Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

researchmap

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.rineng.2024.103183

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

DOI： 10.1371/journal.pntd.0012599

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10653-024-02249-z

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

DOI： 10.3389/fcimb.2024.1456700

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/arch.22137

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.bbrc.2024.149971

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s13071-024-06300-x

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

DOI： 10.3389/fcimb.2024.1360438

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

DOI： 10.1371/journal.pone.0298656

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

DOI： 10.3390/tropicalmed9030060

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.virol.2024.109982

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：European Centre for Disease Control and Prevention (ECDC)  

DOI： 10.2807/1560-7917.es.2024.29.8.2300277

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Royal Society  

DOI： 10.1098/rsos.231373

researchmap

Other Link： https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.231373

Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acs.estlett.3c00820

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.29313/gmhc.v11i3.12749

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fenvs.2023.1205561

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fcimb.2023.1252656

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fmicb.2023.1230548

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1155/2023/6255586

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

DOI： 10.1371/journal.pone.0289056

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.scitotenv.2023.162258

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

The cranefly (Tipuloidea) fauna of the Western Balkans is still poorly known. In this study, occurrence data of 77 species is reported, of which two species are newly recorded for Albania, eight species for Bosnia and Herzegovina, twelve for Croatia, and seven for Slovenia, respectively. A new species, Baeoura neretvaensis Kolcsár & d’Oliveira, sp. nov. is described from Bosnia and Herzegovina, Montenegro, and Slovenia. Images of the habitus, wing, and male and female terminalia of the new species are provided. Furthermore, images of male terminalia and wings of thirteen additional species are presented.

DOI： 10.3897/zookeys.1157.98997

researchmap

Other Link： https://zookeys.pensoft.net/article/98997/download/xml/

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Regional Euro-Asian Biological Invasions Centre Oy (REABIC)  

DOI： 10.3391/bir.2023.12.1.11

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/d15030336

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/jbi.14537

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jbi.14537

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s00442-023-05321-0

researchmap

Other Link： https://link.springer.com/article/10.1007/s00442-023-05321-0/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.dib.2022.108599

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

Japanese species of the subgenera Oreophila Lackschewitz and Parormosia Alexander of the genus Ormosia Rondani (Limoniidae) are revised. Two new species Ormosia (Oreophila) komazawai Kato & Kolcsár, sp. nov. and Ormosia (Parormosia) phalara Kato & Kolcsár, sp. nov. are described. The identities of all Japanese species of the two subgenera are clarified and redescribed with images of habitus and wings, and drawings of male and female terminalia. The first DNA barcode sequences of the species Ormosia (Parormosia) diversipes Alexander and Ormosia (Parormosia) phalara Kato & Kolcsár, sp. nov. are also provided. A key to, and distribution maps of, the Japanese species are provided.

DOI： 10.3897/zookeys.1132.86022

researchmap

Other Link： https://zookeys.pensoft.net/article/86022/download/xml/

Authorship：Last author   Language：Japanese   Publishing type：Research paper (conference, symposium, etc.)  

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

(1) Background: This paper will present an elaboration of the risk assessment methodology by Deutsche Gesellschaft für Internationale Zusammenarbeit GmbH (GIZ), Eurac Research and United Nations University Institute for Environment and Human Security (UNU-EHS) for the assessment of dengue. (2) Methods: We validate the risk assessment model by best-fitting it with the number of dengue cases per province using the least-square fitting method. Seven out of thirty-four provinces in Indonesia were chosen (North Sumatra, Jakarta Capital, West Java, Central Java, East Java, Bali and East Kalimantan). (3) Results: A risk assessment based on the number of dengue cases showed an increased risk in 2010, 2015 and 2016 in which the effects of El Nino and La Nina extreme climates occurred. North Sumatra, Bali, and West Java were more influenced by the vulnerability component, in line with their risk analysis that tends to be lower than the other provinces in 2010, 2015 and 2016 when El Nino and La Nina occurred. (4) Conclusion: Based on data from the last ten years, in Jakarta Capital, Central Java, East Java and East Kalimantan, dengue risks were mainly influenced by the climatic hazard component while North Sumatra, Bali and West Java were more influenced by the vulnerability component.

DOI： 10.3390/tropicalmed7080172

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0270411

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Using a stable isotope two- and three-source mixing model, we evaluated trophic contributions of reservoir-derived plankton, epilithon, and terrestrial leaves to stream macroinvertebrates at two dam upstream and four dam outlet reaches. We compared four different habitat types co-occurring within the reaches: lotic habitat, bar-head lentic habitat, bar-tail lentic habitat, and isolated pond on sandy bars. Filter-feeders, which provide the highest trophic contributions of reservoir-derived plankton, did not significantly differ among habitats, whereas collector-gatherers and predators differed significantly. Thus, the three-source model analysis for collector-gatherers and predators indicated a lower proportion of reservoir-derived plankton in lentic habitat than in lotic habitat. This difference in food source composition was reflected in the lower trophic contribution of plankton to lentic species. Macroinvertebrates in lentic habitats fed on sources less mixed with reservoir-derived plankton; therefore, the trophic impact of reservoirs was indistinctive at the community level, indicating that lentic habitats can function as trophic refugia to mitigate the trophic impact of reservoirs. Our findings demonstrate that habitat heterogeneity potentially provides a foundation for determining the trophic contribution of food webs while affecting communities when there is anthropogenic input of food resources to the habitat.

DOI： 10.1007/s10750-022-04866-0

Scopus

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

Abstract

Dengue Hemorrhagic Fever (DHF) is a major mosquito-borne viral disease. Studies have reported a strong correlation between weather, the abundance of Aedes aegypti, the vector of DHF virus, and dengue incidence. However, this conclusion has been based on the general climate pattern of wide regions. In general, however, the human population, level of infrastructure, and land-use change in rural and urban areas often produce localized climate patterns that may influence the interaction between climate, vector abundance, and dengue incidence. Thoroughly understanding this correlation will allow the development of a customized and precise local early warning system. To achieve this purpose, we conducted a cohort study, during January-December 2017, in 16 districts in Bandung, West Java, Indonesia. In the selected areas, local weather stations and modified light mosquito traps were set up to obtain data regarding daily weather and the abundance of adult female Ae. aegypti. A generalized linear model was applied to analyze the effect of local weather and female adult Ae. aegypti on the number of dengue cases. The result showed a significant non-linear correlation among mosquito abundance, maximum temperature, and dengue cases. Using our model, the data showed that the addition of a single adult Ae. aegypti mosquito increased the risk of dengue infection by 1.8%, while increasing the maximum temperature by one degree decreased the risk by 17%. This finding suggests specific actionable insights needed to supplement existing mosquito eradication programs.

DOI： 10.1093/jme/tjac005

Web of Science

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

It is essential to explore and define the level of taxonomic identification and quantification approaches for diversity and biomonitoring studies, given their potential influence on assessing and interpreting ecological outcomes. This study assessed the response of benthic macroinvertebrate communities to restore and construct gravel bars in a dam-impacted river, with non-dam influenced tributaries serving as reference sites. Additionally, we evaluated the performance of different taxonomic and numerical (i.e., abundance vs. presence/absence data) resolutions of DNA metabarcoding with consequent comparison to morphology-based identification and how it affects assessment outcomes. DNA metabarcoding detected 93% of the morphologically identified individuals and provided finer taxonomic resolution. We also detected significant correlations between morphological sample abundance, biomass, and DNA metabarcoding-read abundance. Furthermore, we observed a relatively high and significant congruence in macroinvertebrate community structure and composition between different taxonomic and numerical resolutions of both methods. This indicates a satisfactory surrogacy between the two approaches and their varying identification levels and data transformation. Additionally, community–environmental associations were significant for all datasets but showed significant varying associations with physicochemical parameters. Our observations imply that coarser taxonomic resolution could be highly advantageous for DNA metabarcoding-based applications in situations where the lack of taxonomic information, e.g., poor reference database, might severely affect biological assessment quality.

DOI： 10.1016/j.ecolind.2021.108508

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

Helopeltis bakeri Poppius is a major insect pest of cacao, Theobroma cacao L. in the Philippines. It is one of the four Helopeltis species endemic to the Philippines, easily recognizable for its sexual dimorphism. Up to date, no developmental biology and host plant list have been recorded for H. bakeri. Here, we report its life history, conduct surveys, and present potential host plant species. Biological characteristics of H. bakeri reared on Ipomea batatas (L.) Poir. shoots were observed in the laboratory at 27 ± 1 °C, 59 to 73% RH, and L12:D12. Five nymphal stages were recorded. Egg incubation period was 7.49 ± 0.43 days, and the duration of each of the five developmental stages was 3.11 ± 0.53, 1.51 ± 0.37, 2.07 ± 0.11, 4.40 ± 0.32 and 4.45 ± 0.17 days, respectively. Adult longevity was not significantly different between both sexes. We also provided morphological descriptions and measurements of the immature stages to aid proper nymphal identification of H. bakeri. No choice tests and field observations revealed four potential host plants, i.e., Chromolaena odorata (L.) R King & H Robinson, Cucumis sativus L., Hibiscus rosasinensis L., and Psidium guajava L., and two plants, i.e., Polyscias scutellaria (Burm. f.) Fosb. and Ipomea batatas (L.) Poir with confirmed host association. The information gathered on the life history, and host plant associations of H. bakeri would help decide the appropriate pest management strategy for controlling the population of this insect pest in the country's cacao-growing areas.

DOI： 10.1007/s12600-021-00957-1

Web of Science

Scopus

researchmap

Other Link： https://link.springer.com/article/10.1007/s12600-021-00957-1/fulltext.html

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

A morphological and molecular study of 17 Cylindrotomidae species revealed that the two subspecies of <italic>Cylindrotoma distinctissima</italic>, the Nearctic <italic>C. americana</italic> Osten Sacken, 1865, <bold>stat. reval.</bold> and the Palearctic <italic>C. distinctissima</italic> (Meigen, 1818), represent separated lineages and consequently are raised to species level. <italic>Cylindrotoma japonica</italic> Alexander, 1919, <bold>syn. nov</bold>. and <italic>C. distinctissima alpestris</italic> Peus, 1952, <bold>syn. nov.</bold> are now known to be junior synonyms of <italic>C. distinctissima</italic>. <italic>Triogma kuwanai limbinervis</italic> Alexander, 1953, <bold>syn. nov.</bold> and <italic>T. nimbipennis</italic> Alexander, 1941, <bold>syn. nov.</bold> are now placed into synonymy under <italic>Triogma kuwanai</italic> (Alexander, 1913). The Japanese Cylindrotomidae are all redescribed and all available literature and distribution data are summarised. Supplementary descriptions and illustrations for male and female terminalia of <italic>Cylindrotoma nigriventris</italic> Loew, 1849, <italic>Diogma dmitrii</italic> Paramonov, 2005, <italic>Liogma nodicornis</italic> (Osten Sacken, 1865), <italic>Phalacrocera replicata</italic> (Linnaeus, 1758), <italic>P. tipulina</italic> Osten Sacken, 1865, and <italic>Triogma trisulcata</italic> (Schummel, 1829) are provided. The following new distribution records are outlined; <italic>Diogma caudata</italic> Takahashi, 1960 from Arkhangelsk Oblast, Russia; <italic>D. glabrata</italic> (Meigen, 1818) from Belarus, Latvia, and Altai Republic, Amur Oblast, Novgorod Oblast, Magadan Oblast, Samara Oblast, and Kuril Islands (Shikotan I and Paramushir I) in Russia; <italic>Liogma serraticornis</italic> Alexander, 1919 from Khabarovsk Krai, Russia; <italic>Phalacrocera replicata</italic> from Khabarovsk Krai, Russia; and the presence of <italic>Cylindrotoma nigriventris</italic> in Altai Republic, Russia is confirmed.

DOI： 10.3897/zookeys.1083.75624

Scopus

researchmap

Other Link： https://zookeys.pensoft.net/article/75624/download/xml/

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title><sec>
<title>Background</title>
Sequential membrane filtration as a pre-processing step for capturing sediment-associated microorganisms could provide good quality and integrity DNA that can be preserved and kept at ambient temperatures before community profiling through culture-independent molecular techniques. However, the effects of sample pre-processing via filtration on DNA-based profiling of sediment-associated microbial community diversity and composition are poorly understood. Specifically, the influences of pre-processing on the quality and quantity of extracted DNA, high-throughput DNA sequencing reads, and detected microbial taxa need further evaluation.


</sec><sec>
<title>Results</title>
We assessed the impact of pre-processing freshwater sediment samples by sequential membrane filtration (from 10, 5 to 0.22 μm pore size) for 16S rRNA-based community profiling of sediment-associated microorganisms. Specifically, we examined if there would be method-driven differences between non- and pre-processed sediment samples regarding the quality and quantity of extracted DNA, PCR amplicon, resulting high-throughput sequencing reads, microbial diversity, and community composition. We found no significant difference in the qualities and quantities of extracted DNA and PCR amplicons, and the read abundance after bioinformatics processing (i.e., denoising and chimeric-read filtering steps) between the two methods. Although the non- and pre-processed sediment samples had more unique than shared amplicon sequence variants (ASVs), we report that their shared ASVs accounted for 74% of both methods’ absolute read abundance. More so, at the genus level, the final collection filter identified most of the genera (95% of the reads) captured from the non-processed samples, with a total of 51 false-negative (2%) and 59 false-positive genera (3%). We demonstrate that while there were differences in shared and unique taxa, both methods revealed comparable microbial diversity and community composition.


</sec><sec>
<title>Conclusions</title>
Our observations highlight the feasibility of pre-processing sediment samples for community analysis and the need to further assess sampling strategies to help conceptualize appropriate study designs for sediment-associated microbial community profiling.


</sec>

DOI： 10.1186/s12866-022-02441-0

Scopus

PubMed

researchmap

Other Link： https://link.springer.com/article/10.1186/s12866-022-02441-0/fulltext.html

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Magnolia Press  

The larva of the Balkan microendemic Isoperla vevcianensis Ikonomov, 1980 (Plecoptera: Perlodidae: Isoperlinae) is described on the basis of larvae associated with adults by means of cox1 sequences similarities. Eggs and everted penis of the male are described for the first time. The larva possessed blunt paraprocts which were previously sharp among Palaearctic larvae of this huge Holarctic and Oriental genus. The commonly pointed paraproct remains as the only distinguishing generic character for the morphologically diverse larvae of Palaearctic Isoperla, but should not be considered as an exclusive character state for the genus. Morphological characters are illustrated in comparison with the sympatric larvae of the Isoperla tripartita species complex.&#x0D;

DOI： 10.11646/zootaxa.5082.6.2

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/fwb.13824

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/fwb.13824

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.scitotenv.2021.148406

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.meegid.2021.104999

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title><sec>
<title>Background</title>
Production, marketability and consumer preference of red tilapia often depends upon the intensity of coloration. Hence, new approaches to develop coloration are now geared to improve market acceptability and profit. This study evaluated the effects of carotenoid-rich diets on the phenotypic coloration, carotenoid level, weight gain and expression of coloration-linked genes in skin, fin and muscle tissues. Carotenoids were extracted from dried <italic>Daucus carota</italic> peel, <italic>Ipomoea aquatica</italic> leaves, and <italic>Moringa oleifera</italic> leaves. Eighty (80) size-14 fish were fed with carotenoid-rich treatments twice a day for 120 days. The phenotypic effect of the carotenoid extracts was measured through a color chart. Skin carotenoid level was measured through UV-vis spectrophotometer. <italic>csf1ra, Bcdo2</italic> and <italic>StAR</italic> expression analysis was done using qRT-PCR.


