阻害除草剤耐性を付与する

    北岡 理杜, 小牧 知史, 酒井 友希, 加藤 大貴, 近藤 侑貴, 深城 英弘, 石崎 公庸

    日本植物学会第87回大会  2023.9  日本植物学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌   Country：Japan  

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  • ゼニゴケの器官形成を制御するROP シグナリング

    酒井 友希, 米塚 広樹, 上野 亜紀, 加藤 大貴, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

    日本植物学会第87回大会  2023.9  日本植物学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌   Country：Japan  

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  • MpHYPNOS はABA 依存的および⾮依存的に無性芽の休眠を制御する

    芳村 那美, 吉川 実樺子, 安田 有沙, 加藤 大貴, 酒井 友希, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

    日本植物学会第87回大会  2023.9  日本植物学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌   Country：Japan  

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  • アオウキクサフロンドによる金属イオンNa+, Li+, Cs+, Ni2+の吸収及び耐性評価

    井上 雅裕, 植田 慶和, 川口 瑞貴, 加藤 大貴, 佐久間洋, Dharmendra K Gupta

    日本植物学会第87回大会  2023.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：札幌   Country：Japan  

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  • Role of ROP signaling in the growth and organogenesis of Marchantia polymorpha

    Yuuki Sakai, Hiroki Yonetsuka, Aki Ueno, Hirotaka Kato, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

    The 64 yh Annual Meeting of The Japanese Society of Plant Physiologists  2023.3  The Japanese Society of Plant Physiologists

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    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Sendai   Country：Japan  

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  • Differential effects of excessive mM-order-levels of alkaline-metal ions (Na+, Li+, Cs+) on growth patterns of 6 plant seedlings cultivated under the same soil and medium conditions

    Masahiro Inoue, Yui Katsuta, Hirotaka Kato, Yoh Sakuma, Dharmendra K. Gupta

    The 64 yh Annual Meeting of The Japanese Society of Plant Physiologists  2023.3  The Japanese Society of Plant Physiologists

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    Event date： 2023.3

    Language：Japanese   Presentation type：Poster presentation  

    Venue：Sendai   Country：Japan  

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  • Mechanisms of MpHYPNOS-mediated gemma dormancy in Marchantia polymorpha

    Nami Yoshimura, Mikako Yoshikawa, Arisa Yasuda, Hirotaka Kato, Yuuki Sakai, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

    The 64 yh Annual Meeting of The Japanese Society of Plant Physiologists  2023.3  The Japanese Society of Plant Physiologists

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    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Sendai   Country：Japan  

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  • The role of MYBCC-SPX module in phosphate response of nonvascular plant Marchantia polymorpha

    Hinatamaru Fukumura, Ginga Kitaura, Hirotaka Kato, Yuuki Sakai, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

    The 64 yh Annual Meeting of The Japanese Society of Plant Physiologists  2023.3  The Japanese Society of Plant Physiologists

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    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Sendai   Country：Japan  

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  • コケ植物ゼニゴケの脅威の繁殖力を支える分子基盤 Invited

    酒井 友希, 加藤 大貴, 安居 佑季子, 石崎 公庸

    日本植物学会第86回大会  2022.9  日本植物学会

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：京都   Country：Japan  

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  • コケ植物ゼニゴケのリン欠乏応答を制御するMYBCC 型転写因子の機能解析

    福村 日向丸, 北浦 銀河, 加藤 大貴, 酒井 友希, 近藤, 侑貴, 深城 英弘, 三村 徹郎, 石崎 公庸

    日本植物学会第86回大会  2022.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：京都   Country：Japan  

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  • PPO阻害除草剤耐性ゼニゴケの単離と解析

    北岡 理杜, 小牧 知史, 酒井 友希, 加藤 大貴, 近藤 侑貴, 深城 英弘, 石崎 公庸

    日本植物学会第86回大会  2022.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：京都   Country：Japan  

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  • ゼニゴケ無性芽の休眠を促進するMpHYPNOSの機能

