Updated on 2025/06/06

写真a

 
Takada Masatsugu
 
Organization
Graduate School of Agriculture Department of Science and Technology for Biological Resources and Environment Associate Professor
Title
Associate Professor
External link

Degree

  • 博士（エネルギー科学） ( 2017.3   京都大学 )

  • 修士（エネルギー科学） ( 2014.3   京都大学 )

  • 学士（農学） ( 2012.3   京都大学 )

Research Interests

  • Lignin

  • リグニン発光材料

  • Lignocellulose

  • Pretreatment

  • Wood pellet

  • Hydrothermal treatment

  • Topochemistry

  • Biomass

Research Areas

  • Life Science / Wood science

  • Nanotechnology/Materials / Energy chemistry

Research History

  • Ehime University   Faculty of Agriculture Department of Science and Technology for Biological Resources and Environment   Associate Professor

    2025.4

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  • Tokyo University of Agriculture and Technology   Graduate School of Bio-Applications and Systems Engineering   Assistant Professor

    2022.2 - 2025.3

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Professional Memberships

Papers

  • Effects of feruloyl-CoA 6′-hydroxylase 1 overexpression on lignin and cell wall characteristics in transgenic hybrid aspen Reviewed

    Naning Wang, Masatsugu Takada, Shingo Sakamoto, Ruben Vanholme, Geert Goeminne, Hoon Kim, Soichiro Nagano, Naoki Takata, Naofumi Kamimura, Mikiko Uesugi, Akiko Izumi-Nakagawa, Eiji Masai, Nobutaka Mitsuda, Wout Boerjan, John Ralph, Shinya Kajita

    Frontiers in Plant Science   16   2025.3

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    In plant cell walls, lignin, cellulose, and the hemicelluloses form intricate three-dimensional structures. Owing to its complexity, lignin often acts as a bottleneck for the efficient utilization of polysaccharide components as biochemicals and functional materials. A promising approach to mitigate and/or overcome lignin recalcitrance is the qualitative and quantitative modification of lignin by genetic engineering. Feruloyl-CoA 6′-hydroxylase (F6′H1) is a 2-oxoglutarate-dependent dioxygenase that catalyzes the conversion of feruloyl-CoA, one of the intermediates of the lignin biosynthetic pathway, into 6′-hydroxyferuloyl-CoA, the precursor of scopoletin (7-hydroxy-6-methoxycoumarin). In a previous study with Arabidopsis thaliana, we demonstrated that overexpression of F6′H1 under a xylem-preferential promoter led to scopoletin incorporation into the cell wall. This altered the chemical structure of lignin without affecting lignin content or saccharification efficiency. In the present study, the same F6′H1 construct was introduced into hybrid aspen (Populus tremula × tremuloides T89), a model woody plant, and its effects on plant morphology, lignin chemical structure, global gene expression, and phenolic metabolism were examined. The transgenic plants successfully overproduced scopoletin while exhibiting severe growth retardation, a phenotype not previously observed in Arabidopsis. Scopoletin accumulation was most pronounced in the secondary walls of tracheary elements and the compound middle lamella, with low levels in the fiber cell walls. Overexpression of F6′H1 also affected the metabolism of aromatics, including lignin precursors. Heteronuclear single-quantum coherence (HSQC) NMR spectroscopy revealed that scopoletin in cell walls was bound to lignin, leading to a reduction in lignin content and changes in its monomeric composition and molar mass distribution. Furthermore, the enzymatic saccharification efficiency of the transgenic cell walls was more than three times higher than that of the wild-type plants, even without pretreatment. Although addressing growth inhibition remains a priority, incorporating scopoletin into lignin demonstrates significant potential for improving woody biomass utilization.

    DOI： 10.3389/fpls.2025.1543168

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  • Synergetic effect of fungal pretreatment and lignin modification on delignification and saccharification: a case study of a natural lignin mutant in mulberry

    James Paul T. Madigal, Masami Terasaki, Masatsugu Takada, Shinya Kajita

    Biotechnology for Biofuels and Bioproducts   18 ( 1 )   2025.1

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1186/s13068-025-02611-y

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    Other Link： https://link.springer.com/article/10.1186/s13068-025-02611-y/fulltext.html

  • Lignin Distribution within the Lignocellulose Cell Wall and the Topochemistry of Delignification by Thermochemical Treatment

    Masatsugu Takada, Masami Terasaki

    Mokuzai Gakkaishi   70 ( 4 )   105 - 119   2024.10

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Japan Wood Society  

    DOI： 10.2488/jwrs.70.105

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  • Fractionation of beech wood cell walls into digestible cellulose-rich residues and photoluminescent lignin-rich precipitates <i>via</i> semi-flow hot-compressed water treatment with 2-naphthol

    Masatsugu Takada, Yutaka Okazaki, Shinya Kajita, Haruo Kawamoto, Takashi Sagawa

    RSC Advances   14 ( 29 )   20660 - 20667   2024

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    Hydrothermal treatment with 2-naphthol is an effective approach for the complete utilization of cell wall components from wood biomass.