</sec><sec>
<title>Results</title>
Treatments with carotenoid extracts yielded higher overall scores on phenotypic coloration and tissue carotenoid levels. Differential expression of carotenoid-linked genes such as the elevated expression in <italic>csf1ra</italic> and lower expression in <italic>Bcdo2b</italic> following supplementation of the enhanced diet supports the phenotypic redness and increased carotenoid values in red tilapia fed with <italic>D. carota</italic> peel and <italic>I. aquatica</italic> leaves.


</sec><sec>
<title>Conclusions</title>
Overall improvement in the redness of the tilapia was achieved through the supplementation of carotenoid-rich diet derived from readily available plants. Differential expression of coloration-linked genes supports the increase in the intensity of phenotypic coloration and level of carotenoids in the tissues. The study emphasizes the importance of carotenoids in the commercial tilapia industry and highlights the potential of the plant extracts for integration and development of feeds for color enhancement in red tilapia.


</sec>

DOI： 10.1186/s12917-021-03006-5

researchmap

Other Link： https://link.springer.com/article/10.1186/s12917-021-03006-5/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/encyclopedia1030067

researchmap

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

Limoniidae is one of the most species-rich Dipteran families, with 661 reported species in Europe. Despite the fact that the European limoniid crane fly fauna has been studied ever since Carolus Linnaeus, it is still poorly known.


In this study, we summarise the taxonomic and faunistic studies of European Limoniidae, which described new species and reported first country records, between 2010 and 2020. We also report occurrence data of 244 Limoniidae species which represent the first country records or conformational records from various European countries, as we report ten species from Albania, one from Austria, thirty-seven from Belarus, five from Belgium, two from Bulgaria, two from Estonia, six from Finland, seven from France, fourteen from Greece, sixteen from Hungary, two from Iceland, six from Italy, ten from Latvia, one from Malta, nine from Montenegro, two from The Netherlands, ten from North Macedonia, forty-two from Norway, one from Poland, five from Portugal, twenty from Romania, thirty-eight from Serbia, six from Slovenia, five from Spain and seven species from Sweden for the first time. From the European territory of Russia, we report twenty-eight species from Central European Russia, seventy-two from East European Russia, fifteen from North European Russia, one from Northwest European Russia and seven from North Caucasus for the first time. Confirmatory records and corrigenda are also included.

DOI： 10.3897/bdj.9.e67085

researchmap

Other Link： https://bdj.pensoft.net/article/67085/download/xml/

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

<italic>Aedes aegypti</italic> is inherently susceptible to arboviruses. The geographical expansion of this vector host species has led to the persistence of Dengue, Zika, and Chikungunya human infections. These viruses take advantage of the mosquito’s cell to create an environment conducive for their growth. Arboviral infection triggers transcriptomic and protein dysregulation in <italic>Ae. aegypti</italic> and in effect, host antiviral mechanisms are compromised. Currently, there are no existing vaccines able to protect human hosts from these infections and thus, vector control strategies such as <italic>Wolbachia</italic> mass release program is regarded as a viable option. Considerable evidence demonstrates how the presence of <italic>Wolbachia</italic> interferes with arboviruses by decreasing host cytoskeletal proteins and lipids essential for arboviral infection. Also, <italic>Wolbachia</italic> strengthens host immunity, cellular regeneration and causes the expression of microRNAs which could potentially be involved in virus inhibition. However, variation in the magnitude of <italic>Wolbachia</italic>’s pathogen blocking effect that is not due to the endosymbiont’s density has been recently reported. Furthermore, the cellular mechanisms involved in this phenotype differs depending on <italic>Wolbachia</italic> strain and host species. This prompts the need to explore the cellular interactions between <italic>Ae. aegypti</italic>-arboviruses-<italic>Wolbachia</italic> and how different <italic>Wolbachia</italic> strains overall affect the mosquito’s cell. Understanding what happens at the cellular and molecular level will provide evidence on the sustainability of <italic>Wolbachia</italic> vector control.

DOI： 10.3389/fcimb.2021.690087

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.toxrep.2021.06.010

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title><sec>
<title>Background</title>
Bandung, the fourth largest city in Indonesia and capital of West Java province, has been considered a major endemic area of dengue, and studies show that the incidence in this city could increase and spread rapidly. At the same time, estimation of incidence could be inaccurate due to a lack of reliable surveillance systems. To provide strategic information for the dengue control program in the face of limited capacity, this study used spatial pattern analysis of a possible outbreak of dengue cases, through the Geographic Information System (GIS). To further enhance the information needed for effective policymaking, we also analyzed the demographic pattern of dengue cases.


</sec><sec>
<title>Methods</title>
Monthly reports of dengue cases from January 2014 to December 2016 from 16 hospitals in Bandung were collected as the database, which consisted of address, sex, age, and code to anonymize the patients. The address was then transformed into geocoding and used to estimate the relative risk of a particular area’s developing a cluster of dengue cases. We used the kernel density estimation method to analyze the dynamics of change of dengue cases.


</sec><sec>
<title>Results</title>
The model showed that the spatial cluster of the relative risk of dengue incidence was relatively unchanged for 3 years. Dengue high-risk areas predominated in the southern and southeastern parts of Bandung, while low-risk areas were found mostly in its western and northeastern regions. The kernel density estimation showed strong cluster groups of dengue cases in the city.


</sec><sec>
<title>Conclusions</title>
This study demonstrated a strong pattern of reported cases related to specific demographic groups (males and children). Furthermore, spatial analysis using GIS also visualized the dynamic development of the aggregation of disease incidence (hotspots) for dengue cases in Bandung. These data may provide strategic information for the planning and design of dengue control programs.


</sec>

DOI： 10.1186/s41182-021-00329-9

researchmap

Other Link： https://link.springer.com/article/10.1186/s41182-021-00329-9/fulltext.html

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

The virulence and drug resistance of globally prevalent <italic>Candida albicans</italic> has presented complications toward its control while advances in effective antivirulence drugs remain critical. Emerging methods are now being evaluated to facilitate development of novel therapeutic approaches against this pathogen. This study focuses on the biofilm formation inhibition of ethnobotanical crude extracts and the use of nanotechnology through the ethnobotanically-synthesized gold nanoparticles to control <italic>C. albicans</italic>. Control on biofilm formation was compared using crude extracts (CEs) and biologically synthesized gold nanoparticles (CEs + AuNPs). Significantly lower biofilm formation was exhibited in thirteen (13) CEs and fourteen (14) CEs + AuNPs. Biofilm-linked genes <italic>Bcr1</italic> and <italic>HSP90</italic> expression were consequently downregulated. Higher biofilm inhibition activity was noted in some CEs + AuNPs compared to its counterpart CEs. This study emphasizes the biofilm inhibition activity of ethnobotanicals and the use of nanoparticles to enhance delivery of compounds, and points to its prospects for developing anti-pathogenic drugs without evolving resistance.

DOI： 10.3389/fmicb.2021.665113

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3897/BDJ.9.e58009

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.ttbdis.2021.101683

researchmap

Publishing type：Research paper (scientific journal)  

[This corrects the article DOI: 10.2196/19034.].

DOI： 10.2196/29795

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JMIR Publications Inc.  

<sec>
<title>Background</title>
While early detection and effective control of epidemics depend on appropriate surveillance methods, the Philippines bases its dengue fever surveillance system on a passive surveillance method (notifications from barangay/village health centers, municipal or city health offices, hospitals, and clinics). There is no available mHealth (mobile health) app for dengue fever that includes all the appropriate surveillance methods in early detection of disease outbreaks in the country.


</sec>
<sec>
<title>Objective</title>
This study aimed to evaluate the usability of the Mozzify app in terms of objective quality (engagement, functionality, aesthetics, information) and app subjective and app-specific qualities and compare total app mean score ratings by sociodemographic profile and self and family dengue fever history to see what factors are associated with high app mean score rating among school-based young adult samples and health care professionals. Individual interviews and focus group discussions were also conducted among participants to develop themes from their comments and suggestions to help structure further improvement and future development of the app.


</sec>
<sec>
<title>Methods</title>
User experience sessions were conducted among participants, and the Mobile Application Rating Scale (MARS) professional and user versions (uMARS) were administered followed by individual interviews and focus group discussions. Descriptive statistical analysis of the MARS and uMARS score ratings was performed. The total app mean score ratings by sociodemographic and dengue fever history using nonparametric mean difference analyses were also conducted. Thematic synthesis was used to develop themes from the comments and suggestions raised in individual interviews and focus group discussions.


</sec>
<sec>
<title>Results</title>
Mozzify obtained an overall &gt;4 (out of 5) mean score ratings in the MARS and uMARS app objective quality (4.45), subjective (4.17), and specific (4.55) scales among 948 participants (79 health care professionals and 869 school-based samples). Mean difference analyses revealed that total app mean score ratings were not significantly different across ages and gender among health care professionals and across age, income categories, and self and family dengue fever history but not gender (P&lt;.001) among the school-based samples. Thematic syntheses revealed 7 major themes: multilanguage options and including other diseases; Android version availability; improvements on the app’s content, design, and engagement; inclusion of users from low-income and rural areas; Wi-Fi connection and app size concerns; data credibility and issues regarding user security and privacy.


</sec>
<sec>
<title>Conclusions</title>
With its acceptable performance as perceived by health care professionals and school-based young adults, Mozzify has the potential to be used as a strategic health intervention system for early detection of disease outbreaks in the Philippines. It can be used by health care professionals of any age and gender and by school-based samples of any age, socioeconomic status, and dengue fever history. The study also highlights the feasibility of school-based young adults to use health-related apps for disease prevention.


</sec>

DOI： 10.2196/19034

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

DOI： 10.1371/journal.pntd.0009139

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title>Japanese spotted fever, a tick-borne disease caused by <italic>Rickettsia japonica</italic>, was firstly described in southwestern Japan. There was a suspicion of <italic>Rickettsia japonica</italic> infected ticks reaching the non-endemic Niigata Prefecture after a confirmed case of Japanese spotted fever in July 2014. Therefore, from 2015 to 2017, 38 sites were surveyed and rickettsial pathogens were investigated in ticks from north to south of Niigata Prefecture including Sado island. A total of 3336 ticks were collected and identified revealing ticks of three genera and ten species: <italic>Dermacentor taiwanensis</italic>, <italic>Haemaphysalis flava</italic>, <italic>Haemaphysalis hystricis</italic>, <italic>Haemaphysalis longicornis</italic>, <italic>Haemaphysalis megaspinosa</italic>, <italic>Ixodes columnae</italic>, <italic>Ixodes monospinosus</italic>, <italic>Ixodes nipponensis</italic>, <italic>Ixodes ovatus,</italic> and <italic>Ixodes persulcatus</italic>. Investigation of rickettsial DNA showed no ticks infected by <italic>R. japonica</italic>. However, three species of spotted fever group rickettsiae (SFGR) were found in ticks, <italic>R. asiatica</italic>, <italic>R. helvetica,</italic> and <italic>R. monacensis</italic>, confirming Niigata Prefecture as a new endemic area to SFGR. These results highlight the need for public awareness of the occurrence of this tick-borne disease, which necessitates the establishment of public health initiatives to mitigate its spread.

DOI： 10.1038/s41598-021-81587-9

researchmap

Other Link： http://www.nature.com/articles/s41598-021-81587-9

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Comperiella calauanica is a host-specific endoparasitoid and effective biological control agent of the diaspidid Aspidiotus rigidus, whose outbreak from 2010 to 2015 severely threatened the coconut industry in the Philippines. Using the maximum entropy (Maxent) algorithm, we developed a species distribution model (SDM) for C. calauanica based on 19 bioclimatic variables, using occurrence data obtained mostly from field surveys conducted in A. rigidus-infested areas in Luzon Island from 2014 to 2016. The calculated the area under the ROC curve (AUC) values for the model were very high (0.966, standard deviation = 0.005), indicating the model’s high predictive power. Precipitation seasonality was found to have the highest relative contribution to model development. Response curves produced by Maxent suggested the positive influence of mean temperature of the driest quarter, and negative influence of precipitation of the driest and coldest quarters on habitat suitability. Given that C. calauanica has been found to always occur with A. rigidus in Luzon Island due to high host-specificity, the SDM for the parasitoid may also be considered and used as a predictive model for its host. This was confirmed through field surveys conducted between late 2016 and early 2018, which found and confirmed the occurrence of A. rigidus in three areas predicted by the SDM to have moderate to high habitat suitability or probability of occurrence of C. calauanica: Zamboanga City in Mindanao; Isabela City in Basilan Island; and Tablas Island in Romblon. This validation in the field demonstrated the utility of the bioclimate-based SDM for C. calauanica in predicting habitat suitability or probability of occurrence of A. rigidus in the Philippines.

DOI： 10.3390/insects12010026

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.meegid.2020.104676

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

Japanese species of the genus <italic>Nipponomyia</italic> Alexander, 1924 are revised. Two new species, <italic>Nipponomyia okinawensis</italic> Kolcsár &amp;amp; Kato, <bold>sp. nov.</bold> and <italic>N. yakushimensis</italic> Kolcsár &amp;amp; Kato, <bold>sp. nov.</bold> are described from the Ryukyu Islands. Images of habitus and wings, illustrations of male and female terminalia, and distribution maps are provided for the Japanese species. A key to the world species of <italic>Nipponomyia</italic> is added. DNA barcodes of three Japanese <italic>Nipponomyia</italic> are provided, representing the first barcodes from the genus.

DOI： 10.3897/zookeys.1000.55021

Scopus

researchmap

Other Link： https://zookeys.pensoft.net/article/55021/download/xml/

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/ddi.13160

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ddi.13160

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/ecog.05118

Scopus

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ecog.05118

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

Japanese species of the genus <italic>Ulomorpha</italic> Osten Sacken, 1869 are revised and <italic>U. amamiana</italic> Kato &amp;amp; Kolcsár, <bold>sp. nov.</bold> and <italic>U. longipenis</italic> Kato &amp;amp; Kolcsár, <bold>sp. nov.</bold> are described. A key to the four Japanese species of the genus is provided, with images of habitus and wings, and drawings of their male terminalia. <italic>Ulomorpha amamiana</italic> Kato &amp;amp; Kolcsár, <bold>sp. nov.</bold> is the first representative of the genus discovered from the Oriental region.