    芳村 那美, 𠮷川実樺子, 安田 有沙, 加藤 大貴, 酒井 友希, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

    日本植物学会第86回大会  2022.9  日本植物学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都   Country：Japan  

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  • ゼニゴケ無性芽におけるMpHYPNOS を介した休眠制御

    芳村那美, 𠮷川実樺子, 安田有沙, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

    第63回 日本植物生理学会 年会  2022.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 非維管束植物ゼニゴケのリン欠乏応答におけるMYBCC-SPX モジュールの機能

    福村日向丸, 北浦銀河, 加藤大貴, 酒井友希, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

    第63回 日本植物生理学会 年会  2022.3 

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  • 基部陸上植物ゼニゴケにおけるリンの吸収・分配・貯蔵のメカニズム

    佐藤しおり, 福村日向丸, 加藤大貴, 酒井友希, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

    日本植物学会第86回大会  2021.9  日本植物学会

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京   Country：Japan  

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  • 基部陸上植物ゼニゴケのリン欠乏応答におけるMpMYBCC-MpSPX モジュールの機能

    福村日向丸, 北浦銀河, 加藤大貴, 酒井友希, 深城英弘, 近藤侑貴, 三村徹郎, 石崎公庸

    日本植物学会第87回大会  2021.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京   Country：Japan  

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  • ゼニゴケの葉状体再生におけるGCAM1 およびGC1L の機能

    田中涼葉, 加藤大貴, 安居佑季子, 近藤侑貴, 深城英弘, 石崎公庸

    日本植物学会第89回大会  2021.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京   Country：Japan  

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  • ゼニゴケ無性芽におけるMpHYPNOS を介した休眠制御

    芳村那美, 吉川実樺子, 安田有沙, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

    日本植物学会第88回大会  2021.9  日本植物学会

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京   Country：Japan  

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  • ゼニゴケ葉状体の形態形成におけるMpREN の機能解析

    米塚広樹, 上野亜紀, 樋渡琢真, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

    日本植物学会第90回大会  2021.9  日本植物学会

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    Event date： 2021.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京   Country：Japan  

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  • Functional characterization of LAX PANICLE2 homologous in the liverwort Marchantia polymorpha

    Naho Mehara, Kato Hirotaka, Yuuki Sakai, Yuki Kondo, Mimura Tetsuro, Hidehiro Fukaki, Kimitsune Ishizaki

    2021.3 

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    Event date： 2021.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 基部陸上植物ゼニゴケにおけるリン酸欠乏に応答した遺伝子転写制御

    北浦銀河, 佐藤しおり, 加藤大貴, 深城英弘, 三村徹郎, 石崎公庸

    日本植物学会第84回大会  2020.9 

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    Event date： 2020.9

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • ゼニゴケの再生を制御する2つのR2R3-MYB 型転写因子の機能解析

    安居佑季子, 加藤大貴, 石田咲子, 西浜竜一, 深城英弘, 三村徹郎, 河内孝之, 石崎公庸

    第61回日本植物生理学会年会  2020.3 

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    Event date： 2020.3

    Presentation type：Poster presentation  

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  • Two R2R3-MYB Transcription Factors Regulate Acquisition of Pluripotency in Vegetative Cells of Marchantia polymorpha

    Yukiko Yasui, Shigeyuki Tsukamoto, Sakiko Ishida, Ryuichi Nishihama, Hirotaka Kato, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Kimitsune Ishizaki

    Marchantia Workshop 2019  2019.9 

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    Event date： 2019.9

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  • GEMMA CUP-ASSOCIATED MYB1, an orthologue of axillary meristem regulator, is essential for vegetative reproduction in the liverwort Marchantia polymorpha

    Kimitsune Ishizaki, Yukiko Yasui, Shigeyuki Tsukamoto, Hirotaka Kato, Klaus Theres, Takayuki Kohchi