    DOI： 10.1039/d4ra03094j

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Books

  • バイオマス (木材科学講座10)

    髙田 昌嗣, 宮藤久士（ Role： Contributor第1章3節 資源・エネルギー問題と木質バイオマス）

    海青社  2025.3  （ ISBN:4860990706 ）

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    Total pages：220  

    ASIN

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  • バイオマス (木材科学講座10)

    髙田 昌嗣（ Role： Contributor第1章4節 地球環境問題と木質バイオマス）

    海青社  2025.3  （ ISBN:4860990706 ）

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    Total pages：220  

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  • 月刊BIOINDUSTRY 2025年3月号

    髙田 昌嗣, 岡﨑 豊（ Role： Contributor乾式メカニカル処理による難溶解性リグニンの可溶化）

    2025.3 

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Presentations

  • Effect of fungal pretreatment on the delignification and saccharification of CAD-deficient mulberry wood

    James Paul T Madigal, Masatsugu Takada, Shinya Kajita

    ISWST2025  2025.3 

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  • Effects of incorporating scopoletin on lignin structure in transgenic hybrid aspen with the overproduction of feruloyl-CoA 6'-hydroxylase

    2024.3 

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  • Heterogenous molecular weight distribution of luminophores in MWLs from various lignocellulosics

    M. TakadaS. Kajita

    46th annual Symposium on Biomaterials, Fuels, and Chemicals  2024.4 

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  • Mechanisms of high enzymatic saccharification efficiency in poplar with highly expression of feruloyl-CoA 6′-hydroxylase gene

    Naning Wang, Masatsugu Takada, Shingo Sakamoto, Naofumi Kamimura, Mikiko Uesugi, Eiji Masai, Nobutaka Mitsuda, John Ralph, Shinya Kajita

    2024.9 

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  • Modification of lignin photoluminescence properties through the incorporation of scopoletin by overexpression of FERULOYL-COA6’HYDROXYLASE1 in transgenic hybrid aspen

    Shota Horinouchi, Naning Wang, Masatsugu Takada, Mikiko Uesugi, Shinya Kajita

    2024.9 

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  • Photoluminescence properties of lignin-derived products obtained by subcritical phenol treatment of Japanese cedar and beech

    Yuki Yamamoto, Masatsugu Takada, Shinya Kajita

    2024.9 

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  • S/G比が段階的に異なるポプラから単離したリグニンの発光特性

    岡村 忠彦, 寺崎 昌海, 髙田 昌嗣, 梶田 真也

    第75回 日本木材学会年次大会  2025.3 

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  • S/G比が段階的に異なるポプラを用いたリグニン基本骨格が発光特性に及ぼす影響

    岡村 忠彦, 寺崎 昌海, 髙田 昌嗣, 梶田 真也

    第20回バイオマス科学会議  2024.12 

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  • Structural analysis and degradation behavior of lignin in “Sekizaisou”, a natural mulberry mutant wood, during White Rot Fungi pretreatment

    James Paul, T. Madigal, Masatsugu Takada, Shinya Kajita

    2nd International Lignin Symposium  2024.9 

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  • The impact of syringyl to guaiacyl ratio on lignin higher-ordered structure in transgenic poplars

    Masami Terasaki, Masatsugu Takada, Mikiko Uesugi, Shinya Kajita

    2024.9 

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  • スギの亜臨界フェノール処理で得られるリグニン由来物の発光特性解析

    山本雄貴, 髙田昌嗣, 梶田真也

    第74回日本木材学会大会  2024.3 

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  • Photoluminescence properties and photo-reactivity of scopoletins-incorporated hybrid aspen lignin with overproduction of feruloyl-CoA 6'-hydroxylase

    2024.8 

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  • ポプラにおけるCINNAMATES 2’-HYDROXYLASEの過剰発現がリグニンの分子構造と発光特性に及ぼす影響

    堀之内翔太, 王娜寧, 髙田昌嗣, 上杉幹子, 梶田真也

    第74回日本木材学会大会  2024.3 

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  • リグニンの利活用に向けた構造・分布の不均一性の理解 Invited

    髙田 昌嗣

    第16回木質科学シンポジウム  2024.6 

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  • リグニン基本骨格が段階的に異なる遺伝子組換えポプラを用いた水熱処理におけるリグニン分解挙動の比較

    寺崎昌海, 髙田昌嗣, 上杉幹子, 梶田真也

    第74回日本木材学会大会  2024.3 

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  • 亜臨界フェノール処理で得られるスギリグニン由来物の発光特性解析