DOI： 10.3897/zookeys.999.52831

Scopus

researchmap

Other Link： https://zookeys.pensoft.net/article/52831/download/xml/

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title><sec>
<title>Background</title>
Vector control measures are critical for the prevention and reduction of dengue virus (DENV) transmission. Effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito-borne infection. Mosquito-based virus surveillance programs typically rely on pool-based mosquito testing, although whether individual-based mosquito testing is a feasible alternative to this has not been widely studied. Applying an individual-based mosquito testing approach, we conducted a 1-month surveillance study of DENV in adult <italic>Aedes aegypti</italic> mosquitoes in homes of suspected dengue patients during the 2015 peak dengue season in Tarlac City, Philippines to more accurately assess the mosquito infection rate and identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients there.


</sec><sec>
<title>Methods</title>
We performed a one-step multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and serotyping of DENV in patients and individual female <italic>Ae. aegypti</italic> mosquitoes. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENV serotypes in mosquitoes and patients at the genotype level.


</sec><sec>
<title>Results</title>
We collected a total of 583 adult <italic>Ae. aegypti</italic> mosquitoes, of which we individually tested 359 female mosquitoes for the presence of DENV. Ten (2.8%) of the 359 female mosquitoes were positive for the presence of DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which was consistent with the serotypes concurrently found in infected patients. Sequencing and phylogenetic analyses of the detected DENV serotypes based on the partial sequence of the evelope (<italic>E</italic>) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely DENV-1 genotype IV, DENV-2 Cosmopolitan genotype, and DENV-4 genotype II.


</sec><sec>
<title>Conclusions</title>
We demonstrated the utility of a one-step multiplex real-time RT-PCR assay for the individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which are critical for dengue prevention and control.


</sec>

DOI： 10.1186/s13071-020-04470-y

Scopus

PubMed

researchmap

Other Link： http://link.springer.com/article/10.1186/s13071-020-04470-y/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

The coconut scale insect, Aspidiotus rigidus Reyne, caused a major pest outbreak in coconut plantations and stands in the Southern Tagalog region of Luzon Island in the Philippines between 2010 and 2015. To determine if parasitism by Comperiella calauanica Barrion, Almarinez and Amalin, a native encyrtid, could have been a factor in the eventual management of the outbreak by 2015, we estimated and assessed its parasitization levels on A. rigidus colonies on field-collected samples from selected points in three provinces in the Southern Tagalog Region across three sampling periods. We observed that C. calauanica consistently occurred only in areas where A. rigidus populations occurred, with high parasitization levels in the Southern Tagalog sites from 2014 to 2015. Results of correlation and regression of total scale count against parasitized scale count suggest putative host density-dependent parasitism by C. calauanica in the field. A marked decrease in the abundance of A. rigidus was recorded concurrently with visually observable recovery of coconut trees from the third quarter of 2014 up to the second quarter of 2016. Similar results of significant reduction in A. rigidus populations concurrent with high percent parasitization by mass-reared and released C. calauanica were found in the Zamboanga Peninsula from 2018 to 2020. Our findings and observations altogether suggest that host-specific parasitization by C. calauanica effected biological control, which may have contributed to the eventual management of the A. rigidus outbreak in the Southern Tagalog Region, and also in the Zamboanga Peninsula where similar recovery of coconut trees were observed within a year after inoculative releases of C. calauanica.

DOI： 10.3390/insects11110745

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/ecy.3127

Scopus

PubMed

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ecy.3127

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

The black locust Robinia pseudoacacia (Robinieae, Fabaceae) is a common invasive riparian tree in Japan. There are less effective management strategies to remove the tree from the riparian area because of its quickly established high population. We investigated the expansion patterns of R. pseudoacacia through sympatric (i.e. between high- and low-water channel (HWC/LWC) within a study site) and allopatric (i.e. along river corridor) dispersal in the Tama River (Tokyo, Japan). Four microsatellites were used to examine the effects of gene flow on six populations in three sites. These subpopulations showed small genetic distance (i.e. no barrier or slightly limited) and genetically mixed population structure. It indicated that both sympatric and allopatric dispersals were active. Many migrants were younger individuals (i.e. <5 years old) and were found in the LWC area. Thus, the LWC could receive more migrants than the HWC through both types of dispersals. In addition, our age and genetic structure analyses reveal that recruited individuals likely settled immediately after the clearing project of R. pseudoacacia through sympatric dispersal. It appears that the migration by allopatric dispersal occurred following this. For the effective management of R. pseudoacacia, migrants should be removed regularly following initial removal of invaders during site restoration.

DOI： 10.1093/aobpla/plaa043

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/bes2.1767

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bes2.1767

Authorship：Last author   Publishing type：Research paper (scientific journal)  

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. The inevitable occurrence of diseases caused by bacterial pathogens is one of the main constraints in tilapia industry that has resulted in major economic losses. The study reveals the advantages of quorum sensing inhibition through nanotechnology in developing antivirulence drugs to control aquaculture pathogens using scientifically untapped medicinal plants, the ethnobotanicals. The ethnobotanical crude extracts (CEs) and biologically synthesized gold nanoparticles (CEs + AuNPs) of the Ilongot-Eǵongot community exhibit quorum sensing inhibition (QSI) activity through inhibition of the biofilm formation against gram-positive Streptococcus agalactiae. Furthermore, ethnobotanical CEs + AuNPs show much greater activity than its counterpart CEs in antibacterial and biofilm formation assay in S. agalactiae which is molecularly confirmed by gene expression analysis. The results indicate the potential of these ethnobotanicals for therapeutic approach in which it showed minimal expression of agrA gene linked in biofilm formation and connotes maximal inhibition of QS in S. agalactiae thereby can possibly inhibit bacterial resistance and virulence.

DOI： 10.1007/s12668-020-00758-6

Scopus

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Adaptive divergence is a key mechanism shaping the genetic variation of natural populations. A central question linking ecology with evolutionary biology is how spatial environmental heterogeneity can lead to adaptive divergence among local populations within a species. In this study, using a genome scan approach to detect candidate loci under selection, we examined adaptive divergence of the stream mayfly Ephemera strigata in the Natori River Basin in northeastern Japan. We applied a new machine-learning method (i.e., random forest) besides traditional distance-based redundancy analysis (dbRDA) to examine relationships between environmental factors and adaptive divergence at non-neutral loci. Spatial autocorrelation analysis based on neutral loci was employed to examine the dispersal ability of this species. We conclude the following: (a) E. strigata show altitudinal adaptive divergence among the populations in the Natori River Basin; (b) random forest showed higher resolution for detecting adaptive divergence than traditional statistical analysis; and (c) separating all markers into neutral and non-neutral loci could provide full insight into parameters such as genetic diversity, local adaptation, and dispersal ability.

DOI： 10.1002/ece3.6398

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

Dengue is a highly endemic disease in Southeast Asia and is transmitted primarily by the mosquito, Aedes aegypti. The National Capital Region (NCR) of the Philippines, or Metropolitan Manila, is a highly urbanized area that is greatly affected by this arboviral disease. Urbanization has been shown to increase the dispersal of this mosquito vector. For this reason, we conducted a fine-scale population genetic study of Ae. aegypti in this region. We collected adult Ae. aegypti mosquitoes (n = 526 individuals) within the region (n = 21 study areas) and characterized the present population structure and the genetic relatedness among mosquito populations. We genotyped 11 microsatellite loci from all sampled mosquito individuals and analyzed their genetic diversity, differentiation and structure. The results revealed low genetic differentiation across mosquito populations which suggest high gene flow and/or weak genetic drift among mosquito populations. Bayesian analysis indicated multiple genetic structures (K = 3-6), with no clear genetically distinct population structures. This result implies the passive or long-distance dispersal capability nature Ae. aegypti possibly through human-mediated transportation. The constructed dendrogram in this study describes the potential passive dispersal patterns across Metropolitan Manila. Furthermore, spatial autocorrelation analysis showed the limited and active dispersal capability (<1km) of the mosquito vector. Our findings are consistent with previous studies that investigated the genetic structure and dual (active and passive) dispersal capability of Ae. aegypti in a fine-scale highly urbanized area.

DOI： 10.1371/journal.pntd.0008279

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

DOI： 10.1371/journal.pone.0229120

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This study was performed to evaluate the health information system regarding the dengue surveillance system in Indonesia. Major obstacles to the implementation of an effective health information system regarding dengue cases in Bandung are examined, and practical suggestions on measures to overcome them are discussed. The study utilized a mixed-method research design using qualitative approaches: document analysis, key informants and focus group interviews. Thirty key informants were selected, comprised of policymakers, senior managers, and staff at the Ministry of Health. Data from documents and transcripts were evaluated through a modified Institutional Analysis and Development (IAD) framework described by Ostrom. Through this study, we have identified several issues that hinder the effective implementation of the health information system in the case of dengue in Bandung. In the end, we propose several recommendations for reform that encompasses motivational, strategic, and structural approaches to each component of the analysis. Through evaluation of the health information system for dengue surveillance in Indonesia, we conclude that well-coordination in multi-level governance in a country as large as Indonesia is the key in the implementation of the health information system in different levels of agencies. Furthermore, the adaptability of human resources in adopting a new information system also plays an important part.

DOI： 10.3390/ijerph17051795

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JMIR Publications Inc.  

<sec>
<title>Background</title>
For the last 10 years, mobile phones have provided the global health community with innovative and cost-effective strategies to address the challenges in the prevention and management of dengue fever.


</sec>
<sec>
<title>Objective</title>
The aim is to introduce and describe the design and development process of Mozzify, an integrated mobile health (mHealth) app that features real-time dengue fever case reporting and mapping system, health communication (real-time worldwide news and chat forum/timeline, within-app educational videos, links to local and international health agency websites, interactive signs and symptoms checker, and a hospital directions system), and behavior modification (reminders alert program on the preventive practices against dengue fever). We also aim to assess Mozzify in terms of engagement and information-sharing abilities, functionality, aesthetics, subjective quality, and perceived impact.


</sec>
<sec>
<title>Methods</title>
The main goals of the Mozzify app were to increase awareness, improve knowledge, and change attitudes about dengue fever, health care-seeking behavior, and intention-to-change behavior on preventive practices for dengue fever among users. It was assessed using the Mobile Application Rating Scale (MARS) among 50 purposively sampled individuals: public health experts (n=5), environment and health-related researchers (n=23), and nonclinical (end users) participants (n=22).


</sec>
<sec>
<title>Results</title>
High acceptability and excellent satisfaction ratings (mean scores ≥4.0 out of 5) based on the MARS subscales indicate that the app has excellent user design, functionality, usability, engagement, and information among public health experts, environment and health-related researchers, and end users. The app’s subjective quality (recommending the app to other people and the app’s overall star rating), and specific quality (increase awareness, improve knowledge, and change attitudes about dengue fever; health care-seeking behavior; and intention-to-change behavior on preventive practices for dengue fever) also obtained excellent satisfaction ratings from the participants. Some issues and suggestions were raised during the focus group and individual discussions regarding the availability of the app for Android devices, language options limitations, provision of predictive surveillance, and inclusion of other mosquito-borne diseases.


</sec>
<sec>
<title>Conclusions</title>
Mozzify may be a promising integrated strategic health intervention system for dengue fever case reporting and mapping; increase awareness, improve knowledge, and change attitude about dengue fever; and disseminating and sharing information on dengue fever among the general population and health experts. It also can be an effective aid in the successful translation of knowledge on preventive measures against dengue fever to practice.


</sec>

DOI： 10.2196/16424

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

Copyright © 2020 Magnolia Press A new species of the genus Protonemura Kempny, 1898 (Plecoptera: Nemouridae: Amphinemurinae) is described from the Ishizuchi Range of Shikoku Island, Japan, based on the male, female and Co1 sequences. Protonemura shimizui Murányi & Gamboa sp. n. belongs to the P. orbiculata species group sensu Shimizu, 1998. The new species appears to be limited to the higher elevations of the Ishizuchi Range. Faunistic survey of the high elevation rheocrenes of the Ishizuchi Range is also presented.

DOI： 10.11646/zootaxa.4718.1.4

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Background: Psychiatric symptoms have been reported in adult patients with dengue fever (DF); however, information on pediatric patients remains inadequate. We sought to identify the prevalence and predictors of depressive and anxiety symptoms and identify other psychiatric symptoms among pediatric patients with DF. This case-control study involved pediatric in-patients (n = 225) who had clinical or serologic-confirmed DF and healthy school-based controls (n = 260). Participants completed the Revised Child Anxiety and Depression Scale (RCADS). Results: The prevalence of depressive (13.3%) and anxiety (34.2%) symptoms among pediatric patients with DF was significantly (p < 0.001) higher than that among controls (3.5% and 16.2%, respectively). Multiple linear regression analysis found that age, family history of DF, ≤2 days of hospitalization, myalgia, and arthralgia were predictors of increased depressive and anxiety symptoms among the patients. Further, 26.7% of pediatric patients reported irritability, agitation, visual hallucinations, and aggressiveness. Conclusion: Pediatric patients present depressive and anxiety symptoms whose levels were associated with social and clinical factors. However, whether these symptoms are present only during the infection or may still persist after recovery or are brought by children’s adverse reactions to hospitalization are unknown, and thus, further studies are needed.

DOI： 10.3390/ijerph17010099

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Background: Knowledge, attitude, and practice (KAP) of in-patients with dengue fever (DF) through hospital-based surveillance has not been done. This study aimed to assess and compare the KAP, identify its predictors, correlation, and protective factors among pediatric and adult patients with DF and community-based controls to structure proactive community-wide DF prevention and control programs. Methods: This case-control study involved clinically or serologically confirmed patients (pediatrics n = 233; adults n = 17) with DF admitted in three public hospitals and community-based controls in Metro Manila, Philippines. A pretested structured KAP questionnaire was administered to participants to assess their KAP. Results: Pediatric and adult patients had significantly lower mean scores in the practice (p < 0.001) domain compared with the pediatric and adult controls. Being in senior high school, having had days in hospital, and rash were predictors of KAP among pediatric patients. Knowledge and attitude of patients with DF did not correlate with their practices against DF. Use of mosquito-eating fish, screen windows, and dengue vaccine were protective factors against DF. Conclusion: The study highlights the importance of behavioral change for knowledge and attitude to have significant effect to practices against DF. Thus, we recommend two comprehensive health programs, Communication for Behavioral Impact (COMBI) and Health Belief Model (HBM).

DOI： 10.3390/ijerph16234705

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Despite the fact that massive outbreaks of the coconut scale insect pest, Aspidiotus rigidus Reyne (Hemiptera: Diaspididae) are inflicting significant economic losses to the Philippines' coconut industry, little is known about the population and dispersal history of this invasive pest in the country. Here, we examined the genetic diversity, structure and demographic history of A. rigidus sampled from localities with reported outbreaks from 2014 to 2017. We analyzed the genetic structure of the outbreak populations using mitochondrial COI and nuclear EF-1α markers. Both markers and all methods of population genetic structure analyses indicate clear differentiation among the A. rigidus populations separating the north from the southern regions of the Philippines. Very low or no genetic differentiation was observed within and amongst the populations per geographic region indicating two unrelated outbreak events of the pest originating from two genetically uniform populations isolated in each respective region. Historical data supports the resurgence of an established A. rigidus population in the south which could have been driven by sudden climatic changes or human-induced habitat imbalance. Our study provides valuable information on the genetic differentiation of the two A. rigidus groups that would be useful for developing and implementing biological control strategies against this pest in the Philippines.