    Marchantia Workshop 2019  2019.9 

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    Event date： 2019.9

    Language：English  

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  • ゼニゴケ唯一のTIR1/AFBホモ ログMpTIR1は生存に必須でない が正常な器官形成に必須である

    鈴木秀政, 加藤大貴, 西浜竜一, 河内 孝之

    日本植物学会第83回大会  2019.9 

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    Event date： 2019.9

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  • ゼニゴケで唯一のオーキシン受容体遺伝子MpTIR1は発生に必要であるが細胞の生存には必須ではない

    鈴木秀政, 加藤大貴, 加藤大貴, 山岡尚平, 西浜竜一, 河内孝之

    日本農芸化学会大会講演要旨集(Web)  2018.3 

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    Event date： 2018.3

    Language：Japanese  

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  • The Sole Auxin Receptor Gene MpTIR1 Is Disprensable for Cell Survival but Required for Development in the Liverwort Marchantia polymorpha International conference

    Hidemasa Suzuki, Hirotaka Kato, Shohei Yamaoka, Ryuichi Nishihama, Takayuki Kohchi

    The 65th NIBB Conference - Marchantia Workshop 2017  2017.12 

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    Event date： 2017.12

    Language：English   Presentation type：Poster presentation  

    Venue：Okazaki   Country：Japan  

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  • 核オーキシンシグナリングによるゼニゴケ幹細胞機能調節

    鈴木秀政, 加藤大貴, 片山みなみ, 胚庭瑛実, 山岡尚平, 西浜竜一, 河内孝之

    日本植物学会第81回大会  2017.9 

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    Event date： 2017.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：野田   Country：Japan  

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  • 苔類ゼニゴケにおけるMpTIR1を介したオーキシン受容機構と生理機能の解析

    鈴木秀政, 加藤大貴, 山岡尚平, 西浜竜一, 河内孝之

    日本農芸化学会大会講演要旨集(Web)  2017.3 

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    Event date： 2017.3

    Language：Japanese  

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  • Origin and evolution of nuclear auxin pathway components International conference

    Sumanth Kumar Mutte, Hirotaka Kato, Dolf Weijers

    5th international conference on auxin research – Auxin2016  2016.10 

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    Event date： 2016.10

    Language：English   Presentation type：Poster presentation  

    Venue：Sanya   Country：China  

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  • Roles of transcriptional auxin signaling in the liverwort Marchantia polymorpha International conference

    Takayuki Kohchi, Hirotaka Kato, Hidemasa Suzuki, Minami Katayama, Masaru Kouno, Mayuko Takeda, Sakiko Ishida, Kimitsune Ishizaki, Ryuichi Nishihama

    5th international conference on auxin research – Auxin2016  2016.10 

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    Event date： 2016.10

    Presentation type：Oral presentation (invited, special)  

    Venue：Sanya   Country：China  

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  • The single-copy auxin receptor gene MpTIR1 regulates various development and differentiation in the liverwort Marchantia polymorpha

    Hidemasa Suzuki, Hirotaka Kato, Shohei Yamaoka, Ryuichi Nishihama, Takayuki Kohchi

    Auxin 2016  2016.10 

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    Event date： 2016.10

    Language：English  

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  • The single-copy auxin receptor gene MpTIR1 plays pivotal roles in development of the liverwort Marchantia polymorpha International conference

    Hidemasa Suzuki, Hirotaka Kato, Shohei Yamaoka, Ryuichi Nishihama, Takayuki Kohchi

    EMBO Workshop: New model systems for early land plant evolution  2016.6 

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    Event date： 2016.6

    Language：English   Presentation type：Poster presentation  

    Venue：Vienna   Country：Austria  

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  • 苔類ゼニゴケおける単一のオーキシン受容体遺伝子MpTIR1による 多面的な生長調節の解析

    鈴木秀政, 加藤大貴, 山岡尚平, 西浜竜一, 河内孝之

    第57回日本植物生理学会年会  2016.3 

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    Event date： 2016.3

    Language：Japanese  

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  • ゼニゴケのオーキシン応答転写因子ARF1は無性芽の細胞分裂パターンを制御する