    山本雄貴, 髙田昌嗣, 梶田真也

    COI-NEXTカーボンネガティブの限界に挑戦する炭素耕作拠点会議  2024.3 

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  • 多様なリグノセルロースから単離したリグニンの発光特性解析

    藤牧 圭, 髙田 昌嗣, 梶田 真也

    第75回 日本木材学会年次大会  2025.3 

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  • 種々リグノセルロースから単離した天然リグニンの溶液中での発光特性

    藤牧 圭, 髙田 昌嗣, 梶田 真也

    第20回バイオマス科学会議  2024.12 

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  • CINNAMATES 2’-HYDROXYLASEの過剰発現がポプラリグニンの分子構造と発光特性に及ぼす影響

    堀之内翔太, 王娜寧, 髙田昌嗣, 上杉幹子, 梶田真也

    COI-NEXTカーボンネガティブの限界に挑戦する炭素耕作拠点会議  2024.3 

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  • 遺伝子組換え技術でリグニン分子に導入したスコポレチン構造が発光特性に及ぼす影響

    堀之内 翔太, 王 娜寧, 上杉 幹子, 髙田 昌嗣, 梶田 真也

    第75回 日本木材学会年次大会  2025.3 

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Awards

  • 第2回 国際交流奨励賞

    2024.3   日本木材学会  

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Research Projects

  • 樹木細胞壁への展開を志向した多糖類マトリックス中のリグニン発光特性の解明

    2025.2 - 2028.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    高田 昌嗣

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    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

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  • リグニン高次構造の解明と革新的発光材料の創製

    2023.4 - 2026.3

    国立研究開発法人 科学技術振興機構  2022年度 創発的研究支援事業 

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    Authorship：Principal investigator 

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  • 高機能発光リグニンの創製に向けた消光機構の解明

    2023.4 - 2024.3

    矢崎科学技術振興記念財団  奨励研究 

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    Authorship：Principal investigator 

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  • 高機能発光リグニンの創製に向けた消光数理モデルの開発

    2023.4 - 2024.3

    一般社団法人ヤンマー資源循環支援機構 

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    Authorship：Principal investigator 

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  • Stereochemical control of luminophore distances between lignin by solvolysis using thermochemical treatment

    2022.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists  Grant-in-Aid for Early-Career Scientists

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

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Other

  • The 10th Global Young Scientists Summit 2022, Singapore

    2022.1

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Teaching Experience (On-campus)

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Teaching Experience

  • 基礎技術演習II

    2022.10 Institution：Tokyo University of Agriculture and Technology

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Social Activities

  • 乾式メカニカル処理による難溶解性リグニンの可溶化

    Role(s)： Appearance

    JST  新技術説明会  2024.9

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  • Key Scientific Article "The pretreatment of softwood chips and pellets by alkali sulfonation and thermomechanical pulping"

    2023.1

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    Type：Newspaper, magazine

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  • Biomass energy towards Zero Carbon Energy

    Role(s)： Panelist

    2022 Japan-China High-level Scientist Forum: Energy Transition Session  2022.6

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  • リグニンからの天然有機発光材料の創製

    Role(s)： Appearance

    京都大学  第２回大学発シーズマッチングセミナー 〜機能性材料特集〜  2021.12

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    Type：Lecture

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  • カナダでの研究生活 〜日本との違い〜 + Online Journal Club に関する議論

    Role(s)： Appearance

    6th IAESREC Science Cafe  2020.6

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    Type：Science cafe

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  • 木からケミカルスを創ろう ～謎多きリグニンへの挑戦～

    Role(s)： Lecturer

    京都大学高大連携事業・女子学院高等学校  2017.12

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    Type：Visiting lecture

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  • 木からケミカルスを創ろう ～謎多きリグニンへの挑戦～

    Role(s)： Lecturer

    京都大学高大連携事業・近大附属高等学校  2017.11

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    Type：Visiting lecture

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  • トウモロコシの芯からバイオ燃料を創ろう

    Role(s)： Lecturer

    京都大学高大連携事業・大阪国際大和田高等学校  2017.11

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    Type：Visiting lecture

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  • 木からガソリンを創る！ 坂志朗, 河本晴雄, 南英治, R.ハリファラ, 髙田昌嗣

    Role(s)： Appearance

    京都大学アカデミックデイ2017  2017.9

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    Type：Citizen’s meeting/Assembly

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  • トウモロコシの芯からバイオ燃料を創ろう

    Role(s)： Lecturer

    京都大学高大連携事業・桂高等学校  2017.9

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    Type：Visiting lecture

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Academic Activities

  • 2021 Ajou-Kyoto-Zhejiang Joint Symposium on Energy Science

    Role(s)： Panel moderator, session chair, etc.

    2021.11

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    Type：Competition, symposium, etc. 

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