DOI： 10.3390/insects10110374

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Recent reports reveal the presence of Wolbachia in Ae. aegypti. Our study presents additional support for Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using two Wolbachia-specific molecular makers. METHODS: A total of 672 Ae. aegypti adult mosquitoes were collected from May 2014 to January 2015 in Metropolitan Manila. Each individual sample was processed and screened for the presence of Wolbachia by selected markers, Wolbachia-specific 16S rDNA and its surface protein (wsp), under optimized PCR conditions and sequenced. RESULTS: Totals of 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A and B while the majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples showed positive amplifications in both markers and 69% showed congruence in supergroup identification (supergroup B). CONCLUSIONS: By utilizing two Wolbachia-specific molecular makers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low Wolbachia infection rate and inferred the detected strains belong to either supergroups A and B.

DOI： 10.1186/s13071-019-3629-y

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JCDR Research and Publications  

DOI： 10.7860/JCDR/2019/41059.13004

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER  

Conventional morphology-based identification is commonly used for routine assessment of freshwater ecosystems. However, cost and time efficient techniques such as high-throughput sequencing (HTS) based approaches may resolve the constraints encountered in conducting morphology-based surveys. Here, we characterized stream macroinvertebrate species diversity and community composition via metabarcoding and morphological analysis from environmental samples collected from the Shigenobu River Basin in Ehime Prefecture, Japan. We compared diversity metrics and assessed both approaches' ability to evaluate the relationship between macroinvertebrate community and environmental variables. In total, we morphologically identified 45 taxa (3 families, six subfamilies, 31 genera, and five species) from 8,276 collected individuals from ten study sites. We detected 44 species by metabarcoding, with 35 species collapsed into 11 groups matching the morphologically identified taxa. A significant positive correlation between logged depth (number of HTS reads) and abundance of morphological taxa was observed, which implied that quantitative data could be used for subsequent analyses. We recovered a considerably high rate of relative abundance detection of the morphologically identified samples. Recovery of samples by incidence or presence/absence count were considerably low, with a high rate of false-negative detection specifically for species with scarce representation in the community sample. Given the low taxonomic resolution of the morphological assignment in this study, we report that metabarcoding does not reflect the majority of the species naturally occurring in our site, which could further be proven by performing refined morphological assessment of the samples. However, abundance-based detection proved to be efficient with 92% of the individuals correctly demonstrated. We conclude that DNA metabarcoding provides a practical and cost-effective approach especially for rapid biological monitoring of freshwater macroinvertebrate communities, but further improvements in the detection of scarce samples should be considered to increase the sensitivity of detecting most, if not all, of the species present in the environment.

DOI： 10.1016/j.ecolind.2019.02.008

Web of Science

Scopus

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2019 The Author(s). Background: Environmental heterogeneity continuously produces a selective pressure that results in genomic variation among organisms; understanding this relationship remains a challenge in evolutionary biology. Here, we evaluated the degree of genome-environmental association of seven stonefly species across a wide geographic area in Japan and additionally identified putative environmental drivers and their effect on co-existing multiple stonefly species. Double-digest restriction-associated DNA (ddRAD) libraries were independently sequenced for 219 individuals from 23 sites across four geographical regions along a nationwide latitudinal gradient in Japan. Results: A total of 4251 candidate single nucleotide polymorphisms (SNPs) strongly associated with local adaptation were discovered using Latent mixed models; of these, 294 SNPs showed strong correlation with environmental variables, specifically precipitation and altitude, using distance-based redundancy analysis. Genome-genome comparison among the seven species revealed a high sequence similarity of candidate SNPs within a geographical region, suggesting the occurrence of a parallel evolution process. Conclusions: Our results revealed genomic signatures of local adaptation and their influence on multiple, co-occurring species. These results can be potentially applied for future studies on river management and climatic stressor impacts.

DOI： 10.1186/s12864-019-5453-3

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

© 2019 Gamboa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The generation of the high species diversity of insects in Japan was profoundly influenced by the formation of the Japanese Archipelago. We explored the species diversification and biogeographical history of the Nemouridae Billberg, 1820 family in the Japanese Archipelago using mitochondrial DNA and nuclear DNA markers. We collected 49 species among four genera: Indonemoura Baumann, 1975; Protonemura Kempny, 1898; Amphinemura, Ris 1902 and Nemoura Latreille, 1796 in Japan, China, South Korea and North America. We estimated their divergence times—based on three molecular clock node calibrations—using Bayesian phylogeography approaches. Our results suggested that Japanese Archipelago formation events resulted in diversification events in the middle of the Cretaceous (<120 Ma), speciation in the Paleogene (<50 Ma) and intra-species diversification segregated into eastern and western Japan of the Fossa Magna region at late Neogene (20 Ma). The Indonemoura samples were genetically separated into two clades—that of Mainland China and that of Japan. The Japanese clade clustered with the Nemouridae species from North America, suggesting the possibility of a colonisation event prior to the formation of the Japanese Archipelago. We believe that our results enhanced the understanding both of the origin of the species and of local species distribution in the Japanese Archipelago.

DOI： 10.1371/journal.pone.0210269

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

© 2018 The Author(s). Sediment bypass tunnels (SBTs) are guiding structures used to reduce sediment accumulation in reservoirs during high flows by transporting sediments to downstream reaches during operation. Previous studies monitoring the ecological effects of SBT operations on downstream reaches suggest a positive influence of SBTs on riverbed sediment conditions and macroinvertebrate communities based on traditional morphology-based surveys. Morphology-based macroinvertebrate assessments are costly and time-consuming, and the large number of morphologically cryptic, small-sized and undescribed species usually results in coarse taxonomic identification. Here, we used DNA metabarcoding analysis to assess the influence of SBT operations on macroinvertebrates downstream of SBT outlets by estimating species diversity and pairwise community dissimilarity between upstream and downstream locations in dam-fragmented rivers with operational SBTs in comparison to dam-fragmented (i.e., no SBTs) and free-flowing rivers (i.e., no dam). We found that macroinvertebrate community dissimilarity decreases with increasing operation time and frequency of SBTs. These factors of SBT operation influence changes in riverbed features, e.g. sediment relations, that subsequently effect the recovery of downstream macroinvertebrate communities to their respective upstream communities. Macroinvertebrate abundance using morphologically-identified specimens was positively correlated to read abundance using metabarcoding. This supports and reinforces the use of quantitative estimates for diversity analysis with metabarcoding data.

DOI： 10.1038/s41598-018-28624-2

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2018 E-Flow Wolters Kluwer Medknow Publications. All rights reserved. Background & objectives: Culex quinquefasciatus is a peridomestic mosquito known for its ability to transmit pathogenic diseases such as filariasis and Japanese encephalitis. The development and use of novel and innovative vector control measures such as the utilization of Wolbachia, along with the existing ones, are necessary to prevent the transmission of these diseases. Studies exploring the diversity of Wolbachia, particularly in Cx. quinquefasciatus are very limited in the Philippines. Thus, the aim of the study was to detect the presence, distribution, and phylogenetic relationship of Wolbachia infections in Cx. quinquefasciatus in Metropolitan Manila, Philippines. Methods: Adult Cx. quinquefasciatus mosquitoes were collected using a commercially available light-trap from May 2014-January 2015. Based on their sampling grids (n = 51), the adult mosquito abdomens were pooled and subjected to Wolbachia surface protein (wsp) gene amplification assay. Five selected wsp-positive samples were then sequenced and further analyzed to infer their phylogenetic relationship with known Wolbachia strains. Results: A total of 1090 adult Cx. quinquefasciatus mosquitoes were collected. Pooled abdomens (n = 53) were then sorted based on their sampling grids for subsequent screening of wsp gene. Wolbachia infection rate was 59% (31/53). These infections were located at 29 (57%) sampling grids, and were observed to be widely distributed in the study area. Phylogenetic analysis indicated that the sample sequences were Wolbachia pipientis isolated from known hosts, Cx. pipiens and Cx. quinquefasciatus belonging to supergroup B clade. Interpretation & conclusion: The study was able to demonstrate the prevalence and distribution of Wolbachia in Cx. quinquefasciatus in Metropolitan Manila, Philippines. The findings of this study are geared towards proposing a vector control program that utilizes the potential of Wolbachia as a biological control agent in preventing the transmission of Culex-borne diseases.

DOI： 10.4103/0972-9062.256561

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

© 2018 by the authors. Dengue is a major public health concern and an economic burden in the Philippines. Despite the country's improved dengue surveillance, it still suffers from various setbacks and needs to be complemented with alternative approaches. Previous studies have demonstrated the potential of Internet-based surveillance such as Google Dengue Trends (GDT) in supplementing current epidemiological methods for predicting future dengue outbreaks and patterns. With this, our study has two objectives: (1) assess the temporal relationship of weekly GDT and dengue incidence in Metropolitan Manila from 2009–2014; and (2) examine the health-seeking behavior based on dengue-related search queries of the population. The study collated the population statistics and reported dengue cases in Metropolitan Manila from respective government agencies to calculate the dengue incidence (DI) on a weekly basis for the entire region and annually per city. Data processing of GDT and dengue incidence was performed by conducting an ‘adjustment' and scaling procedures, respectively, and further analyzed for correlation and cross-correlation analyses using Pearson's correlation. The relative search volume of the term ‘dengue' and top dengue-related search queries in Metropolitan Manila were obtained and organized from the Google Trends platform. Afterwards, a thematic analysis was employed, and word clouds were generated to examine the health behavior of the population. Results showed that weekly temporal GDT pattern are closely similar to the weekly DI pattern in Metropolitan Manila. Further analysis showed that GDT has a moderate and positive association with DI when adjusted or scaled, respectively. Cross-correlation analysis revealed a delayed effect where GDT leads DI by 1–2 weeks. Thematic analysis of dengue-related search queries indicated 5 categories namely; (a) dengue, (b) sign and symptoms of dengue, (c) treatment and prevention, (d) mosquito, and (e) other diseases. The majority of the search queries were classified in ‘signs and symptoms' which indicate the health-seeking behavior of the population towards the disease. Therefore, GDT can be utilized to complement traditional disease surveillance methods combined with other factors that could potentially identify dengue hotspots and help in public health decisions.

DOI： 10.3390/tropicalmed3040118

Scopus

PubMed

researchmap

Authorship：Last author   Language：Japanese   Publishing type：Research paper (scientific journal)  

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

© 2018 American Society for Microbiology. Human noroviruses are excreted in feces from infected individuals and included in wastewater. It is critical to remove/inactivate them in wastewater treatment processes, particularly in the disinfection step, before release to aquatic environments. However, the high mutation rates of human noroviruses raise concerns about the emergence of strains that are less susceptible to disinfectants and can survive even after wastewater treatment. This study aimed to demonstrate the strain-dependent susceptibility of norovirus to free chlorine. A population originated from the murine norovirus strain S7-PP3, a surrogate for human noroviruses in environmental testing, was exposed to free chlorine and then propagated in a host cell. This cycle of free chlorine exposure followed by propagation in cells was repeated 10 times, and populations with lower susceptibility to free chlorine were obtained from two independent trials of chlorine exposure cycles. Open reading frame 2 (ORF2) and ORF3 of the murine norovirus genome were analyzed by next-generation sequencing, and a unique nonsynonymous mutation (corresponding to a change from phenylalanine to serine) at nucleotide (nt) 7280 in ORF3, which encodes the minor capsid protein VP2, was found in chlorine-exposed populations from both trials. It was confirmed that all of the clones from the chlorine-treated population had lower susceptibility to free chlorine than those from the control population. These results indicate that exposure to free chlorine and dilution exert different driving forces to form murine norovirus (MNV) quasispecies, and that there is a selective force to form MNV quasispecies under free chlorine exposure.

DOI： 10.1128/AEM.00244-18

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2018 The Author(s). Background: Several studies have applied ecological factors such as meteorological variables to develop models and accurately predict the temporal pattern of dengue incidence or occurrence. With the vast amount of studies that investigated this premise, the modeling approaches differ from each study and only use a single statistical technique. It raises the question of whether which technique would be robust and reliable. Hence, our study aims to compare the predictive accuracy of the temporal pattern of Dengue incidence in Metropolitan Manila as influenced by meteorological factors from four modeling techniques, (a) General Additive Modeling, (b) Seasonal Autoregressive Integrated Moving Average with exogenous variables (c) Random Forest and (d) Gradient Boosting. Methods: Dengue incidence and meteorological data (flood, precipitation, temperature, southern oscillation index, relative humidity, wind speed and direction) of Metropolitan Manila from January 1, 2009 - December 31, 2013 were obtained from respective government agencies. Two types of datasets were used in the analysis; observed meteorological factors (MF) and its corresponding delayed or lagged effect (LG). After which, these datasets were subjected to the four modeling techniques. The predictive accuracy and variable importance of each modeling technique were calculated and evaluated. Results: Among the statistical modeling techniques, Random Forest showed the best predictive accuracy. Moreover, the delayed or lag effects of the meteorological variables was shown to be the best dataset to use for such purpose. Thus, the model of Random Forest with delayed meteorological effects (RF-LG) was deemed the best among all assessed models. Relative humidity was shown to be the top-most important meteorological factor in the best model. Conclusion: The study exhibited that there are indeed different predictive outcomes generated from each statistical modeling technique and it further revealed that the Random forest model with delayed meteorological effects to be the best in predicting the temporal pattern of Dengue incidence in Metropolitan Manila. It is also noteworthy that the study also identified relative humidity as an important meteorological factor along with rainfall and temperature that can influence this temporal pattern.

DOI： 10.1186/s12879-018-3066-0

Scopus

PubMed

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

© 2017, Society of Wetland Scientists. Constructed wetlands (CWs) for wastewater treatment had drawn much attention for their additional function of conserving freshwater ecosystems. The purpose of this study was to evaluate the macroinvertebrate biodiversity of two subsurface-flow and surface-flow hybrid CW systems (Qimei CW system (QCWS) and Kejiyuan CW system (KCWS)) under high and low pollutant stress in China. Nonmetric multidimensional scaling (NMDS) and canonical correspondence analysis (CCA) were used to analyze the water quality and aquatic macroinvertebrates community data. The results indicated that the subsurface-flow CW could conserve significant macroinvertebrate biodiversity (total taxon richness 17). The community structure was influenced by habitat types (i.e., subsurface-flow vs. surface-flow CWs) under low pollutant stress, and this influence was somehow masked by influence of water quality under high pollutant stress. Good pollutant removal rates were obtained in decreasing the concentrations of total nitrogen (66.37% and 27.28%), total phosphorus (58.93% and 37.55%), and loadings of biochemical oxygen demand (28.38% and 73.71%) in QCWS and KCWS, respectively. This study implied that subsurface-flow CWs could achieved good performance in both water purification and biodiversity conservation, which could provide a more solid basis for the application of subsurface-flow CWs.