    神埜勝, 加藤大貴, 武田真由子, 石崎公庸, 西浜竜一, 河内孝之

    第56回日本植物生理学会年会  2015.3 

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    Event date： 2015.3

    Language：Japanese  

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  • 苔類ゼニゴケにおけるオーキシン受容体MpTIR1の生理機能の解析

    鈴木秀政, 加藤大貴, 山岡尚平, 西浜竜一, 河内孝之

    第56回日本植物生理学会年会  2015.3 

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    Event date： 2015.3

    Language：Japanese  

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  • Transcriptional activities and protein interactions of auxin signaling factors from Marchantia polymorpha International conference

    Hirotaka Kato, Masaru Kouno, Makoto Shirakawa, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi

    Marchantia Workshop 2014  2014.12 

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    Event date： 2014.12

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Kobe   Country：Japan  

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  • Regulation of gemma development by an AUXIN RESPONSE FACTOR1 in Marchantia polymorpha

    Kouno Masaru, Kato Hirotaka, Takeda Mayuko, Ishizaki Kimitsune, Nishihama Ryuichi, Kohchi Takayuki

    The Janapese Society for Chemical Regulation of Plants  2014.10  The Janapese Society for Chemical Regulation of Plants

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    Event date： 2014.10

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • ゼニゴケのオーキシン応答転写因子ARF1は無性芽の発生を制御する

    神埜勝, 加藤大貴, 武田真由子, 石崎公庸

    日本植物学会第78回大会  2014.9 

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    Event date： 2014.9

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  • ゼニゴケ研究地平への投射:オーキシン信号伝達を例に

    河内孝之, 加藤大貴, 西浜竜一, 大和勝幸, 石崎公庸

    第55回日本植物生理学会年会  2014.3 

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    Event date： 2014.3

    Language：Japanese  

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  • ゼニゴケのオーキシン信号伝達因子MpARF1による無性芽発生制御

    神埜勝, 加藤大貴, 武田真由子, 石崎公庸, 西浜竜一, 河内孝之

    第77回日本植物学会大会  2013.9 

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    Event date： 2013.9

    Language：Japanese  

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  • Auxin response and signaling factors in Marchantia polymorpha Invited International conference

    Hirotaka Kato, Kimitsune Ishizaki, Maiko Nonomura, Katsuyuki T. Yamato, Takayuki Kohchi

    XVIII International Botanical Congress 

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    Event date： 2011.7

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue：Melbourne   Country：Australia  

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Awards

  • UFW Research Award 2019

    2019.9   University Fund Wageningen  

    Sumanth MUTTE, Hirotaka KATO

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  • Journal of Plant Research Best Paper Award

    2013.6   The Botanical Society of Japan  

    Kimitsune Ishizaki, Maiko Nonomura, Hirotaka Kato, Katsuyuki T. Yamato, Takayuki Kohchi