DOI： 10.1007/s13157-017-0976-3

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acs.est.8b00590

Scopus

PubMed

researchmap

Authorship：Last author   Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;In order to examine that eDNA abundance could predict abundance of source freshwater insects, we performed meta-barcording analysis of freshwater insects using water environmental DNA (eDNA) in Shigenobu River, Ehime, Japan by Next generation sequencing (NGS) analysis. The eDNA, which is released from various organisms, is one of the most stractiong way to reveal and monitor species diverstity of aquatic animals. Although NGS analysis was employed meta-barcoding analysis for eDNA, a few studies reported the relationship between abundance of freshwater insects and regerated reads generated from NGS. At first, we collected 2L of river water from 12 sites and freshwater insects from 11 sites, 3 times. The water samples were filterd by 0.2 um membrane filter. DNA extraction was performed by Phenol-Chloroform method and ethanol presipitation. Extracted DNAs were purified by DNeasy Blood and Tissue kit (Qiagen) again. Chytochrome Oxidase I reagion was amplified using universal primer for Insects. NGS analysis was performed by MiSeq using v3 reagents (illumina). After quality control and reads screaning using primer sequence, operational taxonomic units (OTU) were clustered. The source animals of OTUs were searched in NCBI data base by BLAST. In result, we detected almost al families, which collected from field, from eDNA. The abudance of freshwater insects was compared with reads abundance. In result, we detected a lot of non-target species (e.g. microorganisms, crastaceans, inland insects). When high number of insect reds, PCR procedure should be improved. The number of freshwater insects showed positive relationship to read abundance from eDNA. It supported that eDNA analysis using NGS predict the abundance of freshwater insects.

DOI： 10.2208/jscejer.73.III_139

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

Proteome variation among natural populations along an environmental gradient may provide insights into how the biological functions of species are related to their local adaptation. We investigated protein expression in five stream stonefly species from four geographic regions along a latitudinal gradient in Japan with varying climatic conditions. The extracted proteins were separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization of time-of-flight (MALDI TOF/TOF), yielding 446 proteins. Low interspecies variation in the proteome profiles was observed among five species within geographical regions, presumably due to the co-occurring species sharing the environments. However, large spatial variations in protein expression were found among four geographic regions, suggesting strong regulation of protein expression in heterogeneous environments, where the spatial variations were positively correlated with water temperature. We identified 21 unique proteins expressed specifically in a geographical region and six common proteins expressed throughout all regions. In warmer regions, metabolic proteins were upregulated, whereas proteins related to cold stress, the photoperiod, and mating were downregulated. Oxygen-related and energy-production proteins were upregulated in colder regions with higher altitudes. Thus, our proteomic approach is useful for identifying and understanding important biological functions related to local adaptations by populations of stoneflies.

DOI： 10.1002/arch.21422

Scopus

PubMed

researchmap

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

A fundamental question linking population genetics and community ecology is how adaptive processes (e.g., natural selection) and neutral processes (e.g., drift-migration equilibrium) underpin the species-genetic diversity correlation (SGDC). Here, we combine genome scans and outlier loci detection with community analysis to separately test for neutral and nonneutral SGDCs in four species of stream insect. We sampled 60 localities in Japan and examined the relationships among population AFLP band richness (Br), taxon richness of the total community (S) and of the trophic guild (Str ), and 15 habitat parameters that could potentially drive adaptation and influence richness. Neutral Br was positively correlated with S only in the dominant species of these communities, suggesting Br may be constrained when intraspecific competition is pronounced. Nonneutral Br was correlated with Str in a species restricted to high elevations where habitat heterogeneity was highest. Community distance and genetic distance (β-SGDC) was correlated in two of the four species at both neutral and nonneutral loci. Distance-based redundancy analysis found geographic isolation and elevation to drive divergence of both communities and populations. This suggests that both neutral and adaptive divergence occurred through the shared influences of geographic isolation and local adaptation at the two levels of diversity.

DOI： 10.1111/evo.13261

Web of Science

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MAGNOLIA PRESS  

© 2017 Magnolia Press. The species of Leuctridae (Plecoptera) from Guangxi, China are reviewed. Two species of Rhopalopsole are described as new to science, R. cestroidea sp. nov. and R. triangulis sp. nov., belonging to the R. vietnamica group and the R. magnicerca group, respectively. Six species of Leuctridae are now known for Guangxi including two additional species of Rhopalopsole and one Paraleuctra recorded for the first time. The previously unknown female of R. duyuzhoui Sivec &Harper, 2008 is described from specimens from Guangxi. Characters are illustrated for the separation of R. furcospina (Wu, 1973) and R. sinensis Yang &Yang, 1993. Mitochondrial sequences of five Rhopalopsole species support their specific recognition.

DOI： 10.11646/zootaxa.4243.1.8

Web of Science

Scopus

PubMed

researchmap

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

Species distribution models were developed to predict the spatial patterns of the species diversity and the genetic diversity of stream organisms using a distributed hydrological model. We derived annual metrics of water depth and flow velocity in rivers using a hydrological model covering an entire catchment in northeastern Japan. We acquired geospatial data throughout the catchment and the presence records of six taxa within the part of the catchment. Subsequently we derived habitat suitability indices (HSIs) for these taxa using a frequency analysis or the maximum-entropy approach (MaxEnt) to predict three metrics of species diversity. The Shannon-Wiener's diversity index based on MaxEnt (Shannon_MaxE) most effectively represented empirical taxon richness. Subsequently, by comparing Shannon_MaxE and empirical genetic diversity for the four species of stream insects, we evaluated species-genetic diversity correlations (SGDCs). Of the four species, only one caddisfly species (Hydropsyche orientalis) displayed significant positive SGDCs. The result reflects the broad habitat range of this taxon throughout the catchment and its poor dispersal ability, whereas the other three species lacked SGDCs and displayed either a strong dispersal potential (Stenopsyche marmorata and Ephemera japonica) or a narrower habitat range within upstream domains (Hydropsyche albicephala). Finally, we estimated the spatial distribution of genetic diversity of H. orientalis populations based on the calculated Shannon_MaxE using the positive SGDC. This framework is promising for projecting future biodiversity in the context of anthropogenic perturbations such as climate change.

DOI： 10.1002/eco.1778

Web of Science

Scopus

J-GLOBAL

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society of Civil Engineers  

&nbsp;We investigated gene flow of 15 aquatic insects (3 of Ephemeroptera species; 12 of Diptera, Chironomidae species) in Shigenobu River (Ehime, Japan) using Next-Generation DNA Sequencing (NGS) system. We collected aquatic insects from 10 sites. Mixed DNAs from each site were extracted from mushed community sample. Cytochrome Oxidase subunit I region in mitochondrial DNA were amplified by COI primer with DNA tag indicating each sampling site and were genotyped using GS FLX system. Comparison of pairwise <i>F</i>sts between flagmented sited and between non-flagmented sited by intermittend interval showed that most spesies were not interrupted by intermittent interval other than <i>Baetis</i> sp. MK-2015d and <i>Chironomus kiiensis</i>. Although Chironomidae was predicted separated genetic structure and low gene flow because of their wing and body size, average pairwise <i>F</i>st supported stronger dispersal than Ephemeroptera. Chironomidae might dispersal by wind passively and could use habitat outside of river such as rice field. The limitations of this study were power of DNA reading by NGS and DNA barcording to identify species name. Because several dominant species were not detected, ideal sample volume must be clarified. In addition, The current DNA database have less information about Japanese aquatic insects. Study species could be expanding by DNA information in the database increased.

DOI： 10.2208/jscejer.72.III_489

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society of Civil Engineers  

&nbsp;We investigated the potential effect of sediment bypass tunnel that mitigate the ecological impact of dam reservoir on fragmented stream macroinvertebrate communities in Southeastern Swiss Alps. Using metabarcoding approach, we compared Bray-Curtis community's dissimilarities within rivers among 3 types of rivers: rivers fragmented by dams, rivers connected by sediment dam-bypass tunnel, and reference rivers (i.e. no dams).Among the three bypassed dams investigated, two showed significantly lower dissimilarities across the bypassed dams than non-bypassed dams, supporting the ecological mitigation effect of sediment bypass tunnel. However, one bypassed dam did not show a mitigation effect. This bypass has been operating only for 2 years, a period much shorter than the other two (=39 and 93 years), hence the elapsed time of bypass operation may not be enough to produce a mitigation effect. Overall, our metabarcoding approach allowed the rapid assessment of community structure of huge samples (i.e. 7369 individuals) collected from abroad for our research. Several technical limitations in the use of metabarcoding such as unsuccessful detection of rare taxa were also presented in this study.

DOI： 10.2208/jscejer.72.III_559

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SCIENCE PRESS  

Diarrheal diseases can be transmitted via both primary infection due to exposures to contaminated materials from the environment and secondary infection due to person-to-person contacts. Usually, the importance of secondary infection is empirically assessed by fitting mathematical models to the epidemic curves. However, these empirical models may not be applicable to other epidemic cases because they are developed only for the target epidemics and they don't consider the detail routes of infection. In our previous study, we developed a theoretical model taking into account the various routes of infection that commonly occur in households (e.g., shaking hands, food handling, and changing diapers). This model was made flexible and applicable to any epidemics by means of adjusting model parameters. In this study, we proposed a new index "Vulnerability indicator to secondary infection (VISI)", which expressed a ratio of secondary infection to primary infection risks and calculated this index in a simulated norovirus (NoV) epidemic that involved 10,000 households. The results demonstrated that households composed of more than three members including infant(s) had much higher levels of VISI (5-45) than two-member-households with VISI (0.1-4). These results concluded that the infants were likely to be a hub of secondary infections in highly dense families and therefore careful handling of diapers was deemed indispensible in such families to effectively control the secondary infections.

DOI： 10.1016/j.jes.2016.05.041

Web of Science

Scopus

PubMed

J-GLOBAL

researchmap

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Malaria Research Center  

Background &amp
objectives: Aedes aegypti (L.) is an efficient vector for arboviral diseases such as dengue. The wings of Ae. aegypti has been extensively studied in order to investigate population heterogeneity and structure by utilizing a landmark based geometric morphometrics (GMs) approach. The aim of this study was to examine and assess the wing geometry of Ae. aegypti in Metropolitan Manila. Methods: In total, 312 Ae. aegypti mosquitoes were collected from 98 sampling points using a mosquito light-trap from May 2014 to January 2015. A complete coverage of the wing was achieved by removing wing scales with chemical and physical treatment, leading to identification of 26 landmarks. Geometric morphometric analyses were employed and the spatial distance pattern was estimated using isolation by distance (IBD) and spatial autocorrelation (SA). Results: The results of the GM analyses revealed population heterogeneity and structuring in Ae. aegypti populations for both sexes using principal component and canonical variate analyses respectively. Moreover, IBD and SA only detected significant spatial structure in male Ae. aegypti populations while female population structures were homogeneous throughout the geographical area. Interpretation &amp
conclusion: The newly modified wing preparation procedure was able to capture a complete coverage of the wings of Ae. aegypti, thus providing a stronger separation power for very close populations in an urban area. It is also noteworthy that the results of IBD and SA supported the findings of GM in the population structuring of male and female Ae. aegypti. The outcome of the study increases our understanding of the vector, which would be useful in developing effective control strategies.

Scopus

PubMed

researchmap

Language：Japanese   Publisher：(一社)日本衛生学会  

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Water quality maintenance should be considered from an ecological perspective since water is a substrate ingredient in the biogeochemical cycle and is closely linked with ecosystem functioning and services. Addressing the status of live organisms in aquatic ecosystems is a critical issue for appropriate prediction and water quality management. Recently, genetic changes in biological organisms have garnered more attention due to their in-depth expression of environmental stress on aquatic ecosystems in an integrative manner. We demonstrate that genetic diversity would adaptively respond to environmental constraints in this study. We applied a self-organizing map (SOM) to characterize complex Amplified Fragment Length Polymorphisms (AFLP) of aquatic insects in six streams in Japan with natural and anthropogenic variability. After SOM training, the loci compositions of aquatic insects effectively responded to environmental selection pressure. To measure how important the role of loci compositions was in the population division, we altered the AFLP data by flipping the existence of given loci individual by individual. Subsequently we recognized the cluster change of the individuals with altered data using the trained SOM. Based on SOM recognition of these altered data, we determined the outlier loci (over 90th percentile) that showed drastic changes in their belonging clusters (D). Subsequently environmental responsiveness (E-k') was also calculated to address relationships with outliers in different species. Outlier loci were sensitive to slightly polluted conditions including Chl-a, NH4-N, NOX-N, PO4-P, and SS, and the food material, epilithon. Natural environmental factors such as altitude and sediment additionally showed relationships with outliers in somewhat lower levels. Poly-loci like responsiveness was detected in adapting to environmental constraints. SOM training followed by recognition shed light on developing algorithms de novo to characterize loci information without a priori knowledge of population genetics.

DOI： 10.3390/w8050188

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

We have determined nucleotide sequences of three genes encoding histone H3, 18S rRNA, and mitochondrial cytochrome oxidase subunit I (COI) from the mayfly community in Eastern Asia. All sequence data collected from 303 specimens comprising 80 taxa (63 identified to species, 14 to genus, and 3 to family) were deposited in GenBank/ EMBL/ DDBJ International Databases for further expansion of DNA barcoding projects of mayflies. Phylogenetic analysis based on the neighborjoining method among the three genes found that COI and H3 genes had relatively high evolutionary rates, and were not suitable in inferring phylogenetic relationships among families within the orders but were useful in identifying species (i.e., DNA barcoding). In contrast, the more conserved 18S rRNA gene was adequately informative for elucidating inter-family divergence, but not suitable for species identification.

DOI： 10.1515/dna-2016-0001

researchmap

Language：Japanese   Publisher：土木学会  

DOI： 10.2208/jscejhe.72.I_433

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

AimA central question in landscape genetics is how local adaptation among heterogeneous environments leads to genetic variation in natural populations. This article has two aims: (1) to examine associations between non-neutral genetic diversity of stream insect populations and environmental heterogeneity simulated by a hydrothermal model; and (2) to establish a novel framework for simulating the spatial distribution of adaptive genetic variation at catchment scale.
LocationNatori River basin, north-eastern Japan.
MethodsA distributed hydrothermal model was used to simulate current velocity, water depth and water temperature throughout the basin. We used empirical genetic data from 6-21 non-neutral amplified fragment length polymorphism (AFLP) loci in four target stream insect species found at 45 sampling sites. We constructed multiple regression models using allele frequencies at the non-neutral loci as objective variables and the hydrothermal and geographical parameters as candidate explanatory variables.
ResultsOf the four species, one caddisfly species provided strong evidence of local adaptation to the annual maximum water temperature. Assuming a priori that the regression models were applicable throughout the study area, we visualized the geographical distributions of genetically similar groups - UPGMA (unweighted pair group method with arithmetic mean) clusters based on genetic distance - and local genetic diversity along the simulated hydrothermal gradients. The UPGMA clusters of two caddisfly species (Hydropsyche orientalis and Stenopsyche marmorata) showed clear spatial turnover along an elevational gradient. Local genetic diversities of these species showed lower genetic diversity in lowland areas.
Main conclusionsThe predicted spatial patterns along an elevational gradient could be explained by a hypothetical mechanism whereby lowland habitats only accommodate genotypes adapted to severe thermal conditions. Our approach of combining a hydrothermal simulation with genome-wide scans of non-neutral loci is promising for the identification of evolutionary associations of adaptive loci to the temporal regime of hydrothermal conditions, such as maxima and temporal variability.