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Research Projects

  • 陸上植物に共通するオーキシン-WIP経路の多面的な発生制御の原理を探る

    2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    加藤 大貴

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

    植物ホルモンのオーキシンとその下流で機能するWIPは陸上植物の発生において多面的な役割を果たすが、多様な機能を果たすための分子メカニズムは未だ明らかになっていない。本研究ではゼニゴケをモデルに（１）WIPの標的遺伝子の網羅的探索と結合配列の推定、（２）生化学的・遺伝学的アプローチによるWIPと相互作用するタンパク質の探索、（３）組織特異的な複合体形成による下流応答制御の検証を計画しており、本年度は（１）、（２）について以下のような成果を得た。
    （１）WIPが直接制御する遺伝子を網羅的に探索するためクロマチン免疫沈降シーケンス解析（ChIP-seq）を計画している。実験に必要な植物材料としてエストロゲン依存的にタグ（GFP）を融合したWIPタンパク質を発現する株の作成を試みたが、表現型を示す株が得られなかった。融合したタグがWIPの機能を阻害している可能性を考え、現在GFPよりも分子量が小さく機能阻害を起こしにくいと予想されるFLAGタグを付加したものを作成中である。また他の転写因子を用いた実験ではあるが、GFP融合タンパク質発現ゼニゴケを用いたChIP実験系の立ち上げに成功した。
    （２）WIPの相互作用因子を探索するため、エストロゲン依存的にタグなしのWIPを発現する株（誘導により枯死する）を背景とした抑圧変異体スクリーニングを計画していた。変異処理を行う胞子を取得するため、野生型との掛け合わせを行ってF1胞子を取得した。F1胞子の中から親株と同様にエストロゲン処理により枯死する雌雄株を選抜し、掛け合わせを行なった。しかしF2胞子の形成率が悪く、また得られたF2胞子の発芽率も非常に低く、純遺伝学的スクリーニングを行うのには不十分であった。導入コンストラクトが何らかの理由で胞子形成を阻害していると考えられる。

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  • Molecular mechanism for auxin-mediated regulation of cell division plane in land plants

    2019.8 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity Start-up

    Kato Hirotaka

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    Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）

    The plant hormone auxin plays critical and versatile roles in the growth and development of all land plants. My recent study identified WIP family as a common auxin-responsive gene in land plants. To investigate the role of auxin-WIP pathway in land plants, this study focused on the liverwort Marchantia polymorpha of which genome encodes only a single WIP gene (MpWIP).
    Expression analysis revealed that MpWIP was highly expressed in the ventral side of apical meristem and gemma cup. Loss-of-function mutants generated by CRISPR/Cas9 method showed reduction of rhizoid elongation and dense formation of gemma cups. RNA-seq analyses using the mutants and overexpression lines identified downstream candidates of MpWIP. Comparison with published transcriptome data from Arabidopsis thaliana provided insights into the conserved role of WIP family in land plants.

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  • 多様なオーキシン応答を引き起こす転写調節機構の基本原理

    2012 - 2013

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    加藤 大貴

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    Grant amount：\1800000 （ Direct Cost: \1800000 ）

    本研究は苔類ゼニゴケをモデルにTIRI/AFB-AUX/IAA-ARF相互作用を介したオーキシン転写制御機構の有無とその役割について解析してきた。
    ゼニゴケ形態形成におけるオーキシン信号伝達の役割を調べるため、ステロイドホルモン依存的にオーキシン応答を抑制できる形質転換体を作成し、様々な成長段階での表現型を解析した。その結果、MplAAを介したオーキシン応答が細胞の分化・伸長に関わり、配偶体における腹側化の促進や器官発生、胞子体の発生など生活環全体にわたって多様な現象を制御していることが示唆された。またMpIAAとMpARFs間の相互作用をBiFC法とY2H法により解析した。その結果、MpIAAとMpARFsはMpARF3同士以外のすべての組み合わせで相互作用できることが明らかになり、またそれぞれの相互作用の強度には違いがあることも明らかになった。次にMpIAAがオーキシン依存的に分解されるかどうかを調べるため、MplAAの予測分解調節領域と蛍光タンパク質の融合タンパク質発現株を作成したところ、著しくタンパク質が不安定化されていることが明らかになった。さらに、ゼニゴケにおけるオーキシン応答遺伝子をRNA-seq解析により網羅的に探索した。その結果、既知のオーキシン応答遺伝子であるMpGH3を含む152の発現変動遺伝子候補を同定した。
    本研究の成果から、苔類ゼニゴケにおいてもAUX/IMの分解、AUX/IM-ARF相互作用を介した転写制御機構が保存されていることが明らかになった。またゼニゴケは他の植物種に比べて非常に単純なオーキシン信号伝達機構を用いて、様々な生理応答を制御していることも明らかになった。今後、それぞれのMpARFによる下流遺伝子の発現制御機構が研究されることで、単純な転写調節機構が複雑なオーキシン応答を生み出すメカニズムの理解が進むと期待される。

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