DOI： 10.1111/jbi.12392

Web of Science

Scopus

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;We investigated gene flow of <i>Epeorus latifolium</i> in Shigenobu River (Ehime, Japan) fragmented by 3 types of barriers (i.e. one reservoir dam, 98 weir&check dam, intermittent flow). 112 individuals (13 - 15 individuals / population, average = 14) were collected from 8 populations and genotyped using Cytochrome Oxidase subunit I region in mitochondrial DNA. Among the 3 types of habitat barriers, the weir&check dams showed significant and the highest pairwise <i>F</i>st between fragmented populations. In addition, the number of weir&check dams was significantly and positively correlated to the level of pairwise <i>F</i>st (<i>R</i> = 0.81, p < 0.05), suggesting that gene flow of <i>E</i>. <i>latifolium</i> tends to be interrupted in sections with large number of weir&check dams (at least > 14). However, this genetic differentiation found in mountain area could be also explained by the potential process of local adaptation of the populations to the different environmental conditions associated to elevational variation between sites (e.g. water temperature, water flow velocity, UV, oxygen concentration). <i>E. latifolium</i> did not show significant genetic differentiation in the other barrier types of reservoir dam and intermittent flow. It suggests that <i>E. latifolium</i> adults fly over the small-size reservoir dam that we studied (< 0.5 km²) and have adapted life history strategy to migrate across the intermittent sections.

DOI： 10.2208/jscejer.71.III_115

researchmap

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

The 2011 Great East Japan Earthquake generated extreme tsunami waves that entered river mouths, causing strong and long-distance propagation along the water corridors. This study reports the first empirical survey of this rare and extreme ecological impact of tsunami propagation on river communities. We sampled macroinvertebrate communities at 15 river sites on the Sendai Plain in Northeast Japan along a gradient of tsunami disturbance intensity [i.e. altitude: range=1-29m above sea level (a.s.l.)] two and 16months after the earthquake. In comparison with data collected before the tsunami, we found evidence of significant reductions in taxon richness (-54% on average) and total abundance (-91%) after the tsunami in inundated river reaches (up to 25km from the river mouth). There were large spatial variations in local impacts, with altitudes of 7-9m a.s.l. separating heavily damaged inundated communities from intact non-inundated communities. The degraded communities exhibited variable recovery rates extending beyond 16months after the tsunami. Mobility trait analysis revealed a relatively rapid recovery of swimmers (e.g. Baetis), reflecting their high mobility and active drift behaviours in the recolonisation phase. We observed the rare phenomenon of upstream drift' of marine worms (Phyllodocidae) to freshwater rivers in three of the 10 inundated sites. These survived in fresh water for up to 14months, suggesting that some marine-derived immigrants can persist in fresh water for extended periods.

DOI： 10.1111/fwb.12359

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：UNIV CHICAGO PRESS  

Most population genetic studies in streams infer long-term patterns of gene flow by calculating fixation indices (e. g., F-ST) among sampled populations. In more-recent analytical methods, the need to assign individuals to populations a priori (clustering algorithms) is relaxed, and spatial autocorrelation analysis of allele frequencies (SA) is used to infer finer-scale and potentially short-term dispersal distances. We applied multiple methods to study the population genetic structure of the riverine caddisfly Stenopsyche marmorata (Trichoptera:Stenopsychidae) from 4 adjacent catchments in northeastern Japan. We genotyped larval individuals (N = 532) from 30 sites at 8 polymorphic microsatellite loci. Fixation indices suggested low levels of genetic differentiation among populations (global F-ST = 0.062, p &lt; 0.01), and significant isolation-by-distance (IBD) indicated populations were in drift-migration equilibrium. Bayesian clustering separated S. marmorata into distinct upland (&gt; 250 m asl) and lowland populations, with different F-ST values (upland F-ST = 0.048, p &lt; 0.01; lowland F-ST = 0.029, p &lt; 0.01) and significant IBD only among upland populations. Allele frequencies were significantly positively autocorrelated (Moran's I &gt; 0, p &lt; 0.05) at distances up to 18 km along streams and up to 12 km across terrestrial habitat. These values were similar to directly observed flight distance in a single generation for this species in the field. We conclude that the multiple-method approach revealed: 1) unexpected population subdivision between upland and lowland areas that may result from local adaptation, differences in phenology, and historical colonization by multiple lineages; and 2) fine-scale estimates of dispersal that match direct observations of flight and suggest gene flow is more pronounced along water

DOI： 10.1086/675076

Web of Science

Scopus

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society of Civil Engineers  

We evaluated the linkage between genetic diversity and habitat suitability index (HSI) of aquatic animals including freshwater fishes, amphibians and macroinvertebrates in the Natori River basin located at the middle of Miyagi prefecture. The HSI has been structured by variables calculated from a distributed hydro-thermal model and GIS based geographical variables. We found a strong positive correlation between genetic diversity and HSI in one caddisfly (Hydropsychidae), indicating that a prospective habitat (i.e., locality which has high HSI) might contribute to increase in genetic diversity. The genetic diversity of one caddisfly (Hydropsychidae) had significant positive correlations with the HSIs of predatory fishes and aquatic insects. This result suggests that exposures to predation pressure can enhance genetic diversity. We derived negative correlations between genetic diversity and the HSIs within niche competitors, suggesting inter-species selective pressure constrains intra-species genetic diversity.

DOI： 10.2208/jscejhe.70.I_1405

J-GLOBAL

researchmap

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

A central question linking ecology with evolutionary biology is how environmental heterogeneity can drive adaptive genetic divergence among populations. We examined adaptive divergence of four stream insects from six adjacent catchments in Japan by combining field measures of habitat and resource components with genome scans of non-neutral Amplified Fragment Length Polymorphism (AFLP) loci. Neutral genetic variation was used to measure gene flow and non-neutral genetic variation was used to test for adaptive divergence. We identified the environmental characteristics contributing to divergence by comparing genetic distances at non-neutral loci between sites with Euclidean distances for each of 15 environmental variables. Comparisons were made using partial Mantel tests to control for geographic distance. In all four species, we found strong evidence for non-neutral divergence along environmental gradients at between 6 and 21 loci per species. The relative contribution of these environmental variables to each species' ecological niche was quantified as the specialization index, S, based on ecological data. In each species, the variable most significantly correlated with genetic distance at non-neutral loci was the same variable along which each species was most narrowly distributed (i.e., highest S). These were gradients of elevation (two species), chlorophyll-a, and ammonia-nitrogen. This adaptive divergence occurred in the face of ongoing gene flow (Fst = 0.01-0.04), indicating that selection was strong enough to overcome homogenization at the landscape scale. Our results suggest that adaptive divergence is pronounced, occurs along different environmental gradients for different species, and may consistently occur along the narrowest components of species' niche.

DOI： 10.1371/journal.pone.0093055

Web of Science

Scopus

PubMed

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;To predict transmission pattern of Norovirus gastroenteritis at regional scale, we've constructed an infectious disease transmission model that considers secondary infection in households. In the model, a household-scale submodel that could simulate secondary infection within family members was independently and in parallel applied to all households in the modeled region. The result of simulation showed that the effect of the secondary infection on the spread of the Norovirus gastroenteritis was larger than the primary infection. In particular, households of more than four family members including infant(s) showed ten times higher infectious risk of the secondary infection. Based on comparisons with empirical epidemiological data in the modeled region, our approach demonstrated a high utility ofthe model in estimating the exposure time fromthe primary infection time.

DOI： 10.2208/jscejer.70.III_295

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

This study quantitatively evaluated Habitat Suitability Index (HSI) and species diversity of benthic invertebrates using a distributed hydro-thermal model and observed benthic samples from 45 localities in the Natori River basin, Northeast of Japan. We applied probability density function and histogram analysis to the Suitability Index (SI) model analysis. To evaluate basin-scaled habitat values of invertebrate families, we used the index Weight Usable Ratio (WUR). From the viewpoint of repeatability in the multiple modeling manners, Diptera had the highest WUR in basin. We estimated spatial distribution of Shannon-Weiner diversity index based on the HSIs to evaluate species diversity throughout the basin. Results in the both applications showed that regions found in the top 80% of the diversity index were allocated from the upper to the middle stream.

DOI： 10.2208/jscejhe.70.I_1297

J-GLOBAL

researchmap

Language：Japanese  

DOI： 10.2208/jscejer.69.III_489

J-GLOBAL

researchmap

DOI： 10.1186/1471-2148-13-132

PubMed

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;Dams often supply large amount of Fine Particulate Organic Matters (FPOM) orginated from dam reservoirs (called "dam-POM") to their downstream reaches. It potentially leads to a shift of trophic sources of stream faunal communities from the stream-origins (e.g., periphyton and terrestrial input) to the dam-origin organic matters. However, the lentic habitats at dam outlet reaches such as backwater and isolated pool may receive a lot of terrestrial and autochthnous organic matters, thus we need habitat-specific tests regarding the trophic dependency on dam-POM. The aim of this study is to qularify the spatial variation of trophic contribution of dam-POM between lotic and lentic habitats at dam downstream reaches. The proportion of contribution of dam-POM to Suspended FPOM and Benthic FPOM was quantified at each habitat using a mixing source model with carbon and nitrogen stable isotopic data. The results support the habitat specific patterns showing higher contribution of dam-POM to Suspended FPOM in lotic habitats than lentic habitats. It suggests the ecologically important role of lentic habitats in locally sustaining insitu trophic structures of stream ecosystem in the impacted reaches by dams.

DOI： 10.2208/jscejer.69.III_547

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;Environmental heterogeneity among habitats may be one of the factors causing adaptive genetic divergence in wild populations. We examined the effect of natural selection on genetic diversity of <i>Stenopsyche marmorata</i> population in middle of Miyagi, Japan. Eight microsatellites were employed to draw genetic structure and tested for their neutrality. Three microsatellites were estimated to be presumably under the selection and 4 microsatellites were nuetrall. Genetic diversity at selective loci showed significant positive partial correlations with food condition (SS, FPOM, chlorophyll a within surface biofilm, FBOM) and riverbed heterogeneity (average size of pebbles, uniform coefficient of sediment). It support that linked geanes with lower fitness could exist under habitats with abundand foods and variable sediments. This results suggest a risk of decrease of genetic diversity by artificial environment disruption.

DOI： 10.2208/jscejer.69.III_489

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

We evaluated spatial variations of habitat suitability of nine aquatic insects in the Natori River basin, Northeast Japan quantitatively combining dual approaches of using Habitat Suitability Index (HSI) model analysis and hydrothermal simulation model. We also developed a new index of the Weighted Usable Ratio (WUR) for evaluating the overall habitat suitability throughout the catchment for each species. The results of spatial distributions of HSI showed species-specific patterns such as wide distribution of digit grids with high HSI throughout the catchment in habitat generalists (e.g., <i>Baetis</i>, WUR=0.55) while the constrained distribution in habitat specialists (e.g., <i>Limnocentropus insolitus</i>, WUR=0.06). Overall, the spatial variation of HSI showed good consistency with the empirical habitat distributions, supporting the accuracy of our model approach.

DOI： 10.2208/jscejhe.69.I_1255

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

In accordance with a most recent report in discipline &quot;landscape genetics&quot;, simulation studies to predict spatial genetic variation are urgently required. We evaluated relationships between genetic diversities of caddisfly Hydropsyche orientalis and species diversity calculated from Habitat Suitability Index (HSI) of aquatic animals and discussed outliers in the correlation diagrams in the Natori River basin located at the middle of Miyagi prefecture. Omitting two significant outliers, we developed spatial genetic diversity distribution maps on the basis of linear regression models in the correlation diagrams. At the catchment scale, the longitudinal distribution of genetic diversity had high peak in the middle stream. The result is identical with the observed pattern of genetic diversity for the species. The prediction has advantages which can be applied to future planning in respect of dam removal, urban planning, and zoning in view of genetic diversity.

DOI： 10.2208/jscejhe.69.I_1303

researchmap

Language：Japanese  

DOI： 10.2208/jscejer.68.III_611

J-GLOBAL

researchmap

Language：Japanese  

DOI： 10.3825/ece.15.121

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;Relationships between species diversity calculated from Habitat Suitability Index (HSI) of aquatic animalms and genetic diversities of four species of aquatic insect (three caddisflies and one mayfly) were examined in the Natori River basin, middle of Miyagi prefecture Japan. The HSI has been evaluated for six riverine animals based on current velocity and water depth calculated from an existing distributed runoff model and geographical data (eg., landuse, slope, and urban ratio) by Kazama et al. (2007). As a result, three out of four genetic diversity indices of one caddisfly (<i>Hydropsyche orientalis</i>) showed significant positive correlation to the HSI based taxa richness. This result suggests a sharing influence of immigration on community and population levels is potentially high in the locations where many species can coinhabit. Accordingly, parallel effects of gene flow and habitat heterogeneity on species and genetic diversity through environmental filtering and local adaptation of genotypes of focal species would contribute to the enhancement of biodiversity.

DOI： 10.2208/jscejer.68.III_603

J-GLOBAL

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Ecology and Civil Engineering Society  

Spatial structures of 7 types of aquatic habitat at the reach scale were delineated using a high precision Global Position System (GPS) in 3 dam-impacted and 6 reference riverrine reaches in Miyagi Prefecture, Japan. Taxonomic and functional compositions of quantitative benthic invertebrate samples were also investigated. Out of the three dams, two indicated decreased levels of habitat diversity indexes (S, H', a, and E) at the outlet reaches due to reduced sediment supply and reduced hydraulic variation through dam-operation
but one dam connected to meandering outlet reach did not. Percentage of total area of lentic habitats (i. e., backwater and isolated pool) at the dam-impacted reaches (mean= 1.5%) was significantly lower than reference reaches (mean=5.5%). The taxon richness, abundance of swimmers, predators, attachers and crawlers increased with the percentage of lentic habitat area (P&lt
0.05, n=9), which suggests that lentic habitats contribute to maintain species diversity and the functional groups at the reach scale. Our data indicated a high application potential of the GPS-based evaluation of habitat structure for biodiversity conservation in rivers.

DOI： 10.3825/ece.15.121

Scopus

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

&nbsp;Spatial environmental heterogeneity may favorably sustain genetic diversity of wild populations through adaptive divergence. We empirically examined the relationship between habitat structure and genetic diversity of stream mayfly <i>Ephemera strigata</i> populations at riverine reach scale. The habitat structure was delineated by a high precision GPS among 11 riverine sites in the Natori River, Miyagi Japan and genetic diversity was quantified at nine loci presumably under selection identified through a genome wide scan of 319 AFLP loci. Out of the nine adaptive loci, two had a strong association with the index of habitat structure, indicating a significant variation of allele frequency and genetic diversity along a gradient of the percentage of the total area of lentic habitats (i.e., backwater and isolated pool, range = 0.0 - 11.2 %). Particular environmental features reserved in these lentic habitats such as low current and high amount of deposited organic matters may enhance local adaptation of specific genotypes, leading to higher adaptive genetic variation within reaches. Our result highly suggests the importance of conservation of lentic habitats in river to sustaining genetic diversity of aquatic organisms.

DOI： 10.2208/jscejer.68.III_611

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)  

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WITPress  

In order to assess the impacts of sabo dam (check dam) construction, species diversity of benthic faunal communities and genetic diversity of caddisfly Stenopsyche marmorata were investigated using microsatellite analysis along the Oisawa River, Japan. The Oisawa River is fragmented by two slit sabo dams and one unslit sabo dam. Over the slit dams, Shannon-Weiner diversity indices and number of taxa ranged from 0.90 to 1.02 and 13 to 15 respectively in the upstream region, and also from 0.98 to 1.18 and 13 to 20 respectively in the downstream region. Through microsatellite analysis of S. marmorata, we found two selective alleles, which show clear genetic differentiation among three upstream sites and four downstream sites in the study area. Longitudinal change of mean heterozigosities Ho showed a decreasing pattern of genetic diversity in the downstream site (=0.486 - 0.678), and it showed the highest diversity at the highest upstream site, which receives no effect of the unslit dam. Differences of community's species compositions between sites were correlated to geographical distances whereas genetic distances between sites were not correlated to geographical distances, suggesting that species compositions of the communities were determined through the process of species adaptation to the local environment. We concluded that increments of species diversity at the inlet reaches of the slit dam were caused by the recovery of habitats, and by the continuousness of the stream which allows the flowing of particulate organic matters and sediments to the downstream area. In part of some loci, genetic selection might occur by the different velocities caused by sabo dam constructions, because S marmorata (filter-feeder) has the habit of constructing nets to filter and collect fine particulate organic matter (FPOM) from water flow. © 2011 WIT Press.

DOI： 10.2495/RM110231

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：JOHN WILEY & SONS LTD  

1. The dispersal ability of species and the geographic scale of habitat fragmentation both may influence the extent of gene flow between fragments, but their interactions have rarely been tested, particularly among co-occurring species.
2. Population genetic structures of three species of aquatic insect were compared in streams fragmented by reservoirs and in unfragmented streams in north-eastern Japan, using 52, 37, and 58 RAPD markers. The three species studied included a strong disperser Cincticostella elongatula (Ephemeroptera: Ephemerellidae), an intermediate disperser Stenopsyche marmorata (Trichoptera: Stenopsychidae), and a weak disperser Hydropsyche orientalis (Trichoptera: Hydropsychidae).
3. The patterns of genetic isolation by distance (IBD) supported a priori hypotheses of dispersal ability. The strong disperser (C. elongatula) exhibited significant IBD only at the largest spatial scale studied (among drainages, r = 0.50, P &lt; 0.01). The intermediate disperser (S. marmorata) showed IBD both within (r = 0.22, P &lt; 0.01) and among (r = 0.45, P &lt; 0.01) drainages. The weak disperser (H. orientalis) did not exhibit significant IBD at any scale.
4. Pairwise genetic differentiation (theta) indicated that neither the weak disperser nor the strong disperser were genetically differentiated above and below reservoirs when compared with reference sites. This was in contrast to previous results for S. marmorata, for which subpopulations were genetically fragmented across larger (&gt;4.1 km), but not smaller (&lt;2.9 km) reservoirs.
5. We suggest that intermediate dispersers, i.e. those at equilibrium between migration and genetic drift within drainages, are more likely to be affected by fragmentation than either strong or weak dispersers. Intermediate dispersers could therefore be used as indicator species in studies aimed at detecting the effects of distance between habitat fragments (e. g. reservoir size) for conservation planning. Copyright (C) 2010 John Wiley & Sons, Ltd.

DOI： 10.1002/aqc.1124

Web of Science

Scopus

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospora zeae-maydis; Chorthippus parallelus; Conyza canadensis; Cotesia sesamiae; Epinephelus acanthistius; Ficedula hypoleuca; Grindelia hirsutula; Guadua angustifolia; Leucadendron rubrum; Maritrema novaezealandensis; Meretrix meretrix; Nilaparvata lugens; Oxyeleotris marmoratus; Phoxinus neogaeus; Pristomyrmex punctatus; Pseudobagrus brevicorpus; Seiridium cardinale; Stenopsyche marmorata; Tetranychus evansi and Xerus inauris. These loci were cross-tested on the following species: Agalinis decemloba; Agalinis tenella; Agalinis obtusifolia; Agalinis setacea; Agalinis skinneriana; Cercospora zeina; Cercospora kikuchii; Cercospora sorghi; Mycosphaerella graminicola; Setosphaeria turcica; Magnaporthe oryzae; Cotesia flavipes; Cotesia marginiventris; Grindelia Xpaludosa; Grindelia chiloensis; Grindelia fastigiata; Grindelia lanceolata; Grindelia squarrosa; Leucadendron coniferum; Leucadendron salicifolium; Leucadendron tinctum; Leucadendron meridianum; Laodelphax striatellus; Sogatella furcifera; Phoxinus eos; Phoxinus rigidus; Phoxinus brevispinosus; Phoxinus bicolor; Tetranychus urticae; Tetranychus turkestani; Tetranychus ludeni; Tetranychus neocaledonicus; Tetranychus amicus; Amphitetranychus viennensis; Eotetranychus rubiphilus; Eotetranychus tiliarium; Oligonychus perseae; Panonychus citri; Bryobia rubrioculus; Schizonobia bundi; Petrobia harti; Xerus princeps; Spermophilus tridecemlineatus and Sciurus carolinensis.

DOI： 10.1111/j.1755-0998.2009.02746.x

Web of Science

researchmap

Language：Japanese   Publisher：Japan Society on Water Environment  

Population connectivity of aquatic organisms is an important factor for sustaining the robustness of metapopulation in a basin-scale as well as for interpreting the properties of respective local populations. We examined the genetic structure of 30 <i>Stenopsyche marmorata</i> (Tricoptera) local populations in 4 catchments in South-Central Miyagi Prefecture, Japan using AFLP markers, and evaluated their dispersal pattern. We constructed a dendrogram using q, and found that 7 populations in headwater were isolated from another area in the basins. Although previous studies on S. marmorata adult flight within streams reported most of the females fly upstream along a watercourse after mating, our results indicated high possibility of non-directional adult dispersal due to wind. The results also suggested a strong potential for DNA polymorphic analysis to enhance understanding of the population connectivity and dispersal patterns of aquatic organisms in a basin scale.

DOI： 10.2965/jswe.32.253

CiNii Books

J-GLOBAL

researchmap

Other Link： http://hdl.handle.net/2433/148410

Language：English   Publishing type：Part of collection (book)   Publisher：World Scientific Publishing Co.  

On the upstream of Atago weir, Hirose River, Japan, the sedimentation phenomenon is complex due to the coexistence of the weir and channel bend. Moreover, fine sediment accumulates on the river bed notably. From the observed results, it is found that silt is the major component in the flood water and accumulates significantly in the discharge increasing stage by the backwater of the weir. From the theoretical computation considering the settling and resuspension of the suspended sediment, the cohesion of fine sediment is an important factor of the net deposition process.

DOI： 10.1142/7073-vol11

Scopus

researchmap

Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：TSINGHUA UNIVERSITY PRESS  

On the upstream of Atago weir, Hirose River, Japan, the sedimentation phenomenon is complex due to the coexistence of the weir and channel bend. Moreover, fine sediment accumulates on the river bed notably not only the floodplain. In this study, we conducted field observation and numerical computation. From the observation results, we found that fine sediment is the major component in the flood water and accumulates significantly close to the inner bank (silt and clay= 55% in the maximum). In the numerical computation, we calculated the flow using finite volume method with unstructured grid. The suspended-sediment transport was calculated by the advection-diffusion equation considering settling and resuspension. By considering the weir and cohesivity of the fine sediment, we reproduced the settling-resuspension characteristics of the fine sediment. From this calculation result, the deposition and erosion region was indicated.

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER BASEL AG  

Small local populations may have low levels of genetic diversity, although widespread gene flow may counteract genetic drift and maintain high local diversity. At a larger spatial scale, a relationship between population size and genetic diversity could have important effects on the longitudinal genetic patterns in riverine corridors. We examined the genetic structure of 15 subpopulations of the caddisfly Hydropsyche orientalis in four rivers within the Natori River system in Japan using 78 polymorphic RAPD loci. Six subpopulations were fragmented by reservoirs and thus may be smaller, genetically isolated, or both. Pairwise F (ST) values were generally high throughout the study, and a higher mean value across the reservoirs (mean=0.219) relative to unfragmented streams 0.157) indicated reduced gene flow. Genetic diversity was not consequently reduced in these subpopulations; however, genetic diversity was positively correlated with larval density at all sites (percentage of polymorphic loci, %P = 46.3-59.1; expected heterozygosity H (exp) = 0.163-0.225). The longitudinal distribution of genetic diversity exhibited a unimodal pattern, with a peak in middle reaches. Correlation with larval density was a significant factor, although the presence of cryptic species as well as unimodally distributed environmental variables (e.g., habitat heterogeneity) provide alternative hypotheses. We suggest that studies examining longitudinal patterns of genetic diversity will provide a better understanding of the principal mechanisms that create and maintain biodiversity in rivers and streams.

DOI： 10.1007/s00027-008-8099-9

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

Analysis of carbon (delta C-13) and nitrogen (delta N-15) stable isotopes provides an increasingly important means of understanding the complex trophic structure of macroinvertebrate communities in streams. We coupled a stable isotope approach with a contaminant analysis of six metals (Pb, Ag, Zn, Hg, Cu, As) to trace the accumulation and dilution of metals from an abandoned mine across trophic levels of the benthic community in Ginzan Creek, Japan. The delta N-15 signature increased with trophic level, with mean increases of 4.70%. from producers to primary consumers and 3.06%. from primary to secondary consumers. Tissue Pb and Ag concentrations were negatively correlated with delta N-15, indicating biodilution of both metals through the food web. Although macroinvertebrate taxon body mass was negatively correlated with tissue metal concentration at several sites, it did not increase with trophic level (as delta N-15) in any of the sites, suggesting that changes in body mass were not the cause of biodilution. Our findings suggest invertebrates at higher trophic levels may exhibit increasingly efficient excretion of metals. Autotrophic epilithon (mean delta C-13 =-21.3%.) had a much higher concentration of mined metals than did riparian vegetation (mean delta C-13 = -29.3%.); nonetheless, a carbon-mixing model indicated that taxa. feeding on autochthonous carbon sources did not accumulate more metal than allochthonous feeders. It is likely that the notably high metal concentration of allochthonous FPOM plays an important role in the trophic transfer of metals. Our data suggest the strong potential for stable isotope analysis to enhance our understanding of metal transfer through stream macroinvertebrate food webs. (c) 2008 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.scitotenv.2008.01.006

Web of Science

Scopus

PubMed

researchmap

Language：Japanese  

DOI： 10.2208/prohe.52.1159

J-GLOBAL

researchmap

Language：Japanese  

DOI： 10.2208/prohe.52.1171

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society on Water Environment  

The genetic diversity of <i>Stenopsyche marmorata</i> was investigated in three spatial hierarchies, among catchments, among reaches within catchments, and among individuals within reaches, by AFLP analysis of 306 individuals in 12 reaches in 3 catchments in Eastern Japan. The analysis of molecular variance revealed that the diversity within reaches accounted for the highest percentage (86%) of the total genetic variation in the three hierarchies. The genetic diversity between reaches increased with geographical distance between reaches on a small spatial scale (=2-74km); however, it did not increase on a large spatial scale (=74-450km). The level of gene flow among adjacent catchments (=55-74km) was lower than that between reaches within catchments (=2-19km). The genetic diversity within reaches (as the percentage of polymorphic loci and expected heterozygosity) positively correlated with altitude, suggesting that altitude is an environmental factor in determining the local population size of <i>S. marmorata</i> in each reach.

DOI： 10.2965/jswe.31.31

CiNii Books

J-GLOBAL

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：I W A PUBLISHING  

The trophic structure of a macro invertebrate community was investigated using carbon and nitrogen stable isotopes analysis (delta C-13 and delta N-15) at three longitudinal sites along the main channel of the Hirose River, Japan, and at an outlet site of the tributary reservoir. Along the main channel, primal food supply gradually changed from allochthonous to autochthonous source. The percentage of autochthonous origin of the macroinvertebrate community calculated by a carbon-mixing model ( = 38-83%) increased with that of suspended fine particulate organic matter (FPOM = 21-67%), a main resource for filtering-collectors that dominated the communities. The lowest percentage of autochthonous in the lake outlet community ( = 21%) was a result of the notably low percentage autochthonous of FPOM due to the decomposition of allochthonous coarse particulate organic matter (e.g., leaf) strained in the reservoir. Predators had higher mean delta N-15 than non-predators in three sites. The average enrichments of delta N-15 signature per trophic level for all sites were 3.82 parts per thousand from producers to first consumers and 1.24 parts per thousand from first to second consumers. Our data suggest the strong potential for stable isotope analysis to enhance our understanding of the trophic structure of stream macroinvertebrate community.

DOI： 10.2166/wst.2008.679

Web of Science

Scopus

PubMed

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

We examined the genetic structure of 58<I>H</I>. orientalis populations in 6 catchments in South-Central Miyagi prefecture, Japan using AFLP markers. Based on the frequency distribution we observed in values of expected heterozygosity (H<SUB>e</SUB>), we formulated five categories of H<SUB>e</SUB>, e. g., <0.136=very low, 0.136<H<SUB>e</SUB><0.171=low, etc. We constructed a dendrogram of the 58 populations using θ, and classified the populations into 3 genetic groups (A, B, C) based on the genetic similarity (e. g., θ<0.25). Groups A and B were composed of 56 populations that could be divided into upstream (A) and downstream reaches (B) with a boundary occurring between 270m and 350m in elevation. Non-overlapping mating seasons that result from different timings of the life cycle was considered to be the cause of this difference in elevational genetic groups. Group C consisted of 2 isolated populations that are both located in narrow headwater streams that are isolated from downstream areas by lentic habitat downstream.

DOI： 10.11532/proes1992.44.83

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

We developed a model for stream aquatic insect populations to simulate geneti differentiation between fragments above and below a reservoir. The model includes parameters of population size, dispersal rate, mutation rate, and magnitude of habitat fragmentation caused by reservoir. According to the model, genetic distance (Nei's D<SUB>xy</SUB>) between the fragments increased following dam construction, and finally reached maximum differentiation within 10-50 generations. We simulated genetic differentiation of Stenopsyche marmorata (Trichoptera, Stenopsychidae) populations based on an observed data set of D<SUB>xy</SUB> at 6 dam sites, and evaluated the proportion of individuals unable to migrate across the reservoirs due to the dam constriction. The result indicated migration was largely reduced (i. e., 20-60% individual migrating) in 2 dams and was completely halted where D<SUB>xy</SUB> was highest.

DOI： 10.11532/proes1992.44.99

J-GLOBAL

researchmap

Language：Japanese  

DOI： 10.2208/jscejg.63.93

J-GLOBAL

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCI LTD  

Reservoirs have the potential to block the gene flow of stream macroinvertebrates along a Received 27 February 2006 channel, which takes place via larval drift and adult flight, resulting in genetic differentiation Received in revised form above and below the reservoirs. Gene flow across the reservoirs may be strongly obstructed if 15 November 2006 the area of standing water is larger. Using random amplified polymorphic DNA markers, we Available online 17 January 2007 investigated the genetic structure of Stenopsyche marmorata (Stenopsychidae: Trichoptera) populations found above and below the reservoirs, and the reference stream of six reservoirs Keywords: with small to large water surface area ranging from 0.12 km(2) to 6.00 km(2). As a result, the two largest reservoirs with a standing water area larger than 3.27 km(2) showed significant differ flow ences in pairwise 0 between fragmented and reference streams, whereas the other four res fragmentation ervoirs with a standing water area smaller than 1.64 km(2) showed insignificant differences. Genetic diversity The genetic differentiations in the two largest reservoirs did not result in the reduction of Population size genetic diversities in the fragments. Based on the significant correlation between relative population size and mean expected heterozygosity, we concluded that local genetic diversities were constrained in smaller populations due to the effect of genetic drift. (C) 2006 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biocon.2006.11.014

Web of Science

Scopus

researchmap

Language：Japanese  

DOI： 10.2208/jscejg.63.186

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

Based on RAPD, the genetic structures of <i>Stenopsyche marmorata, Hydropsyche orientalis</i>, and <i>Ephemerella nigra</i> populations found above and below reservoirs with various water surface areas were investigated. The results of the genetic distance showed that amongst the six reservoirs, the genetic differentiations of <i>H. orientalis</i> were found in the third smallest and in the largest reservoirs while those of <i>S. marmorata</i> were shown in the two largest reservoirs. The size of reservoir influenced the genetic differentiations for <i>S. marmorata</i>, but did not for <i>H. orientalis</i>. The expected heterosygosity indicated that genetic diversities of <i>H. orientalis</i> were constrained by both effects of habitat fragmentation and population size while the rest of the species were constrained only by the latter's effect.

DOI： 10.2208/jscejg.62.94

CiNii Books

researchmap

Language：Japanese  

DOI： 10.2208/jscejg.62.94

CiNii Books

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society on Water Environment  

Concentrations of Ag, Pb, and Hg in samples of aquatic insects and feeding sources such as algae and leaves were determined at four sites along the Ginzan River, Which receives drainage from a mined land. Nitrogen (&delta;¹⁵N) and carbon (&delta;¹³C) isotope analyses of the samples were carried out to assess whether feeding sources or the trophic level influenced metal accumulation in each faunal taxon. The individual dry mass was also measured to examine the effect of body size on metal accumulation. The relationship between metal concentrations and level of &delta;¹⁵N in fauna indicated biodilutions for Pb (3 sites) and Ag (1 site) through the food webs. This must be caused by high concentrations of metals in algae and fine particulate organic matter (FPOM) as the main feeding sources for harvivores and detorivores. The relative contribution of autochthonous and allochthonous energy sources to each taxon's biomass had no effect on the concentrations of Ag, Pb, and Hg in the fauna. The individual dry mass was negatively correlated with the concentrations of Ag (3 sites), Pb (1 site), and Hg (4 sites) in fauna.

DOI： 10.2965/jswe.28.737

CiNii Books

J-GLOBAL

researchmap

Other Link： http://hdl.handle.net/10097/52903

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

A macroinvertebrate community having been pre-assessed to have lower resilience against anthropogenic pulse-disturbance in river (e.g. muddy water from construction site) should be given intensive preservation efforts. Using stochastic population dynamics model with density-dependant and environmental fluctuation, a recovery prediction model for macroinvertebrates' total biomass following its decline due to pulse-stressors have been developed. In this recovery model, the inherent resilience was determined by three parameters: carrying capacity, specific growth rate and environmental fluctuation intensity. The parameters were estimated from the time-series data of the total biomass. Using the application of the recovery model for a hypothesized scenario, the recovery index (RI) and the ecological time loss (ETL) were calculated. Both RI and ETL can evaluate the recovery process of the total biomass after an anthropogenic pulse-disturbance.
A case study was done in 70 communities in Tokyo using the indexes of both RI and ETL. Recovery processes were simulated by administering a virtual pulse stressor to decrease the total biomass to 8%. As a result, 80% among the total number of communities performed a higher resilience within 3 years of the ETL. RI revealed that geographically neighboring community possesses extra-high resilience. Multiple regression analysis also proved that ETL was significantly explained by five environmental variables: specific conductivity, width of low-water channels, catchment area, suspended solid and stream order. As the results implied, macroinvertebrate community can recover quickly in total biomass when supplied from non-damaged upstream reaches. The case study also demonstrated the high potentialities of RI and the ETL for impact assessments in the river ecosystems. (c) 2005 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.ecolmodel.2005.04.014

Web of Science

Scopus

researchmap

Language：Japanese   Publisher：Japan Society on Water Environment  

The trophic structures of benthic faunal communities in a river are expected to respond to the relative amounts of autochthonous and allochthonous energy sources in the environment. Using isotopic analysis of &delta;¹³C, we investigated the percentage of autochthonous energy of benthic fauna at 3 sites longitudinally sited along the Hirose River, and at a site at the outlet of the Okura reservoir in a tributary. The relative trophic level of each taxon was also estimated from &delta;¹⁵N, and a biomass pyramid for each community was produced. As a result, the percentage of autochthonous energy of benthic faunal community formed a longitudinal distribution along the river, showing a high peak midstream with lower values both upperstream and downstream. This profile was similar to a longitudinal pattern of that of Fine Particulate Organic Matters (FPOM) in flowing water, a main resource for filtering-collectors that dominate these communities. At the Okura reservoir outlet, the percentage of autochthonous energy of the community was the lowest among the four sites, reflecting a low percentage of autochthonous energy in FROM due to the decomposition of allochthonous organic matter in standing water. The biomass pyramid for each community showed an energy peak in the middle trophic level.

DOI： 10.2965/jswe.28.385

CiNii Books

J-GLOBAL

researchmap

Other Link： http://hdl.handle.net/10097/52988

Language：Japanese   Publisher：Japan Society of Civil Engineers  

Reservoir obstructs gene flow of stream invertebrates along a channel, which takes place via larval drift and adult flight. It may result in genetic differentiation between above and below reservoir, which would reduce genetic diversities in fragments. Gene flow across a reservoir may be more strongly obstructed if the area of standing water is larger. Using RAPD, we investigated the genetic structure of <i>Stenopsyche marmorata</i> populations found above and below the reservoir, and the reference stream of 6 reservoirs in small to large water surface area. As a result, out of the 6 reservoirs, significant genetic differentiations were detected in the biggest 2 reservoirs with an area of standing water larger than 3.27 km². However, these genetic differentiations did not result in the reduction of genetic diversities in the fragments.

DOI： 10.2208/jscej.2005.790_49

CiNii Books

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

The population dynamics model describes the annual change of total biomass of benthic animals in river. We developed a recovery model assessing the influence of an artificial pulse type (temporal) impact with two evaluation indexes, i. e. Recovery Rate and Ecological Loss Time. Recovery simulations from a virtual impact were performed in 70 river sites in Tokyo with an assumption that the biomass decreases to 0.08 times just after the impact in each site. This recovery model confirmed the tendency of high resilience of macroinvertebrate as well as the effectiveness of the two indexes for impact assessments in river ecosystem.

DOI： 10.2208/jscej.2003.748_67

CiNii Books

J-GLOBAL

researchmap

Language：Japanese  

DOI： 10.2208/jscej.2003.734_99

CiNii Books

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society on Water Environment  

It has been reported that several floras and faunas in rivers are genetically impaired by artificial structures such as dams and cities because of the fragmentation and the reduced heterogeneity of their habitats. However, the investigations on genetic diversities of Trichoptera in rivers have been few to date. In this study, therefore, the genetic investigation of <i>Hydropsyche orientalis</i> was conducted at 13 stations in the Natori River System in Miyagi Prefecture, Japan from Dec. 2000 to May 2001. The Okura and Kamafusa dams, and the Sendai urban area are located upstream and downstream of the rivers. The DNA polymorphisms of <i>Hydropsyche orientalis</i> collected at the 13 stations were analyzed with the appropriate primer, OPA-11, for calculating the genetic diversity index. The result of statistical analysis of the index showed a significantly higher level of genetic diversity of <i>Hydropsyche orientalis</i> in the natural area than in the areas around the dams. Moreover, the effect of urbanization on its genetic diversity was indicated based on the estimation of its migration from the results of the cluster analysis of the DNA polymorphisms.

DOI： 10.2965/jswe.26.223

CiNii Books

J-GLOBAL

researchmap

Other Link： http://hdl.handle.net/10097/52936

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

CiNii Books

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：English   Publisher：UNIV MELBOURNE, MELBOURNE SCH ENGINEERING  

Among key components threatening global biodiversity in the future, such as land use alterations, climate changes can be primary stressors on stream ecosystems. Although many studies in the discipline of landscape genetics have tried to explore genetic consequences of oncoming climate change, most of them used anonymous genes, even though the majority of these genetic regions are just known to be "neutral" (no relation) to natural selection. In this study, we projected adaptive genetic variations and species distributions in four stream insect species under climate change scenarios, using a distributed hydrothermal model and 8 global climate models (GCM). We used non-neutral AFLP (Amplified Fragment Length Polymorphism) loci, which were statistically defined to be subject to natural selection, in the target species found at 11-31 out of 45 sampling localities. For modeling species distributions, we used presence data of the studied insects at the same sites as above. We acquired bias-corrected air temperature and precipitation data of 3 RCP (Representative Concentration Pathways) scenarios from the GCMs. Then, these climatic data were used to calculate annual metrics of current velocity, water depth and water temperature using the hydrothermal model. Models describing adaptive genetic variation, which is represented as linear response of allele frequency at each locus to environmental predictors, have been constructed based on current hydrothermal variables in a previous work. We used the model for estimating future ranges of the allele frequency along changing climate gradients. Finally, we projected spatial pattern of genetic diversity (heterozygosity) for the studied species. Also, we employed maximum entropy model (Maxent) to predict present and future distributions of the studied species. Degraded pattern of genetic diversity in a caddisfly species (Stenopsyche marmorata) was more notable under extreme climate scenarios when comparing with the current climate, depending on higher air temperature projected in the future. While genetic diversity in catchment scale only decreased in S. marmorata, habitat range, which was predicted by species distributions, severely contracted more in other three species than for S. marmorata.

Web of Science

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

We evaluated the linkage between genetic diversity and habitat suitability index (HSI) of aquatic animals including freshwater fishes, amphibians and macroinvertebrates in the Natori River basin located at the middle of Miyagi prefecture. The HSI has been structured by variables calculated from a distributed hydro-thermal model and GIS based geographical variables. We found a strong positive correlation between genetic diversity and HSI in one caddisfly (Hydropsychidae), indicating that a prospective habitat (i.e., locality which has high HSI) might contribute to increase in genetic diversity. The genetic diversity of one caddisfly (Hydropsychidae) had significant positive correlations with the HSIs of predatory fishes and aquatic insects. This result suggests that exposures to predation pressure can enhance genetic diversity. We derived negative correlations between genetic diversity and the HSIs within niche competitors, suggesting inter-species selective pressure constrains intra-species genetic diversity.

DOI： 10.2208/jscejhe.70.I_1405

researchmap

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

This study quantitatively evaluated Habitat Suitability Index (HSI) and species diversity of benthic invertebrates using a distributed hydro-thermal model and observed benthic samples from 45 localities in the Natori River basin, Northeast of Japan. We applied probability density function and histogram analysis to the Suitability Index (SI) model analysis. To evaluate basin-scaled habitat values of invertebrate families, we used the index Weight Usable Ratio (WUR). From the viewpoint of repeatability in the multiple modeling manners, Diptera had the highest WUR in basin. We estimated spatial distribution of Shannon-Weiner diversity index based on the HSIs to evaluate species diversity throughout the basin. Results in the both applications showed that regions found in the top 80% of the diversity index were allocated from the upper to the middle stream.

DOI： 10.2208/jscejhe.70.I_1297

researchmap

Language：Japanese  

CiNii Books

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：English   Publisher：TSINGHUA UNIV  

We evaluated spatial variations of habitat suitability of nine aquatic insects in the Natori River basin, Northeast Japan quantitatively combining dual approaches of using Habitat Suitability Index (HSI) model analysis and hydrothermal simulation model. We also developed a new index of the Weighted Usable Ratio (WUR) for evaluating the overall habitat suitability throughout the catchment for each species. The results of spatial distributions of HSI showed species-specific patterns such as wide distribution of digit grids with high HSI throughout the catchment in habitat generalists (for example, Baetis, WUR=0.55) while the constrained distribution in habitat specialists (for example, Limnocentropus insolitus, WUR=0.06). Overall, the spatial variation of HSI showed good consistency with the empirical habitat distributions, supporting the accuracy of our model approach.

Web of Science

researchmap

Language：Japanese   Publisher：Japan Society of Civil Engineers  

In accordance with a most recent report in discipline "landscape genetics", simulation studies to predict spatial genetic variation are urgently required. We evaluated relationships between genetic diversities of caddisfly Hydropsyche orientalis and species diversity calculated from Habitat Suitability Index (HSI) of aquatic animals and discussed outliers in the correlation diagrams in the Natori River basin located at the middle of Miyagi prefecture. Omitting two significant outliers, we developed spatial genetic diversity distribution maps on the basis of linear regression models in the correlation diagrams. At the catchment scale, the longitudinal distribution of genetic diversity had high peak in the middle stream. The result is identical with the observed pattern of genetic diversity for the species. The prediction has advantages which can be applied to future planning in respect of dam removal, urban planning, and zoning in view of genetic diversity.

DOI： 10.2208/jscejhe.69.I_1303

J-GLOBAL

researchmap

Language：Japanese   Publisher：土木学会  

DOI： 10.2208/jscejhe.69.I_1303

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：English  

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese   Publisher：Tohoku University  

CiNii Books

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese   Publisher：Tohoku University  

CiNii Books

J-GLOBAL

researchmap

Language：Japanese  

CiNii Books

J-GLOBAL

researchmap

Language：Japanese  

CiNii Books

J-GLOBAL

researchmap

researchmap

Language：Japanese  

researchmap

Language：English   Presentation type：Poster presentation  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

Language：Japanese  

researchmap

researchmap

researchmap

researchmap

researchmap